Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (04): 622-628.doi: 10.3724/SP.J.1006.2014.00622

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Signal Transduction Pathway of ZmHSF-Like Gene Responding to Different Abiotic Stresses

LI Hui-Cong,LI Guo-Liang,GUO Xiu-Lin*   

  1. Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences / Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China?
  • Received:2013-08-09 Revised:2013-12-13 Online:2014-04-12 Published:2014-02-17
  • Contact: 郭秀林,E-mail: myhf2002@163.com

Abstract:

Based on our previous findings of cloning, expression characteristics and subcellular-location of gene ZmHSF-Like, We analyzed the signal transduction pathway responding to different abiotic stresses. The results showed that the relative expression of gene ZmHSF-Like was up-regulated by H2O2. Up-regulating gene expression by heat shock of 42 ºC was dependent on existence of H2O2, while that PEG-6000 treatment was not. Up-regulating gene expression with ABA was partially dependent on H2O2. The relative expression of gene ZmHSF-Like was up-regulated by Ca2+ too, and chelating Ca2+ with methyleneglycol-bis- (2-aminoethylether)-N,N,N´,N´-tetraacetic acid (EGTA) and blocking Ca2+ intracellular transport with verapamil (Vp) did not decrease gene expression up-regulated by heat shock, PEG and ABA treatments. Those results illustrated that ZmHSF-Like realizes the response to heat shock and ABA through H2O2 signal transduction pathway. While treated with H2O2, the HSP704 gene expression was synchronous with that of ZmHSF-Like gene, HSP704might be the downstream binding protein of ZmHSF-Like in this signal transduction pathway. HSP701, HSP702, and HSPeu701 had the synchronous gene expression with that of ZmHSF-Like gene after Ca2+ treatment, suggesting that they are the downstream binding proteins of ZmHSF-Likeresponding to Ca2+. Those results further indicated that ZmHSF-Like makes response to different stresses through binding different HSPs.

Key words: Maize, ZmHSF-Like, Stresses, H2O2, Ca2+ signal transduction

[1]Nover L, Scharf K D, Gagliardi D, Vergne P, Czarnecka-Verner E, Gurley W B. The HSF world: classification and properties of plant heat stress transcription factors. Cell Stress Chaperones, 1996, 1: 215–223



[2]Schöffl F, Prändl R, Reindl A. Regulation of the heat-shock response. Plant Physiol, 1998, 117: 1135–1141



[3]Nover L, Bharti K, Döring P, Mishra S K, Ganguli A, Scharf K D. Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need. Cell Stress Chaperones, 2001, 6: 177–189



[4]Lee J H, Hübel A, Schöffl F. Derepression of the activity of genetically engineered heat shock factor causes constitutive synthesis of heat shock proteins and increased thermotolerance in transgenic Arabidopsis. Plant J, 1995, 8: 603–612



[5]Nishizawa A, Yabuta Y, Yoshida E, Maruta T, Yoshimura K, Shigeoka S. Arabidopsis heat shock transcription factor A2 as a key regulator in response to several types of environmental stress. Plant J, 2006, 48: 535–547



[6]Charng Y Y, Liu H C, Liu N Y, Chi W T, Wang C N, Chang S H, Wang T T. A heat-induced transcription factor, HsfA2, is required for extension of acquired thermotolerance in Arabidopsis. Plant Physiol, 2007, 143: 251–262



[7]Mishra S K, Tripp J, Winkelhaus S, Tschiersch B, Theres K, Nover L, Scharf K D. In the complex family of heat stress transcription factors, HsfA1 has a unique role as master regulator of thermotolerance in tomato. Genes Dev, 2002, 16: 1555–1567



[8]Yokotani N, Ichikawa T, Kondou Y, Matsui M, Hirochika H, Iwabuchi M, Oda K. Expression of rice heat stress transcription factor OsHsfA2e enhances tolerance to environmental stresses in transgenic Arabidopsis. Planta, 2008, 227: 957–967



[9]Scharf K D, Rose S, Zott W, Schöffl F, Nover L. Three tomato genes code for heat stress transcription factors with a region of remarkable homology to the DNA-binding domain of the yeast HSF. EMBO J, 1990, 9: 4495–4501



[10]Hübel A, Schöffl F. Arabidopsis heat shock factor: isolation and characterization of the gene and the recombinant protein. Plant Mol Biol, 1994, 26: 353–362



[11]Czarnecka-Verner E, Yuan C X, For P C. Isolation and characterization of six heat shock transcription factor cDNA clones from soybean. Plant Mol Biol, 1995, 29: 37–51



[12]Aranda M A, Escaler M, Thomas C L, Maule A J. A heat shock transcription factor in pea is differentially controlled by heat and virus replication. Plant J, 1999, 20: 153–161



[13]Almoguera C, Rojas A, Díaz-Martín J, Prieto-Dapena P, Carranco R, Jordano J. A seed-specific heat-shock transcription factor involved in developmental regulation during embryogenesis in sunflower. Biol Chem, 2002, 277: 43866–43872



[14]Yamanouchi U, Yano M, Lin H X, Ashikari M, Yamada K. A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein. Proc Natl Acad Sci USA, 2002, 99: 7530–7535



[15]Shim D, Hwang J U, Lee J, Lee S, Choi Y, An G, Martinoia E, Lee Y. Orthologs of the class A4 heat shock transcription factor HsfA4a confer cadmium tolerance in wheat and rice. Plant Cell, 2009, 21: 4031–4043



[16]Lin Y X, Jiang H Y, Chu Z X, Tang X L, Zhu S W, Cheng B J. Genome-wide identification, classification and analysis of heat shock transcription factor family in maize. BMC Genomics, 2011, 12: 76–89



[17]Gagliardi D, Breton C, Chaboud A, Vergne P, Dumas C. Expression of heat shock factor and heat shock protein 70 genes during maize pollen development. Plant Mol Biol, 1995, 29: 841–856



[18]Li H C, Li G L, Liu Z H, Zhang H M, Zhang Y M, Guo X L. Cloning, localization and expression of ZmHSF-Like in Zea mays. J Integr Agric, 2013 (accepted)



[19]Zhou R G, Li B, Liu H T, Sun D Y. Progress in the participation of Ca2+-Calmodulin in heat shock signal transduction. Prog Nat Sci, 2009, 19: 1201–1208



[20]Li B, Liu H T, Sun D Y, Zhou R G. Ca2+ and calmodulin modulate DNA-binding activity of maize heat shock transcription factor in vitro. Plant Cell Physiol, 2004, 45: 627–634



[21]Dat J F, Foyer C H, Scott I M. Changes in salicylic acid and antioxidants during induction of thermotolerance in mustard seedlings. Plant Physiol, 1998, 118: 1455–1461



[22]Schett G, Steiner C W, Groger M, Winkler S, Graninger W, Smolen J, Xu Q B, Steiner G. Activation of Fas inhibits heat induced activation of Hsf1 and upregulation of Hsp70. FASEB J, 1999, 13: 833–842



[23]Lee B H, Won S H, Lee H S, Miyaob M, Chung W I, Kimc I J, Joa J. Expression of the chloroplast-localized small heat shock protein by oxidative stress in rice. Gene, 2000, 245: 283–290



[24]Davletova S, Rizhsky L, Liang H J. Cytosolic ascorbate peroxidase1 is a central component of the reactive oxygen gene network of Arabidopsis. Plant Cell, 2005, 17: 268–281



[25]党姣, 蒋明义, 林凡. ABA上调水稻叶片OsHSF基因的表达. 南京农业大学学报, 2010, 33(1): 11–15



Dang J, Jiang M Y, Lin F. ABA up-regulate the expression of OsHSF gene in leaves of rice plants. J Nanjing Agric Univ, 2010, 33(1): 11–15 (in Chinese with English abstract)



[26]郭秀林, 李孟军, 关军锋, 崔四平, 李广敏. PEG胁迫下小麦幼苗ABA与Ca2+/CaM的关系. 作物学报, 2002, 28: 537–540



Guo X L, Li M J, Guan J F, Cui S P, Li G M. The relationship between ABA and Ca2+/CaM in winter wheat seedlings under PEG stress. Acta Agron Sin, 2002, 28: 537–540 (in Chinese with English abstract)



[27]李孟军, 郭秀林, 关军锋, 崔四平, 马春红, 李广敏. 渗透胁迫下外源ABA对小麦幼苗根和叶中ABA及CaM含量的影响. 植物生理学通讯, 2002, 38: 20–21



Li M J, Guo X L, Guan J F, Cui S P, Ma C H, Li G M. Effects of ABA on endogenous ABA and CaM contents of leave and roots of wheat seedlings under osmotic stress. Plant Physiol Commun, 2002, 38: 20–21



[28]李春光, 陈其军, 高新起, 祁碧菽, 陈乃芝, 许守明, 陈珈, 王学臣. 拟南芥热激转录因子AtHsfA2调节胁迫反应基因的表达并提高热和氧化胁迫耐性. 中国科学 C辑 生命科学, 2005, 35: 398-407



Li C G, Chen Q J, Gao X Q, Qi B S, Cheng N Z, Xu S M, Chen J, Wang X C. Heat shock transcription factor AtHsfA2 regulating genes expression related to stresses and increase endurance to heat and oxidation stress in Arabidopsis. Sci China Ser.C Life Sci, 2005, 35: 398–407 (in Chinese)



[29]Liu Z H, Ma Z Y, Liu B H, Guo X L. Changes of cytosolic Ca2+ fluorescence intensity and plasma membrane calcium channels of maize root tip cells under osmotic stress. Plant Physiol Biochem, 2010, 48: 860–865

[1] Liang Jin-Yu, Yin Jia-De, Wang Hong-Li, Zhang Guo-Ping, Hou Hui-Zhi, Dong Bo, Ma Ming-Sheng. Estimation of leaf nitrogen content in dryland forage maize using UAV-based hyperspectral imaging and machine learning [J]. Acta Agronomica Sinica, 2026, 52(6): 1788-1801.
[2] Yang Yang, Chang Shi-Hui, Tian Hong-Li, Yi Hong-Mei, Wang Lu, Ren Jie, Fan Ya-Ming, Liu Ya-Wei, Wang Feng-Ge, Zhao Jiu-Ran. Genetic diversity analysis of nationally approved maize varieties in different ecological regions [J]. Acta Agronomica Sinica, 2026, 52(5): 1352-1364.
[3] Zhang Hong-Rong, Wang Fei-Er, Li Pan, Qiu Hai-Long, Zhu Jing, Zhao Lian-Hao, Nan Yun-You, He Wei, Fan Zhi-Long, Hu Fa-Long, Chai Qiang, Yin Wen. Photosynthetic characteristics of 20% reduced irrigation combined with 25% organic substitution for chemical fertilizer in increasing silage maize yield [J]. Acta Agronomica Sinica, 2026, 52(5): 1487-1500.
[4] Yang Xin-Yu, Cui Wen-Tao, Dilinigeer Alimu, Wang Kai-Xiang, Wu Peng-Hao, Ren Jiao-Jiao. Genome-wide association and genomic selection analysis of the number of leaves above the ear in maize [J]. Acta Agronomica Sinica, 2026, 52(5): 1573-1590.
[5] Han Ya-Xin, He Guan-Hua, Zhang Xiao-Qiong, Zhang Deng-Feng, Li Yong-Xiang, Liu Xu-Yang, Wang Tian-Yu, Li Yu, Zou Hua-Wen, Li Chun-Hui. Identification of maize lateral root density genes resources through integrated RNA-seq and BSA-seq analyses [J]. Acta Agronomica Sinica, 2026, 52(5): 1341-1352.
[6] Sun Shu-Feng, Xu Zhen-Nan, Huang Jia-Xin, Weng Jian-Feng, Li Xin-Hai. Genome-wide identification of the maize MAPK gene family and its response to Fusarium verticillioides infection [J]. Acta Agronomica Sinica, 2026, 52(5): 1291-1308.
[7] Zhang Ning-Ning, Teng Yu-Fei, Ren Na-Na, Wei Xing-Zhuo, Yan Shu-Hao, Fan Ke-Xin, Wang Yong-Hong, Chen Wen-Kang, Zhang Xing-Hua, Zhu Wan-Chao, Xu Shu-Tu, Xue Ji-Quan. Phenotypic evaluation and plasticity analysis of drought resistance in 201 maize inbred lines [J]. Acta Agronomica Sinica, 2026, 52(5): 1309-1325.
[8] Cai Hong-Wei, Yu Ai-Zhong, Jiang Ke-Qiang, Wang Peng-Fei, Wang Yu-Long, Huo Jian-Zhe, Pang Xiao-Neng, Yin Bo, Shang Yong-Pan. Key mechanisms underlying the enhancement of sweet maize yield through partial substitution of chemical fertilizers with organic manure in arid irrigation districts [J]. Acta Agronomica Sinica, 2026, 52(4): 1166-1180.
[9] Tian Hong-Li, Yang Yang, Fan Ya-Ming, Yi Hong-Mei, Guo Dan-Dan, Wang Feng-Ge, Zhao Jiu-Ran. A novel set of tri-allelic variant SNP loci suitable for maize variety identification [J]. Acta Agronomica Sinica, 2026, 52(4): 993-1005.
[10] Guo Xiang-Yang, Tu Liang, Wang Dong, Liu Peng-Fei, Wang An-Gui, Yi Qiang, Ren Hong, Li Gang, Zhu Yun-Fang, Wu Xun, Jiang Yu-Lin, Tian Feng, Chen Ze-Hui. Application and prospects of Suwan germplasm in maize breeding in China [J]. Acta Agronomica Sinica, 2026, 52(3): 655-664.
[11] Meng Cheng, Wang Zhe. Genome-wide identification and expression analysis of the ZmPFK gene family under biotic and abiotic stresses in maize [J]. Acta Agronomica Sinica, 2026, 52(3): 764-779.
[12] Li Xin-Hao, Xing Meng-Ke, Zhou Zi-Hui, Li Si-Ye, Ren Hao, Wang Hong-Zhang, Lai Hua-Jiang. Exogenous melatonin enhances heat tolerance of maize at the seedling stage by coordinating light and dark reactions [J]. Acta Agronomica Sinica, 2026, 52(3): 839-856.
[13] Ma Liang, Ma Lu, Zhang Shu-Yu, Zhang Hui-Min, Wang Ren-Ming, Song Xu-Dong, Zhang Zhen-Liang, Mao Yu-Xiang, Lu Hu-Hua, Chen Guo-Qing, Hao De-Rong, Zhou Guang-Fei. Transcriptome analysis and identification of candidate genes associated with husk number in maize [J]. Acta Agronomica Sinica, 2026, 52(3): 790-801.
[14] Liu Ji-Chang, Li Si-Ye, Li Xue-Ting, Wang Hong-Zhang, Liu Peng, Zhang Ji-Wang, Zhao Bin, Ren Bai-Zhao, Ren Hao. Effects of salt stress on root growth and nutrient absorption efficiency of different salt-tolerant summer maize varieties [J]. Acta Agronomica Sinica, 2026, 52(2): 565-577.
[15] Lin Zi-Qing, Zhong Xing-Yu, Liu Fan, Ren Zi-Ao, Ma Rui, Deng Xiu-Feng, Wang Dong-Wei, Liu Shao-Peng, Chen Kang, Zhang Ming-Cai, Li Zhao-Hu, Zhou Yu-Yi, Duan Liu-Sheng. Development of ultra-high-yield technology for a wheat-maize double cropping system achieving a 2-ton annual grain yield per mu in the coastal plain of Northern Shandong peninsula, China [J]. Acta Agronomica Sinica, 2026, 52(2): 631-643.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!