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作物学报 ›› 2021, Vol. 47 ›› Issue (1): 116-124.doi: 10.3724/SP.J.1006.2021.04087

• 耕作栽培·生理生化 • 上一篇    下一篇

施钙对酸性红壤花生根系内生细菌群落结构的影响

张玮1(), 洪艳云1, 刘登望2, 张博文2, 易图永1,*(), 李林2,*()   

  1. 1湖南农业大学植物保护学院 / 植物病虫害生物学与防控湖南省重点实验室, 湖南长沙 410128
    2湖南农业大学旱地作物研究所, 湖南长沙 410128
  • 收稿日期:2020-04-02 接受日期:2020-09-13 出版日期:2021-01-12 网络出版日期:2020-09-22
  • 通讯作者: 易图永,李林
  • 作者简介:E-mail: 349187910@qq.com
  • 基金资助:
    国家重点研发计划项目(2018YFD1000900)

Effects of calcium application on the structural diversity of endophytic bacterial community in peanut roots under acidic red soil cultivation

ZHANG Wei1(), HONG Yan-Yun1, LIU Deng-Wang2, ZHANG Bo-Wen2, YI Tu-Yong1,*(), LI Lin2,*()   

  1. 1College of Plant Protection, Hunan Agricultural University / Hunan Provincial Key Laboratory for Biology and Control of Plant Pests, Changsha 410128, Hunan, China
    2Institute of Dryland Crops of Hunan Agricultural University, Changsha 410128, Hunan, China
  • Received:2020-04-02 Accepted:2020-09-13 Published:2021-01-12 Published online:2020-09-22
  • Contact: YI Tu-Yong,LI Lin
  • Supported by:
    National Research and Development Program of China(2018YFD1000900)

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摘要：

花生是我国重要的油料作物, 也是嗜钙作物。为了探讨施钙对种植在酸性红壤下的花生根系内生细菌多样性的影响, 本研究对不同处理、不同生育期的花生植株根系内生细菌基因组进行16SrRNA基因V3-V4区深度测序, 分析种植于酸性红壤中施钙处理和对照下花生植株根系微生物群落结构。结果发现, 根系内生菌群落中克雷伯氏菌属(Klebsiella)与肠杆菌属(Enterobacter)在所有样品组中均为优势属且相对丰度较高; 花针期不同处理下假单胞菌属(Pseudomonas)与赖氨酸芽孢杆菌属(Lysinibacillus)在施钙组中相对丰度显著高于对照组, 而劳尔氏属(Ralstonia)的相对丰度在施钙组中显著降低; Network共发生网络分析显示, 施钙组根系内生菌的网络连接相对紧密。综上结果表明, 花生根系内生菌群落组成受施钙处理和植株生长发育共同影响, 施钙能改变根系内生菌群落结构, 提高花生植株应对病原菌侵袭。所得结果为今后通过施钙改良酸性土壤品质进而提高花生植株抗病能力提供了理论参考。

关键词: 花生, 钙, 酸性红壤, 内生细菌, 微生物多样性

Abstract:

Peanut is an important economic crop and calcium-loving crop in China. In order to explore the effect of calcium application on the diversity of endophytic bacteria in peanut roots planted in acid red soil, the genomes of endophytic bacteria in peanut roots of different treatments and different growth stages were deeply sequenced by 16SrRNA gene V3-V4 region, and the root microbial community structure of peanut plants planted in acid red soil and control was analyzed. The results showed that Klebsiella and Enterobacter were the dominant genera with high relative abundance in the endophytic bacterial community from all sample groups. The relative abundance of Pseudomonas and Lysinibacillus were significantly higher than that from the control group, while the relative abundance of Ralstonia decreased significantly in the calcium application groups during the pod-pin stage at P ≤ 0.05. The interaction network analysis showed that the connection of root endophytic bacterial in calcium application group was relatively close. We reasonably inferred that the composition of peanut root endophytic bacterial community was affected by calcium application and plant growing development. Calcium application could change the community structure and improve the ability of peanut to cope with external stress. This study may lay a foundation for improving the quality of acid soil and improving the disease resistance of peanut by applying calcium in the future.

Key words: peanut, calcium, acid red soil, endophytic bacteria, microbial diversity

表1

16S扩增子测序样品分组编号"

生育期
Stage		 不施钙组送样编号
Sample groups without calcium application		 施钙组送样编号
Sample groups with calcium application
苗期 Seeding stage B.R.1, B.R.2, B.R.3 BC.R.1, BC.R.2, BC.R.3
花针期 Acicula forming stage F.R.1, F.R.2, F.R.3 FC.R.1, FC.R.2, FC.R.3
饱果期 Pod maturing stage P.R.1, P.R.2, P.R.3 PC.R.1, PC.R.2, PC.R.3

图1

根系内生菌基因组16S V3-V4的PCR扩增电泳图 样品编号同表1。"

图2

Venn分析与UPGMA聚类 A: 不同样品组根系内生菌差异OTUs韦恩图; B: 不同样品组根系内生菌UPGMA聚类树。样品编号同表1。"

图3

不同样品组花生根系内生菌Top 20物种相对丰度柱形图 样品编号同表1。"

表2

属分类水平下不同生育期不同处理相对丰度百分比Top 10的根系内生菌"

处理
Treatment		 样品编号
Sample group		 优势菌属
Dominant genus		 相对丰度百分比PRA (%) 处理
Treatment		 样品编号
Sample group		 优势菌属
Dominant genus		 相对丰度百分比PRA(%)
苗期不施钙
Seeding stage without calcium		 B.R. Acinetobacter 16.9 苗期施钙 Seeding stage with calcium
 BC.R. Klebsiella 30.1
Klebsiella 16.8 Bacillus 26.3
Enterobacter 7.5 Enterobacter 6.1
Anoxybacillus 5.0 Enhydrobacter 5.1
Herbaspirillum 4.9 Bradyrhizobium 1.8
花针期不施钙
Acicula forming stage without calcium
 F.R. Klebsiella 16.9 花针期施钙
Acicula forming stage with calcium
 FC.R. Klebsiella 37.2
Enterobacter 8.9 Stenotrophomonas 13.1
Acinetobacter 6.7 Pseudomonas 8.7
Pantoea 5.0 Comamonas 8.3
Dyella 3.7 Enterobacter 7.4
饱果期不施钙
Pod maturing stage without calcium
 P.R. Klebsiella 33.4 饱果期施钙
Pod maturing stage with calcium		 PC.R. Klebsiella 30.9
Enterobacter 11.3 Pseudomonas 8.9
Paenibacillus 6.3 Paenibacillus 8.7
Pseudomonas 4.9 Lysinibacillus 8.3
Bacillus 1.7 Bacillus 3.2

图4

花针期相对丰度差异显著微生物属"

图5

不同样品组花生根系内生菌LEfSe分析进化分支图 样品编号同表1。"

图6

不同处理下根系内生菌共发生网络图 A: 施钙样品组根系内生菌关联互作网络图; B: 不施钙样品组根系内生菌关联互作网络图。"

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