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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (11): 1976-1982.doi: 10.3724/SP.J.1006.2013.01976

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Analysis of Soybean Homoserine Kinase (GmHSK) Gene at the Whole Growth Period

WANG Jia-Lin1,**,JIANG Wei1,**,YU Yan4,LIU Chun-Yan2,CHEN Qing-Shan1,*,HU Guo-Hua2,3,*   

  1. 1 College of Agriculture, Northeast Agricultural University, Harbin 150030, China; 2 Land Reclamation Research & Breeding Centre of Heilongjiang, Harbin 150090, China; 3 The National Research Center of Soybean Engineering and Technology, Harbin 150050, China; 4 Development Academy, Jilin Agricultural University, Changchun 130600, China
  • Received:2013-03-06 Revised:2013-06-24 Online:2013-11-12 Published:2013-08-12
  • Contact: 胡国华, E-mail: hugh757@vip.163.com, Tel: 0451-55199475; 陈庆山, E-mail: qshchen@126.com, Tel: 0451-55191945

Abstract:

Homoserine kinase (HSK), the forth enzyme in the aspartate pathway, plays a key role in the biosynthesis for lysine, methionine, threonine, and isoleucine in higher plants. A HSK gene, termed GmHSK was cloned from soybean Williams 82 which contains a 1083 bp single open reading frame (ORF) encoding a 360 amino acid and 37.64 kD protein. The protein shares homology with homoserine kinase from Arabidopsis thaliana. Sequence alignment indicated that GmHSK contained a conserved motif of GHMP kinase superfamily. Phylogenetic analyses suggested that the HSK in plants could be classified into two groups. To have a global view of the dynamic gene expression pattern during the whole growth period, we analyzed the transcript levels in eight different tissues/organs by qRT-PCR. The results showed that the GmHSK gene was expressed in all vegetative organs and reproductive organs with various levels and fluctuated during the plant development. This data provided a versatile resource to understand the regulation of GmHSK gene during the whole growth period in the high protein crop, soybean, and could aid in the transgenic breeding to improve the content and quality of soybean protein.

Key words: Soybean, GmHSK, Expression profile, Whole growth stage

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