作物学报 ›› 2013, Vol. 39 ›› Issue (09): 1576-1581.doi: 10.3724/SP.J.1006.2013.01576
杨丽华1,2,王金凤2,杜丽璞2,徐惠君2,魏学宁2,李钊2,马翎健1,*,张增艳2,*
YANG Li-Hua1,2,WANG Jin-Feng2,DU Li-Pu2,XU Hui-Jun2,WEI Xue-Ning2,LI Zhao2,MA Ling-Jian1,*,ZHANG Zeng-Yan2,*
摘要:
全蚀病和根腐病是小麦(Triticum aestivum)重要的土传真菌病害。PgPGIP1是人参(Panax ginseng)的一种多聚半乳糖醛酸酶抑制蛋白,可以抑制部分病原真菌分泌的多聚半乳糖醛酸酶的活性。本研究人工合成了PgPGIP1基因,并构建PgPGIP1基因的单子叶植物表达载体pA25-PgPGIP1,通过基因枪介导法将其转入小麦品种扬麦18中。对转PgPGIP1基因的T0至T4代植株进行PCR、RT-PCR和Q-RT-PCR分析,并对其全蚀病和根腐病抗性进行鉴定。结果表明,PgPGIP1基因能够在4个转基因小麦株系中遗传、转录与表达。与未转基因的小麦扬麦18相比,4个转基因小麦株系对全蚀病与根腐病的抗性明显提高,说明PgPGIP1表达增强了转基因小麦对全蚀病与根腐病的抗性。
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