欢迎访问作物学报,今天是

作物学报 ›› 2021, Vol. 47 ›› Issue (1): 104-115.doi: 10.3724/SP.J.1006.2021.01031

• 耕作栽培·生理生化 • 上一篇    下一篇

春季低温对小麦花粉育性及粒数形成的影响

高芸1(), 张玉雪1,2, 马泉1, 苏盛楠1,3, 李春燕1,*(), 丁锦峰1, 朱敏1, 朱新开1, 郭文善1   

  1. 1扬州大学江苏省作物遗传生理国家重点实验室培育点 / 粮食作物现代产业技术协同创新中心, 江苏扬州 225009
    2太仓市农业机械技术推广站, 江苏太仓 215499
    3太仓市城厢镇农业技术服务站, 江苏太仓 215499
  • 收稿日期:2020-04-05 接受日期:2020-08-19 出版日期:2021-01-12 网络出版日期:2020-09-10
  • 通讯作者: 李春燕
  • 作者简介:高芸, E-mail: 307057893@qq.com
  • 基金资助:
    国家重点研发计划项目(2018YFD0300802);国家重点研发计划项目(2016YFD0300107);国家自然科学基金项目(31771711);扬州市优秀青年基金项目(YZ2017098)(YZ2017098);江苏省自主创新专项(CX(18)1002);江苏高校优势学科建设工程项目资助

Effects of low temperature in spring on fertility of pollen and formation of grain number in wheat

GAO Yun1(), ZHANG Yu-Xue1,2, MA Quan1, SU Sheng-Nan1,3, LI Chun-Yan1,*(), DING Jin-Feng1, ZHU Min1, ZHU Xin-Kai1, GUO Wen-Shan1   

  1. 1Jiangsu National Key Laboratory Nurturing Center of Crop Genetics and Physiology / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China
    2Taicang Agricultural Machinery Technology Promotion Station, Taicang 215499, Jiangsu, China
    3Taicang Chengxiang Agricultural Technology Service Station, Taicang 215499, Jiangsu, China
  • Received:2020-04-05 Accepted:2020-08-19 Published:2021-01-12 Published online:2020-09-10
  • Contact: LI Chun-Yan
  • Supported by:
    National Key Research and Development Program of China(2018YFD0300802);National Key Research and Development Program of China(2016YFD0300107);National Natural Science Foundation of China(31771711);Yangzhou Science Foundation for Excellent Youths(YZ2017098);Funds for Creative Research of Jiangsu Province(CX(18)1002);Priority Academic Program Development of Jiangsu Higher Education Institutions

摘要:

小麦拔节后遭遇春季低温, 每穗粒数下降, 产量降低。为探明春季低温引起每穗粒数减少的生理原因, 以春性小麦扬麦16和半冬性小麦徐麦30为供试品种, 研究小麦倒二叶出生期(昼夜5℃/-3℃)、孕穗期(昼夜8℃/-1℃)和开花期(昼夜12℃/4℃)分别进行低温处理对小麦花粉育性及每穗粒数形成的影响。结果表明: 倒二叶出生期和孕穗期低温均导致2个小麦品种在二胞花粉期和三胞花粉期花药中的多糖物质淀粉和蛋白质的代谢异常, 绒毡层解体延迟; 孕穗期低温还造成花粉母细胞减数分裂中染色体配对异常、出现微核等情况, 影响正常雄配子体形成; 花粉败育率表现为倒二叶出生期低温处理>孕穗期低温处理>开花期低温处理, 徐麦30败育率高于扬麦16; 3个时期低温均显著降低了2个小麦品种结实小穗数、每小穗结实粒数和每穗粒数, 以倒二叶出生期低温处理穗粒数降低的幅度最大。相关分析表明, 春季低温引起花粉母细胞减数分裂异常、绒毡层延迟解体、花药营养物质供给不足导致的花粉育性下降, 是春季低温导致小麦每穗粒数减少的主要原因。

关键词: 小麦, 低温, 粒数, 花粉育性

Abstract:

Grain yield and the number of grains per ear of wheat decreased under the low temperature after jointing stage. In order to explore the physiological reasons of the decrease of grains number per ear by low temperature in spring, the spring wheat variety Yangmai 16 and the semi-winter wheat variety Xumai 30 were used as tested varieties to analyze the effects of low temperature in spring on the fertility of pollen and the formation of grain numbers at the appearance of the penultimate leaf stage (5℃/-3℃, day/night), booting stage (8℃/-1℃, day/night), and anthesis stage (12℃/4℃, day/night). The results showed that low temperature at the appearance of the penultimate leaf stage and booting stage resulted in abnormal metabolism of starch and protein in the anther both at the binuclear and tri-nuclear pollen stages and delayed degradation of the tapetum. The low temperature at the booting stage caused abnormal meiosis of pollen mother cells, abnormalities in chromosome pairing, which affected the formation of male gametophyte. The abortion rate of pollen was more significantly increased under low temperature at the appearance of the penultimate leaf stage than that at the booting stage, and it was the minimum at anthesis stage. The abortion rate of pollen in Xumai 30 was higher than that in Yangmai 16. The low temperature at three stages had significant effects on the number of spikelets, the number of fertile spikelets and the number of grains per spikelet. The effect of low temperature at the appearance of the penultimate leaf stage on grains number per spikelet was the most significant. There was a significant positive correlation between the number of grains per spike and pollen fertility, which was affected by abnormal meiosis, delayed degradation of the tapetum, and undersupply of nutriment for anther development. Therefore, the decline of pollen fertility caused by low temperature in spring is the main reason for the decrease of grains number per spike.

Key words: wheat, cold stress, grain number, pollen fertility

图1

孕穗期低温处理48 h对小麦花粉母细胞减数分裂的影响(100×) 1: 扬麦16对照后期I; 2: 扬麦16对照末期I; 3: 扬麦16对照末期II; 4: 扬麦16后期I (箭头示落后染色体); 5: 扬麦16末期I (箭头示微核); 6: 扬麦16末期II (箭头示微核); 7: 徐麦30后期I (箭头示染色体桥); 8: 徐麦30后期I (箭头示落后染色体); 9: 徐麦30末期II (箭头示子细胞大小不一致)。自然生长条件下, 两小麦品种花粉母细胞减数分裂过程基本一致, 本文用扬麦16自然生长的减数分裂图片为对照。"

图2

不同时期低温处理的小麦花药细胞化学观察(二胞花粉期淀粉染色) A: 扬麦16自然生长对照; B: 扬麦16倒二叶出生期低温处理48 h; C: 扬麦16孕穗期低温处理48 h; D: 徐麦30自然生长对照; E: 徐麦30倒二叶出生期低温处理48 h; F: 徐麦30孕穗期低温处理48 h; Ep: 表皮; En: 药室内壁; ML: 中层; M: 小孢子母细胞; P: 花粉; T: 绒毡层。"

图3

不同时期低温处理的小麦花药细胞化学观察(三胞花粉期淀粉染色) A: 扬麦16自然生长对照; B: 扬麦16倒二叶出生期低温处理48 h; C: 扬麦16孕穗期低温处理48 h; D: 徐麦30自然生长对照; E: 徐麦30倒二叶出生期低温处理48 h; F: 徐麦30孕穗期低温处理48 h; Ep: 表皮; En: 药室内壁; ML: 中层; M: 小孢子母细胞; P: 花粉; T: 绒毡层。"

图4

不同时期低温处理的小麦花药细胞化学观察(二胞花粉期蛋白染色) A: 扬麦16自然生长对照; B: 扬麦16倒二叶出生期低温处理48 h; C: 扬麦16孕穗期低温处理48 h; D: 徐麦30自然生长对照; E: 徐麦30倒二叶出生期低温处理48 h; F: 徐麦30孕穗期低温处理48 h; Ep: 表皮; En: 药室内壁; ML: 中层; M: 小孢子母细胞; P: 花粉; T: 绒毡层。"

图5

不同时期低温处理的小麦花药细胞化学观察(三胞花粉期蛋白染色) A: 扬麦16自然生长对照; B: 扬麦16倒二叶出生期低温处理48 h; C: 扬麦16孕穗期低温处理48 h; D: 徐麦30自然生长对照; E: 徐麦30倒二叶出生期低温处理48 h; F: 徐麦30孕穗期低温处理48 h; Ep: 表皮; En: 药室内壁; ML: 中层; M: 小孢子母细胞; P: 花粉; T: 绒毡层。"

表1

春季不同时期低温对小麦花粉粒活性的影响"

年度
Year
低温处理
Low-temperature treatment
处理时长
Time (h)
扬麦16 Yangmai 16 徐麦30 Xumai 30
败育率
Abortion rate (%)
升幅
Increase (%)
败育率
Abortion rate (%)
升幅
Increase (%)
2015-2016 倒二叶出生期 24 25.5 b 18.6 26.2 b 18.8
The appearance of the penultimate leaf stage (5℃/-3℃) 48 28.4 a 21.5 29.6 a 22.2
孕穗期 24 15.3 d 8.5 17.5 d 10.1
Booting stage (8℃/-1℃) 48 19.2 c 12.3 19.8 c 12.4
开花期 24 8.0 e 1.2 8.7 e 1.3
Anthesis stage (12℃/4℃) 48 13.7 d 6.9 16.6 d 9.2
自然生长对照 Control 6.8 e 7.4 e
FF-value 121.24** 154.35**
2016-2017 倒二叶出生期 24 23.6 b 18.2 24.2 b 18.3
The appearance of the penultimate leaf stage (5℃/-3℃) 48 25.2 a 19.8 26.6 a 20.6
孕穗期 24 13.6 d 8.2 14.6 d 8.7
Booting stage (8℃/-1℃) 48 15.4 c 10.0 16.8 c 10.9
开花期 24 7.0 f 1.6 8.0 f 2.0
Anthesis stage (12℃/4℃) 48 9.8 e 4.4 10.2 e 4.3
自然生长对照 Control 5.4 g 6.0 g
FF-value 245.51** 215.02**

表2

春季不同时期低温对小穗退化的影响(2015--2017)"

图6

小麦花粉败育率与退化小穗数的关系"

[1] Augspurger C K. Reconstructing patterns of temperature, phenology, and frost damage over 124 years: spring damage risk is increasing. Ecology, 2013,94:41-50.
doi: 10.1890/12-0200.1 pmid: 23600239
[2] Zheng B Y, Chapman S C, Christopher J T, Frederiks T M, Chenu K. Frost trends and their estimated impact on yield in the Australian wheat belt. J Exp Bot, 2015,66:3611-3623.
doi: 10.1093/jxb/erv163 pmid: 25922479
[3] Barlow K M, Christy B P, O’Leary G J, Riffkin P A, Nuttall J G. Simulating the impact of extreme heat and frost events on wheat crop production: a review. Field Crops Res, 2015,171:109-119.
[4] 罗新兰, 张彦, 孙忠富, 杜克明, 宋广树. 黄淮平原小麦霜冻害时空分布特点的研究. 中国农学通报, 2011,27(18):45-50.
Luo X L, Zhang Y, Sun Z F, Du K M, Song G S. Spatial and temperature distribution of winter wheat frost injury in Huanghuai plain. Chin Agric Sci Bull, 2011,27(18):45-50 (in Chinese with English abstract).
[5] 张永阁. 柘城县小麦冻害发生原因及防治措施. 河南农业, 2016, (1):45.
Zhang Y G. Reasons and protective measures of wheat frost damage in Zhecheng. Henan Agron, 2016, (1):45 (in Chinese).
[6] 赵刚, 申双和, 褚荣浩. 江苏省倒春寒发生程度评估. 江苏农业科学, 2018,46(6):243-247.
Zhao G, Shen S H, Chu R H. Assessment of the incidence of cold spring in Jiangsu. Jiangsu Agric Sci, 2018,46(6):243-247 (in Chinese).
[7] 王葳葳, 刘君圣, 徐迎菊. 2013年春季霜冻对兴化市冬小麦生长的影响及应对补救措施. 现代农业科技, 2014, (16):225-226.
Wang W W, Liu J S, Xu Y J. Effect of spring frost on the growth of winter wheat and remedial techniques in Xinghua in 2013. Morden Agric Sci Tech, 2014, (16):225-226 (in Chinese).
[8] 蔡剑, 姜东. 气候变化对中国冬小麦生产的影响. 农业环境科学学报, 2011,30:1726-1733.
Cai J, Jiang D. The effect of climate change on winter wheat production in China. J Agro-Environ Sci, 2011,30:1726-1733 (in Chinese with English abstract).
[9] 冯伟森, 张学品, 吴少辉, 高海涛, 张灿军. 不同播期对洛旱7号小麦幼穗分化及产量的影响. 河南农业科学, 2011,40(10):32-34.
Feng W S, Zhang X P, Wu S H, Gao H T, Zhang C J. Effects of different sowing dates on spike characteristics and yield of Luohan 7. J Henan Agric Sci, 2011,40(10):32-34 (in Chinese with English abstract).
[10] 李春燕, 徐雯, 刘立伟, 雷晓伟, 杨景, 周冬冬, 朱新开, 郭文善. 药隔至开花期低温对小麦产量和生理特性的影响. 麦类作物学报, 2016,36(1):77-85.
Li C Y, Xu W, Liu L W, Lei X W, Yang J, Zhou D D, Zhu X K, Guo W S. Effect of short-time low temperature from anther connective stage to anthesis on wheat yield and physiological characteristics. J Triticeae Crops, 2016,36(1):77-85 (in Chinese with English abstract).
[11] 张自阳, 王智煜, 王斌, 王志伟, 朱启迪, 霍云风, 茹振钢, 刘明久. 春季穗分化阶段低温处理对不同小麦品种幼穗结实性及生理特性的影响. 华北农学报, 2019,34(4):130-139.
Zhang Z Y, Wang Z Y, Wang B, Wang Z W, Zhu Q D, Huo Y F, Ru Z G, Liu M J. Effects of low temperature treatment at spring spike differentiation stage on young ear fruiting and physiological characteristics of different wheat varieties. Acta Agric Boreali Sin, 2019,34(4):130-139 (in Chinese with English abstract).
[12] Thakur P, Kumar S, Malik J A, Berger J D, Nayyar H. Cold stress effects on reproductive development in grain crops: an overview. Environ Exp Bot, 2010, 67:429-443.
[13] 王亚梁, 张玉屏, 朱德峰, 向镜, 武辉, 陈惠哲, 张义凯. 水稻穗分化期高温胁迫对颖花退化及籽粒充实的影响. 作物学报, 2016,42:1402-1410.
Wang Y L, Zhang Y P, Zhu D F, Xiang J, Wu H, Chen H Z, Zhang Y K. Effect of heat stress on spikelet degeneration and grain filling at panicle initiation period of rice. Acta Agron Sin, 2016,42:1402-1410 (in Chinese with English abstract).
[14] 王俊生, 张改生, 原蕾, 张明珠, 牛娜, 马守才, 叶景秀. 化学杂交剂诱导的小麦生理型雄性不育花药的活性氧代谢. 西北植物学报, 2009,29:1351-1357.
Wang J S, Zhang G S, Yuan L, Zhang M Z, Niu N, Ma S C, Ye J X. Metabolism of reactive oxygen species of physiological male-sterile anther induced by chemical hybrid agent in wheat. Acta Bot Boreali-Occident Sin, 2009,29:1351-1357 (in Chinese with English abstract).
[15] 蒙立颖, 石晓艺, 胡甘, 齐智, 宋喜悦. K型温敏雄性不育小麦KTM3315A的鉴定及花粉败育特点的初步分析. 中国农业大学学报, 2015,20(5):1-8.
Meng L Y, Shi X Y, Hu G, Qi Z, Song X Y. Identification and characteristics of KTM3315A, a thermos-sensitive male sterile line with Aegilops kotschyi cytoplasm. J China Agric Univ, 2015,20(5):1-8 (in Chinese with English abstract).
[16] 马翎健, 司美茹, 宋喜悦, 胡银刚, 奚亚军, 何蓓如, 刘曙东. 牡山羊草细胞质小麦核代换系酶活性及其与花粉败育的关系. 麦类作物学报, 2001,21(3):35-37.
Ma L J, Si M R, Song X Y, Hu Y G, Xi Y J, He P R, Liu S D. Relationship between microspore abortion and activities of superoxide dismutase (SOD) and catalase (CAT) of wheat nuclear sustitutional lines in Ae. juvenalis cytoplasm. J Triticeae Crops, 2001,21(3):35-37 (in Chinese with English abstract).
[17] 巨岚, 朱启迪, 张改生, 张姣, 于永昂, 刘红占, 牛娜, 王军卫. 小麦生理型雄性不育系中天冬氨酸蛋白酶与绒毡层代谢的相关性分析. 核农学报, 2018,32:430-437.
Ju L, Zhu Q D, Zhang G S, Zhang J, Yu Y A, Liu H Z, Niu N, Wang J W. The research on the correlation between aspartic proteinase and anther tapetum in physiological male sterility wheat. J Nucl Agric Sci, 2018,32:430-437 (in Chinese with English abstract).
[18] 张艳, 何勇, 李建雄, 田志宏. 绒毡层发育和激素对拟南芥育性的影响. 安徽农学通报, 2017,23(6):34-38.
Zhang Y, He Y, Li J X, Tian Z H. Effects of tapetal development and hormones on fertility of arabidopsis thaliana. Anhui Agric Sci Bull, 2017, 23(6):34-38 (in Chinese with English abstract).
[19] 宋国琦, 胡银岗, 林凡云, 董普辉, 马翎健, 宋喜悦, 何蓓如. YS型小麦温敏雄性不育系A3017控温条件下的花粉育性比较. 麦类作物学报, 2006,26(1):17-20.
Song G Q, Hu Y G, Lin F Y, Dong P H, Ma L J, Song X Y, He B R. Comparison on the pollen fertility of YS type thermo-sensitive male-sterile wheat line A3017 under artificial temperature conditions. J Triticeae Crops, 2006,26(1):17-20 (in Chinese with English abstract).
[20] 李东霄, 李淦, 冯素伟, 茹振钢. 温敏核不育小麦可育和不育花药的细胞化学观察. 作物学报, 2013,39:878-884.
Li D X, Li G, Feng S W, Ru Z G. Cytochemical observation of fertile and sterile anthers of thermo-sensitive genic male-sterile wheat. Acta Agron Sin, 2013,39:878-884 (in Chinese with English abstract).
[21] 李东霄, 邓小莉, 冯素伟, 徐龙龙, 茹振钢. 温敏核不育小麦可育和败育花粉的超微结构观察. 中国细胞生物学学报, 2013,35:1119-1125.
Li D X, Deng X L, Feng S W, Xu L L, Ru Z G. Ultrastructural observation of fertile and sterile pollen grains of a thermo-sensitive genic male-sterile wheat. Chin J Cell Biol, 2013,35:1119-1125 (in Chinese with English abstract).
[22] 刘海英, 甄俊琦, 胡铁柱, 茹振钢, 李珍, 胡雪寒, 邢晨涛, 高远. 小麦温敏雄性不育系BNS366小孢子发育和花粉育性检测方法研究. 麦类作物学报, 2018,38:379-385.
Liu H Y, Zhen J Q, Hu T Z, Ru Z G, Li Z, Hu X H, Xing C T, Gao Y. Study on microspore development and pollen fertility detection of thermos-sensitive male-sterile wheat line BNS366. J Triticeae Crops, 2018,38:379-385 (in Chinese with English abstract).
[23] 韩笑冰, 利容千, 徐乃瑜, 王建波, 徐祖元. 小麦不同胞质不育系花粉败育的细胞学比较研究. 作物学报, 1996,22:646-651.
Han X B, Li R Q, Xu N Y, Wang J B, Xu Z Y. A comparative cytological study on the pollen abortion of different cytoplasmic male sterile lines of wheat. Acta Agron Sin, 1996,22:646-651 (in Chinese with English abstract).
[24] Barton D A, Cantrill L C, Law A M K, Phillips C G, Sutton B G, Overall R L. Chilling to zero degrees disrupts pollen formation but not meiotic microtubule arrays in Triticum aestivum L. Plant Cell Environ, 2014,37:2781-2794.
pmid: 24762030
[25] 赵莎, 姚家玲. 光敏核不育水稻花粉发育的细胞学研究与PCD检测. 华中农业大学学报, 2007,26:283-288.
Zhao S, Yao J L. Cytological study and PCD assay on pollen development of photoperiod sensitive genic male sterile rice. J Huazhong Agric Univ, 2007,26:283-288 (in Chinese with English abstract).
[26] 王瑞霞, 闫长生, 张秀英, 孙果忠, 钱兆国, 亓晓蕾, 牟秋焕, 肖世和. 春季低温对小麦产量和光合特性的影响. 作物学报, 2018,44:288-296.
Wang R X, Yan C S, Zhang X Y, Sun G Z, Qian Z G, Qi X L, Mou Q H, Xiao S H. Effect of low temperature in spring on yield and photosynthetic characteristics of wheat. Acta Agron Sin, 2018,44:288-296 (in Chinese with English abstract).
[27] Li X N, Pu H C, Liu F L, Zhou Q, Cai J, Dai T B, Cao W X, Jiang D. Winter wheat photosynthesis and grain yield responses to spring freeze. Agron J, 2015,107:1002-1010.
[28] Liu L L, Ji H T, An J P, Shi K J, Ma J F, Liu B, Tang L, Cao W X, Zhu Y. Response of biomass accumulation in wheat to low-temperature stress at jointing and booting stages. Environ Exp Bot, 2019,157:46-57.
doi: 10.1016/j.envexpbot.2018.09.026
[29] Prášil I T, Prášilová P, Pánková K. The relationship between vernalization requirement and frost tolerance in substitution lines of wheat. Biol Plant, 2005,49:195-200.
[30] 刘伟华, 邱博, 罗红兵. 花药绒毡层发育和花粉母细胞减数分裂相关基因研究进展. 作物研究, 2015,29:311-316.
Liu W H, Qiu B, Luo H B. Advances in genes related to tapetum development and microsporocyte meiosis in anther. Crop Res, 2015,29:311-316 (in Chinese with English abstract).
[31] Chen C B, Zhang W, Timofejeva L, Gerardin Y, Ma H. The arabidopsis ROCK-N-ROLLERS gene encodes a homolog of the yeast ATP-dependent DNA helicase MER3 and is required for normal meiotic crossover formation. Plant J, 2005,43:321-334.
doi: 10.1111/j.1365-313X.2005.02461.x pmid: 16045469
[32] Johnson K L, Ingram G C. Sending the right signals: regulating receptor kinase activity. Curr Opin Plant Biol, 2005,8:648-656.
pmid: 16183327
[33] Stracke R, Werber M, Weisshaar B. The R2R3-MγB gene family in Arabidopsis thaliana. Curr Opin Plant Biol, 2001,4:447-456.
[34] Ito T, Shinozaki K. The MALE STERILITY1 gene of Arabidopsis, encoding a nuclear protein with a PHD-finger motif, is expressed in tapetal cells and is required for pollen maturation. Plant Cell Physiol, 2002,43:1285-1292.
doi: 10.1093/pcp/pcf154 pmid: 12461128
[35] 刘菲, 马晓飞, 李艳飞, 王爱芳, 靳凤, 马翎健. 两个育性相关基因在几类小麦雄性不育材料中的表达研究. 西北植物学报, 2012,32:1731-1735.
Liu F, Ma X F, Li Y F, Wang A F, Jin F, Ma L J. Expression analysis of two genes related to male fertility in a few types of male sterile wheat. Acta Bot Boreali-Occident Sin, 2012,32:1731-1735 (in Chinese with English abstract).
[36] Zhang W J, Wang J Q, Huang Z L, Mi L, Xu K F, Wu J J, Fan Y H, Ma S Y, Jiang D G. Effects of low temperature at booting stage on sucrose metabolism and endogenous hormone contents in winter wheat spikelet. Front Plant Sci, 2019,10:498.
doi: 10.3389/fpls.2019.00498 pmid: 31057594
[37] Ji H T, Xiao L J, Xia Y M, Song H, Liu B, Tang L, Cao W X, Zhu Y, Liu L L. Effects of jointing and booting low temperature stresses on grain yield and yield components in wheat. Agric ForMeteorol, 2017,243:33-42.
[38] Subedi K D, Gregory P J, Summerfield R J, Gooding M J. Cold temperatures and boron deficiency caused grain set failure in spring wheat (Triticum aestivum L.). Field Crops Res, 1998,57:277-288.
[39] Subedi K D, Gooding M J, Gregory P J. Cultivar variation in boron accumulation and grain set in wheat under the influence of cold temperature. Ann Appl Biol, 2001,138:97-101.
[1] 胡文静, 李东升, 裔新, 张春梅, 张勇. 小麦穗部性状和株高的QTL定位及育种标记开发和验证[J]. 作物学报, 2022, 48(6): 1346-1356.
[2] 郭星宇, 刘朋召, 王瑞, 王小利, 李军. 旱地冬小麦产量、氮肥利用率及土壤氮素平衡对降水年型与施氮量的响应[J]. 作物学报, 2022, 48(5): 1262-1272.
[3] 冯亚, 朱熙, 罗红玉, 李世贵, 张宁, 司怀军. 马铃薯StMAPK4响应低温胁迫的功能解析[J]. 作物学报, 2022, 48(4): 896-907.
[4] 杜浩, 程玉汉, 李泰, 侯智红, 黎永力, 南海洋, 董利东, 刘宝辉, 程群. 利用Ln位点进行分子设计提高大豆单荚粒数[J]. 作物学报, 2022, 48(3): 565-571.
[5] 付美玉, 熊宏春, 周春云, 郭会君, 谢永盾, 赵林姝, 古佳玉, 赵世荣, 丁玉萍, 徐延浩, 刘录祥. 小麦矮秆突变体je0098的遗传分析与其矮秆基因定位[J]. 作物学报, 2022, 48(3): 580-589.
[6] 冯健超, 许倍铭, 江薛丽, 胡海洲, 马英, 王晨阳, 王永华, 马冬云. 小麦籽粒不同层次酚类物质与抗氧化活性差异及氮肥调控效应[J]. 作物学报, 2022, 48(3): 704-715.
[7] 刘运景, 郑飞娜, 张秀, 初金鹏, 于海涛, 代兴龙, 贺明荣. 宽幅播种对强筋小麦籽粒产量、品质和氮素吸收利用的影响[J]. 作物学报, 2022, 48(3): 716-725.
[8] 宋仕勤, 杨清龙, 王丹, 吕艳杰, 徐文华, 魏雯雯, 刘小丹, 姚凡云, 曹玉军, 王永军, 王立春. 东北主推玉米品种种子形态及贮藏物质与萌发期耐冷性的关系[J]. 作物学报, 2022, 48(3): 726-738.
[9] 马红勃, 刘东涛, 冯国华, 王静, 朱雪成, 张会云, 刘静, 刘立伟, 易媛. 黄淮麦区Fhb1基因的育种应用[J]. 作物学报, 2022, 48(3): 747-758.
[10] 王洋洋, 贺利, 任德超, 段剑钊, 胡新, 刘万代, 郭天财, 王永华, 冯伟. 基于主成分-聚类分析的不同水分冬小麦晚霜冻害评价[J]. 作物学报, 2022, 48(2): 448-462.
[11] 陈新宜, 宋宇航, 张孟寒, 李小艳, 李华, 汪月霞, 齐学礼. 干旱对不同品种小麦幼苗的生理生化胁迫以及外源5-氨基乙酰丙酸的缓解作用[J]. 作物学报, 2022, 48(2): 478-487.
[12] 徐龙龙, 殷文, 胡发龙, 范虹, 樊志龙, 赵财, 于爱忠, 柴强. 水氮减量对地膜玉米免耕轮作小麦主要光合生理参数的影响[J]. 作物学报, 2022, 48(2): 437-447.
[13] 马博闻, 李庆, 蔡剑, 周琴, 黄梅, 戴廷波, 王笑, 姜东. 花前渍水锻炼调控花后小麦耐渍性的生理机制研究[J]. 作物学报, 2022, 48(1): 151-164.
[14] 孟颖, 邢蕾蕾, 曹晓红, 郭光艳, 柴建芳, 秘彩莉. 小麦Ta4CL1基因的克隆及其在促进转基因拟南芥生长和木质素沉积中的功能[J]. 作物学报, 2022, 48(1): 63-75.
[15] 韦一昊, 于美琴, 张晓娇, 王露露, 张志勇, 马新明, 李会强, 王小纯. 小麦谷氨酰胺合成酶基因可变剪接分析[J]. 作物学报, 2022, 48(1): 40-47.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!