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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (04): 622-628.doi: 10.3724/SP.J.1006.2014.00622


Signal Transduction Pathway of ZmHSF-Like Gene Responding to Different Abiotic Stresses

LI Hui-Cong,LI Guo-Liang,GUO Xiu-Lin*   

  1. Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences / Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China?
  • Received:2013-08-09 Revised:2013-12-13 Online:2014-04-12 Published:2014-02-17
  • Contact: 郭秀林,E-mail: myhf2002@163.com


Based on our previous findings of cloning, expression characteristics and subcellular-location of gene ZmHSF-Like, We analyzed the signal transduction pathway responding to different abiotic stresses. The results showed that the relative expression of gene ZmHSF-Like was up-regulated by H2O2. Up-regulating gene expression by heat shock of 42 ºC was dependent on existence of H2O2, while that PEG-6000 treatment was not. Up-regulating gene expression with ABA was partially dependent on H2O2. The relative expression of gene ZmHSF-Like was up-regulated by Ca2+ too, and chelating Ca2+ with methyleneglycol-bis- (2-aminoethylether)-N,N,N´,N´-tetraacetic acid (EGTA) and blocking Ca2+ intracellular transport with verapamil (Vp) did not decrease gene expression up-regulated by heat shock, PEG and ABA treatments. Those results illustrated that ZmHSF-Like realizes the response to heat shock and ABA through H2O2 signal transduction pathway. While treated with H2O2, the HSP704 gene expression was synchronous with that of ZmHSF-Like gene, HSP704might be the downstream binding protein of ZmHSF-Like in this signal transduction pathway. HSP701, HSP702, and HSPeu701 had the synchronous gene expression with that of ZmHSF-Like gene after Ca2+ treatment, suggesting that they are the downstream binding proteins of ZmHSF-Likeresponding to Ca2+. Those results further indicated that ZmHSF-Like makes response to different stresses through binding different HSPs.

Key words: Maize, ZmHSF-Like, Stresses, H2O2, Ca2+ signal transduction

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