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作物学报 ›› 2011, Vol. 37 ›› Issue (09): 1551-1558.doi: 10.3724/SP.J.1006.2011.01551

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

陆地棉质体转录活性因子基因GhPTAC的克隆及其耐盐性分析

周凯，叶武威*，王俊娟，王德龙，樊保香，王帅   

  1. 中国农业科学院棉花研究所 / 棉花生物学国家重点实验室 / 农业部棉花遗传改良重点实验室，河南安阳 455000
  • 收稿日期:2011-02-28 修回日期:2011-05-20 出版日期:2011-09-12 网络出版日期:2011-06-28
  • 通讯作者: 叶武威, E-mail: yeww@cricaas.com.cn
  • 基金资助:

    本研究由国家转基因生物新品种培育科技重大专项(2008ZX08005-004)和国家“十一五”科技支撑计划项目(2006BAD13B04-1)资助。

Cloning and Salt-tolerance Analysis of Gene Plastid Transcriptionally Active (GhPTAC) from Gossypium hirsutum L.

ZHOU Kai,YE Wu-Wei*,WANG Jun-Juan,WANG De-Long,FAN Bao-Xiang,WANG Shuai   

  1. Cotton Research Institute, Chinese Academy of Agricultural Science / State Key Laboratory of Cotton Biology / Key Laboratory of Cotton Genetic Improvement of Agriculture Ministry, Anyang 455000, China
  • Received:2011-02-28 Revised:2011-05-20 Published:2011-09-12 Published online:2011-06-28
  • Contact: 叶武威, E-mail: yeww@cricaas.com.cn

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摘要： 为了挖掘新的耐盐基因及调控途径，利用基因芯片技术及抑制性差减文库技术筛选到质体转录活性因子，通过RACE及RT-PCR技术克隆到该基因的cDNA全长，命名为GhPTAC。该cDNA全长1 564 bp，其中ORF 1 038 bp，推测编码345个氨基酸残基的多肽。生物信息学分析表明GhPTAC为拟南芥PTAC13同源基因，同源性60.6%。编码蛋白为转录活跃的染色体(TAC)的一个组分，参与叶绿体基因组转录终止/抗终止调节。Real-time PCR分析结果表明，GhPTAC受盐胁迫诱导上调表达，在耐盐材料中9806中表达水平明显高于盐敏感材料中S9612，这与芯片结果一致。

关键词: 陆地棉, 盐胁迫, PTAC, Real-time PCR

Abstract: To develop novel salt-tolerance genes and adjustment pathway, we screened out gene Plastid Transcriptionally Active named as GhPTAC based on salt-tolerance gene chips and salt resistance related SSH library.The result of RACE and RT-PCR showed that the cDNA full length was 1 564 bp, and ORF was 1 038 bp, which encoded 345 amino acid residues. Bioinformatics analysis showed that GhPTAC shared the identity of 60.6% with the homologous gene PTAC13 from Arabidopsis thaliana. As one part of Transcriptionally active chromosome (TAC), GhPTAC plays a part role in regulation of transcription termination/ antitermination of chloroplastid genome. The GhPTAC expression was up-regulated under salt stress induction and the expression level of GhPTAC of Zhong 9806 (salt-resistant material) was obviously higher than that Zhong S9612 (salt-sensitive material) which was measured by Real-time PCR and in accord with the arrays results.

Key words: Gossypium hirsutum, Salt stress, PTAC, Real-time PCR

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