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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (08): 1196-1204.doi: 10.3724/SP.J.1006.2017.01196

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Relationship of Sink Activity with Endogenous Hormones and Polyamine Contents in Inferior Kernels of Maize

WANG Zhi-Gang1,**,LIANG Hong-Wei1,2,**,GAO Ju-Lin1,*,YU Xiao-Fang1,SUN Ji-Ying1,SU Zhi-Jun1,HU Shu-Ping1,YU Shao-Bo1,LI Ya-Jian1,WEI Shu-Li1,YANG Zhe1   

  1. 1 College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010019, China; 2 Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China
  • Received:2016-12-05 Revised:2017-04-20 Online:2017-08-12 Published:2017-05-08
  • Contact: Gao junlin, E-mail: nmgaojulin@163.com E-mail:imauwzg@163.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31301265), the State Program of China (2016YFD0300103), the Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region (NJYT-14-B04), and the National Key Technology Support Program of China (2013BAD07B04).

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Abstract:

Sink activity at blister stage of maize is the primary limiting factor of the abortion and/or filling stagnation of inferior kernels. Clarifying the effect of endogenous hormone and polyamine contents on sink activity of inferior kernels in maize is of great importance for regulating inferior kernels in crowding maize colony. In this present study, two typical commercial maize hybrids Zhengdan 958 and Xianyu 335 were planted in 2014 and 2015. At silking stage, two pollination treatments, incomplete pollination (IcP) and complete pollination (CP), were imposed to each cultivar. The objective of incomplete pollination, in which the pollinated filaments within the basal region of ear was decreased by hand-cutting, was to stimulate the inferior kernel setting in the apical region of ear, where the inferior kernels should be aborted or filling-stagnated in complete pollination. The endogenous hormone and polyamine contents were compared between IcP and CP treatments during blister stage, and the relationship of sink activity with endogenous hormone and/or polyamine contents was analyzed. There was no remarkable effect of hybrids and years on sink activity, endogenous hormone and polyamine of kernels. The soluble acid invertase activity of IcP was significantly higher than that of CP, by 13.5% on an average and 21.8% at the maximum. There was no significant difference in contents of Z+ZR, IAA, GA3, and ABA of the inferior kernels between IcP and CP treatments during blister stage. The polyamine content of IcP was significantly higher than that of CP, but the ethylene producing rate was just the opposite. SAI activity correlated with polyamine content positively, but with ethylene release rate negatively, moreover, ethylene release rate showed negative correlation with polyamine content. The results suggested that, during the blister stage of maize, the contents of Z+ZR, IAA, GA3, and ABA of inferior kernels have nothing to do with SAI activity. SAI activity of inferior kernels is mainly affected by polyamine and ethylene, which is promoted by polyamine content and inhibited by ethylene release rate. The balance between polyamine and ethylene determines the result of inferior kernel development, which is radically regulated by assimilate supply of maize plant.

Key words: Maize, Inferior kernel, Sink activity, Hormone, Polyami

[1]Wang Z G, Gao J L, Ma B L. Concurrent improvement in maize yield and nitrogen use efficiency with integrated agronomic management strategies. Agron J, 2014, 106: 1243–1250
[2]孙国伟, 张风路, 郭江, 赵久然, 郭景伦. 对玉米籽粒败育原因的再认识. 玉米科学, 2004, 12(增刊2): 35–37
Sun G W, Zhang F L, Guo J, Zhao J R, Guo J L. Discuss on the cause of maize kernel abortion. J Maize Sci, 2004, 12(suppl-2): 35–37 (in Chinese with English abstract)
[3]申丽霞, 王璞, 张红芳, 易镇邪. 施氮对夏玉米不同部位籽粒灌浆的影响. 作物学报, 2005, 31: 532−534
Shen L X, Wang P, Zhang H F, Yi Z X. Effect of nitrogen supply on grain filling at different ear position in summer maize. Acta Agron Sin, 2005, 31: 532–534 (in Chinese with English abstract)
[4]李玉玲, 台国琴. 玉米籽粒灌浆及种子萌发特性的粒位效应研究. 玉米科学, 2005, 13(1): 60–63
Li Y L, Tai G Q. Study on the kernel position effects of grain filling and seed germination characteristics in maize. J Maize Sci, 2005, 13(1): 60−63 (in Chinese with English abstract)
[5]Yang J C, Zhang J H, Wang Z Q, Zhu Q S. Hormones in the grains in relation to sink strength and postanthesis development of spikelets in rice. Plant Growth Regul, 2003, 41: 185−195
[6]Venkateswarlu B, Visperas R M. Source-sink relationships in crop plants. Int Rice Res Paper Ser, 1987, 125: 1−19
[7]Warren W J. Control of crop processes. In: Rees A R, Cockshull K E, Hand D W, Hurd R G, eds. Crop Processes in Controlled Environments. London/New York: Academic Press, 1972. pp 7−30
[8]Ho L C. Metabolism and compartmentation of imported sugars in sink organs in relation to sink strength. Annu Rev Plant Physiol Plant Mol Biol, 1988, 39: 355−378
[9]王纪华, 王树安, 赵冬梅, 倪军昌. 玉米籽粒发育的调控研究: III. 离体条件下的化学调控机理探讨. 作物学报, 1996, 22: 208−213
Wang J H, Wang S A, Zhao D M, Ni J C. Study on regulation of development of maize kernels: III. The approach to chemical regulation under conditions of tissue culture. Acta Agron Sin, 1996, 22: 208–213 (in Chinese with English abstract)
[10]唐祈林, 荣廷昭. 玉米秃尖与内源激素的关系. 核农学报, 2007, 21: 366–368
Tang Q L, Rong T Z. The relationship between endogenous hormone and barren ear tip of maize. J Nucl Agric Sci, 2007, 21: 366−368 (in Chinese with English abstract)
[11]张凤路, 赵久然, 王纪华. 离体条件下玉米籽粒发育调控研究. 杂粮作物, 2001, 21(4): 34–35
Zhang F L, Zhao J R, Wang J H. Regulating study on maize kernel development in vitro. Rain Fed Crops, 2001, 21(4): 34−35 (in Chinese with English abstract)
[12]Pan Y Q, Lou H L, Li Y R. Soluble acid invertase and sucrose phosphate synthase: key enzymes in regulating sucrose accumulation in sugarcane stalk. J Sugar Tech, 2009, 11(1): 28–33
[13]梁红伟, 高聚林, 王志刚, 于晓芳, 孙继颖, 胡树平, 王振, 余少波, 李雅剑. 基于控制授粉技术的玉米弱势粒发育与库特征的关系. 作物学报, 2016, 42: 927−933
Liang H W, Gao J L, Wang Z G, Yu X F, Sun J Y, Hu S P, Wang Z, Yu S B, Li Y J. Relationship of inferior kernel setting with sink strength under controlling pollination. Acta Agron Sin, 2016, 42: 927−933 (in Chinese with English abstract)
[14]徐云姬, 顾道健, 张博博, 张耗, 王志琴, 杨建昌. 玉米果穗不同部位籽粒激素含量及其与胚乳发育和籽粒灌浆的关系. 作物学报, 2013, 39: 1452–1461
Xu Y J, Gu D J, Zhang B B, Zhang H, Wang Z Q, Yang J C. Hormone contents in kernels at different positions on an ear and their relationship with endosperm development and kernel filling in maize. Acta Agron Sin, 2013, 39: 1452–1461 (in Chinese with English abstract)
[15]徐云姬, 顾道建, 秦昊, 张耗, 王志琴, 杨建昌. 玉米灌浆期果穗不同部位籽粒碳水化合物积累与淀粉合成相关酶活性变化. 作物学报, 2015, 41: 297–307
Xu Y J, Gu D J, Qing H, Zhang H, Wang Z Q, Yang J C. Changes in carbohydrate accumulation and activities of enzymes involved in starch synthesis in maize kernels at different positions on an ear during grain filling. Acta Agron Sin, 2015, 41: 297−307 (in Chinese with English abstract)
[16]Bangerth F. Dominance among fruits/sinks and the search for a correlative signal. Physiol Plant, 1989, 76: 608–614
[17]Morris R D, Blevins D G, Dietrich J T, Durley R C, Gelvin S B, Gray J, Hommes N G, Kaminek M, Mathews L J, Meilan R, Reinbott T M, Sayavedra-Soto L. Cytokinins in plant pathogenic bacteria and developing cereal grains. Aust J Plant Physiol, 1993, 20: 621–637
[18]Singh G, Gerung S B. Hormonal role in the problem of sterility in Oryza sativa. Plant Physiol Biochem, 1982, 9: 22–23
[19]孙庆泉, 吴元奇, 胡昌浩, 董树亭, 荣廷昭, 张颖. 不同产量潜力玉米子粒胚乳细胞增殖与子粒充实期的生理活性. 作物学报, 2005, 31: 612−618
Sun Q Q, Wu Y Q, Hu C H, Dong S T, Rong T Z, Zhang Y. Physiological activities and multiplication of endosperm cell at filling stage of kernels with different yield potential in maize. Acta Agron Sin, 2005, 31: 612−618 (in Chinese with English abstract)
[20]冯汉宇, 王志敏, 孔凡娜, 张敏洁, 周顺利. 基于控制授粉技术的玉米籽粒生育特性与建成机制研究. 作物学报, 2011, 37: 1605–1615
Feng H Y, Wang Z M, Kong F N, Zhang M J, Zhou S L. Mechanism of maize kernel setting and characteristics of kernel growth and development under controlled pollination. Acta Agron Sin, 2011, 37: 1605−1615 (in Chinese with English abstract)
[21]Feng H Y, Wang Z M, Kong F N, Zhang M J, Zhou S L. Roles of carbohydrate supply and ethylene, polyamines in maize kernel set. J Integr Plant Biol, 2011, 53: 388−398
[22]高俊凤. 植物生理学实验指导. 北京: 高等教育出版社, 2006. pp 105–108
Gao J F. Plant Physiology Experiment Instruction. Beijing: Higher Education Press, 2006. pp 105−108 (in Chinese)
[23]刘华英, 萧浪涛, 鲁旭东, 胡家金, 吴顺, 何长征, 邓秀新. 伏令夏橙愈伤组织体细胞胚发生中多胺水平的变化. 植物生理与分子生物学学报, 2005, 31: 275−280
Liu H Y , Xiao L T, Lu X D, Hu J J, Wu S, He C Z, Deng X X. Changes in polyamine levels in Citrus sinensis Osb. cv. valencia callus during somatic embryogenesis. J Plant Physiol Mol Biol, 2005, 31: 275−280 (in Chinese with English abstract)
[24]王艳芳, 李红, 张立军, 马兴林, 关义新. 春玉米籽粒灌浆期IAA对淀粉积累的作用及其机制的研究. 化学与生物工程, 2006, 23(3): 35–37
Wang Y F, Li H, Zhang L J, Ma X L, Guan Y X. Effect and mechanism of IAA to starch accumulation in grain during grain-filling stage in spring maize. Chem Bioeng, 2006, 23(3): 35–37 (in Chinese with English abstract)
[25]李连禄, 赵明, 丁在松. 玉米穗库发育的不同步性与内源激素作用的研究. 中国农业大学学报, 2001, 6(1): 30−36
Li L L, Zhao M, Ding Z S. Unsynchronism of the development of ear sinks and effects of endogenous hormones in maize. J China Agric Univ, 2001, 6(1): 30−36 (in Chinese with English abstract)
[26]覃章铮, 唐锡华. 高等植物胚胎的发育生物学研究: VI. 粳稻胚分化发育期间一些大分子物质的动态. 植物生理学报, 1982, 8: 295–305
Qin Z Z, Tang X H. Studies on the developmental biology of embryogenesis in higher plants: VI. Dynamic changes in some of the macromolecules during embryogenesis of Oryza sativa L. subsp. japonica. J Plant Physiol, 1982, 8: 295−305 (in Chinese with English abstract)
[27]Reed A J, Singletary G W. Roles of carbohydrate supply and phytohormones in maize kernel abortion. Plant Physiol, 1989, 91: 986−992
[28]杨建昌, 彭少兵, 顾世梁, Visperas R M, 朱庆森. 水稻灌浆期籽粒中3个与淀粉合成有关的酶活性变化. 作物学报, 2001, 27: 157–164
Yang J C, Peng S B, Gu S L, Visperas R M, Zhu Q S. Changes in activities of three enzymes associated with starch synthesis in rice grains during grain filling. Acta Agron Sin, 2001, 27: 157–164 (in Chinese with English abstract)
[29]Yang J C, Cao Y Y, Zhang H. Involvement of polyamines in the post-anthesis development of inferior and superior spikelets in rice. Planta, 2008, 228: 137−149
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