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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (04): 720-726.doi: 10.3724/SP.J.1006.2013.00720

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Time-Course Metabolic Profiling in Rice under Low Temperature Treatment

ZHAO Xiu-Qin,ZHANG Ting,WANG Wen-Sheng,ZHANG Fan,ZHU Ling-Hua,FU Bin-Ying*,LI Zhi-Kang   

  1. Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Shenzhen Institute of Breeding and Innovation, Chinese Academy of Agricultural Sciences, Shenzhen 518083, China?
  • Received:2012-07-23 Revised:2012-11-16 Online:2013-04-12 Published:2013-01-28
  • Contact: 傅彬英, E-mail: fuby@caas.net.cn, Tel: 010-82106698 E-mail:cass518@yahoo.com.cn

Abstract:

Rice seedling growth is inhibited greatly by the occurrence of low temperature. Characterization of the dynamic changes in rice metabolite under chilling stress will contribute to the understanding of physiological mechanism responsive to abiotic stresses. At the present study, the time-course metabolic profiling of the rice variety, IR64, under low temperature was investigated with GC-MS technique. The results showed that the contents of the oxalic acid, pentonic acid-1,4-lactone, trehalose and salicylic acid increased greatly at the early chilling stress, while phenylalanine, proline, glutamicate, serine, threonine, asparticate, valine, xylitol, allantoin, and rhamnose were induced significantly by the low temperature treatment to keep the osmotic homeostasis. Further dynamic analysis of metabolites indicated that different sets of metabolites were involved in the chilling stress response and the subsequent recovery process. All these results provide a basis for further elucidation of molecular physiological mechanism in rice chilling tolerance.

Key words: Rice, Metabolic Profiling, Low temperature

[1]Boyer J S. Plant productivity and environment. Science, 1982, 218: 443–448



[2]Suzuki N, Mittler R. Reactive oxygen species and temperature stresses: a delicate balance between signaling and destruction. Physiol Plant, 2006, 126: 45–51



[3]Chinnusamy V, Zhu J, Zhu J K. Cold stress regulation of gene expression in plants. Trends Plant Sci, 2007, 12: 444–451



[4]Seki M, Narusaka M, Abe H, Kasuga M, Yamaguchi-Shinozaki K, Carninci P, Hayashizaki Y, Shinozaki K. Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray. Plant Cell, 2001, 13: 61–72



[5]Fowler S, Thomashow M F. Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell, 2002, 14: 1675–1690



[6]Kreps J A, Wu Y, Chang H S, Zhu T, Wang X, Harper J F. Transcriptome changes for Arabidopsis in response to salt, osmotic and cold stress. Plant Physiol, 2002, 130: 2129–2141



[7]Cook D, Fowler S, Fiehn O, Thomashow M F. A prominent role for the CBF cold response pathway in configuring the low-temperature metabolome of Arabidopsis. Proc Natl Acad Sci USA, 2004, 101: 15243–15248



[8]Sakuma Y, Liu Q, Dubouzet J G, Abe H, Shinozaki K, Yamaguchi-Shinozaki K. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem Biophys Res Commun, 2002, 290: 998–1009



[9]Kaplan F, Kopka J, Haskell D W, Zhao W, Schiller K C, Gatzke N, Sung D Y, Guy C L. Exploring the temperature-stress metabolome of Arabidopsis. Plant Physiol, 2004, 136: 4159–4168



[10]Fiehn O. Metabolomics—the link between genotypes and phenotypes. Plant Mol Biol, 2002, 48: 155–171



[11]Yoshiba Y, Kiyosue T, Nakashima K, Kazuko Y S, Shinozakj K. Regulation of levels of proline as an osmolyte in plants under water stress. Plant Cell Physiol, 1997, 38: 1095–1102



[12]Smirnoff N. The function and metabolism of ascorbic acid in plants. Ann Bot, 1996, 78: 661–669



[13]Qiu D-Y(邱德有), Huang L-Q(黄璐琦). Metabolomics—an important part of functional genomics. Mol Plant Breed (分子植物育种), 2004, 2(2): 165–177 (in Chinese with English abstract)



[14]Hall R D. Plant metabolomics: from holistic hope, to hype, to hot topic. New Phytol, 2006, 169: 453–468



[15]Lu H-M(卢红梅), Liang Y-Z(梁逸曾). The development of analytical technologies and data mining in metabolomics. J Instrum Anal (分析测试学报), 2008, 27: 325–331 (in Chinese with English abstract)



[16]Roessner U, Wagner C, Kopka J, Trethewey R, Willmitzer L. Technical advance: simultaneous analysis of metabolites in potato tuber by gas chromatography-mass spectrometry. Plant J, 2000, 23: 131–142



[17]Tarpley L, Duran A L, Kebrom T H, Sumner L W. Biomarker metabolites capturing the metabolite variance present in a rice plant developmental period. BMC Plant Biol, 2005, 5: 8



[18]Schauer N, Semel Y, Roessner U, Gur A, Balbo I, Carrari F, Pleban T, Bruedigam C, Kopka J, Willmitzer L, Zamir D, Fernie A R. Comprehensive metabolic profiling and phenotyping of interspecific introgression lines for tomato improvement. Nat Biotechnol, 2006, 24: 447–454



[19]Meyer R C, Steinfath M, Lisec J, Becher M, Hanna W W, Fiehn O, Eckardt A, Willmitzer L, Selbig J, Altmann T. The metabolic signature related to high plant growth rate in Arabidopsis thaliana. Proc Natl Acad Sci USA, 2007, 104: 4759–4764



[20]Hirai M Y, Mitsuru Y, Dayan B G, Shigehiko K, Kimura T, Awazuhara M, Masanori A. Integration of transcriptomics and metabolomics for understanding of global responses to nutritional stresses in Arabidopsis thaliana. Proc Natl Acad Sci USA, 2004, 101: 10205–10210



[21]Nikiforova V J, Bielecka M, Gakie` re B, Krueger S, Rinder J, Kempa S, Morcuende R, Scheible W R, Hesse H, Hoefgen R. Effect of sulfur availability on the integrity of amino acid biosynthesis in plants. Amino Acids, 2006, 30, 173–183



[22]Zuther E, Koehl K, Kopka J. Comparative metabolome analysis of the salt response in breeding cultivars of rice. In: Jenks M A, Hasegawa P M, Jain S M, eds. Advances in molecular breeding toward drought and salt tolerance crops. New York: Springer-Verlag, 2007. pp 285–315



[23]Armengaud P, Sulpice R, Miller A J, Stitt M, Amtmann A, Gibon Y. Multilevel analysis of primary metabolism provides new insights into the role of potassium nutrition for glycolysis and nitrogen assimilation in Arabidopsis roots. Plant Physiol, 2009, 150: 772–785



[24]Gupta A K, Kaur N. Sugar signalling and gene expression in relation to carbohydrate metabolism under abiotic stresses in plants. J Biosci, 2005, 30: 761–776



[25]Wingler A, Roitsch T. Metabolic regulation of leaf senescence: interactions of sugar signalling with biotic and abiotic stress responses. Plant Biol, 2008, 10: 50–62



[26]Widodo, Patterson J H, Newbigin E, Tester M, Bacic A, Roessner U. Metabolic responses to salt stress of barley (Hordeum vulgare L.) cultivars, Sahara and Clipper, which differ in salinity tolerance. J Exp Bot, 2009, 60: 4089–4103



[27]Yun K Y, Park M R, Mohanty B, Herath V, Xu F, Mauleon R, Wijaya E, Bajic V B, Bruskiewich R, de Los Reyes BG. Transcriptional regulatory network triggered by oxidative signals configures the early response mechanisms of japonica rice to chilling stress. BMC Plant Biol, 2010, 10: 16



[28]Institute of Plant Physiology, Chinese Academy of Sciences (中国科学院上海植物生理研究所) ed. Modern Laboratory Manual of Plant Physiology (现代植物生理学实验指南). Beijing: Science Press, 1998 (in Chinese)



[29]Roessner U, Luedemann A, Brust D, Fiehn O, Linke T, Willmitzer L, Ferniea A R. Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systems. Plant Cell, 2001, 13: 11–29



[30]Bowne J B, Erwin T A., Juttner J, Schnurbusch T, Langridge P, Bacic A. Roessner U. Drought responses of leaf tissues from wheat cultivars of differing drought tolerance at the metabolite level. Mol Plant, 2012, 5: 418–429



[31]Valpuesta V, Botella M A. Biosynthesis of L-ascorbic acid in plants: new pathways for an old antioxidant. Trends Plant Sci, 2004, 9:573-577



[32]Metraux J P, Signer H, Ryals J, Ward E, Wyss-Benz M, Gaudin J, Raschdorf K, Schmid E, Blum W, Inverardi B. Increase in salicylic acid at the onset of systemic acquired resistance in cucumber. Science, 1990, 250: 1004–1006



[33]Schmelz E A, Engelberth J, Alborn H T, O’Donnell P, Sammons M, Toshima H, Tumlinson J H III. Simultaneous analysis of phytohormones, phytotoxins, and volatile organic compounds in plants. Proc Natl Acad Sci USA, 2003, 100: 10552–10557



[34]Garg K, Kim J K, Owens T G, Ranwala A P, Choi Y D, Kochian L V, Wu J. Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses. Proc Natl Acad Sci USA, 2002, 99: 15898–15903



[35]Morsy M R, Jouve L, Hausman J F, Hoffmann L, Stewart J M. Alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice (Oryza sativa L.) genotypes contrasting in chilling tolerance. J Plant Physiol, 2007, 164: 157–167
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