作物学报 ›› 2012, Vol. 38 ›› Issue (10): 1875-1883.doi: 10.3724/SP.J.1006.2012.01875
王后苗,黄家权,雷永,晏立英,王圣玉,姜慧芳,任小平,娄庆任,廖伯寿*
WANG Hou-Miao,HUANG Jia-Quan,LEI Yong,YAN Li-Ying,WANG Sheng-Yu,JIANG Hui-Fang,REN Xiao-Ping,LOU Qing-Ren, LIAO Bo-Shou*
摘要:
选用抗黄曲霉产毒的花生品种(系)和高产毒品种(系), 采用强产毒菌株AF2202人工接种水分吸涨的花生种子, 培养7 d后,测定接种和未接种的花生种子中白藜芦醇及黄曲霉毒素含量,探讨白藜芦醇与花生种子黄曲霉产毒抗性之间的关系。结果表明,抗黄曲霉产毒花生品种(系)的白藜芦醇含量较高,平均为37.3 µg kg–1,高产毒品种含量相对较低,平均为13.3 µg kg–1,抗、感品种之间存在显著差异。吸胀处理后抗产毒花生品种(系)白藜芦醇含量提高2.0倍,感病品种(系)仅提高1.6倍,对不同品种(系)处理后的种子二次接种,令黄曲霉毒素含量下降37.6%~75.8%,但吸胀处理后抗产毒品种(系)的黄曲霉毒素含量仍低于感病品种(系)。相关分析表明,花生白藜芦醇含量与黄曲霉毒素含量呈显著负相关,并且在离体培养基中添加浓度大于3.0 μg mL–1的白藜芦醇可导致黄曲霉菌产毒量大幅下降,说明白藜芦醇对黄曲霉产毒具有抑制作用。
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