Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2023, Vol. 49 ›› Issue (11): 2876-2885.doi: 10.3724/SP.J.1006.2023.23066

;

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Application of maize 6H-60K chip in identification of maize essentially derived varieties

TIAN Hong-Li(), ZHANG Ru-Yang(), FAN Ya-Ming(), YANG Yang, ZHANG Yun-Long, YI Hong-Mei, XING Jin-Feng, WANG Feng-Ge(), ZHAO Jiu-Ran()   

  1. Maize Research Institute, Beijing Academy of Agriculture and Forestry Sciences / Key Laboratory of Crop DNA Fingerprinting Innovation and Utilization (Co-construction by Ministry and Province) / Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097, China
  • Received:2022-09-30 Accepted:2023-05-24 Online:2023-11-12 Published:2023-06-06
  • About author:First author contact:

    **Contributed equally to this study

  • Supported by:
    National Scientific and Technological Innovation—Major Projects(2022ZD04019);Science and Technology Innovation Capacity Building Project of BAAFS (KJCX20230301)(KJCX202303)

RichHTML416

44

PDF

623

Abstract:

The identification of maize essentially derived variety has become the hot spot in the intellectual property protection of seed industry. In order to speed up the establishment of its accurate and efficient molecular identification technology, this article used multiple types of derived varieties as research materials: Jing 2416 and Jing 2416C (two inbred lines with highly similar genetic backgrounds), Jing 724 and Jing 72464 (two inbred lines with similar genetic backgrounds), as well as 893 DH lines of genetic population constructed by Jing 724 and Jing 72464. The study researched and analyzed the potential of maize 6H-60K chip including 61,214 SNPs in identification of maize essentially derived varieties. The results were as follows: (1) There were 829 SNPs differences between Jing 2416 and Jing 2416C, the GS value (genetic similarity) was 98.7%, and 56.7% of the difference loci were concentrated in the 39 Mb region of chromosome 5. (2) There were 4912 SNPs differences between Jing 724 and Jing 72464, the GS value was 90.1%, and 44.8% of the difference loci were concentrated on chromosome 3. (3) The distribution of genetic similarity values between 893 DH lines and two parents (Jing 724 and Jing 72464) was continuous. The GS value range between 893 DH lines and Jing 724 was 88.0%-97.0%, with an average of 92.6%. The GS value range between 893 DH lines and Jing 72464 was 88.3%-98.6%, with an average of 94.5%. (4) 893 DH lines were paired comparison, a total of 398,278 pairs were compared. There were specific SNP differences between all DH lines. The GS values of 893 DH lines in pairs ranged from 87.5% to 99.9%, with an average of 94.3%. Among them, the proportion of GS value ≥ 97.0% was 8.6%, and the proportion of GS value ≥ 99.0% was 1.3%. This study showed that maize 6H-60K SNP sets could accurately evaluate the genetic background of maize derived, similar or extremely similar inbred and DH lines, identify and distinguish all materials one by one, and had the potential to further lock the linkage markers of derived traits. It is suggested that the technical system for maize essentially derived variety molecular identification based on Maize6H-60K SNP sets using chip, genotyping by target sequencing (GBTS) and other platforms should be urgently established, so as to provide technical support for intellectual property protection and variety innovation of maize varieties.

Key words: maize, the essentially derived variety, molecular identification, maize 6H-60K chip, the high-density SNP loci set

Fig. 1

Distribution of different loci between Jing 2416 and Jing 2416C in the whole genome"

Fig. 2

Distribution of different loci between Jing 724 and Jing 72464 in the whole genome (A and B), and recombinant frequency of 10 chromosomes based on DH lines groups (C)"

Fig. 3

Genetic similarity analysis between each DH line and Jing 724 and Jing 72464 The abscissa is the 893 DH lines, sorted from small to large according to the LS value with Jing 72464. The ordinate is the genetic similarity value."

Fig. 4

Distribution of genetic similarity between two DH lines of 893 DH lines The abscissa is the value of genetic similarity and locus similarity, and the ordinate is the number of paired analysis DH lines."

Fig. 5

Genetic segregation of DH line population constructed by Jing 724 and Jing 72464 Fig. A is the genetic segregation diagram of polymorphic markers, red is the partial maternal segregation loci, and the orange is the unbiased segregation loci. Figs. B, C, and D are genetic background of three DH lines (DH388, DH677, and DH543). Genotypes are indicated as follows: white area, the same homozygous genotype as Jing 724 and Jing 72464; green, the same homozygous genotype as Jing 724; red, the same homozygous genotype as Jing 72464."

[1] 褚云霞, 陈海荣, 邓姗, 黄志城, 李寿国. 实质性派生品种鉴定方法研究进展. 上海农业学报, 2017, 33(5): 132-138.
Chu Y X, Chen H R, Deng S, Huang Z C, Li S G. Development of the identification of essentially derived varieties. Acta Agric Shanghai, 2017, 33(5): 132-138 (in Chinese with English abstract).
[2] Heckenberger M, Bohn M, Ziegle J S, Joe L K, Hauser J D, Hutton M, Melchinger A E. Variation of DNA fingerprints among accessions within maize inbred lines and implications for identification of essentially derived varieties: I. Genetic and technical sources of variation in SSR data. Mol Breed, 2002, 10: 181-191.
doi: 10.1023/A:1020539330957
[3] Heckenberger M, Voort J R, Melchinger A E, Peleman J, Bohn M. Variation of DNA fingerprints among accessions within maize inbred lines and implications for identification of essentially derived varieties: II. Genetic and technical sources of variation in AFLP data and comparison with SSR data. Mol Breed, 2003, 12: 97-106.
doi: 10.1023/A:1026040007166
[4] Heckenberger M, Bohn M, Klein D, Melchinger A E. Identification of essentially derived varieties obtained from biparental crosses of homozygous lines: II. Morphological distances and heterosis in comparison with simple sequence repeat and amplified fragment length polymorphism data in maize. Crop Sci, 2005, 45: 1132-1140.
doi: 10.2135/cropsci2004.0111
[5] Heckenberger M, Muminović J, Voort J R, Peleman J, Bohn M, Melchinger A E. Identification of essentially derived varieties obtained from biparental crosses of homozygous lines: III. AFLP data from maize inbreds and comparison with SSR data. Mol Breed, 2006, 17: 111-125.
doi: 10.1007/s11032-005-3851-5
[6] Kahler A L, Kahler J L, Thompson S A, Ferriss R S, Jones E S, Nelson B K, Mikel M A, Smith S. North American study on essential derivation in maize: II. Selection and evaluation of a panel of simple sequence repeat loci. Crop Sci, 2010, 50: 486-503.
doi: 10.2135/cropsci2009.03.0121
[7] ISF. Guidelines for Handling Disputes on Essential Derivation of Maize Lines, 2008. www.worldseed.orgwww.worldseed.org.
[8] ISF. Guidelines for Handling Disputes on Essential Derivation of Maize Lines, 2014. www.worldseed.org.
[9] Rousselle Y, Jones E, Charcosset A, Moreau P, Robbins K, Stich B, Knaak C, Flament P, Karaman Z, Martinant J R, Fourneau M, Taillardat A, Romestant M, Tabel C, Bertran J, Ranc N, Lespinasse D, Blanchard P, Kahler A, Chen J, Kahler J, Dobrin S, Warner T, Ferris R, Smith S. Study on essential derivation in maize: III. Selection and evaluation of a panel of single nucleotide polymorphism loci for use in European and North American Germplasm. Crop Sci, 2015, 55:1170-1180.
doi: 10.2135/cropsci2014.09.0627
[10] 彭海, 方治伟, 李论, 马爱进, 周俊飞, 温常龙, 李甜甜, 唐浩, 陈红, 崔野韩, 张嘉楠, 贾英民, 许娜, 宋书锋, 胡美霞, 符习勤, 赵治海, 梁勇, 徐振江, 高利芬, 陈利红, 韩瑞玺, 张蝶, 张静, 余进文. 植物品种鉴定MNP标记法. 中华人民共和国国家标准, GB/T 38551-2020, 2020.
Peng H, Fang Z W, Li L, Ma A J, Zhou J F, Wen C L, Li T T, Tang H, Chen H, Cui Y H, Zhang J N, Jia Y M, Xu N, Song S F, Hu M X, Fu X Q, Zhao Z H, Liang Y, Xu Z J, Gao L F, Chen L H, Han R X, Zhang D, Zhang J, Yu J W. Identification of plant varieties: MNP marker method. National Standards of the People’s Republic of China, GB/T 38551-2020 2020, (in Chinese).
[11] UPOV International Union for the Protection of New Varieties of Plants. Possible Used of Molecular Markers in the Examination of Distinctness, Uniformity and Stability (DUS). Geneva, Switzerland: UPOV, 2011.
[12] 徐云碧, 王冰冰, 张健, 张嘉楠, 李建生. 应用分子标记技术改进作物品种保护和监管. 作物学报, 2022, 48: 1853-1870.
doi: 10.3724/SP.J.1006.2022.23001
Xu Y B, Wang B B, Zhang J, Zhang J N, Li J S. Enhancement of plant variety protection and regulation using molecular marker technology. Acta Agron Sin, 2022, 48: 1853-1870 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2022.23001
[13] Ganal M W, Durstewitz G, Polley A, Bérard A, Buckler E S, Charcosset A, Clarke J D, Graner E M, Hansen M, Joets J, Paslier M C L, McMullen M D, Montalent P, Rose M, Schön C C, Sun Q, Walter H, Martin O C, Falque M. A large maize (Zea mays L.) SNP genotyping array: development and germplasm genotyping, and genetic mapping to compare with the B73 reference genome. PLoS One, 2011, 6: e28334.
doi: 10.1371/journal.pone.0028334
[14] Unterseer S, Bauer E, Haberer G, Seidel M, Knaak C, Ouzunova M, Meitinger T, Strom T M, Fries R, Pausch H, Bertani C, Davassi A, Mayer K F, Schön C C. A powerful tool for genome analysis in maize: development and evaluation of the high density 600k SNP genotyping array. BMC Genomics, 2014, 15: 823.
doi: 10.1186/1471-2164-15-823
[15] Xu C, Ren Y H, Jian Y Q, Guo Z F, Zhang Y, Xie C X, Fu J J, Wang H W, Wang G Y, Xu Y B, Li P, Zou C. Development of a maize 55K SNP array with improved genome coverage for molecular breeding. Mol Breed, 2017, 37: 20.
doi: 10.1007/s11032-017-0622-z
[16] Tian H L, Yang Y, Yi H M, Xu L W, He H, Fan Y M, Wang L, Ge J R, Liu Y W, Wang F G, Zhao J R. New resources for genetic studies in maize (Zea mays L.): a genome-wide Maize6H-60K single nucleotide polymorphism array and its application. Plant J, 2021, 105: 1113-1122.
doi: 10.1111/tpj.v105.4
[17] 王凤格, 晋芳, 田红丽, 易红梅, 赵久然, 金石桥, 杨扬, 王蕊, 葛建镕, 支巨振, 赵建宗. 玉米品种真实性鉴定SNP标记法. 中华人民共和国农业行业标准, NY/T 4022-2021, 2021.
Wang F G, Jin F, Tian H L, Yi H M, Zhao J R, Jin S Q, Yang Y, Wang R, Ge J R, Zhi J Z, Zhao J Z. Maize (Zea mays L.) variety genuineness identification: SNP based method. Agricultural Industry Standards of the People’s Republic of China, NY/T 4022-2021 2021 (in Chinese).
[18] 赵久然, 王元东, 宋伟, 张如养, 李春辉, 刘新香. 玉米骨干自交系京2416的选育与应用. 植物遗传资源学报, 2020, 21: 1051-1057.
doi: 10.13430/j.cnki.jpgr.20200221001
Zhao J R, Wang Y D, Song W, Zhang R Y, Li C H, Liu X X. Breeding and application of maize founder inbred line Jing 2416. J Plant Genet Resour, 2020, 21: 1051-1057 (in Chinese with English abstract).
[19] 赵久然, 李春辉, 宋伟, 刘新香, 王元东, 张如养, 王继东, 孙轩, 王夏青. 玉米骨干自交系京2416杂种优势及遗传重组解析. 中国农业科学, 2020, 53: 4527-4536.
doi: 10.3864/j.issn.0578-1752.2020.22.001
Zhao J R, Li C H, Song W, Liu X X, Wang Y D, Zhang R Y, Wang J D, Sun X, Wang X Q. Heterosis and genetic recombination dissection of maize key inbred line Jing 2416. Sci Agric Sin, 2020, 53: 4527-4536 (in Chinese with English abstract).
[20] 王凤格, 易红梅, 赵久然, 刘平, 张新明, 田红丽, 堵苑苑. 玉米品种鉴定技术规程SSR标记法. 中华人民共和国农业行业标准, NY/T 1432-2014, 2014.
Wang F G, Yi H M, Zhao J R, Liu P, Zhang X M, Tian H L, Du Y Y. Protocol for the Identification of Maize Varieties: SSR Marker Method. Agricultural Industry Standards of the People’s Republic of China, NY/T 1432-2014 2014 (in Chinese).
[21] 赵久然, 李春辉, 宋伟, 王元东, 张如养, 王继东, 王凤格, 田红丽, 王蕊. 基于SNP芯片揭示中国玉米育种种质的遗传多样性与群体遗传结构. 中国农业科学, 2018, 51: 626-634.
doi: 10.3864/j.issn.0578-1752.2018.04.003
Zhao J R, Li C H, Song W, Wang Y D, Zhang R Y, Wang J D, Wang F G, Tian H L, Wang R. Genetic diversity and population structure of important Chinese maize breeding germplasm revealed by SNP-Chips. Sci Agric Sin, 2018, 51: 626-634 (in Chinese with English abstract).
doi: 10.3864/j.issn.0578-1752.2018.04.003
[22] Oróstica K Y, Verdugo R A. ChromPlot: visualization of genomic data in chromosomal context. Bioinformatics, 2016, 32: 2366-2369.
doi: 10.1093/bioinformatics/btw137 pmid: 27153580
[23] 赵久然, 王凤格, 田红丽, 易红梅, 王蕊, 葛建镕. 适于农作物品种分子身份鉴别和确权鉴定的检测方法. 中国发明专利, 2017, ZL 201710527354.5.
Zhao J R, Wang F G, Tian H L, Yi H M, Wang R, Ge J R. Detection Methods for Identity Distinguish and Intellectual Property Confirmation of Crop Varieties Using Molecular Markers. Chinese Invention Patent, 2017, ZL 201710527354.5. (in Chinese).
[24] Zhao Y K, Tian H L, Li C H, Yi H M, Zhang Y L, Li X H, Zhao H, Huo Y X, Wang R, Kang D M, Lu Y C, Liu Z H, Liang Z Y, Xu L W, Yang Y, Zhou L, Wang T Y, Zhao J R, Wang F G. HTPdb and HTPtools: exploiting maize haplotype-tag polymorphisms for germplasm resource analyses and genomics-informed breeding. Plant Commun, 2022, 3: 100331.
doi: 10.1016/j.xplc.2022.100331
[25] 徐云碧, 杨泉女, 郑洪建, 许彦芬, 桑志勤, 郭子锋, 彭海, 张丛, 蓝昊发, 王蕴波, 吴坤生, 陶家军, 张嘉楠. 靶向测序基因型检测(GBTS)技术及其应用. 中国农业科学, 2020, 53: 2983-3004.
doi: 10.3864/j.issn.0578-1752.2020.15.001
Xu Y, Yang Q N, Zheng H J, Xu Y F, Sang Z Q, Guo Z F, Peng H, Zhang C, Lan H F, Wang Y B, Wu K S, Tao J J, Zhang J N. Genotyping by target sequencing (GBTS) and its applications. Sci Agric Sin, 2020, 53: 2983-3004.
doi: 10.3864/j.issn.0578-1752.2020.15.001
[26] 崔野韩, 温雯, 陈红, 杨扬, 堵苑苑, 卢新. 我国农业植物新品种保护工作回顾与展望. 中国种业, 2019, (2): 9-11.
Cui Y H, Wen W, Chen H, Yang Y, Du Y Y, Lu X. Review and respect of the plant new variety protection in China. China Seed Industry, 2019, (2): 9-11 (in Chinese).
[27] 邓伟, 崔野韩. 中国农业植物新品种保护制度及发展的研究. 中国种业, 2020, (11): 1-7.
Deng W, Cui Y H. Study on the protection system and development of the plant new variety protection in China. China Seed Industry, 2020, (11): 1-7 (in Chinese).
[28] 温雯, 闫东哲, 刘衎, 崔野韩. 健全我国农业植物新品种保护制度体系的思考. 农业科技管理, 2022, 41(1): 71-75.
Wen W, Yan D Z, Liu K, Cui Y H. Considerations on perfecting agricultural protection system of new plant varieties in China. Manag Agric Sci Technol, 2022, 41(1): 71-75 (in Chinese with English abstract).
[29] Smith J S C. The future of essentially derived variety (EDV) status: predominantly more explanations or essential change. Agronomy, 2021, 11: 1261.
doi: 10.3390/agronomy11061261
[30] 万志前, 张媛. 实质性派生品种制度的缘起、困境与困应. 浙江农业学报, 2020, 32: 2067-2076.
doi: 10.3969/j.issn.1004-1524.2020.11.18
Wan Z Q, Zhang Y. Origin, implementation difficulties and countermeasures of essential derived variety system. Acta Agric Zhejiangensis, 2020, 32: 2067-2076 (in Chinese with English abstract).
doi: 10.3969/j.issn.1004-1524.2020.11.18
[31] 张上都, 袁定阳, 路洪凤, 简燕, 李秀欣, 黄安平, 罗正良, 吕启明, 谭炎宁, 张勇飞, 袁隆平, 柏连阳. 基因组学方法用于水稻种质资源实质派生的检测结果和应用讨论. 中国科学: 生命科学, 2020, 50: 633-649.
Zhang S D, Yuan D Y, Lu H F, Jian Y, Li X X, Huang A P, Luo Z L, Lyu Q M, Tan Y N, Zhang Y F, Yuan L P, Bai L Y. The results of rice germplasm EDV test by genomic analysis and related discussions. Sci Sin: Life Sci, 2020, 50: 633-649 (in Chinese with English abstract).
[32] 简燕, 李小波, 王博, 赵静, 索海翠, 黄安平, 胡柏耿, 曹春梅, 张勇飞. 马铃薯实质派生品种鉴定的基因组学技术. 中国马铃薯, 2020, 34: 321-328.
Jian Y, Li X B, Wang B, Zhao J, Suo H C, Huang A P, Hu B G, Cao C M, Zhang Y F. Genomics technique for detection of potato essentially derived variety. Chin Potato J, 2020, 34: 321-328 (in Chinese with English abstract).
[33] 田红丽, 杨扬, 王璐, 王蕊, 易红梅, 许理文, 张云龙, 葛建镕, 王凤格, 赵久然. 兼容型maizeSNP384标记筛选与玉米杂交种DNA指纹图谱构建. 作物学报, 2020, 46: 1006-1015.
doi: 10.3724/SP.J.1006.2020.93048
Tian H L, Yang Y, Wang L, Wang R, Yi H M, Xu L W, Zhang Y L, Ge J R, Wang F G, Zhao J R. Screening of compatible maizeSNP384 markers and the construction of DNA fingerprints of maize varieties. Acta Agron Sin, 2020, 46: 1006-1015 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2020.93048
[34] Noli E, Teriaca M S, Conti S. Criteria for the definition of similarity thresholds for identifying essentially derived varieties. Plant Breed, 2013, 132: 525-531.
doi: 10.1111/pbr.12109
[1] Liang Jin-Yu, Yin Jia-De, Wang Hong-Li, Zhang Guo-Ping, Hou Hui-Zhi, Dong Bo, Ma Ming-Sheng. Estimation of leaf nitrogen content in dryland forage maize using UAV-based hyperspectral imaging and machine learning [J]. Acta Agronomica Sinica, 2026, 52(6): 1788-1801.
[2] Yang Xin-Yu, Cui Wen-Tao, Dilinigeer Alimu, Wang Kai-Xiang, Wu Peng-Hao, Ren Jiao-Jiao. Genome-wide association and genomic selection analysis of the number of leaves above the ear in maize [J]. Acta Agronomica Sinica, 2026, 52(5): 1573-1590.
[3] Han Ya-Xin, He Guan-Hua, Zhang Xiao-Qiong, Zhang Deng-Feng, Li Yong-Xiang, Liu Xu-Yang, Wang Tian-Yu, Li Yu, Zou Hua-Wen, Li Chun-Hui. Identification of maize lateral root density genes resources through integrated RNA-seq and BSA-seq analyses [J]. Acta Agronomica Sinica, 2026, 52(5): 1341-1352.
[4] Sun Shu-Feng, Xu Zhen-Nan, Huang Jia-Xin, Weng Jian-Feng, Li Xin-Hai. Genome-wide identification of the maize MAPK gene family and its response to Fusarium verticillioides infection [J]. Acta Agronomica Sinica, 2026, 52(5): 1291-1308.
[5] Zhang Ning-Ning, Teng Yu-Fei, Ren Na-Na, Wei Xing-Zhuo, Yan Shu-Hao, Fan Ke-Xin, Wang Yong-Hong, Chen Wen-Kang, Zhang Xing-Hua, Zhu Wan-Chao, Xu Shu-Tu, Xue Ji-Quan. Phenotypic evaluation and plasticity analysis of drought resistance in 201 maize inbred lines [J]. Acta Agronomica Sinica, 2026, 52(5): 1309-1325.
[6] Zhang Hong-Rong, Wang Fei-Er, Li Pan, Qiu Hai-Long, Zhu Jing, Zhao Lian-Hao, Nan Yun-You, He Wei, Fan Zhi-Long, Hu Fa-Long, Chai Qiang, Yin Wen. Photosynthetic characteristics of 20% reduced irrigation combined with 25% organic substitution for chemical fertilizer in increasing silage maize yield [J]. Acta Agronomica Sinica, 2026, 52(5): 1487-1500.
[7] Yang Yang, Chang Shi-Hui, Tian Hong-Li, Yi Hong-Mei, Wang Lu, Ren Jie, Fan Ya-Ming, Liu Ya-Wei, Wang Feng-Ge, Zhao Jiu-Ran. Genetic diversity analysis of nationally approved maize varieties in different ecological regions [J]. Acta Agronomica Sinica, 2026, 52(5): 1352-1364.
[8] Cai Hong-Wei, Yu Ai-Zhong, Jiang Ke-Qiang, Wang Peng-Fei, Wang Yu-Long, Huo Jian-Zhe, Pang Xiao-Neng, Yin Bo, Shang Yong-Pan. Key mechanisms underlying the enhancement of sweet maize yield through partial substitution of chemical fertilizers with organic manure in arid irrigation districts [J]. Acta Agronomica Sinica, 2026, 52(4): 1166-1180.
[9] Tian Hong-Li, Yang Yang, Fan Ya-Ming, Yi Hong-Mei, Guo Dan-Dan, Wang Feng-Ge, Zhao Jiu-Ran. A novel set of tri-allelic variant SNP loci suitable for maize variety identification [J]. Acta Agronomica Sinica, 2026, 52(4): 993-1005.
[10] Guo Xiang-Yang, Tu Liang, Wang Dong, Liu Peng-Fei, Wang An-Gui, Yi Qiang, Ren Hong, Li Gang, Zhu Yun-Fang, Wu Xun, Jiang Yu-Lin, Tian Feng, Chen Ze-Hui. Application and prospects of Suwan germplasm in maize breeding in China [J]. Acta Agronomica Sinica, 2026, 52(3): 655-664.
[11] Ma Liang, Ma Lu, Zhang Shu-Yu, Zhang Hui-Min, Wang Ren-Ming, Song Xu-Dong, Zhang Zhen-Liang, Mao Yu-Xiang, Lu Hu-Hua, Chen Guo-Qing, Hao De-Rong, Zhou Guang-Fei. Transcriptome analysis and identification of candidate genes associated with husk number in maize [J]. Acta Agronomica Sinica, 2026, 52(3): 790-801.
[12] Meng Cheng, Wang Zhe. Genome-wide identification and expression analysis of the ZmPFK gene family under biotic and abiotic stresses in maize [J]. Acta Agronomica Sinica, 2026, 52(3): 764-779.
[13] Li Xin-Hao, Xing Meng-Ke, Zhou Zi-Hui, Li Si-Ye, Ren Hao, Wang Hong-Zhang, Lai Hua-Jiang. Exogenous melatonin enhances heat tolerance of maize at the seedling stage by coordinating light and dark reactions [J]. Acta Agronomica Sinica, 2026, 52(3): 839-856.
[14] Liu Ji-Chang, Li Si-Ye, Li Xue-Ting, Wang Hong-Zhang, Liu Peng, Zhang Ji-Wang, Zhao Bin, Ren Bai-Zhao, Ren Hao. Effects of salt stress on root growth and nutrient absorption efficiency of different salt-tolerant summer maize varieties [J]. Acta Agronomica Sinica, 2026, 52(2): 565-577.
[15] Lin Zi-Qing, Zhong Xing-Yu, Liu Fan, Ren Zi-Ao, Ma Rui, Deng Xiu-Feng, Wang Dong-Wei, Liu Shao-Peng, Chen Kang, Zhang Ming-Cai, Li Zhao-Hu, Zhou Yu-Yi, Duan Liu-Sheng. Development of ultra-high-yield technology for a wheat-maize double cropping system achieving a 2-ton annual grain yield per mu in the coastal plain of Northern Shandong peninsula, China [J]. Acta Agronomica Sinica, 2026, 52(2): 631-643.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd