洋葱63A细胞质雄性不育与绒毡层的提早衰退有关

作物学报 ›› 2006, Vol. 32 ›› Issue (03): 369-372.

洋葱63A细胞质雄性不育与绒毡层的提早衰退有关

李园园;杨清;严继勇;陈敏

南京农业大学生命科学学院, 江苏南京210095

收稿日期:2005-02-25 修回日期:1900-01-01 出版日期:2006-03-12 网络出版日期:2006-03-12
通讯作者: 杨清

Cytoplasmic Male-Sterility of Onion (Allium cepa L.) 63A Is Associated with Premature Degeneration of the Tapetum

LI Yuan-Yuan; YANG Qing; YAN Ji-Yong and CHEN Min

College of Life Sciences, Nanjing Agricutural University, Nanjing 210095, Jiangsu

Received:2005-02-25 Revised:1900-01-01 Published:2006-03-12 Published online:2006-03-12
Contact: YANG Qing

摘要/Abstract

摘要：

细胞质雄性不育（CMS）机理的细胞学研究在许多作物上已有报道，如拟南芥、大豆、水稻、小麦等，但在国内至今未见关于洋葱的此类报道。利用光镜和透射电镜对洋葱不育系63A和保持系63B进行花药发育过程中显微结构和超微结构观察，结果表明，不育花药花粉母细胞时期，花粉母细胞发育正常，花粉囊形状不规则，绒毡层发育迟缓。四分体时期，四分体形成正常，中层严重退化，绒毡层与药室壁完全脱离，细胞质浓缩、空泡化。利用DNA梯度技术发现不育系绒毡层细胞提早发生程序性死亡。小孢子发育时期，小孢子细胞质发生浓缩、降解，绒毡层完全解体。推测不育系小孢子败育与中层、绒毡层提前衰退有关。

关键词: 洋葱, 败育, 细胞质雄性不育, 细胞学, 小孢子

Abstract:

Cytological studies of cytoplasmic male-sterility (CMS) mechanism have been reported in several crops of Arabidopsis, soybean, rice, wheat etc. To date no report on CMS studies in onion (Allium cepa L.) is available in China. By using the technology of light microscopy and transmission electron microscopy, microstructural and ultrastructural observations of male-sterility line 63A and maintainer line 63B anthers were done during their development in onion (Allium cepa L.). The results indicated that in the male sterile anther, pollen sacs were formed irregularly and the tapetum formation was incomplete at the pollen-mother cell stage. The middle layer was degenerated completely and collapsed into a thin layer, the tapetal cells become vacuolated and dissociated from the pollen sac wall, followed by condensation of the tapetal cytoplasm at the tetrad stage. Meanwhile, it was presumed that the tapetal cells in male sterile anther underwent premature programmed cell death (PCD) based on the DNA ladder. At the developing microspore stage, the complete disintegration of cytoplasm in the microspore was observed. It is suggested that the microspore abortion in male-sterile anther would be attributed to premature degeneration of middle layer and tapetum.

Key words: Allium cepa L., Abortion, Cytoloplasmic male-sterility, Cytology, Microspores

中图分类号:

S633

李园园;杨清;严继勇;陈敏. 洋葱63A细胞质雄性不育与绒毡层的提早衰退有关[J]. 作物学报, 2006, 32(03): 369-372.

LI Yuan-Yuan; YANG Qing; YAN Ji-Yong and CHEN Min. Cytoplasmic Male-Sterility of Onion (Allium cepa L.) 63A Is Associated with Premature Degeneration of the Tapetum[J]. Acta Agron Sin, 2006, 32(03): 369-372.

参考文献

相关文章 15

[1]	吴然然, 林云, 陈景斌, 薛晨晨, 袁星星, 闫强, 高营, 李灵慧, 张勤雪, 陈新. 绿豆雄性不育突变体msm2015-1的遗传学与细胞学分析[J]. 作物学报, 2021, 47(5): 860-868.
[2]	唐鑫, 李圆圆, 陆俊杏, 张涛. 甘蓝型油菜温敏细胞核雄性不育系160S花药败育的形态学特征和细胞学研究[J]. 作物学报, 2021, 47(5): 983-990.
[3]	陈淼, 谢赛, 王超智, 李焱龙, 张献龙, 闵玲. 棉花GhPIF4调控高温下花药败育机制初探[J]. 作物学报, 2020, 46(9): 1368-1379.
[4]	蒋会兵,杨盛美,刘玉飞,田易萍,孙云南,陈林波,唐一春. 厚轴茶雄性不育株花药败育的生物学特性和细胞学研究[J]. 作物学报, 2020, 46(7): 1076-1086.
[5]	殷家明,钟荣棋,林呐,唐章林,李加纳. 诸葛菜小孢子培养及其单倍体减数分裂染色体配对观察[J]. 作物学报, 2020, 46(02): 194-203.
[6]	牟碧涛,赵卓凡,岳灵,李川,张钧,李章波,申汉,曹墨菊. 两份玉米CMS-C恢复系的育性恢复力测定及恢复基因的分子标记定位[J]. 作物学报, 2019, 45(2): 225-234.
[7]	陶瑶，王瑜，钟思荣，吴凌敏，谢丽娟，聂亚平，周玮，王建革，刘齐元. 烟草ATP合酶F₀部分4个亚基基因转录本编辑位点分析[J]. 作物学报, 2016, 42(12): 1743-1753.
[8]	王鑫,马莹雪,杨阳,王丹峰,殷慧娟,王洪刚. 小麦矮秆种质SN224的鉴定及农艺性状QTL分析[J]. 作物学报, 2016, 42(08): 1134-1142.
[9]	杨坤,刘欣,杜丽璞,叶兴国,张增艳. 转AcAMP-sn基因抗全蚀病小麦新种质的创制与鉴定[J]. 作物学报, 2014, 40(01): 22-28.
[10]	李双双,刘国政,陈志文,王玉美,华金平. 哈克尼西棉细胞质陆地棉雄性不育系orf160的克隆及遗传转化[J]. 作物学报, 2013, 39(09): 1538-1547.
[11]	孟佳佳,董树亭,石德杨,张海燕. 玉米雌穗分化与籽粒发育及败育的关系[J]. 作物学报, 2013, 39(05): 912-918.
[12]	祁建民，马红勃，徐建堂，陈美霞，周东新，王涛，陈顺辉. 烟草细胞质雄性不育系及其保持系的花蕾差异蛋白质分析[J]. 作物学报, 2012, 38(07): 1232-1239.
[13]	毕惠惠, 杨华瑞, 马俊会, 刘录祥, 王成社, 许喜堂, 邹淑芳, 谢彦周. 陕农138小孢子发育过程中蛋白表达差异的比较分析[J]. 作物学报, 2012, 38(03): 462-470.
[14]	葛娟, 郭英芬, 于澄宇, 张国云, 董军刚, 董振生. 甘蓝型油菜光、温敏雄性不育系Huiyou50S花粉败育的细胞学观察[J]. 作物学报, 2012, 38(03): 541-548.
[15]	冯汉宇, 王志敏, 孔凡娜, 张敏洁, 周顺利. 基于控制授粉技术的玉米籽粒生育特性与建成机制研究[J]. 作物学报, 2011, 37(09): 1605-1615.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed