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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (05): 935-942.doi: 10.3724/SP.J.1006.2013.00935

• RESEARCH NOTES • Previous Articles     Next Articles

Identification of Low Phytic acid Maize Germplasm and Primary Screening of Its Molecular Markers

GAO Qing-Hua1,MENG Yi-Jiang1,ZHANG Cui1,JIA Meng1,LIU Zhao1,HOU Ming-Ming1,JIN De-Min2,LI Xue-Jiao1,NIU Dong-Dong1,MIAO Liu-Yang1,GUO Le-Qun2,DOU Shi-Juan1,LIU Li-Juan1,LI Li-Yun1,ZHAI Wen-Xue2,LIU Guo-Zhen1,*   

  1. 1 College of Life Sciences, Agricultural University of Hebei, Baoding 071000, China;2 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2012-10-22 Revised:2013-01-15 Online:2013-05-12 Published:2013-02-19
  • Contact: 刘国振, E-mail: gzhliu@genomics.org.cn, Tel: 0312-7528250

Abstract:

Reducing the content of phytic acid (PA, myo-inositol-1,2,3,4,5,6-hexakis phosphate) is important for improving the nutritional value of maize (Zea mays L.). The identification and application of low phytic acid (lpa) maize germplasm is an economical and effective approach in breeding program. In our previous study, Qi319 was identified as a low phytic acid inbred line. In this study, we found that the content of phytic acid phosphorus in Qi319 kernels is about one fourth of normal maize inbred lines based on quantitative analysis. The characterization of agronomic traits revealed that the germination rate of Qi319 was 75.5%, lower than that of normal inbred lines, however, Qi319 plants grew normally in the field. F2 population was generated by crossing between Qi319 and Lpa241, segregation was found for the content of phytic acid among F2 populations. The segregation ratio of 3:1 confirmed that the lpa trait was determined by a single recessive gene. We then identified two molecular markers (IDP7818 and IDP7635) located on the long arm of chromosome 2 that were co-segregated with low phytic acid loci. This result provides a fundamental basis for marker-assisted maize lpa selection process.

Key words: Maize, Low phytic acid, Marker-assisted selection breeding, Molecular marker, F2 population

[1]Oatway L, Vasanthan T, Helm J H. Phytic acid. Food Rev Int, 2001, 17: 419–431



[2]Lott J N A, Ockenden I, Raboy V, Batten G D. Phytic acid and phosphorus in crop seeds and fruits: a global estimate. Seed Sci Res, 2000, 10: 11–33



[3]Reddy N R, Pierson M D, Sathe S K, Salunlche D K. Phytates in Cereals and Legumes. Florida: CRC Press, 1989



[4]O'Dell B L, De Boland A R, Koirtyohann S R. Distribution of phytate and nutritionally important elements among the morphological components of cereal grains. J Agric Food Chem, 1972, 20: 718–723



[5]Paik I K. Management of excretion of phosphorus, nitrogen and pharmacological level minerals to reduce environmental pollution from animal production. Asian Austral J Anim Sci, 2001, 14: 384–394



[6]Correll D L. The role of phosphorus in the eutrophication of receiving waters: a review. J Environ Qual, 1998, 27: 261–266



[7]Hart M R, Quin B F, Nguyen M L. Phosphorus runoff from agricultural land and direct fertilizer effects. J Environ Qual, 2004, 33: 1954–1972



[8]Reddy K R, Kadlec R H, Flaig E, Gale P M. Phosphorus retention in streams and wetlands: a review. Crit Rev Env Sci Tec, 1999, 29: 83–146



[9]Touchette B W, Burkholder J M. Review of nitrogen and phosphorus metabolism in seagrasses. J Exp Mar Biol Ecol, 2000, 250: 133–167



[10]Lopez H W, Leenhardt F, Coudray C, Remesy C. Minerals and phytic acid interactions: is it a real problem for human nutrition? Int J Food Sci Tech, 2002, 37: 727–739



[11]Chen R, Xue G, Chen P, Yao B, Yang W, Ma Q, Fan Y, Zhao Z, Tarczynski M C, Shi J. Transgenic maize plants expressing a fungal phytase gene. Transgenic Res, 2008, 17: 633–643



[12]Raboy V, Gerbasi P F, Young K A, Stoneberg S D, Pickett S G, Bauman A T, Murthy P P, Sheridan W F, Ertl D S. Origin and seed phenotype of maize low phytic acid 1-1 and low phytic acid 2-1. Plant Physiol, 2000, 124: 355–368



[13]Pilu R, Panzeri D, Gavazzi G, Rasmussen S K, Consonni G, Nielsen E. Phenotypic, genetic and molecular characterization of a maize low phytic acid mutant (lpa241). Theor Appl Genet, 2003, 107: 980–987



[14]Guttieri M, Bowen D, Dorsch J A, Raboy V, Souza E. Identification and characterization of a low phytic acid wheat. Crop Sci, 2004, 44: 418–424



[15]Larson S R, Rutger J N, Young K A, Raboy V. Isolation and genetic mapping of a non-lethal rice (Oryza sativa L.) low phytic acid 1 mutation. Crop Sci, 2000, 40: 1397–1405



[16]Wilcox J R, Premachandra G S, Young K A, Raboy V. Isolation of high seed inorganic P, low-phytate soybean mutants. Crop Sci, 2000, 40: 1601–1605



[17]Shi J, Wang H, Schellin K, Li B, Faller M, Stoop J M, Meeley R B, Ertl D S, Ranch J P, Glassman K. Embryo-specific silencing of a transporter reduces phytic acid content of maize and soybean seeds. Nat Biotechnol, 2007, 25: 930–937



[18]Cerino Badone F, Amelotti M, Cassani E, Pilu R. Study of low phytic acid1-7 (lpa1-7), a new ZmMRP4 mutation in maize. J Hered, 2012, 103: 598–605



[19]Shi J, Wang H, Hazebroek J, Ertl D S, Harp T. The maize low-phytic acid 3 encodes a myo-inositol kinase that plays a role in phytic acid biosynthesis in developing seeds. Plant J, 2005, 42: 708–719



[20]Bregitzer P, Raboy V. Effects of four independent low-phytate mutations on barley agronomic performance. Crop Sci, 2006, 46: 1318–1322



[21]Oltmans S E, Fehr W R, Welke G A, Raboy V, Peterson K L. Agronomic and seed traits of soybean lines with low–phytate phosphorus. Crop Sci, 2005, 45: 593–598



[22]Pilu R, Panzeri D, Cassani E, Cerino Badone F, Landoni M, Nielsen E. A paramutation phenomenon is involved in the genetics of maize low phytic acid1-241 (lpa1-241) trait. Heredity, 2009, 102: 236–245



[23]Shukla S, VanToai T T, Pratt R C. Expression and nucleotide sequence of an INS (3) P1 synthase gene associated with low-phytate kernels in maize (Zea mays L.). J Agric Food Chem, 2004, 52: 4565–4570



[24]Shi J, Wang H, Wu Y, Hazebroek J, Meeley R B, Ertl D S. The maize low-phytic acid mutant lpa2 is caused by mutation in an inositol phosphate kinase gene. Plant Physiol, 2003, 131: 507–515



[25]Israel D W, Kwanyuen P, Burton J W. Genetic variability for phytic acid phosphorus and inorgaic phosphorus in seeds of soybeans in maturity groups V, VI, and VII. Crop Sci, 2006, 46: 67–71



[26]Xu X H, Zhao H J, Liu Q L, Frank T, Engel K H, An G, Shu Q Y. Mutations of the multi-drug resistance-associated protein ABC transporter gene 5 result in reduction of phytic acid in rice seeds. Theor Appl Genet, 2009, 119: 75–83



[27]Feng X, T. Yoshida K. Molecular approaches for producing low-phytic-acid grains in rice. Plant Biotechnol, 2004, 21: 183–189



[28]Kuwano M, Mimura T, Takaiwa F, Yoshida K T. Generation of stable ‘low phytic acid’ transgenic rice through antisense repression of the 1d-myo-inositol 3-phosphate synthase gene (RINO1) using the 18-kDa oleosin promoter. Plant Biotechnol J, 2009, 7: 96–105



[29]Kuwano M, Ohyama A, Tanaka Y, Mimura T, Takaiwa F, Yoshida K. Molecular breeding for transgenic rice with low-phytic-acid phenotype through manipulating myo-inositol 3-phosphate synthase gene. Mol Breed, 2006, 18: 263–272



[30]Kuwano M, Takaiwa F, Yoshida K T. Differential effects of a transgene to confer low phytic acid in caryopses located at different positions in rice panicles. Plant Cell Physiol, 2009, 50: 1387–1392



[31]Kim S I, Andaya C B, Newman J W, Goyal S S, Tai T H. Isolation and characterization of a low phytic acid rice mutant reveals a mutation in the rice orthologue of maize MIK. Theor Appl Genet, 2008, 117: 1291–1301



[32]Andaya C B, Tai T H. Fine mapping of the rice low phytic acid (Lpa1) locus. Theor Appl Genet, 2005, 111: 489–495



[33]Kim S I, Andaya C B, Goyal S S, Tai T H. The rice OsLpa1 gene encodes a novel protein involved in phytic acid metabolism. Theor Appl Genet, 2008, 117: 769–779



[34]Wang X-Y(王雪艳), Wang Z-H(王忠华), Mei S-F(梅淑芳), Hong J(洪隽), Shu Q-Y(舒庆尧), Wu D-X(吴殿星). Brief report on screening maize mutants with high inorganic phosphorus and low phytic acid content. Acta Agric Nucl Sin (核农学报), 2006, 20(1): 404–408 (in Chinese with English abstract)



[35]Wang H(王晖), Chen J-T(陈景堂), Liu L-J(刘丽娟), Chen H(陈浩), Liu G-Z(刘国振). Identification of maize low phytic acid inbred lines and primary study of its genetic mechanism. Acta Agron Sin (作物学报), 2008, 34(1): 95–99 (in Chinese with English abstract)



[36]Ma L(马磊), Li P(李盼), Chen Z(陈哲), Zhao Y-F(赵永锋), Zhu L-Y(祝丽英), Huang Y-Q(黄亚群), Chen J-T(陈景堂). Genetic analysis and identification of maize (Zea mays L.) low phytic acid inbred lines. Sci Agric Sin (中国农业科学), 2011, 44(3): 447–455 (in Chinese with English abstract)



[37]Pilu R, Landoni M, Cassani E, Doria E, Nielsen E. The maize mutation causes a remarkable variability of expression and some pleiotropic effects. Crop Sci, 2005, 45: 2096–2105



[38]Raboy V. Low-phytic-acid grains. Food Nutr Bull, 2000, 21: 423–427



[39]Dorsch J A, Cook A, Young K A, Anderson J M, Bauman A T, Volkmann C J, Murthy P P, Raboy V. Seed phosphorus and inositol phosphate phenotype of barley low phytic acid genotypes. Phytochemistry, 2003, 62: 691–706



[40]Chen P S, Toribara T Y, Warner H. Microdetermination of phosphorus. Anal Chem, 1956, 28: 1756–1758



[41]Dellaporta S L, Wood J, Hicks J B. A plant DNA minipreparation: version II. Plant Mol Biol Rep, 1983, 1: 19–21



[42]Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newberg L A, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1: 174



[43]Strother S. Homeostasis in germinating seeds. Ann Bot, 1980, 45: 217–218



[44]Ye J-C(叶金才). Practices and thoughts on breeding of good maize inbreds and high heterosis hybrids using exogenous germplasm. Shandong Agric Sci (山东农业科学), 2000, (3): 11–13 (in Chinese with English abstract)



[45]Meng Z-D(孟昭东), Guo Q-F(郭庆法), Wang L-M(汪黎明), Liu Z-X(刘治先), Zhang F-J(张发军), Ding Z-H(丁照华), Han J(韩静), Zhang Q-W(张庆伟). Strategies used in the breeding process of high-yield maize hybrid Ludan 981. J Maize Sci (玉米科学), 2003, 11(3): 54–56 (in Chinese with English abstract)
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