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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (05): 878-884.doi: 10.3724/SP.J.1006.2013.00878

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Cytochemical Observation of Fertile and Sterile Anthers of Thermo-Sensitive Genic Male-Sterile Wheat

LI Dong-Xiao,LI Gan,FENG Su-Wei,RU Zhen-Gang*   

  1. Henan Institute of Science and Technology, Xinxiang 453000, China
  • Received:2012-10-12 Revised:2012-12-13 Online:2013-05-12 Published:2013-02-19
  • Contact: 茹振钢, E-mail: ruzghist@gmail.com

Abstract:

Cytochemical observation of fertile and sterile anthers of thermo-sensitive genic male-sterile wheat (Triticum astivum L.) indicated that lipids in both fertile and sterile anthers were very few during their development. Starches in both anthers were few too before microspore division. In fertile anthers, after microspore division and forming a bi-cellular pollen grain, the big vacuole in vegetative cell of pollen was disintegrated and disappeared, and the cytoplasmic inclusion and starch grains increased persistently. The mature pollen accumulated many starch grains. In sterile anthers, the structure of pollen was similar to that of fertile pollen before microspore division, without much difference between them. After forming a bi-cellular pollen, however, the big vacuole in vegetative cell was not disintegrated and disappeared, the cytoplasmic inclusion decreased, and the accumulated starches in pollen also disappeared. Finally, the pollen grains aborted with its cytoplasmic inclusion disintegration in bi-cellular state. Pollen abortion in this male sterile wheat is related to starch metabolism.

Key words: Wheat, Male sterility, Pollen, Anther, Starch

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