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作物学报 ›› 2012, Vol. 38 ›› Issue (03): 541-548.doi: 10.3724/SP.J.1006.2012.00541

• 研究简报 • 上一篇    下一篇

甘蓝型油菜光、温敏雄性不育系Huiyou50S花粉败育的细胞学观察

葛娟1，郭英芬1，于澄宇1,*，张国云2，董军刚1，董振生1,*   

  1. 1西北农林科技大学农学院，陕西杨凌712100; 2西北农林科技大学大型仪器设备管理中心，陕西杨凌712100
  • 收稿日期:2011-09-05 修回日期:2011-12-15 出版日期:2012-03-12 网络出版日期:2012-01-04
  • 通讯作者: 于澄宇, E-mail:yu1009@nwsuaf.edu.cn; 董振生, E-mail:dzs05319@163.com
  • 基金资助:

    本研究由国家自然科学基金(31071454), 教育部新世纪优秀人才项目(NCET-10-0693)和中央高校基本科研业务费专项(QN2009008)资助。

Cytological Observation of Anther Development of Photoperiod/Thermo- sensitive Male Sterile Line Huiyou50S in Brassica napus

GE Juan1,GUO Ying-Fen1,YU Cheng-Yu1,*,ZHANG Guo-Yun2,DONG Jun-Gang1,DONG Zhen-Sheng1,*   

  1. 1 College of Agronomy, Northwest A&F University, Yangling 712100, China; 2 Management centre for large equipment, Northwest A&F University, Yangling 712100, China
  • Received:2011-09-05 Revised:2011-12-15 Published:2012-03-12 Published online:2012-01-04
  • Contact: 于澄宇, E-mail:yu1009@nwsuaf.edu.cn; 董振生, E-mail:dzs05319@163.com

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被引次数

18

摘要：  Huiyou50S是从甘蓝型油菜品种汇油50中发现的半不育株选育而成的光、温敏雄性不育系，育性受隐性核基因控制，在高温、长光周期下不育而在低温、短光周期条件下可育。本文通过半薄树脂切片、扫描电镜、花粉压片染色、花药整体透明方法对Huiyou50S及其近等基因系Huiyou50F的花药发育过程及花粉形态观察比较。结果表明, Huiyou50S的花药造孢细胞、花粉母细胞、减数分裂、四分体阶段均正常，在单核期出现明显异常，虽然能形成花粉壁，但小孢子细胞质收缩、解体，最终只剩余空瘪的花粉壳; Huiyou50S的花药绒毡层在单核后期提前降解，绒毡层解体速度快于可育株Huiyou50F。Huiyou50S的小孢子的完全败育主要发生在单核时期，绒毡层发育与小孢子异常有某种关联，该结论为油菜光、温敏雄性不育类型划分及育种应用提供了依据。

关键词: 甘蓝型油菜, 光、温敏雄性不育, 花粉败育, 小孢子, 细胞学

Abstract: A photoperiod/thermo-sensitive male sterile line, named Huiyou50S, was developed from a spontaneous semi-sterile plant found in Brassica napus cultivar Huiyou50. The male sterility of Huiyou50S was controlled by recessive nuclear genes. Huiyou50S presents male sterile under high temperature and long photoperiod condition, and fertility under low temperature and short photoperiod. The cytological characters of pollen and the anther development of Huiyou50S at the different stages were compared with its near isogenic line Huiyou50F, which is the male fertile sibling, by techniques including semi-thin sections, scan electronic microscopy, pollen grains staining with aceto-carmine, and whole anther clearing with methyl salicylate. The result indicated that the anther development of Huiyou50S was similar to that of Huiyou50F from sporogenous cell, pollen mother cell, meiosis, to tetrad stage. At the uni-nucleate stage, although the exine formed, the protoplast of microspores condensed, and the cell became empty. At last all cellular content of pollen degraded completely and only the empty and distorted shell remained. The tapetum development of Huiyou50S was similar to that of Huiyou50F up to tetrad stage, but its tapetal cells rapidly disintegrated at the uni-nucleate stage, which occurred earlier than that in Huiyou50F, while the tapetum of Huiyou50F did not degenerate at this stage. In conclusion, the microspores of Huiyou50S abort mainly at the uni-nucleate stage, and there are some associations between pollen abortion and tapetal cells’ disintegration. This result is helpful to classification and utility of photoperiod/thermo-sensitive genic male sterility

Key words: Brassica napus, Photoperiod/thermo sensitive genic male, sterility, Pollen abortion, Microspore, Cytology

[1]Yu C-Y(于澄宇). Progress and perspective of different pollination control systems in rapeseed (Brassica napus). J Northwest A&F Univ (Nat Sci Edn) (西北农林科技大学学报?自然科学版), 2011, 39(9): 1–8 (in Chinese with English abstract)

[2]Xi D-W(席代汶), Chen W-J(陈卫江), Ning Z-L(宁祖良). Breeding of a thermo-sensitive genic male-sterile line Xiang 91S in Brassica napus L. Hunan Agric Sci (湖南农业科学), 1994, (4): 17–18 (in Chinese with English abstract)

[3]Wang H(王华), Tang X-H(汤晓华), Zhao J-X(赵继献). Genetic study on ecotype genetic male sterile of H90S in Brassica napus L. Chin J Oil Crop Sci (中国油料作物学报), 2001, 23(4): 11–15 (in Chinese with English abstract)

[4]Liu Z-W(刘尊文), Wu P(吴平), Yuan W-H(袁卫红), Zhou J-G(周贱根), Zhou X-P(周小萍). Breeding of a photo-and-temperature sensitive genic male-sterile dual-use line N196S in Brassica napus L. Acta Agric Univ Jiangxiensis (江西农业大学学报), 2006, 28(5): 654–658 (in Chinese with English abstract)

[5]Yu C-Y(于澄宇), Li W(李玮), Chang J-J(常建军), Hu S-W(胡胜武). Development of a thermo-sensitive male-sterile line 373S in Brassica napus L. Chin Agric Sci Bull (中国农学通报), 2007, 23(7): 245–248 (in Chinese with English abstract)

[6]Xi D-W(席代汶), Wu X-M(邬贤梦), Ning Z-L(宁祖良), Deng X-X(邓锡兴), Chen W-J(陈卫江), Yi D-L(易冬莲), Li M(李莓), Huang H-L(黄虎兰), Ding D-J(丁登杰). Breeding of a two-line hybrid variety Xiang Zayou 5 with double-low quality in Brassica napus L. Chin J Oil Crop Sci (中国油料作物学报), 2005, 27(1): 23–25 (in Chinese with English abstract)

[7]Dong J-G(董军刚), Dong Z-S(董振生), Liu X-X(刘绚霞), Liu C-S(刘创社), Li H-B(李红兵). Cytological studies on anther development of ecological male sterile line 533S in Brassica napus L. J Northwest Sci-Tech Univ Agric Fore (西北农林科技大学学报), 2004, 32(7): 61–66 (in Chinese with English abstract)

[8]Sun X-M(孙晓敏), Li W(李玮), Li Y(李英), Feng Z-F(冯志峰), Li Y-M(李艳明), Xi G-Q(习广清), Shen G-P(谌国鹏), Hu S-W(胡胜武). Electronic microscope observations on microsporogenesis of ecol-sensitive male sterile line 373S in Brassica napus. Chin Agric Sci Bull (中国农学通报), 2011, 27(7): 123–132 (in Chinese with English abstract)

[9]Sun X-M(孙晓敏), Hu S-W(胡胜武), Yu C-Y(于澄宇). Cytological Observation of anther development of an ecological male sterile line H50S in Brassica napus L. Acta Agric Boreali-occident Sin (西北农业学报), 2009, 18(5): 153–158 (in Chinese with English abstract)

[10]Yu F-Q(余凤群), Fu T-D(傅廷栋). Cytomorphological research on anther development of several male-sterile lines in Brassica napus L. J Wuhan Bot Res (武汉植物学研究), 1990, 8(3): 209–216 (in Chinese with English abstract)

[11]Yang G-S(杨光圣), Qu B(瞿波), Fu T-D(傅廷栋). Cytological study of microsporogenesis in three recessive genic male sterile lines of Brassica napus L. J Huazhong Agric Univ (华中农业大学学报), 1999, 18(6): 520–523 (in Chinese with English abstract)

[12]Long H(龙欢), Yao J-L(姚家玲), Tu J-X(涂金星). Cytological study on anther development in three types of Brassica napus L. male sterility line. J Huazhong Agric Univ (华中农业大学学报), 2005, 24(6): 570–575 (in Chinese with English abstract)

[13]Grant I, Beversdorf W D, Peterson R L. A comparative light and electron microscopic study of microspore and tapetal development in male fertile and cytoplasmic male sterile oilseed rape (Brassica napus). Canadian J Bot, 1986, 64: 1055–1086

[14]Wan Z J, Jing B, Tu J X, Ma C Z, Shen J X, Yi B, Wen J, Huang T, Wang X J, Fu T D. Genetic characterization of a new cytoplasmic male sterility system (hau) in Brassica juncea and its transfer to B.napus. Theor Appl Genet, 2008, 116: 355–362

[15]Pablo Gonza´lez-Melendi, Magalie Uyttewaal, César N. Morcillo, Jose´ Ramo’n Herna´ndez Mora, Susana Fajardo, Francxoise Budar and M. Mercedes Lucas. A light and electron microscopy analysis of the events leading to male sterility in Ogu-INRA CMS of rapeseed (Brassica napus). J Exp Bot, 2008, 59: 827–838

[16]Wei W-L(危文亮), Wang H-Z(王汉中), Liu G-H(刘贵华). Anatomical observations of anther development of NCa, a cytoplasmic male sterile line in rapeseed (Brassica napus L.). Sci Agric Sin (中国农业科学), 2005, 38(6): 1232–1237 (in Chinese with English abstract)

[17]Laser K D, Lersten N K. Anatomy and cytology of microsporogenesis in cytoplasmic male sterile (CMS) angiosperms. Bot Rev, 1972, 38: 425–454

[18]Sanders P M, Bui A Q, Weterings K, McIntire K N, Hsu Y C, Lee P Y, Truong M T, Beals T P, Goldberg R B. Anther developmental defects in Arabidopsis thaliana male-sterile mutants. Sex Plant Reprod, 1999, 11: 297–322

[19]Glover J, Grelon M, Craig S, Chaudhury A, Dennis E. Cloning and characterization of MS5 from Arabidopsis: a gene critical in male meiosis. Plant J, 1998, 15: 345–356

[20]Xie C-T(谢潮添), Wei D-M(魏冬梅), Tian H-Q(田惠桥). Advances in cell biological researches on male sterility of higher plants. J Plant Physio Mol Biol (植物生理与分子生物学学报), 2006, 32(1): 17–23 (in Chinese with English abstract)

[21]Yi B, Zeng F Q, Lei S, Chen Y, Yao X, Zhu Y, Wen J, Shen J, Ma C, Tu J, Fu T D. Two duplicate CYP704B1-homologous genes BnMs1 and BnMs2 are required for pollen exine formation and tapetal development in Brassica napus. Plant J, 2010, 63: 925–938

[22]Chen W W, Yu X H, Zhang K S, Shi J X, De Oliveira S, Schreiber L, Shanklin J, Zhang D B. Male Sterile2 encodes a plastid-localized Fatty Acyl Carrier Protein Reductase required for pollen exine development in Arabidopsis. Plant Physiol, 2011, 157: 842–853

[23]Pacini E. Tapetum character states: analytical keys for tapetum types and activities. Canad J Bot, 1997, 75: 1448–1459

[24]Vizcay-Barrena G, Wilson Z A. Altered tapetal PCD and pollen wall development in the Arabidopsis ms1 mutant. J Exp Bot, 2006, 57: 2709–2717

[25]Scoles G J, Evans L E. Pollen development in male fertile and cytoplasmic male sterile rye. Canadian J Bot, 1979, 57: 2782–2790

[26]Xie C-T(谢潮添), Yang Y-H(杨延红), Ge L-L(葛丽丽), Wang R(王瑞), Tian H-Q(田惠桥). The ultrastructural observation of anthers of Chinese cabbage’s male-sterility. Acta Biol Exp Sin (实验生物学报), 2005, 38(6): 501–512 (in Chinese with English abstract)
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