以SSR标记对普通菜豆抗炭疽病基因定位

doi:10.3724/SP.J.1006.2009.00432

摘要/Abstract

摘要：

由菜豆炭疽菌引起的菜豆炭疽病是危害我国菜豆生产的主要病害之一, 鉴定和发掘新的抗病基因对于菜豆抗病育种具有十分重要的意义。以来自安第斯基因库的我国菜豆抗炭疽病地方品种红花芸豆与感病地方品种京豆杂交的F₂群体为试验材料, 通过人工接种菜豆炭疽菌81号小种进行抗病性鉴定, 发现该分离群体中抗病植株数与感病植株数符合3∶1的分离比例, 确定红花芸豆对菜豆炭疽菌81号小种的抗性由显性单基因控制, 将此基因命名为Co-F2533。用分离群体分组分析法(BSA)和微卫星多态性分析(SSR)技术对红花芸豆中的抗炭疽病基因进行分子标记鉴定, 用Mapmaker3.0计算标记与目的基因间的遗传距离, 发现B6连锁群上的4个SSR标记BM170、Clon1429、BMD37、Clon410与抗炭疽病基因Co-F2533连锁, 遗传距离分别为6.6、18.4、20.9和30.9 cM, 这些SSR标记与Co-F2533基因在B6连锁群上的排列顺序为Clon1429-Co-F2533- BM170-BMD37-Clon410。根据基因所在连锁群的位置、抗病基因的基因库来源可知Co-F2533是一个新的来源于安第斯基因库的抗炭疽病基因。

关键词: 普通菜豆, 菜豆炭疽病, 抗病基因, SSR标记

Abstract:

Anthracnose (Colletotrichum lindemuthianum) is one of the most important diseases of common bean throughout the world. Resistant cultivars utilization is the most economic, effective and environment harmless method to control this disease. However, disease resistance in the host is unsustainable due to the high variability of anthracnose. So it is necessary to explore the new resistance genes persistently. Among the 11 reported resistant loci, 10 of them originated from Mesoamerican gene pool and only one locus named Co-1 from Andean gene pool. The objective of this study was to identify the new Andean gene resistant to anthracnose in the Chinese common bean landraces. 108 F₂ plants derived from a cross between common bean Red flower (R) and common bean Jing (S) were inoculated with anthracnose race 81. The resistant individuals grew well without obvious disease symptoms but the susceptible plants have many disease spots on the leaves and stems, and then died gradually. The segregation ratio of resistant to susceptible plants in F₂ population was 3:1 based on chi-square test, indicating that the resistance of Red flowercommon bean to anthracnose race 81 was controlled by a single dominant gene, named as Co-F2533 temporarily. Four SSR markers were detected on B6 linkage group of common bean that linked to the resistance gene Co-F2533 with the distance of 6.6, 18.4, 20.9, and 30.9 cM respectively. The position of the resistance gene and the markers on the chromosome were as follows: Clon1429, Co-F2533, BM170, BMD37, and Clon410. A linkage map was constructedwith Mapdraw V2.1. Since the absent of anthracnose resistance genes on B6 linkage group, it is proposed that Co-F2533 is a novel anthracnose resistance gene in Chinese resistant landrace Red flower from Andean gene pool, which will be useful in the anthracnose resistance breeding for common bean.

Key words: Common bean, Anthracnose, Resistant gene, SSR marker

王坤;王晓鸣;朱振东;赵晓彦;张晓艳;王述民. 以SSR标记对普通菜豆抗炭疽病基因定位[J]. 作物学报, 2009, 35(3): 432-437.

WANG Kun;WANG Xiao-Ming;ZHU Zhen-Dong;ZHAO Xiao-Yan;WANG Shu-Min. Mapping of a Novel Anthracnose Resistance Gene Using SSR Markers in Common Bean(Phaseolus vulgaris L.)[J]. Acta Agron Sin, 2009, 35(3): 432-437.

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