作物学报 ›› 2014, Vol. 40 ›› Issue (03): 397-404.doi: 10.3724/SP.J.1006.2014.00397
肖炳光1,*, 邱杰2,曹培健3,桂毅杰2,卢秀萍1,李永平1,樊龙江2
XIAO Bing-Guang1,*,QIU Jie2,CAO Pei-Jian3,GUI Yi-Jie2,LU Xiu-Ping1,LI Yong-Ping1,FAN Long-Jiang2
摘要:
提出了一种基于基因组简约法开发SNP标记的方法, 即利用特定限制性内切酶酶切降低基因组复杂度, 利用高通量测序平台对酶切位点周围的目标片段进行富集测序, 设计一个生物信息学流程进行序列分析和SNP鉴定。以烤烟DH群体为例, 通过基因组简约法收集烟草基因组代表性片段和高通量测序产生11.4 Gb数据, 经生物信息学分析获得了1015个高质量SNP位点。以SSR标记为骨架, 绘制包括SNP标记在内、标记总数为1307的烤烟遗传连锁图。最后利用该遗传图谱和普通烟草2个祖先种的基因组序列, 分析烟草24个连锁群(染色体)之间的同源关系, 发现了大量染色体之间的重组或交换事件以及部分染色体之间的共线性。
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