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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (10): 1419-1428.doi: 10.3724/SP.J.1006.2016.01419

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Interaction of Soybean 14-3-3 Proteins with Transcription Factor GmMYB173

DONG Meng1,2, GAO You-Fie2, HAN Tian-Fu2, DONG-FANG Yang1,*, and JIANG Bing-Jun2,*   

  1. 1 Life Science and Technology College, Hebei Normal University of Science & Technology, Qinhuangdao 066000, China; 2 Key Laboratory of Soybean Biology (Beijing), Ministry of Agriculture / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2016-03-15 Revised:2016-06-20 Online:2016-10-12 Published:2016-07-04
  • Contact: 东方阳,E-mail:yang_dongfang@hotmail.com,Tel:13780335864;蒋炳军,E-mail:jiangbingjun@caas.cn,Tel:13910305439 E-mail:dongmeng902@163.com
  • Supported by:

    ThisstudywassupportedbytheSpecialProgramofModernAgro-industryTechnologySystem(CARS-04)andtheAgriculturalScienceandTechnologyInnovationProgramofChineseAcademyofAgriculturalSciences.

Abstract:

14-3-3 proteins, nearly existing in all eukaryotic cells, may regulate many physiological and biochemical processes through interacting with other proteins. As the largest class of transcription factors in plants, MYB gene family is widely involved in plant growth and metabolism regulation. A gene cloned from soybean cultivar Zigongdongdou, and specifically expressed in the nuclear in the subcellular location assay. Sequence analysis showed that there was a binding site of 14-4-4 proteins, namely the pST binding site, in GmMYB173 similar to that in GmMYB176. The nuclear-specific expression of GmMYB173 disappeared when the sequence of the pST binding site was deleted through the splicing by overlap extension PCR. All 14-3-3 proteins from GmSGF14a to GmSGF14p could interact with GmMYB173. Among them, GmSGF14n interacted with GmMYB173 strongest, GmSGF14e and GmSGF14k took second place, which was proved by the β-galactosidase activity analysis. These results suggest that 14-3-3 proteins not only interact with GmMYB173, but also probably regulate its subcellular location. The information provided by this study will facilitate the study of interaction relationship between 14-3-3 proteins and GmMYB173 and its function on the soybean development.

Key words: 14-3-3proteins, GmMYB173, Subcellularlocation, Yeasttwo-hybrid

[1]BrandtJ,Thordal-ChristensenH,VadK,GregersenPL,CollungeDB.Apathogen-inducedgeneofbarleyencodesaproteinshowinghighsimilaritytoaproteinkinaseregulator.PlantJ,1992,2:815–820 [2]LuG,DeLisleAJ,deVettenNC,FerlRJ.Brainproteinsinplants:anArabidopsishomologtoneurotransmitterpathwayactivatorsispartofaDNAbindingcomplex.ProcNatlAcadSciUSA,1992,89:11490–11494 [3]HirschS,AitkenA,BertschU,SollJ.Aplanthomologuetomammalianbrain14-3-3proteinandproteinkinaseCinhibitor.FEBSLett,1992,296:222–224 [4]DeLilleJM,SehnkePC,FerlRJ.TheArabidopsis14-3-3familyofsignalingregulators.PlantPhysiol,2001,126:35–38 [5]RosenquistM,AlsterfjordM,LarssonC,SommarinM.DataminingtheArabidopsisgenomerevealsfifteen14-3-3genes.Expressionisdemonstratedfortwooutoffivenovelgenes.PlantPhysiol,2001,127:142–149 [6]YaoY,DuY,JiangL,LiuJY.Molecularanalysisandexpressionpatternsofthe14-3-3genefamilyfromOryzasativa.JBiochemMolBiol,2007,40:349–357 [7]LiX,DhaubhadelS.Soybean14-3-3genefamily:identificationandmolecularcharacterization.Planta,2011,233:569–582 [8]YaffeMB,RittingerK,VoliniaS,CaronPR,AitkenA,LeffersH,GamblinSJ,SmerdonSJ,CantleyLC.Thestructuralbasisfor14-3-3:phosphopeptidebindingspecificity.Cell,1997,91:961–971 [9]FuH,SubramanianRR,MastersSC.14-3-3proteins:structure,function,andregulation.AnnuRevPharmacolToxicol,2000,40:617–647 [10]SumiokaA,NagaishiS,YoshidaT,LinA,MiuraM,SuzukiT.Roleof14-3-3γinFE65-dependentgenetransactivationmediatedbytheamyloidβ-proteinprecursor.JBiolChem,2005,280:42364–42374 [11]CasarettoJ,HoTH.ThetranscriptionfactorsHvABI5andHvVP1arerequiredfortheabscisicacidinductionofgeneexpressioninbarleyaleuronecells.PlantCell,2003,15:271–284 [12]FoltaKM,PaulAL,MayfieldJD,FerlRJ.14-3-3isoformsparticipateinredlightsignalingandphotoperiodicflowering.PlantSignalBehav,2008,3:304–306 [13]HuberSC,MacKintoshC,KaiserWM.Metabolicenzymesastargetsfor14-3-3proteins.PlantMolBio,2002,50:1053–1063 [14]PaulAL,FoltaKM,FerlRJ.14-3-3proteins,redlightandphotoperiodicflowering:apointofconnection.PlantSignalBehav,2008,3:511–515 [15]HajduchM,GanapathyA,SteinJW,ThelenJJ.Asystematicproteomicstudyofseedfillinginsoybean.Establishmentofhigh-resolutiontwo-dimensionalreferencemaps,expressionprofiles,andaninteractiveproteomedatabase.PlantPhysiol,2005,137:1397–1419 [16]ProuseMB,CampbellMM.TheinteractionbetweenMYBproteinsandtheirtargetDNAbindingsites.BiochimBiophysActa,2012,1819:67–77 [17]陈清,汤浩茹,董晓莉,侯艳霞,罗娅,蒋艳,黄琼瑶.植物Myb转录因子的研究进展.基因组学与应用生物学,2009,28:365–372 ChenQ,TangHR,DongXL,HouYX,LuoY,JiangY,HuangQY.ProgressintheStudyofPlantMybTranscriptionFactors.GenoApplBiol,2009,28:365–372(inChinesewithEnglishabstract) [18]LiaoY,ZouHF,WangHW,ZhangWK,MaB,ZhangJS,ChenSY.SoybeanGmMYB76,GmMYB92,andGmMYB177genesconferstresstoleranceintransgenicArabidopsisplants.CellRes,2008,18:1047–1060 [19]李晓薇.大豆两个MYB转录因子基因的克隆及其功能分析.吉林大学博士学位论文,吉林长春,2011 LiXW.CloningandCharacterizationofTwoMYBTranscriptionFactorGenesfromSoybean.PhDDissertationofJilinUniversity,Changchun,China,2011(inChinesewithEnglishabstract) [20]LiuYF,LiQT,LuX,SongQX,LamSM,ZhangWK,MaB,LinQ,ManWQ,DuWG,ShuiGH,ChenSY,ZhangJS.SoybeanGmMYB73promoteslipidaccumulationintransgenicplants.BMCPlantBiol,2014,14:1 [21]ChenL,BernhardtA,LeeJ,HellmannH.IdentificationofArabidopsisMYB56asaNovelSubstrateforCRL3(BPM)E3Ligases.MolPlant,2015,8:242–250 [22]LiuL,ZhangJ,AdrianJ,GissotL,CouplandG,YuD,TurckF.ElevatedLevelsofMYB30inthephloemacceleratefloweringinArabidopsisthroughtheregulationofFLOWERINGLOCUST.PloSOne,2014,9:e89799 [23]YiJ,DerynckMR,LiX,TelmerP,MarsolaisF,DhaubhadelS.Asingle-repeatMYBtranscriptionfactor,GmMYB176,regulatesCHS8geneexpressionandaffectsisoflavonoidbiosynthesisinsoybean.PlantJ,2010,62:1019–1034 [24]LiX,ChenL,DhaubhadelS.14-3-3proteinsregulatetheintracellularlocalizationofthetranscriptionalactivatorGmMYB176andaffectisoflavonoidsynthesisinsoybean.PlantJ,2012,71:239–250 [25]高友菲,岳岩磊,蒋炳军,韩天富.大豆GmFT2a启动子InDel区结合蛋白的筛选.中国油料作物学报,2015,37:27–34 GaoYF,YueYL,JiangBJ,HanTF.ScreeningofproteinbindingtoGmFT2apromoterindelregioninsoybean.ChinJOilCropSci,2015,37:27–34(inChinesewithEnglishabstract) [26]曲梦楠.大豆GmFT2b基因的功能分析.中国农业科学院硕士学位论文,北京,2014 QuMN.FunctionAnalysisofGmFT2bGeneinGlyminemax.MSThesisofChineseAcademyofAgriculturalSciences,Beijing,China,2014(inChinesewithEnglishabstract) [27]邢浩然,刘丽娟,刘国振.植物蛋白质的亚细胞定位研究进展.华北农学报,2006,21(增刊2):1–6 XingHR,LiuLJ,LiuGZ.AdvancementofProteinSubcellularLocalizationinPlant.ActaAgricBoreali-Sin,2006,21(S2):1–6(inChinesewithEnglishabstract) [28]刘海燕,冯冬茹,刘兵,何炎明,王宏斌,王金发.农杆菌介导的MpASR蛋白在洋葱表皮细胞的定位研究.热带亚热带植物学报,2009,17:218–222 LiuHY,FengDR,LiuB,HeYM,WangHB,WangJF.StudiesonsubcellularlocalizationofMpASRinonionepidermalcellsmediatedbyAgrobacterium.JTrop&SubtropBot,2009,17:218–222(inChinesewithEnglishabstract) [29]JinH,XuG,MengQ,HuangF,YuD.GmNAC5,aNACtranscriptionfactor,isatransientresponseregulatorinducedbyabioticstressinsoybean.SciWorldJ,2013,2013:768–972 [30]NanH,CaoD,ZhangD,LiY,LuS,TangL,YuanX,LiuB,KongF.GmFT2aandGmFT5aredundantlyanddifferentiallyregulatefloweringthroughinteractionwithandupregulationofthebZIPtranscriptionfactorGmFDL19insoybean.PloSOne,2014,9:e97669

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