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Table of Content

    12 June 2026, Volume 52 Issue 6
    • CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS
      Analysis of the genetic effects of allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci on peroxidase activity in wheat grains
      Zhai Sheng-Nan, Cao Xin-You, Li Hao-Sheng, Li Ji-Hu, Li Fa-Ji, Liu Jin-Dong, Xia Xian-Chun, Lyu Ying-Ying, Ma Rui-Feng, Wang Ying, Geng Hong-Wei, Liu Jian-Jun
      Acta Agronomica Sinica. 2026, 52(6):  1593-1603.  doi:10.3724/SP.J.1006.2026.51106
      Abstract ( 492 )   HTML ( 38 )   PDF (579KB) ( 182 )   Save
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      Peroxidase (POD) activity in wheat grains strongly influences processing quality and the color of wheat-based products, and is therefore an important target for quality improvement. In this study, 151 wheat varieties (lines) from domestic and international sources were used to systematically characterize allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci using molecular markers. Together with multi-year, multi-location measurements of grain POD activity, we evaluated the effects of individual alleles and their combinations on POD activity. Grain POD activity showed substantial genetic variation, with a mean of 674.39 U g-1 min-1, a range of 431.30-954.81 U g-1 min-1, and a coefficient of variation of 15.52%. Environment, genotype, and their interaction all had highly significant effects on POD activity (P < 0.01). All three loci significantly affected grain POD activity, and the favorable alleles were Pod-A1b, Pod-D1b, and Pod-2D-GG, respectively. In total, 12 allelic combinations were identified and classified into high-, medium-, and low-activity combination types based on grain POD activity. POD activity differed significantly among the three types (P < 0.05), whereas no significant differences were detected within each type. The genetic effects of the three loci on grain POD activity followed the order Pod-2D > Pod-A1 > Pod-D1. Grain POD activity increased significantly with the number of favorable alleles (R2 = 0.9681, P < 0.05); varieties (lines) pyramiding 2-3 favorable alleles had significantly higher POD activity than those carrying 0-1 favorable allele (P < 0.05). Significant regional differences were observed in POD activity, allelic variation, and allelic-combination frequencies. Nine varieties, including Liangxing 66, Lankao 24, and Jimai 22, were identified as carrying all three favorable alleles and exhibiting POD activity above 800 U g-1 min-1. These findings provide a theoretical basis and germplasm resources for molecular marker-assisted breeding to optimize wheat grain POD activity.

      Genome-wide association study and candidate gene prediction of grain copper content in wheat
      Xi Qian-Hui, Xu Zi-Yuan, Liu Meng-Meng, Wang Hong-Yi, Lang Kai-Lin, Jing Zhen-Hai, Chen Feng, Zhao Lei
      Acta Agronomica Sinica. 2026, 52(6):  1604-1617.  doi:10.3724/SP.J.1006.2026.51092
      Abstract ( 537 )   HTML ( 26 )   PDF (9859KB) ( 244 )   Save
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      Copper is an essential trace element in humans, and copper deficiency can compromise health. Wheat is a major staple crop worldwide, and biofortification of wheat grain with copper is a cost-effective strategy to help alleviate dietary copper deficiency. However, the genetic basis of grain copper content in wheat remains poorly understood. Here grain copper content was evaluated in 349 wheat accessions, and a genome-wide association study (GWAS) was performed using the Wheat 660K SNP array. In total, 775 significant SNPs were identified, mainly on chromosomes 1B, 4A, and 7A; among these, 56 SNPs were consistently detected in four or more replicates. Haplotype analysis indicated that GCuC_Hap_1B and GCuC_Hap_4A are key loci associated with grain copper content and show a significant pyramiding effect. Based on bioinformatics and haplotype analysis, TraesCS1B03G1265400 and TraesCS4A03G0093900 were proposed as candidate genes for regulating grain copper content. These results clarify the genetic architecture of grain copper content in wheat and provide a valuable foundation for developing germplasm with elevated grain copper content for wheat quality improvement breeding.

      Interaction between the sugarcane tetraspanin-like protein ScTSPAN18 and 6K2 in response to SCMV infection
      Cui Zhi-Yuan, Qin Chen-Zhan, Liu Xing-Yu, Zhang Hai, Zeng Kang, Huang Guo-Qiang, Xu Jing-Sheng
      Acta Agronomica Sinica. 2026, 52(6):  1618-1630.  doi:10.3724/SP.J.1006.2026.54151
      Abstract ( 405 )   HTML ( 7 )   PDF (11994KB) ( 133 )   Save
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      Tetraspanins (TETs) are a conserved class of transmembrane proteins that are widely distributed across cellular organisms and play important roles. TETs function in part by assembling tetraspanin-enriched microdomains (TEMs). In our previous study, we screened and identified a TET-like protein, ScTSPAN18, from a sugarcane cDNA yeast library using the 6K2 protein of sugarcane mosaic virus (SCMV) as bait, and we also characterized the TET gene family in the sugarcane (Saccharum spp. hybrid) cultivar Xintaitang 22 (XTT22). Here, the interaction between SCMV-6K2 and ScTSPAN18 was further validated using bimolecular fluorescence complementation (BiFC) and luciferase complementation assay (LCA). Bioinformatic analyses showed that ScTSPAN18 is 251 amino acids long and comprises four transmembrane domains. Structural modeling indicated that ScTSPAN18 differs from classical TETs, and yeast two-hybrid (Y2H) assays showed that ScTSPAN18 neither self-interacts nor interacts with classical TETs. Phylogenetic analysis suggested that TET like proteins are divergent between monocotyledonous and dicotyledonous species, as well as between C3 and C4 plants. Subcellular localization assays indicated that ScTSPAN18 is localized to the endoplasmic reticulum. RT-qPCR analysis showed that ScTSPAN18 exhibits tissue-specific expression, with relatively high transcript levels in leaves and roots; expression in the +1 leaf was significantly higher than that in the 3rd and 8th internodes. Following SCMV challenge, ScTSPAN18 expression increased significantly at early infection stages and remained high until day 14, after which it decreased to the basal level.

      Screening of low-light-tolerant potato varieties and cloning and functional analysis of the transcription factor gene StPIF3
      Wang Wen-Yuan, Yan Xue-Jia, Liu Yu-Lin, Sun Xiao-Tong, Li Ya-Nan, Tang Xin-Hua, Shi Ying
      Acta Agronomica Sinica. 2026, 52(6):  1631-1645.  doi:10.3724/SP.J.1006.2026.54135
      Abstract ( 415 )   HTML ( 9 )   PDF (4286KB) ( 167 )   Save
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      Based on measurements and comparisons of growth traits, chlorophyll fluorescence parameters, and other related indices in nine potato varieties under low-light treatment, the cultivar “Atlantic” was identified as low-light tolerant by principal component analysis. To investigate genes involved in the low-light response of “Atlantic”, we performed transcriptome analysis after treatment with a light intensity of 10 μmol m-2 s-1 for 48 h. Using GO enrichment, KEGG pathway analysis, and transcription factor prediction, we identified the light-responsive transcription factor gene StPIF3 and cloned its full-length cDNA. StPIF3 was introduced into tobacco via agrobacterium-mediated transformation, and T1 transgenic lines were obtained by selfing. Bioinformatics analysis, RT-qPCR, and chlorophyll fluorescence measurements were used to characterize and validate its function. StPIF3 encodes a 716-amino-acid protein with a predicted molecular weight of 76.77 kD and an isoelectric point of 7.26, and it is predicted to be hydrophilic and unstable. The StPIF3 protein contains a typical HLH domain and belongs to the bHLH family; phylogenetic analysis indicated that StPIF3 is most closely related to the tobacco homolog. Under 50 μmol m-2 s-1 light intensity, transgenic tobacco plants showed significantly higher Fv/Fm, qP, ETR, ETRmax, and SPAD values than wild-type plants, whereas F0 was lower. Overall, overexpression of StPIF3 markedly improves electron transport and light energy use efficiency, thereby enhancing photosynthetic performance and low-light tolerance.

      QTL mapping of total very long-chain fatty acids and seven fatty acid components in peanut seeds
      Zheng Yu-Zhen, Qi Fei-Yan, Sun Zi-Qi, Liu Hua, Qin Li, Shi Lei, Wang Juan, Wang Meng-Meng, Han Suo-Yi, Xu Jing, Miao Li-Juan, Huang Bing-Yan, Dong Wen-Zhao, Zheng Zheng, Zhang Xin-You
      Acta Agronomica Sinica. 2026, 52(6):  1646-1657.  doi:10.3724/SP.J.1006.2026.55071
      Abstract ( 530 )   HTML ( 5 )   PDF (1103KB) ( 86 )   Save
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      Peanut oil quality is largely determined by its fatty acid composition; therefore, improving the fatty acid profile is a key objective in peanut breeding. Here, we used a recombinant inbred line (RIL) population derived from the cross Jihuatian 1 × PI478819 to map quantitative trait loci (QTLs) for total very long-chain fatty acids (TVLCFAs) and seven individual fatty acid components across two environments, using a combined strategy of BSA-seq and conventional linkage mapping. Based on the major QTLs, we developed kompetitive allele-specific PCR (KASP) markers and validated them in the RIL population. In total, seven QTLs associated with TVLCFAs and the seven fatty acid components were identified. Three QTLs (qA06.1, qA08.1, and qA16.1) were consistently detected in both environments and co-localized for TVLCFAs and behenic acid content, explaining 7.10%-32.85% of the phenotypic variance (PVE). Notably, qA08.1 was also associated with oleic acid, linoleic acid, and palmitic acid contents in both environments, with PVE values of 13.73%-29.33%. The markers Tif2.A06.115805462, Tif2.A08.45741511, and Tif2.A16.148167815, developed for these three major QTLs, effectively distinguished lines with contrasting levels of TVLCFAs, arachidic acid, and behenic acid. In addition, Tif2.A08.45741511 was closely associated with oleic acid, linoleic acid, and palmitic acid contents. Overall, these results provide useful genetic resources for breeding peanuts with low TVLCFAs and high oleic acid content and support accelerated marker-assisted selection for high-quality specialty peanut cultivars.

      Positively regulating role of the key evening complex gene AhLUX1 in peanut nodulation
      Lu Yi-Chu, Li Zhen-Ying, Mai Chun-Hai, Zhao Xiao-Rui, Wang Li-Xiang
      Acta Agronomica Sinica. 2026, 52(6):  1658-1668.  doi:10.3724/SP.J.1006.2026.55069
      Abstract ( 328 )   HTML ( 20 )   PDF (11255KB) ( 104 )   Save
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      Biological nitrogen fixation via nodulation in legumes is highly energy intensive. In legumes, light signals not only regulate photosynthesis, morphogenesis, and growth, but also play key roles in controlling nodulation and nitrogen fixation during interactions with rhizobia. LUX (lux arrhythmo) is a core component of the evening complex (EC), which ensures accurate temporal output of multiple signals under light-dark cycles. Unlike soybean and many other legumes, peanut primarily uses a crack-entry mode of rhizobial invasion, and the role of AhLUX1 in peanut nodulation has remained unclear. Here, this study shows that AhLUX1 is strongly induced by rhizobial infection and is predominantly expressed in root tips, lateral root primordia, developing nodules, and mature nodules. Subcellular localization analysis indicates that AhLUX1 is localized in the nucleus. Functional analyses show that overexpression of AhLUX1 (OE-AhLUX1) significantly increases nodule number, whereas silencing (RNAi-AhLUX1) or knockout (CR-AhLUX1) markedly reduces nodulation, demonstrating that AhLUX1 is a positive regulator of nodulation in peanut. To clarify the underlying mechanism, the expression of nodulation marker genes in AhLUX1-overexpressing and AhLUX1-silenced roots. The results suggest that AhLUX1 modulates peanut nodulation by regulating the expression of key nodulation genes. Together, these findings link a light-signaling component to belowground nodulation and nitrogen fixation and provide a genetic resource for breeding high-efficiency peanut varieties through manipulation of the light-signaling pathway.

      Evaluation of powdery mildew resistance in wheat cultivars and molecular detection of resistance genes in Shanxi province, China
      Mao Jia-Qi, Huang Peng-Yu, Zhao Jia-Jia, Zheng Xing-Wei, Wu Bang-Bang, Hao Yu-Qiong, Qu Fei, Liu Cheng, Ma Peng-Tao, Zheng Jun
      Acta Agronomica Sinica. 2026, 52(6):  1669-1681.  doi:10.3724/SP.J.1006.2026.51095
      Abstract ( 423 )   HTML ( 29 )   PDF (5978KB) ( 157 )   Save
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      Powdery mildew (PM) is one of the major diseases threatening wheat production worldwide. Accurate identification of powdery mildew resistance in wheat cultivars and dissection of its genetic basis are crucial for breeding and deploying disease-resistant cultivars. In this study, we evaluated seedling- and adult-plant resistance to powdery mildew in 326 wheat accessions. We then used 11 molecular markers targeting Pm genes that are commonly used in breeding or confer high levels of resistance, including Pm2b, Pm4, Pm8, and Pm21, to detect resistance alleles. To identify additional Pm loci, we also performed a genome-wide association study (GWAS) using a wheat 16K SNP array. The results showed that 10 cultivars (e.g., Jinfeng 3, Shengmai 104, and Linmai 5311) were resistant to the Bgt isolate E09, indicating an overall lack of seedling resistance among the evaluated accessions. At the adult stage, 29 cultivars (e.g., Yunhei 28, Yaomai 30, and Yunhei 14207) were resistant to mixed Bgt isolates, and only four cultivars (Shengmai 104, JT176, Linmai 5311, and Jinfeng 3) displayed good resistance at both the seedling and adult stages. Multi-isolate tests of seedling resistance further indicated that Jinfeng 3, Shengmai 104, and JT176 were highly resistant to most isolates evaluated, representing valuable germplasm for powdery mildew resistance breeding. Marker-based analysis suggested that Shanxi wheat has a narrow genetic basis for powdery mildew resistance and tends to carry only one major Pm gene. Of the 11 Pm genes assayed, seven genes (e.g., Pm2b, Pm5e, and Pm6) were detected, whereas Pm1a, Pm24, Pm60, and Pm69 were not detected. In total, 108 accessions carried only one of the tested Pm genes; Pm8 was the most frequent, occurring in 14.11% of accessions. For gene pyramiding, the most common combinations were Pm5e+Pm8 and Pm6+Pm8, and only one cultivar carried four Pm genes. GWAS identified 15 stable loci across eight chromosomes; haplotype-phenotype analyses revealed significant associations at loci on chromosomes 1A, 2D, 4B, and 6A, and loci on 2D, 4B, 7B, and 7D may harbor novel powdery mildew resistance loci.

      Identification of class I LBD family members in upland cotton and function and haplotype analyses of GhLBD6 in regulating flowering period
      Peng Jia-Luo, Li Ying, Li Dan-Dan, Yang Jun-Ning, Guo Xue-Feng, Zhang Wen-Jiao, Yu Xiao-Xue, Zhou Ya-Rong, Wang Zhen-Yu, Wang Cai-Xiang, Ma Xiong-Feng, Su Jun-Ji
      Acta Agronomica Sinica. 2026, 52(6):  1682-1697.  doi:10.3724/SP.J.1006.2026.54138
      Abstract ( 519 )   HTML ( 12 )   PDF (7631KB) ( 186 )   Save
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      Lateral organ boundaries domain (LBD) family genes play important roles in a wide range of plant biological processes. Previous studies have shown that class I LBD genes are involved in the regulation of flowering time; however, whether class I LBD genes have similar functions in upland cotton remains unclear. In this study, we identified and characterized class I LBD family members at the whole-genome level in upland cotton and screened flowering-related candidate genes through haplotype analysis. The expression patterns of candidate genes in different tissues and in early- and late-flowering varieties were examined using RNA-seq and RT-qPCR, respectively. The function of the target gene was validated by virus-induced gene silencing (VIGS), followed by analysis of its breeding-related evolutionary dynamics and the development of molecular markers. In total, 102 class I GhLBD genes were identified; they were unevenly distributed across 26 chromosomes and were classified into six groups based on evolutionary relationships. LBD members within the same group showed similar motif composition and arrangement. Integrating haplotype and expression analyses, GhLBD6 was identified as a candidate gene associated with flowering time. VIGS combined with paraffin sectioning and RT-qPCR showed that suppression of GhLBD6 accelerated floral bud differentiation and significantly advanced budding and flowering by 6.57 d and 6.86 d, respectively. Two haplotypes (GhLBD6-Hap 1 and GhLBD6-Hap 2) were identified in the GhLBD6 coding region; among them, GhLBD6-Hap 1 represents a favorable early-flowering allele and appears to have been under artificial selection during upland cotton breeding. Finally, we developed a kompetitive allele-specific PCR (KASP) molecular marker to distinguish the two GhLBD6 haplotypes. Together, these forward- and reverse-genetic results demonstrate that GhLBD6 contributes to flowering-time regulation in upland cotton, providing a basis for molecular breeding of early-maturing cotton.

      Molecular cloning and expression analysis of BoPUB3L associated with self-incompatibility in Brasscia oleracea
      Zuo Tong-Hong, Zhang He-Cui, Zeng Jing, Zhu Li-Quan
      Acta Agronomica Sinica. 2026, 52(6):  1698-1710.  doi:10.3724/SP.J.1006.2026.54133
      Abstract ( 411 )   HTML ( 20 )   PDF (10440KB) ( 113 )   Save
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      U-box E3 ligases (PUBs) regulate plant self-incompatibility (SI), hormone signaling, and stress responses. By mining time-course transcriptome data from Brassica oleracea following self-pollination (0-60 min), we identified BoPUB3L, a PUB gene that is rapidly and specifically upregulated by self-pollination. The 2214 bp open reading frame encodes a 737-aa protein (81.27 kD; pI 6.04) containing a single U-box and five ARM repeats, lacking a signal peptide and transmembrane domain, and localizing to the nucleus. The BoPUB3L promoter contains multiple cis-acting elements related to light responsiveness, abscisic acid, auxin, and gibberellin responses, as well as meristem-associated expression. BoPUB3L is most highly expressed in the style, followed by sepals, petals, and buds, as confirmed by GUS staining. Transcript abundance increased continuously during the first 30 min after self-pollination and peaked at 15 min, reaching a level 15.65-fold higher than that after cross-pollination. Yeast two-hybrid, pull-down, and BiFC assays further showed that BoPUB3L interacts with the kinase domain of the S-locus receptor kinase (SRK). Together, these results identify BoPUB3L as a previously uncharacterized component of the SI response in B. oleracea and provide new insight into the molecular basis of self-incompatibility in crucifers.

      Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat
      Zhang Xian-Feng, Guo Li-Jian, Li Kang-Chun, Kong Bin-Xue, Liu Yu-Fang, Che Zhuo, Yang De-Long
      Acta Agronomica Sinica. 2026, 52(6):  1711-1727.  doi:10.3724/SP.J.1006.2026.51096
      Abstract ( 305 )   HTML ( 17 )   PDF (8610KB) ( 152 )   Save
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      α/β-Hydrolase domain-containing protein 6 (ABHD6), a key member of the serine hydrolase superfamily, plays important roles in regulating plant growth, development, and responses to environmental stress. However, a systematic genome-wide identification and analysis of the ABHD6 gene family in wheat (Triticum aestivum L.) is still lacking. Here, we integrated phylogenetic analysis, protein sequence characterization, promoter cis-acting element prediction, and haplotype analysis, and identified 18 TaABHD6 family members in the wheat genome. Phylogenetic and structural analyses showed that TaABHD6 proteins are closely related to rice OsABHD6 proteins, and that members within the same clade share highly conserved domains. Promoter analysis revealed that TaABHD6 promoters are enriched in cis-acting elements associated with plant growth and development. By analyzing polymorphisms in TaABHD6 genes and their associations with grain phenotypes, we identified eight genes with distinct haplotypes. Haplotype variation in three genes was significantly associated with grain-related traits; in particular, TaABHD6-5 haplotypes were significantly associated with grain weight, length, width, and thickness. We developed a KASP marker based on an A/T SNP at -1045 bp in the TaABHD6-5 promoter. Validation in 305 wheat accessions showed that this functional marker effectively distinguished two haplotypes: 268 accessions carried TaABHD6-5-HapI, 34 carried TaABHD6-5-HapII, two were heterozygous, and one accession failed genotyping. Association analysis further indicated that TaABHD6-5-HapI is a favorable haplotype for increased grain weight. qRT-PCR showed that TaABHD6-5 was relatively highly expressed in stems and spikes at booting and during early grain development; notably, expression was significantly lower in the favorable haplotype TaABHD6-5-HapI than in TaABHD6-5-HapII, suggesting that TaABHD6-5 may act as a negative regulator of grain weight. Sequence analysis further suggested that TaABHD6-5 is targeted by miR160. Together, these results provide genome-wide evidence for the roles of TaABHD6 genes in wheat grain development and highlight the potential breeding value of superior haplotypes.

      Analysis of transcription factor regulatory networks in two-line male sterile rice seedling roots in response to salt stress
      Zou Yi-Mei, Xu Min, Wang Hai-Yang, Yao Hui, Wang Jia-Feng, Liu Hao, Ren Dai-Sheng
      Acta Agronomica Sinica. 2026, 52(6):  1728-1742.  doi:10.3724/SP.J.1006.2026.52036
      Abstract ( 510 )   HTML ( 11 )   PDF (13483KB) ( 120 )   Save
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      To comprehensively elucidate the transcription factor regulatory network underlying salt-stress responses in rice seedling roots, we used the rice male sterile line Huaxing 166S as material, treated seedling roots with three NaCl concentrations (0, 0.25%, and 0.50%) for one week and recorded phenotypic data. RNA-seq was then used to characterize gene expression patterns, detecting 30,378 genes, of which 26,315 were identified as significantly differentially expressed genes (DEGs). Pathway enrichment analysis showed that, across all three NaCl treatments, DEGs were significantly enriched in secondary metabolism-related pathways. Based on the DEG dataset, we further identified 326 differentially expressed transcription factors and constructed a core interaction network comprising 88 transcription factors. Functional enrichment analysis indicated that this core network was mainly involved in plant hormone signal transduction, the plant MAPK signaling pathway, and the plant circadian rhythm pathway. In addition, quantitative real-time PCR (qPCR) validation showed that, following salt stress, 10 WRKY family genes were specifically highly expressed in rice roots. Together, these transcriptome data across three NaCl concentrations define the gene expression landscape and a core transcription factor interaction network in rice seedling roots, providing a basis for dissecting the molecular regulatory mechanisms of rice root responses to salt stress.

      Genome-wide association analysis and prediction model construction for soybean plant height
      Tang Kuan-Qiang, Li Gong-Yun, Song Mei-Yi, Zhao Xue, Chang Chun-Ling
      Acta Agronomica Sinica. 2026, 52(6):  1743-1756.  doi:10.3724/SP.J.1006.2026.55064
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      Plant height is a key agronomic trait that is closely associated with soybean yield potential and lodging resistance, and it is essential for achieving high and stable yields in soybean production. Here, we conducted a genome-wide association study (GWAS) and genomic selection (GS) analyses of plant height using a diverse panel of 17,148 soybean germplasm accessions with worldwide geographic representation. We identified 139 significant genomic loci associated with plant height, with the candidate gene Dt1 showing the largest genetic effect. In the GS analysis, the BayesA and rrBLUP models outperformed the other models in prediction accuracy. Further comparisons indicated that prediction models based on the top 500-1000 SNPs provided the best balance between predictive performance and cost effectiveness. Collectively, the loci and models identified here support efficient genomic selection for soybean plant height and provide a theoretical basis for molecular design breeding to improve this trait.

      Phenotypic variation analysis of 175 foxtail millet (Setaria italica) germplasm accessions under two environments
      Ma Xiao-Qian, Qin Na, Dai Shu-Tao, Qin Jia-Fan, Li Xiao-Yan, Wang Shu-Ting, Liu Zhong-Ling, Li Jun-Xia
      Acta Agronomica Sinica. 2026, 52(6):  1757-1773.  doi:10.3724/SP.J.1006.2026.54136
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      Breakthroughs in breeding new crop varieties depend strongly on the genetic diversity of germplasm resources. However, research on foxtail millet (Setaria italica) germplasm is often limited by unrepresentative sampling, phenotyping in a single environment, and an overreliance on quantitative indices, all of which constrain the efficient identification of breeding materials. To address these limitations, we evaluated 175 foxtail millet accessions from diverse origins and systematically characterized 21 phenotypic traits at two sites (Luoyang and Zhengzhou, Henan province) in 2023. Comprehensive assessments were performed using correlation analysis, principal component analysis, stepwise regression, and cluster analysis. The aims were to (i) elucidate patterns of phenotypic variation among foxtail millet germplasm, (ii) define core evaluation indicators, and (iii) identify elite accessions adapted to the agroecological conditions of Henan province, thereby providing targeted germplasm resources and a theoretical basis for breeding summer-sown foxtail millet. The results showed substantial diversity among the 175 accessions: the diversity index for nine qualitative traits ranged from 0.086 to 1.218, and that for 12 quantitative traits ranged from 1.173 to 1.399. The coefficients of variation for four quantitative traits differed markedly between sites; plant height showed the smallest variation (followed by grain number per spikelet), whereas 1000-grain weight showed the largest variation (followed by spike diameter). Principal component analysis extracted 14 principal components at a cumulative contribution rate of 90%, capturing most of the phenotypic variation. The mean comprehensive phenotypic score (F value) calculated using the membership function method was 0.538; Yugu 2 had the highest F value (1.078) and the best overall performance, whereas Longgu 25 had the lowest F value (0.079). Stepwise regression produced a model with 11 phenotypic traits (e.g., grain number per spike and spikelet number per spike) as independent variables (R2 = 0.971, P < 0.05), which can serve as a core set of indicators for identifying superior foxtail millet germplasm. Based on F values, cluster analysis grouped the accessions into six clusters; Group II contained two accessions with outstanding agronomic performance and the highest mean F value, indicating their potential as preferred parents for germplasm innovation and hybrid breeding. Overall, the tested foxtail millet germplasm exhibits rich genetic variation and provides diverse material support for the improvement and cultivar development of summer-sown foxtail millet.

      Integrated transcriptomic-metabolomic analysis of wax biosynthesis in castor bean (Ricinus communis L.)
      Wang Ya, Zhao Yi-Ting, Wang Zhou, Yang Jun-Fang, Zhang Hong-Bin, Cao Yue
      Acta Agronomica Sinica. 2026, 52(6):  1774-1787.  doi:10.3724/SP.J.1006.2026.54095
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      This study investigated the molecular regulatory mechanisms and metabolic pathways underlying wax biosynthesis in castor bean (Ricinus communis L.), with a focus on wax-deficient mutants. We performed metabolomic and transcriptomic analyses of the wax-deficient mutant SH8 and its wild type SHA1. Transcriptome profiling identified 610 differentially expressed genes (DEGs) between SH8 and SHA1, including 303 upregulated and 307 downregulated genes. Functional annotation and KEGG enrichment of these DEGs highlighted 12 genes associated with fatty acid synthesis and transport related to wax formation, spanning pathways such as fatty acid degradation, fatty acid elongation, cutin/suberine/wax biosynthesis, and biosynthesis of unsaturated fatty acids. In addition, correlation analysis between DEGs and differential metabolites identified several key genes involved in wax synthesis and metabolism, including CER1, KCS10, LACS1, CYP86A8, and FAR3-like. These results provide a basis for elucidating wax biosynthesis in the castor bean epidermis and support the identification and cloning of key wax-related genes, facilitating the development of castor bean varieties with improved stress tolerance.

      TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY
      Estimation of leaf nitrogen content in dryland forage maize using UAV-based hyperspectral imaging and machine learning
      Liang Jin-Yu, Yin Jia-De, Wang Hong-Li, Zhang Guo-Ping, Hou Hui-Zhi, Dong Bo, Ma Ming-Sheng
      Acta Agronomica Sinica. 2026, 52(6):  1788-1801.  doi:10.3724/SP.J.1006.2026.53085
      Abstract ( 372 )   HTML ( 12 )   PDF (8537KB) ( 161 )   Save
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      This study integrates UAV-based hyperspectral imaging with ensemble learning to identify an optimal spectral estimation model for predicting leaf nitrogen content (LNC) in dryland forage maize, providing a methodological reference for improving production efficiency and quality. The study was conducted on the Loess Plateau in central Gansu province, China, with forage maize as the target crop. Hyperspectral data were acquired using a V185G integrated gimbal hyperspectral imaging system mounted on a UAV. Spectral indices were generated from all possible two-band combinations using original reflectance spectra, first-derivative spectra, and continuum-removed spectra. Six machine-learning algorithms were evaluated, and Voting and Stacking ensemble models were further developed to select the best-performing approach. Transformed spectra substantially strengthened the relationships between spectral indices and LNC compared with the original bands. Among the six individual models, random forest regression (RFR), K-nearest neighbors (KNN), XGBoost, and gradient boosting decision tree (GBDT) achieved relatively high accuracy across maize growth stages, with test-set R2 values of 0.7165-0.7713 and RMSE values of 2.4265-2.8296. These four models were then combined to build the ensemble models, both of which achieved test-set R2 > 0.7459 and RMSE < 2.6358. The Voting ensemble based on first-derivative spectra delivered the best performance (R2 = 0.8152, RMSE = 2.1253), indicating improved predictive accuracy and robustness through model integration. Overall, the Voting-first-derivative spectra (Voting-FDS) model enables rapid estimation of LNC at key growth stages, supporting in-season nutrient management and high-quality production in dryland forage maize.

      Effects of tillage methods and nitrogen application rate on maize yield and soil aggregates in northeastern China under straw returning
      Ma Sheng-Qian, Wang Zhi-Ping, Chen Hao-Tian, Dou Shu-Xian, Zhang Yan, Deng Ai-Xing, Zhang Wei-Jian, Yuan Xiang-Yang, Song Zhen-Wei
      Acta Agronomica Sinica. 2026, 52(6):  1802-1816.  doi:10.3724/SP.J.1006.2026.53066
      Abstract ( 648 )   HTML ( 13 )   PDF (937KB) ( 194 )   Save
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      Northeastern China is a major maize-producing region, but decades of unsustainable tillage practices and excessive nitrogen fertilization have degraded black soils and threatened the sustainable production of maize. Conservation tillage practices based on straw return and reduced or no tillage are key approaches to improving soil quality and maintaining crop productivity, and optimizing nitrogen inputs is essential for enhancing resource-use efficiency. To determine suitable tillage practices and optimal nitrogen rates under straw incorporation, and to promote coordinated improvements in maize yield and soil quality in northeastern China, a fixed-site field experiment was conducted from 2022 to 2024 at Yanjiagang farm, Harbin, Heilongjiang province. The experiment used a split-plot design with tillage as the main-plot factor—conventional tillage (CT), rotary tillage (RT), strip tillage (ST), and no tillage (NT)—and nitrogen rate as the subplot factor: 0 (N0), 135 (N1), 180 (N2), and 225 kg hm-2 (N3) as pure N. Across the three years, mean grain yield ranked ST > CT > RT > NT, although differences among tillage treatments were not significant. Relative to NT, ST increased yield by 14.5% in 2023 and 6.6% in 2024, with no significant differences between ST and CT or RT. Nitrogen rate significantly increased yield (P < 0.001); yield under N2 and N3 were 183.1%-217.2% higher than under N0. No significant tillage×nitrogen interaction was detected for yield. Mean soil bulk density in the 0-20 cm layer followed the order NT > ST > RT > CT, with significant differences among tillage treatments; NT was 20.2%-31.4% higher than CT. Both tillage and nitrogen rate significantly affected aggregate-size distribution, mean weight diameter (MWD), and geometric mean diameter (GMD) (P < 0.05). NT increased the proportion of 0.250-2.000 mm aggregates in the 0-20 cm layer by 23.7%-56.7%, and MWD and GMD ranked NT > RT > ST > CT. The N2 and N3 treatments increased the proportion of 0.250-2.000 mm aggregates by 9.2%-29.7% and decreased the proportion of < 0.053 mm aggregates by 30.3%-51.5%. Correlation analysis showed significant positive associations between tillage practice and nitrogen rate under straw return and maize yield (P < 0.01), and the ST treatment achieved the maximum yield at an estimated N rate of 200.2 kg hm-2. Overall, under straw incorporation, strip tillage combined with 180-225 kg hm-2 N can achieve high maize yield while improving soil aggregation, and is therefore recommended for the cold regions of northeastern China.

      Effects of exogenous hormones on chlorophyll fluorescence parameters and the antioxidant enzyme system in soybean leaves under low-temperature stress
      Jin Yu-He, Wang Xue-Fei, Xu Zhang-Yi-Wa, Miao Yi-Ning, Jiang Yun-Jie, Yi Ying, Miao De-Lin, Zhu Jing-Yi, Zhong Yi-Fan, Chen Ming-Heng, Fang Fang, Liu Peng
      Acta Agronomica Sinica. 2026, 52(6):  1817-1829.  doi:10.3724/SP.J.1006.2026.55068
      Abstract ( 611 )   HTML ( 13 )   PDF (2220KB) ( 117 )   Save
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      Low-temperature stress is a major abiotic constraint on soybean production. To evaluate how exogenous hormones affect chlorophyll fluorescence and the antioxidant defense system in soybean leaves under low-temperature stress, we used two cultivars with contrasting cold tolerance, Mengdou 16 and Heihe 18. Plants were exposed to 4℃ and treated separately with optimal concentrations of abscisic acid (ABA), salicylic acid (SA), melatonin (MT), brassinolide (BR), and jasmonic acid (JA). Their effects on morphology, photosynthetic performance, osmotic adjustment, and antioxidant enzyme activities were assessed after 2, 4, and 6 days. Membership function analysis was used to identify the most effective hormones for enhancing cold tolerance, and response surface methodology was applied to determine an optimal hormone combination. Low-temperature stress caused substantial injury, with Mengdou 16 showing greater cold tolerance and more stable physiological responses than Heihe 18. All hormone treatments alleviated growth inhibition, and the most effective concentrations were 13.93 mg L-1 ABA, 200.62 mg L-1 SA, and 30.30 mg L-1 JA. Hormone application increased plant height, fresh weight, and leaf area, although responses differed between cultivars. Total chlorophyll content increased by up to 97.60% in Mengdou 16 and 108.28% in Heihe 18. In both cultivars, initial fluorescence (F0) and non-photochemical quenching (qN) decreased; photochemical quenching (qP) and electron transport rate (ETR) increased in Mengdou 16, whereas qP in Heihe 18 decreased in some treatments. The maximum reductions in F0 were 34.38% and 24.86% in Mengdou 16 and Heihe 18, respectively, indicating improved photosynthetic energy conversion. Malondialdehyde (MDA) content decreased significantly under all treatments, with maximum reductions of 66.50% (Mengdou 16) and 62.57% (Heihe 18), while proline content increased by up to 24.47% and 19.97%, respectively. Activities of SOD, POD, and CAT were also enhanced, with SOD and CAT peaking at day 4 and POD showing the greatest increase at day 6. Overall, exogenous hormones mitigated low-temperature damage in soybean by improving photochemical performance and strengthening antioxidant and osmotic adjustment systems, providing a theoretical basis and practical guidance for soybean production under low-temperature conditions.

      Effects of tillage methods and nitrogen rates on yield and quality of dryland wheat under one-off irrigation
      Hu Chuan, Zhao Kai-Nan, Huang Xiu-Li, Wu Jin-Zhi, Ren Kai-Ming, Wang He-Zheng, Fu Guo-Zhan, Huang Ming, Li You-Jun
      Acta Agronomica Sinica. 2026, 52(6):  1830-1846.  doi:10.3724/SP.J.1006.2026.51088
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      As China’s High-Standard Farmland Construction Program advances, many dryland wheat fields can now receive a one-off irrigation during the growing season (hereafter, one-off irrigation). To evaluate the effects of tillage practices and nitrogen (N) rates on wheat yield and quality under one-off irrigation, we conducted a two-factor split-plot experiment from 2020 to 2022 at three sites in Luoyang, Henan province (Xiaolangdi town, Mengjin county; Yaling town, Yichuan county; and Xiaojie town, Luoning county). Tillage practice was the main-plot factor—rotary tillage (RT), subsoiling (SS), and plough tillage (PT)—and N rate was the subplot factor at 0 (N0), 120 (N120), 180 (N180), and 240 (N240) kg hm-2. We measured grain yield, protein content and protein fractions, grain Zn content, and key processing-quality traits. Tillage practice and N rate significantly affected wheat yield and quality; their interaction significantly influenced grain yield, protein yield, and the contents of all protein components except albumin. Compared with PT and RT, SS increased grain yield by 6.9% and 12.6%, respectively, and increased protein yield by 7.7% and 14.5%, while generally improving protein content, most protein fractions, processing quality, and grain Zn content (with a few site-year exceptions). Relative to PT, SS increased albumin, globulin, gliadin, and glutenin contents as well as dough development time, stability time, wet gluten content, sedimentation value, extensibility, maximum resistance, and grain Zn content by 21.9%, 19.0%, 12.5%, 8.0%, 18.6%, 28.4%, 8.2%, 26.4%, 10.1%, 14.0%, and 12.6%, respectively; compared with RT, the corresponding increases were 22.0%, 19.6%, 19.7%, 15.0%, 19.5%, 32.8%, 9.1%, 27.8%, 10.3%, 22.6%, and 23.2%, respectively. Across all tillage practices, increasing N rate led to an initial increase followed by a plateau in yield, protein yield, protein fractions, processing-quality traits, and grain Zn content. In most cases, N180 and N240 did not differ significantly, and both outperformed N120. Except for grain yield at the Xiaolangdi site and gliadin content in 2021-2022, SSN180 achieved similar yield, quality, economic returns, and input-output ratio to SSN240, while outperforming the other treatments in most comparisons. Overall, subsoiling combined with 180 kg hm-2 N is recommended for dryland regions where one-off irrigation is available, as it can simultaneously improve wheat yield, quality, and economic benefits.

      Response of wheat tillering and spike formation to nitrogen rate under supplementary irrigation based on soil moisture content
      Gao Pei-Yang, Li Jin-Xuan, Dong Yu-Kui, Shi Yu, Zhang Zhen, Zhang Yong-Li
      Acta Agronomica Sinica. 2026, 52(6):  1847-1858.  doi:10.3724/SP.J.1006.2026.51093
      Abstract ( 288 )   HTML ( 11 )   PDF (1146KB) ( 112 )   Save
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      To elucidate the physiological mechanisms by which nitrogen rate regulates wheat tillering and spike formation under water-saving irrigation with supplemental irrigation guided by soil moisture measurements, we evaluated four nitrogen rates (0, 130, 180, and 230 kg hm-2) in wheat cultivar Jimai 22 from 2020 to 2022. We assessed the cross-sectional area of the tillering node, the photosynthetic capacity of the uppermost fully expanded leaves on the main stem, and 13C-assimilate partitioning among stems and tillers during tillering and spike formation. Compared with 0 and 130 kg hm-2, 180 kg hm-2 significantly increased tillering-node cross-sectional area and trans-zeatin (tZ) content, decreased indole-3-acetic acid (IAA) and abscisic acid (ABA) contents, and enhanced the photosynthetic capacity of the uppermost fully expanded leaves at the overwintering and regreening stages. Total tillers per population at overwintering increased by 35.1% and 14.3% under 180 kg hm-2 relative to 0 and 130 kg hm-2, respectively; at jointing, the corresponding increases were 80.6% and 22%. The allocation of 13Cassimilates to tillers I, II, and III under 180 kg hm-2 was significantly higher than under 0 kg hm-2, and the mean grain yield across the two years was 47.9% and 19.2% higher than that under 0 and 130 kg hm-2, respectively. Increasing the nitrogen rate to 230 kg hm-2 did not further improve these traits compared with 180 kg hm-2, but significantly reduced nitrogen partial factor productivity. Correlation analysis showed that tillering-node cross-sectional area, trans-zeatin content, and net photosynthetic rate of the uppermost fully expanded leaves were significantly and positively correlated with total tillers per population and spike number, and that 13C-assimilate allocation to tillers was significantly and positively correlated with spike number. These results indicate that increasing tillering-node cross-sectional area and trans-zeatin content, enhancing leaf photosynthetic capacity, and promoting assimilate allocation to tillers provide key physiological bases for improving spike number and grain yield. Overall, under the soil-moisture-guided water-saving irrigation regime used here, 180 kg hm-2 was the optimal nitrogen rate for achieving high yield and high nitrogen-use efficiency.

      Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in ‘Jimai 60’ seedlings under combined salinity-alkalinity stress in hydroponics
      Chen Xue-Yan, He Hua-Chuan, Li Zheng-Jia, Dong Xin-Pan, Li Ou-Qi, Liu Xiao-Yun, Li Dan-Ping, Chen Zhi-Wei, Liu Guo-Xia, Lyu Sheng-Yuan, Wu Yin-Ying, Zhao Zhen-Dong, Cao Xin-You, Wan He-Ping
      Acta Agronomica Sinica. 2026, 52(6):  1859-1875.  doi:10.3724/SP.J.1006.2026.51086
      Abstract ( 612 )   HTML ( 9 )   PDF (18156KB) ( 173 )   Save
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      Soil salinization is a major constraint on global agricultural production, substantially reducing wheat yield and threatening food security. Root secretion of organic acids is an important physiological strategy by which plants cope with abiotic stress. However, under combined salt-alkali stress, the temporal dynamics of organic acid secretion in wheat roots and the underlying transcriptional regulatory networks remain poorly understood. Here, the salt-alkali-tolerant wheat cultivar ‘Jimai 60’ was used to systematically characterize time-resolved metabolomic and transcriptomic profiles of root organic acids under combined salt-alkali stress, and key regulatory modules were identified using WGCNA. Combined salt-alkali stress markedly reshaped the composition and abundance of root organic acids. Across four comparison groups, 44 differentially accumulated organic acids were identified, including 12 that were consistently upregulated and 4 that were consistently downregulated. Transcriptome analysis showed that the number of differentially expressed genes increased progressively with longer stress exposure. Genes related to photosynthesis, sexual reproduction, and porphyrin metabolism were persistently activated, whereas cell wall organization and oxidative phosphorylation exhibited stage-specific responses. WGCNA further identified the MEblue, MEturquoise, and MEyellow modules as significantly associated with major organic acid metabolic traits; genes in these modules were mainly enriched in pathways related to energy metabolism, amino acid biosynthesis, and redox homeostasis. Overall, this study delineates the dynamic landscape of root organic acid metabolism and transcriptional regulation in salt-alkali-tolerant wheat (‘Jimai 60’) under combined salt-alkali stress. These findings advance our understanding of the physiological basis of wheat salt-alkali tolerance and provide a foundation, as well as candidate gene resources, for molecular breeding aimed at improving root function and stress resilience.

      Effects of foliar application of exogenous selenium on anthocyanin biosynthesis in tubers of light purple-fleshed potatoes
      Chen Guo-Huan, Zhang Rui, Li Yan-Di, Zhao Jia-Qi, Ren Yong-Tao, Zhang Tian-Ci, Guo Hua-Chun, Li Jun, Yang Fang
      Acta Agronomica Sinica. 2026, 52(6):  1876-1890.  doi:10.3724/SP.J.1006.2026.54127
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      This study investigated how foliar selenium (Se) application affects chlorogenic acid, total phenols, flavonoids, and anthocyanins in light purple-fleshed potatoes, as well as the expression of genes involved in the anthocyanin biosynthesis pathway, with the aim of identifying the optimal external Se concentration and spray frequency to promote anthocyanin accumulation in tubers. The purple-skinned, light purple-fleshed cultivar ‘Diancaishu 104’ was used as the experimental material. Sodium selenite was applied by foliar spraying at three Se concentrations: 0 (distilled water, CK), 5 (Se1), and 10 mg L-1 (Se2), with two application frequencies (2 or 3 sprays). For the two-spray treatment, applications were performed at the tuber formation and tuber bulking stages; for the three-spray treatment, applications were performed at the tuber formation, tuber bulking, and tuber maturation stages, giving a total of five treatments. Chlorogenic acid, total phenols, flavonoids, and anthocyanins were quantified, and the expression of anthocyanin pathway-related genes was analyzed. The results showed that two sprays of Se1 more effectively promoted anthocyanin synthesis in potatoes. Specifically, two foliar sprays of Se1 significantly upregulated StF3'5'H, StCHS, StDFR, StANS, and StMYB113 expression in the skin by 9.11-10.11-fold, and increased StCHS and StDFR expression in the flesh by 2.99-3.72-fold. In contrast, three sprays of Se2 were more effective at increasing chlorogenic acid and total phenols in tubers, raising their contents by 30.12% and 40.80%, respectively, compared with CK. Moreover, Se treatment strengthened the expression correlations among multiple structural genes and transcription factors in the anthocyanin biosynthesis pathway, facilitating the formation of a synergistic regulatory network and thereby promoting anthocyanin accumulation.

      Shading inhibits ear and tassel development of summer maize by altering source-sink balance and regulating carbohydrate metabolism
      Liu En-Bo, Chen Jing, Li Hong-Xing, Yu Ning-Ning, Ren Bai-Zhao, Zhao Bin, Liu Peng, Zhang Ji-Wang
      Acta Agronomica Sinica. 2026, 52(6):  1891-1901.  doi:10.3724/SP.J.1006.2026.53072
      Abstract ( 303 )   HTML ( 16 )   PDF (2133KB) ( 152 )   Save
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      Shading is a major environmental stressor that strongly affects maize growth, development, and yield formation. It is particularly problematic in high-density planting and intercropping systems, where inadequate light interception around the ear can cause substantial yield losses. To examine how shading influences young ear development, field experiments were conducted during the ear development period (jointing stage to tasseling stage) in the summers of 2023 and 2024, with a shading treatment (S) and a natural-light control (CK). Ear morphology, carbohydrate contents in ears, hormone levels, and leaf photosynthetic performance were measured. Shading significantly reduced leaf photosynthetic capacity in summer maize. Relative to CK, leaf sucrose content under shading declined by 65.0%, 24.2%, and 23.0% at V12 (12th leaf stage), V15 (15th leaf stage), and VT (tasseling stage), respectively, accompanied by reductions in plant biomass of 39.5%, 32.3%, and 41.9%. The weakened photosynthetic performance and reduced assimilate production under shading further constrained carbohydrate supply to, and utilization within, developing ears, resulting in significantly lower fructose, glucose, sucrose, and starch contents in both female and male ears. Shading also markedly altered abscisic acid (ABA), auxin (IAA), and cytokinin (CTK) levels in developing ears, leading to delayed ear development, smaller ear length and diameter, and a pronounced reduction in fertile floret number. Overall, shading during the ear stage inhibited normal ear development by limiting assimilate accumulation and disrupting carbohydrate metabolism in young ears, which contributed to a 32.2% reduction in kernels per ear and a 32.8% decrease in yield per plant. These findings clarify the physiological basis of shading-induced yield loss in maize and provide a foundation for breeding shade-tolerant cultivars and optimizing cultivation practices.

      RESEARCH NOTES
      Genome-wide identification and expression analysis of the SPX gene family in rice under phosphorus treatment
      Hu Zhao, Qian Run, Xie Feng-Pu, Ying Su-Ping
      Acta Agronomica Sinica. 2026, 52(6):  1902-1912.  doi:10.3724/SP.J.1006.2026.52044
      Abstract ( 355 )   HTML ( 15 )   PDF (4860KB) ( 166 )   Save
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      This study aimed to systematically identify the rice SPX gene family at the whole-genome level, characterize its evolutionary features and expression patterns, and provide a basis for elucidating SPX functions in phosphorus signal transduction. Bioinformatics approaches were used to identify SPX genes in rice and to analyze their physicochemical properties, phylogenetic relationships, gene structures, conserved motifs, promoter cis-acting elements, and collinearity. Transcriptomic data and quantitative real-time PCR (RT-qPCR) were used to examine tissue-specific expression and responses to phosphorus stress. Six SPX genes were identified and mapped to five chromosomes. Phylogenetic analysis showed that OsSPX1 and OsSPX2 clustered together with 100% bootstrap support, whereas OsSPX3 formed a well-supported subclade with OsSPX5 and OsSPX6. Collinearity analysis identified two segmental duplication pairs (OsSPX1/OsSPX2 and OsSPX5/OsSPX6), and all Ka/Ks values were < 1 (0.19-0.44), indicating strong purifying selection. Gene structure analysis showed that OsSPX members contain 2-3 exons, and conserved motif distributions were highly consistent with the phylogenetic relationships. Promoter analysis identified 135 cis-acting elements, mainly related to hormone, light, and stress responses, with methyl jasmonate- and abscisic acid-responsive elements being the most abundant. RT-qPCR analysis in the ShijinB variety indicated that OsSPX1-OsSPX6 were preferentially expressed in roots, with OsSPX3 also showing relatively high expression in inflorescences and OsSPX1 and OsSPX4 showing relatively high expression in leaves. Under low-phosphorus conditions, OsSPX1, OsSPX2, OsSPX3, OsSPX5, and OsSPX6 were significantly upregulated, with OsSPX3 showing the strongest induction, whereas OsSPX4 was not affected by phosphorus availability. Overall, the rice SPX gene family appears to have expanded via segmental duplication and subsequently evolved under purifying selection; although gene structures and motif compositions are relatively conserved, members show clear tissue specificity and differential responsiveness to phosphorus stress.

Co-sponsored:
the Crop Science Society of China
the Institute of Crop Sciences, CAAS
China Science Publishing & Media Ltd.
Published: Science Press
Editor-in-chief: Wan Jian-min
Associate Editors-in-Chief:
Zhang Xian-long Ding Yan-feng Wang Jian-kang
Xu Ming-liang Liu Lu-xiang Qiu Li-juan
Ni Zhong-fu Zhou Wen-bin Yan Chun-ling
Director of the editorial department:
Yan Chun-ling
CN 11-1809/S
Print ISSN 0496-3490
Online ISSN 2098-0078
Post subscription code: 82-336

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