欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2010, Vol. 36 ›› Issue (11): 1883-1890.doi: 10.3724/SP.J.1006.2010.01883

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

赤霉素诱导甘蔗节间伸长基因的cDNA-SCoT差异表达分析

吴建明1, 2, 3,李杨瑞2,3, 4,*,王爱勤3,杨柳2, 3,杨丽涛3   

  1. 1 广西农业科学院经济作物研究所，广西南宁 530004；2 广西作物遗传改良生物技术重点开发实验室，广西南宁 530007；3广西大学广西亚热带生物资源保护利用重点实验室，广西南宁 530007；4中国农业科学院甘蔗研究中心，广西南宁 530007
  • 收稿日期:2010-01-04 修回日期:2010-06-28 出版日期:2010-11-12 网络出版日期:2010-08-30
  • 通讯作者: 李杨瑞, E-mail: liyr@gxaas.net
  • 基金资助:

    本研究由国家科技支撑计划项目(2007BAD30B03)，广西科技攻关重点项目(桂科攻0782004-3)，广西科技攻关项目(桂科能0630006-5F和桂科能0815011)和现代农业产业技术体系建设专项资金(nycytx-024-01-15)资助。

Differential Expression of Genes in Gibberellin-Induced stalk elongation of Sugarcane Analyzed with cDNA-ScoT

WU Jian-Ming1, 2, 3,LI Yang-Rui2, 3, 4 *,WANG Ai-Qin3,YANG Liu2, 3,YANG Li-Tao3   

  1. 1 Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 2 Guangxi Crop Genetic Improvement and Biotechnology Lab, Nanning 530007, China; 3 Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning 530004, China; 4 Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Nanning 530007, China
  • Received:2010-01-04 Revised:2010-06-28 Published:2010-11-12 Published online:2010-08-30
  • Contact: LI Yang-Rui,E-mail:liyr@gxaas.net

PDF

2706

被引次数

50

摘要： 以甘蔗ROC22为材料，在伸长初期进行叶面喷施200 mg L–1浓度的赤霉素为处理，以喷施清水为对照，在不同时间取幼茎样品，用cDNA-SCoT进行基因差异表达分析。结果表明，通过46条引物筛选了约700个cDNA片段，获得差异片段30个。有18个基因片段受赤霉素上调而12个基因片段下调；其中16个TDFs序列和NCBI数据库中已录入的基因具有较高的相似性，按照其功能可分为6类，即能量与代谢相关基因(占总数的20.0%)、未知功能蛋白(占总数的20.0%)、未知基因(占总数的46.7%)、信号传导相关基因(占总数的6.7%)和转录因子相关基因(占总数的3.3%)；细胞凋亡基因(3.3%)。这说明利用SCoT方法可以进行蔗茎基因差异表达研究，分离到的一些差异片段可能是与甘蔗节间伸长相关的基因。

关键词: 甘蔗, cDNA-SCoT, 差异表达, 赤霉素

Abstract: The present study was conducted to investigate the molecular mechanisms of gibberellin-induced stalk internodes elongation in sugarcane. Using sugarcane variety ROC22, the plant leaves were treated with spraying gibberellic acid (GA3) at 200 mg L–1 at early elongation stage, and spraying water as a control. Samples of young stalks were taken and the differentially expressed genes were analyzed with cDNA-SCoT technique. The results showed that 30 fragments of differentially expressed genes were obtained by screening 700 cDNA fragments with 46 single primers. The expressions of 18 genes were up-regulated, and 12 genes down-regulated by GA3 induction, and the sequences of 16 TDFs showed high similarity to the registered genes in the NCBI database. These genes were divided into six categories by functional analysis, including energy and metabolism-related genes (accounting for 20.0%), unknown functional proteins (accounting for 20.0%), unknown genes (accounting for 46.7%), signal transduction-related genes (accounting for 6.7%), transcription factor-related genes (accounting for 3.3%), and cell apoptosis (accounting for 3.3%). This suggested that the differential expression of genes in the stalks can be analyzed with SCoT technique, and some fragments may be related to the stalk elongation of surgarcane.

Key words: Gibberellin, Sugarcane, cDNA-SCoT, Differential expression

[1]Hironori T, Mut Y A, Makoto M. The gibberellin signaling pathway is regulated by the appearance and disappearance of SLENDER RICE1 in nuclei. Plant Cell, 2002, 14: 57–70
[2]Moore P H, Buren L L. Gibberellin studies with sugarcane: I. Cultivar differences in growth responses to gibberellic acid. Crop Sci, 1978, 18: 443–446
[3]Buren L L, Moore P H, Yamasaki Y. Gibberellin studies with sugarcane: II. Hand-sampled field trials. Crop Sci, 1979, 19: 425–428
[4]Most B H, Vlitos A J. Gibberellins of sugarcane. Plant Physioogyl, 1966, 41: 1090–1094
[5]Reid J B, Botwright A N, Smith J J, O’Neill D P, Kerckhoffs L H J. Control of gibberellin levels and gene expression during deetiolation in pea. Plant Physiol, 2002, 123: 734–741
[6]Stavang J A, Lindgard B, Emtsen A, Lid S E, Moe R, Olsen J E. Thermoperiodic stem elongation involves transcriptional regulation of gibberellin deactivation in pea. Plant Physiol, 2005, 138: 2344–2354
[7]Dai M, Zhao Y, Ma Q, Hu Y F, Hedden P, Zhang Q F, Zhou D X. The rice YABBY1 gene is involved in the feedback regulation of gibberellin metabolism. Plant Physioy, 2007, 144: 121–133
[8]Weller J L, Hecht V, Vander Schoor J K, Davidson S E, Ross J J. Light regulation of gibberellin biosynthesis in pea is mediated through the COP1/HY5 pathway. Plant Cell, 2009, 10: 1–14
[9]Collard B C Y, Mackill D J. Start codon targeted (SCoT) polymorphism: A simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Mol Biol Rep, 2009, 27: 86–93
[10]Joshi C, Zhou H, Huang X Q Chiang V L. Context sequences of translation initiation codon in plants. Plant Mol Biol, 1997, 35: 993–1001
[11]Sawant S V, Singh P K, Gupta S K, Madnala R, Tuli R. Conserved nucleotide sequences in highly expressed genes in plants. J Genet, 1999, 78: 123–131
[12]Xiong F-Q(熊发前), Tang R-H(唐荣华), Chen Z-L(陈忠良), Pan L-H(潘玲华), Zhuang W-J(庄伟建). SCOT: a novel gene targeted marker technique based on the translation start codon. Mol Plant Breed (分子植物育种), 2009, 7(3): 635–638 (in Chinese with English abstract)
[13]Giranton J L, Dumas C, Cock J M, Gaude T. The integral membrane S-locus receptor kinase of Brassica has serine/threonine kinase activity in a membranous environment and spontaneously forms oligomers in planta. Proc Natl Acad Sci USA, 2000, 97: 3759–3764
[14]Hajouj T, Michelis R, Gepstein S. Cloning and characterization of a receptor-like protein kinase gene associated with senescence. Plant Physiol, 2000, 124: 1305–1314
[15]Clark S E, Williams R W, Meyerowitz E M. The CLAVATA1 gene encodes a putative receptor kinase that controls shoot and floral meristem size in Arabidopsis. Cell, 1997, 89: 575–585
[16]Sessa G D, Ascenzo M, Loh Y T, Martin G B. Biochemical properties of two protein kinases involved in disease resistance signaling in tomato. J Biol Chem, 1998, 273: 15860–15865
[17]Torii K U. The Arabidopsis ERECTA gene encodes aputative receptor protein kinase with extracellular leucine-rich repeats. Plant Cell, 1996, 8: 735–746
[18]Hong S W, Jon J H, Kwak J M, Nam H G. Identification of a receptor-like protein kinase gene rapidly induced by abscisic acid, dehydration, high salt and cold treatments in Arabidopsis thaliana. Plant Physiol, 1997, 113: 1203–1212
[19]Wu J-M(吴建明), Li Y-R(李杨瑞), Yang L(杨柳), Wang A-Q(王爱勤), Yang L-T(杨丽涛). Relationship between gibberellin-induced internode elongation and endogenous hormone changes in sugarcane. Chin J Trop Crops (热带作物学报), 2009, 30(10): 1452–1456 (in Chinese with English abstract)
[20]Lalty J H, Lee B M, Wright P E. Zinc finger proteins: new insights into structural and functional diversity. Curr Opin Struct Biol, 2001, 11: 39–46
[21]Liu H-M(刘梅芳), Zhou S-F(周淑芬), Xu G-L(徐桂磊), Liu H-Q(刘华清), Wang F(王锋). Expression analysis of a rice CCCH-type zinc finger gene. Fujian J Agric Sci (福建农业学报), 2008, 23: 225–230 (in Chinese with English abstract)
[22]Yue C-W(岳昌武), Xiao J(肖静), Ling X(凌锌), Zeng N(曾霓). Effect of low temperature stress on sweet potato S-adenosyl methionine synthetase gene expression. Agric Sci & Technol (农业科学与技术), 2008, 9(1): 11–14 (in Chinese with English)
[23]Jia L-N(贾丽娜), Zhang H-J(张慧杰), Zhang Y-M(张晏萌), Yang Y-R(杨玉荣), Yu A-L(余爱丽). Cloning and sequence analysis of SAMS gene from Zea mays. J Hebei Agric Sci (河北农业科学), 2008, 12(8): 53–55 (in Chinese with English abstract)
[24]Murase K, Hirano Y, Sun T P, Hakoshima T. Gibberellin-induced DELLA recognition by the gibberellin receptor GID1. Nature, 2008, 456: 459–463
[25]Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Chow T Y, Hsing Y C, Kitano H, Yamaguchi I, Matsuoka M. GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature, 2005, 437: 693–698
[26]Ueguchi-Tanaka M, Nakajima M. Katoh E, Ohmiyaa H, Asanoa K, Sajic S, Hongyuc X, Ashikaria M, Kitanoa H, Yamaguchid I, Matsuoka M. Molecular interactions of a soluble gibberellin receptor, GID1, with a rice DELLA protein, SLR1, and gibberellin. Plant Cell, 2007, 19: 2140–2155
[1] 崔致远, 秦晨展, 刘星雨, 张海, 曾康, 黄国强, 徐景升. 甘蔗类四跨膜蛋白ScTSPAN18与6K2互作应答SCMV侵染研究[J]. 作物学报, 2026, 52(6): 1618-1630.
[2] 田春艳, 陆鑫, 吴才文, 徐超华, 刘家勇, 边芯, 桃联安. 基于荧光SSR的甘蔗创新种质遗传多样性分析及育种潜力评估[J]. 作物学报, 2026, 52(4): 1057-1072.
[3] 杨宗桃, 杨婷, 王禹童, 艾静, 李燕烨, 刘家勇, 邓军, 赵勇, 张跃彬. 甘蔗CLC基因家族鉴定与表达分析[J]. 作物学报, 2026, 52(3): 722-734.
[4] 张宇, 刘芳, 蔡诚诚, 杨小华, 吉阿么石扎, 杨元军, 王西瑶. 溴乙烷与赤霉素协同处理破除马铃薯块茎休眠的机理初探[J]. 作物学报, 2026, 52(3): 825-838.
[5] 刘迪, 黎瑞源, 石茂竹, 李洪有, 陈庆富, 石桃雄. 苦荞半矮秆突变体sd3的表型鉴定及转录组分析[J]. 作物学报, 2026, 52(1): 316-328.
[6] 万慧兰, 吴华英, 曾丹, 钱禛锋, 赵昌祖, 廖然超, 何丽莲, 李富生. 蔗茅耐寒相关基因EfWRKY51克隆分析及功能验证[J]. 作物学报, 2025, 51(8): 2048-2059.
[7] 王青, 王伊秀, 李越男, 吕永辉, 张海波, 刘娜, 程红艳. 高、低Cd积累小麦对Cd胁迫的转录组学响应差异[J]. 作物学报, 2025, 51(5): 1230-1247.
[8] 张金泽, 周庆国, 杨旭, 王倩, 肖莉晶, 金海润, 欧阳青静, 余坤江, 田恩堂. 芥菜型油菜响应菌核病侵染表达特性与高抗性关联基因分析[J]. 作物学报, 2025, 51(3): 621-631.
[9] 许睿, 何妙华, 王昊, 李卫, 任杰, 夏志强. 基于空间转录组技术解析大豆种胚对X射线辐射的响应机制[J]. 作物学报, 2025, 51(12): 3121-3132.
[10] 桂灵星, 凌溪铁, 唐兆成, 罗文臻, 朱盼珍, 仇泽宇, 张保龙. 宁麦36抗甲氧咪草烟矮秆突变体的鉴定及农艺性状分析[J]. 作物学报, 2025, 51(11): 2923-2932.
[11] 赵慧霞, 郭彦丽, 郑渝泠, 何棱, 陈锐, 王珊珊, 曾长立, 邹珺, 沈金雄, 傅廷栋, 刘小云, 万何平. 甘蓝型油菜响应碱胁迫的基因表达差异分析[J]. 作物学报, 2025, 51(11): 3105-3118.
[12] 匡博文, 韦妳, 刘金典, 陈美燕, 毛兴洁, 段维兴, 杨细平. 基于甘蔗及其近缘属参考基因组开发SSR标记及数据库[J]. 作物学报, 2025, 51(1): 103-116.
[13] 李嘉欣, 黄莹, 吴潞梅, 赵伦, 易斌, 马朝芝, 涂金星, 沈金雄, 傅廷栋, 文静. 甘蓝型油菜BnaSLY1基因进化分析及功能研究[J]. 作物学报, 2025, 51(1): 44-57.
[14] 李旭娟, 李纯佳, 田春艳, 孔春艳, 徐超华, 刘新龙. 甘蔗硝酸盐转运蛋白1/肽转运蛋白家族6.4基因(ScNPF6.4)克隆及其调控分蘖功能分析[J]. 作物学报, 2024, 50(8): 2131-2142.
[15] 玉泉馨, 杨宗桃, 张海, 程光远, 焦文迪, 曾康, 罗廷绪, 黄国强, 王璐, 徐景升. 甘蔗类钙调素ScCML13与SCMV运动蛋白P3N-PIPO的互作研究[J]. 作物学报, 2024, 50(7): 1855-1866.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2