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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (05): 718-729.doi: 10.3724/SP.J.1006.2017.00718

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity Analysis of Potato varieties

DUAN Shao-Guang,JIN Li-Ping*,LI Guang-Cun,BIAN Chun-Song,XU Jian-Fei,HU Jun,QU Dong-Yu   

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture, Beijing 100081, China
  • Received:2016-10-18 Revised:2017-01-21 Online:2017-05-12 Published:2017-02-20
  • Contact: Jin Liping, E-mail: jinliping@caas.cn E-mail:duanshaoguang@caas.cn
  • Supported by:

    The work was supported by the National Key Technology Support Program of China during the Twelfth Five-year Plan Period (2012BAD02B05) and the China Agriculture Research System (CARS10).

Abstract:

Based on the 16 phenotype traits, we performed UPGMA cluster analysis for 454 potato accessions. All the accessions were grouped into two clusters A1 and A at the Euclidean distance of 14.66. The cluster A1 was further grouped into two subclusters A11 and A12 at the Euclidean distance of 12.74. All the accessions were grouped into nine clusters (A, B, C, H, D, E, F, G, and I) at the Euclidean distance of 11.73, and the cluster I was the biggest group, accounting for 57.5% of all the accessions. The cluster analysis more accurately revealed the morphological difference among potato materials, and distinguished the parental materials with different ecological types and genetic differences. Meanwhile, the genetic diversity of 559 potato accessions from worldwide was analyzed with 36 pairs of SSR primers. A total of 134 polymorphic alleles were amplified. Polymorphic alleles were amplified by each pair of primer ranging from one to seven, with a mean of 3.72. The fragment size amplified varied from 106 to 308 bp. The polymorphic information content values (PIC) were from 0.1545 to 0.7743 with a mean of 0.5783, which indicated SSR markers can reflect more abundant genetic diversity information in potato varieties. The further phylogenetic tree analysis showed that all 559 accessions were clustered into three groups. Group I was a mixed one, containing 133 (23.8%) materials from almost all regions. Group II was one mostly gathered by accessions from Europe, North America, Northeast and Northwest regions in China. There were 187 materials in this group, accounting for 33.5% of all. Group III consisted of 239 accessions (42.8%), mainly distributing in North America, South America, Northeast and Southwest regions in China. The clustering results based on phenotypic traits were similar to those based on SSR markers, both highly relating to geographic location, indicating that both method should be combined to use in potato genetic polymorphism evaluation.

Key words: Potato, Germplasm, Agronomic trait, SSR maker, Genetic diversity

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