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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (6): 1038-1043.doi: 10.3724/SP.J.1006.2009.01038


Detection of Quantitative Trait Loci for Plant Height Using an Immortalized F2 Population in Wheat

WANG Yan,LI Zhuo-Kun,TIAN Ji-Chun*   

  1. Group of Quality Wheat Breeding of Key Laboratory of Crop Biology/Shandong Agricultural University Tai'an 271018,China
  • Received:2008-11-27 Revised:2009-02-17 Online:2009-06-12 Published:2009-04-16
  • Contact: TIAN Ji-Chun,E-mail:jctian@sdau.edu.cn;Tel:0538-8242040


To study the genetic mechanism of wheat plant height, a set of doubled haploid (DH) lines were used to construct an immortalized F2 (IF2) population comprising 168 different crosses. The IF2 population was evaluated for plant height in 2007 cropping seasons in Tai’an and Liaocheng, Shandong province. Linkage map was constructed with 324 SSR markers covering the whole wheat genome, including 284 SSR, 37 ESTs loci, 1 ISSR loci and 2 HMW-GS loci, was constructed. This linkage map covered a total length of 2 485.7 cM with an average distance of 7.67 cM between adjacent markers. QTL analyses were performed using the software QTLNetwork version 2.0 based on the mixed linear model at P < 0.05. Four additive QTLs, 1 dominance QTL and pair of epistatic QTLs were detected, the total QTL effects detected for the plant height explained 20% of the phenotypic variation. One QTL qPh4D for plant height was identified on chromosome 4D, was identified on chromosome 2D, explaining 7.5% of the phenotypic variances. Dominance effect loci qPh2D was identified on chromosome 2D, explaining 1.6% of the phenotypic variances;Epistatic effects of loci was identified on chromosome 5B–6D, explaining 1.7% of the phenotypic variances . The results indicate additive effects, dominance effects and epistatic effects are important in genetics of wheat for plant height, which are also subjected to environmental modifications. These results further demonstrate that the use of IF2 groups QTL positioning research methods contribute to the molecular marker-assisted breeding.

Key words: Common wheat, IF2, Plant height, QTL

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