Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (05): 733-742.doi: 10.3724/SP.J.1006.2015.00733

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Effects of Relay Strip Intercropping Years between Flue-cured Tobacco and Soybean on Rhizospheric Microbes Quantities 

TU Yong1,2,YANG Wen-Yu1,*,LIU Wei-Guo1,YONG Tai-Wen1,JIANG Lian-Qiang3,WANG Xiao-Chun1   

  1. 1 College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China; 2School of Agricultural Science of Xichang College, Xichang 615013, China; 3Liangshan Tobacco Company of Sichuan Province, Xichang 615000, China?
  • Received:2014-10-18 Revised:2015-03-19 Online:2015-05-12 Published:2015-04-01
  • Contact: 杨文钰, E-mail: wenyu.yang@263.net E-mail:tuy-019@163.com

Abstract:

To overcome obstacles from continuous tobacco cropping and improve the yield and quality of flue-cured tobacco, we studied the effects of flue-cured tobacco and soybean relay intercropping years on the population changes of three classifiable microorganism and functional microorganisms related to nitrogen metabolism in rhizospheric soil, and their relationship with the occurrence of major soil-borne disease of flue-cured tobacco in this research. Results showed that the flue-cured tobacco-soybean relay strip intercropping markedly increased the population of bacteria (B), actinomycetes (A), B/F, and A/F, while reduced the fungi (F) quantity in rhizospheric soil. Meanwhile, the flue-cured tobacco-soybean relay intercropping enhanced the quantity of functional microorganisms related to nitrogen metabolism, such as ammonifier, nitrobacteria, nitrite bacteria and free-living nitrogen fixing bacteria. In addition, it alleviated the occurrence level of soil-borne disease of flue-cured tobacco, and the severity indexes of soil-borne disease had significantly negative correlation with the quantities of bacteria and actinomycetes respectively, but were positively correlated with the increasing of fungi. With increasing the number of years for relay intercropping, the quantities of bacteria (B), actinomycetes (A), and value of B/F and A/F, ammonifier, nitrobacteria, nitrite bacteria and free-living nitrogen fixing bacteria were raised largely, while the quantities of the fungi (F) and the denitrifying bacteria were significantly declined, and there was a marked improvement in the relative control efficiency to soil-borne disease of flue-cured tobacco. These effects showed a trend of flue-cured tobacco relay strip intercropping with soybean for three years (A3) > that intercropping for two years (A2) > that intercropping for one year (A1).

Key words: Flue-cured tobacco relay strip intercropping with soybean, Different intercropping years, Rhizospheric soil, Microbes quantities

[1]张继光, 申国明, 张久权, 张忠锋, 石屹, 李世博, 刘海伟, 时鹏. 烟草连作障碍研究进展. 中国烟草科学, 2011, 32(3): 95–99



Zhang J G, Shen G M, Zhang J Q, Zhang Z F, Shi Y, Li S B, Liu H W, Shi P. Advance in continuous cropping problems of tobacco. Chin Tob Sci, 2011, 32(3): 95–99 (in Chinese with English abstract)



[2]王鑫, 刘建新. 黄土高原沟壑区烟草连作对土壤腐殖质性质的影响. 水土保持学报, 2007, 21(6): 126–129



Wang X, Liu J X. Effect of tobacco long-continuous cropping on character of soil humus in gully region of loess plateau. J Soil Water Conserv, 2007, (6): 126–129 (in Chinese with English abstract)



[3]石秋环, 焦枫, 耿伟, 李群平, 时向东. 烤烟连作土壤环境中的障碍因子研究综述. 中国烟草学报, 2009, 15(6): 81–84



Shi Q H, Jiao F, Geng W, Li Q P, Shi X D. An overview on research into factors hindering continuous cropping in flue-cured tobacco. Acta Tab Sin, 2009, 15(6): 81–84 (in Chinese with English abstract)



[4]何川, 刘国顺, 李祖良, 乔保明, 董宁禹, 蒋士君. 连作对植烟土壤有机碳和酶活性的影响及其与土传病害的关系. 河南农业大学学报, 2011, 45: 701–705



He C, Liu G S, Li Z L, Qiao B M, Dong N Y, Jiang S J. Effect of continuous cropping on tobacco soil organic carbon, enzyme activities, and its relationship with soil-borne diseases. J Henan Agric Univ, 2011, 45: 701–705 (in Chinese with English abstract)



[5]张继光, 郑林林, 石屹, 张忠锋, 马兴华, 申国明, 冯俊喜, 田雷. 不同种植模式对土壤微生物区系及烟叶产量与质量的影响. 农业工程学报, 2012, 28(19): 93–102



Zhang J G, Zheng L L, Shi Y, Zhang Z F, Ma X H, Shen G M, Feng J X, Tian L. Effects of different planting patterns on soil microbial community, yield and quality of flue-cured tobacco leaves. Trans CSAE, 2012, 28(19): 93–102 (in Chinese with English abstract)



[6]冯俊喜, 王树声, 石屹, 张继光, 郑林林, 李湘伟, 胡庆辉, 王浩浩. 山东烟区不同种植模式土壤微生物群落特征研究. 中国烟草科学, 2011, 32(2): 38–42



Feng J X, Wang S S, Shi Y, Zhang J G, Zheng L L, Li X W, Hu Q H, Wang H H. Characteristics of soil microbial community under different cropping systems in flue-cured tobacco growing areas of shandong province. Chin Tob Sci, 2011, 32(2): 38–42 (in Chinese with English abstract)



[7]张汉球. 麦烟套种综合利用研究. 安徽农业科学, 2010, 38: 16840–16841



Zhang H Q. Research on the intercropping model of wheat and tobacco. J Anhui Agric Sci, 2010, 38: 16840–16841



[8]刘会忠, 赵中汇, 黎明江, 刘晓昂, 张西仲. 烤烟与小麦套作和地膜覆盖对烤烟马铃薯Y病毒病发病率的影响. 作物研究, 2013, 27(4): 351–354



Liu H Z, Zhao Z H, Li M J, Liu X A, Zhang X Z. Influence of flue-cured tobacco interplanting with wheat and plastic mulching on the disease incidence of flue-cured tobacco potato virus Y. Crop Res, 2013, 27(4): 351–354 (in Chinese with English abstract)



[9]刘建辉, 李俊. 烤烟套种秋玉米高产栽培技术. 云南农业, 2010, (8): 32



Liu J H, Li J. Cultivation technology for high yield about flue-cured tobacco intercropping with corn. Yunnan Agric, 2010(8): 32 (in Chinese)



[10]钱育华. 楚雄市烤烟套种晚秋玉米栽培技术. 云南农业, 2010, (8): 11



Qian Y H. Cultivation technology about flue-cured tobacco intercropping with late autumn maize in Chuxiong city. Yunnan Agric, 2010, (8): 11 (in Chinese)



[11]李明福, 李洪民, 徐宁生, 许建辉, 李玉萍, 李春丽, 马娅. 云南玉溪烤烟套作紫甘薯最佳栽插期试验. 南方农业学报, 2013, (9): 1455–1458



Li M F, Li H M, Xu N S, Xu J H, Li Y P, Li C L, Ma Y. Optimal pianting date of purple sweetpotato intercropped with tobacco. J Southern Agric, 2013, (9): 1455–1458 (in Chinese with English abstract)



[12]田景先, 陈勇, 吕荣, 陈文胜, 薛小平. 套作红苕对烤烟生长及经济效益的影响. 山东农业科学, 2014, 46(2): 59–61



Tian J X, Chen Y, Lü R, Chen W S, Xue X P. Effects of intercropping sweet potato on growth and economic benefit of flue-cured tobacco. Shandong Agric Sci, 2014, 46(2): 59–61(in Chinese with English abstract)



[13]刘丽芳, 唐世凯, 熊俊芬, 郑毅. 烤烟间套作草木樨和甘薯对烟叶含钾量及烟草病毒病的影响. 中国农学通报, 2006, 22(8): 238–241



Liu L F, Tang S K, Xiong J F, Zheng Y. Effects of flue-cured tobacco and sweet clover and sweet potato intercropping on leave potassium contents and virus diseases occurrence of tobacco. Chin Agric Sci Bull, 2006, 22(8): 238–241 (in Chinese with English abstract)



[14]容开定, 何录秋, 张亚. 旱地烤烟免耕直播套种秋大豆栽培技术研究. 耕作与栽培, 2006, (6): 22–23



Rong K D, He L Q, Zhang Y. Research on cultivation technology about flue-cured tobacco intercropping with autumn soybean under no tillage and direct seeding. Gengzuo yu Zaipei, 2006, (6): 22–23 (in Chinese)



[15]尤开勋, 王兴国, 张尚学, 代启兵, 刘国敏, 袁建华, 秦拥政. 高山大蒜-白肋烟套作栽培技术. 中国园艺文摘, 2010, (12): 142–144



You K X, Wang X G, Zhang S X, Dai Q B, Liu G M, Yuan J H, Qian Y Z. Cultivation technology about burley tobacco interplanting with garlic under the alpine. Chin Hort Abst, 2010, (12): 142–144 (in Chinese)



[16]付利波, 王毅, 杨跃, 瞿兴, 左天龙, 耿明建, 苏帆, 洪丽芳. 利用烟田套作调控高肥力土壤烤烟生产. 植物营养与肥料学报, 2005, 11: 128–132



Fu L B, Wang Y, Yang Y, Zhuo X, Zuo T L, Geng M J, Su F, Hong L F. Regulating the growth of flue-cured tobacco by interplanting in fertility soil. Plant Nutr Fert Sci, 2005, 11: 128–132 (in Chinese with English abstract)



[17]唐世凯, 刘丽芳, 李永梅. 烤烟套种甘薯对持续控制烟草病害的影响. 广东农业科学, 2008, (9): 26–28



Tang S K, Liu L F, Li Y M. Effects of flue-cured tobacco intercropping with sweet potato on diseases occurrence of flue-cured tobacco leaves. Guangdong Agric Sci, 2008, (9): 26–28 (in Chinese with English abstract)



[18]刘新晶, 许艳丽, 李春杰, 孟庆杰. 大豆轮作系统对土壤细菌生理菌群的影响. 大豆科学, 2007, 26: 723–727



Liu X J, Xu Y L, Li C J, Meng Q J. Effect of soybean rotation system on the bacterial physiological groups. Soybean Sci, 2007, 26: 723–727 (in Chinese with English abstract)



[19]郑华, 石俊雄, 樊立辉, 黄建国. 菜籽饼与化肥配施对烤烟根际微生物的影响. 中国烟草科学, 2009, 30(6): 46–48



Zheng H, Shi J X, Fan L H, Huang J G. Effects of combined application of rapeseed cake and chemical fertilizer on rhizosphere microbes of flue-cured tobacco. Chin Tob Sci, 2009, 30(6): 46–48 (in Chinese with English abstract)



[20]林先贵. 土壤微生物研究原理与方法. 北京: 高等教育出版社, 2010. pp 29–54



Lin X G. Principle and method of soil microbiology research. Beijing: Higher Education Press, 2010. pp 29–54 (in Chinese)



[21]焦晓丹, 吴凤芝. 土壤微生物多样性研究方法的进展. 土壤通报, 2004, 35: 789–792



Jiao X D, Wu F Z. Progress of the methods for studying soil microbial diversity. Chin J Soil Sci, 2004, 35: 789–792 (in Chinese with English abstract)



[22]赵佳佳, 李季, 杜相革. 不同生产模式对土壤微生物种群数量的影响. 华北农学报, 2011, (6): 220–224



Zhao J J, Li J, Du X G. Dynamics of soil microbial community in the experiment of organic green and conventional vegetable production systems. Acta Agric Boreali-Sin, 2011, (6): 220–224 (in Chinese with English abstract)



[23]李妮, 宋付朋, 杨岩, 李九五, 赵军, 曲海滨. 轮作年限对大蒜根际土壤微生物群落的影响. 水土保持学报, 2013, 27(5): 210–218



Li N, Song F P, Yang Y, Li J W, Zhao J, Qu H B. Effects of rotation years on rhizosphere soil microbial sommunities of garlic. J Soil Water Conserv, 2013, 27(5): 210–218 (in Chinese with English abstract)



[24]林雁冰, 薛枭宏, 颜霞. 不同栽培模式下玉米根系对土壤微生物区系的影响. 西北农林科技大学学报, 2008, 36(12): 102–107



Lin Y B, Xue X H, Yan X. Effects of the maize root on soil microbial flora under different cultivation patterns. J Northwest A&F Univ (Nat Sci Edn), 2008, 36(12): 102–107 (in Chinese with English abstract)



[25]李忠俊, 罗会斌, 龙友华, 李忠. 烟草根际微生物区系分析. 栽培与耕作, 2012, (3): 35–39



Li Z J, Luo H B, Long Y H, Li Z. Floristic analysis of tobacco rhizosphere microorganisms. Gengzuo yu Zaipei, 2012, 3: 35–39 (in Chinese)



[26]Prather T S, Lanini W T, Orloff S, Vargas R, Schmierer J L, Hembree K, Canevari W M, Mueller S, Bendixen W, Krebill-Prather R L. Interplanting grasses into alfalfa controls weeds in older stands. California Agric, 2000, 54: 37–41



[27]胡亚林, 汪思龙, 颜绍馗. 影响土壤微生物活性与群落结构因素研究进展. 土壤通报, 2006, 37(1): 70–176



Hu Y L, Wang S L, Yan S K. Research advances on the factors influencing the activity and community structure of soil microorganism. Chin J Soil Sci, 2006, 37(1): 170–176 (in Chinese with English abstract)



[28]朱丽霞, 张家恩, 刘文高. 根系分泌物与根际微生物相互作用研究综述. 生态环境, 2003, 12 (1): 102–105



Zhu L X, Zhang J E, Liu W G. Review of studies on interactions between root exudates and rhizopheric microorganisms. Ecol Environt, 2003, 12(1): 102–105 (in Chinese with English abstract)



[29]苏本营, 陈圣宾, 李永庚, 杨文钰. 间套作种植提升农田生态系统服务功能. 生态学报, 2013, 33: 4505–4514



Su B Y, Chen S B, Li Y G, Yang W Y. Intercropping enhances the farmland ecosystem services. Acta Ecol Sin, 2013, 33: 4505–4514 (in Chinese with English abstract)



[30]殷全玉, 王岩, 赵铭钦, 张小全. 我国植烟土壤微生物研究进展. 中国烟草科学, 2009, 30(1): 73–77



Yin Q Y, Wang Y, Zhao M Q, Zhang X Q. Research progress on microorganism in tobacco planting soil. Chin Tob Sci, 2009, 30(1): 73–77 (in Chinese with English abstract)



[31]刘训理, 王超, 吴凡, 薛东红, 陈凯. 烟草根际微生物研究. 生态学报, 2006, 26: 552–557



Liu X L, Wang C, Wu F, Xue D H, Chen K. Studies on tobacco rhizosphere microbes. Acta Ecol Sin, 2006, 26: 552–557 (in Chinese with English abstract)



[32]陈良存, 阮联平, 张惠军, 陈明, 杨力. 不同植烟条件下烤烟根际微生物区系的差异. 安徽农业科学, 2012, 40: 11268–11269



Chen L C, Ruan L P, Zhang H J, Chen M, Yang L. Difference of microorganism flora in flue-cured tobacco rhizosphere under different tobacco planting conditions. J Anhui Agri Sci, 2012, 40: 11268–11269 (in Chinese with English abstract)



[33]王超, 吴凡, 刘训理, 刘兵. 不同肥力条件下烟草根际微生物的初步研究. 中国烟草科学, 2005, (2): 12–14



Wang C, Wu F, Liu X L, Liu B. Tobacco rhizosphere microorganism in different fertility of soil. Chin Tob Sci, 2005, (2): 12–14 (in Chinese with English abstract)



[34]张翼, 曹仕明, 史俊, 孟贵星, 谭军, 赵秀云. 烟草土壤微生物与土壤酶活性分析. 湖北农业科学, 2012, 51: 4229–4232



Zhang Y, Cao S M, Shi J, Meng G X, Tan J, Zhao X Y. Analysis on microorganisms and enzymes activity in soil of tobacco field. Hubei Agric Sci, 2012, 51: 4229–4232 (in Chinese with English abstract)

No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!