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作物学报 ›› 2012, Vol. 38 ›› Issue (09): 1570-1582.doi: 10.3724/SP.J.1006.2012.01570

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆NF-YB家族全基因组鉴定、分类和表达

郑炜君1,2，徐兆师2,*，冯志娟2，李连城2，陈明2，柴守诚1，马有志2   

  1. 1西北农林科技大学农学院，陕西杨凌 712100； 2中国农业科学院作物科学研究所 / 国家农作物基因资源与基因改良重大科学工程 / 农业部作物遗传育种重点开放实验室，北京 100081
  • 收稿日期:2012-02-17 修回日期:2012-04-16 出版日期:2012-09-12 网络出版日期:2012-07-03
  • 通讯作者: 徐兆师, E-mail: xuzhaoshi@yahoo.com.cn, Tel: 010-82106773; 柴守诚, E-mail: chaishoucheng@126.com, Tel: 029-87082205
  • 基金资助:

    本研究由国家自然科学基金项目(31171546)和转基因生物新品种培育科技重大专项(2011ZX08002-002, 2009ZX08009-083B)资助。

Genome-Wide Identification, Classification, and Expression of NF-YB Gene Family in Soybean

ZHENG Wei-Jun1,2,XU Zhao-Shi2,*,FENG Zhi-Juan2,LI Lian-Cheng2,CHEN Ming2,CHAI Shou-Cheng1,MA You-Zhi2   

  1. 1 College of Agronomy, Northwest A & F University, Yangling 712100, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture, Beijing 100081, China
  • Received:2012-02-17 Revised:2012-04-16 Published:2012-09-12 Published online:2012-07-03
  • Contact: 徐兆师, E-mail: xuzhaoshi@yahoo.com.cn, Tel: 010-82106773; 柴守诚, E-mail: chaishoucheng@126.com, Tel: 029-87082205

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摘要： 利用大豆基因组数据库，通过生物信息学手段，鉴定大豆NF-YB家族基因的全序列、定位和拷贝数，通过序列比对进行进化和分类分析。利用大豆高通量RNA测序数据、NCBI中UniGene的EST表达数据进行组织表达谱分析。结果表明，大豆基因组中含有28个NF-YB家族基因，分布于大豆的14条染色体上，系统进化树分析将其分成3类。启动子分析表明，几乎全部NF-YB家族基因的启动子区均含有逆境应答反应顺式作用元件。各个发育阶段中，多数成员至少在一个组织中表达，10个差异表达的基因中有2个在根瘤中特异表达，2个在根中特异表达，2个在根瘤中优势表达，另外4个在其他部位优势表达。研究结果促进了NF-YB家族基因的功能研究与利用。

关键词: NF-YB家族, 全基因组鉴定, 进化, 差异表达, 启动子

Abstract: Based on soybean genome database and bioinformatics method, we obtained soybean NF-YB family genes and their positions on chromosome and duplication information. NF-YB proteins were classified according to their phylogenetic relationship. The soybean RNA-sequencing data from the SoyBase database and EST data from NCBI UniGene were used to analyze the expression pattern of these genes at different development stages. A total of 28 NF-YB genes were systematically identified from soybean and classified into three types. They were located on 14 chromosomes and most of them had stress related cis-acting elements in their promoter regions. Ten differentially expressed genes were found at each developmental stage, and four of them were highly expressed in different tissues other than root nodules and root. Among other six genes, two were highly expressed in root nodule, two were specifically expressed in root nodules and two were specifically expressed in root. The results facilitate functional analysis and utilization of NF-YB genes in crop genetic improvement.

Key words: NF-YB, Genome-wide identification, Phylogeny, Differential expression, Promoter

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