欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2022, Vol. 48 ›› Issue (3): 759-769.doi: 10.3724/SP.J.1006.2022.14025

• 研究简报 • 上一篇    下一篇

甘蓝型油菜白花基因InDel连锁标记开发

王瑞1,2(), 陈雪1,2, 郭青青1,2, 周蓉1,2, 陈蕾1,2, 李加纳1,2,*()   

  1. 1西南大学农学与生物科技学院, 重庆 400715
    2重庆市油菜工程技术研究中心, 重庆 400715
  • 收稿日期:2021-02-08 接受日期:2021-07-12 出版日期:2022-03-12 网络出版日期:2021-08-09
  • 通讯作者: 李加纳
  • 作者简介:E-mail: Ruiwang71@163.com
  • 基金资助:
    高等学校学科创新引智计划(111计划)项目(B12006)

Development of linkage InDel markers of the white petal gene based on whole-genome re-sequencing data in Brassica napus L.

WANG Rui1,2(), CHEN Xue1,2, GUO Qing-Qing1,2, ZHOU Rong1,2, CHEN Lei1,2, LI Jia-Na1,2,*()   

  1. 1College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
    2Chongqing Engineering Research Center for Rapeseed, Chongqing 400715, China
  • Received:2021-02-08 Accepted:2021-07-12 Published:2022-03-12 Published online:2021-08-09
  • Contact: LI Jia-Na
  • Supported by:
    Project of Intellectual Base for Discipline Innovation in Colleges and Universities (111 Program)(B12006)

RichHTML

11

PDF

1095

摘要：

碱基插入/缺失(InDel)是基因组上广泛分布的遗传变异形式。但甘蓝型油菜白花基因InDel连锁标记还未见有关研究报道。本研究以甘蓝型油菜双单倍体(doubled haploid, DH)纯系黄花Y05和甘蓝型油菜纯系白花W01杂交构建F2群体。在F2群体中选取30株极端纯白花和30株极端纯黄花构建叶片DNA子代池, 对亲本和DNA子代池进行30×重测序。以法国甘蓝型油菜Darmor-bzh为参考序列, QTL-seq流程和PoPoolation2流程相互结合鉴定白花基因候选区间, 2种方法均将白花基因定位于法国甘蓝型油菜Darmor-bzh C03染色体52~54 Mb区间。利用IGV软件可视化白花基因候选区间插入缺失(InDel)变异位点, 依据候选区间序列信息设计InDel引物, 聚丙烯酰胺凝胶电泳筛选到8个与白花基因连锁共分离的InDel标记。上述研究为甘蓝型油菜白花基因精细定位和分子标记辅助选育以及白花基因功能标记开发奠定了研究基础和工作思路。

关键词: 甘蓝型油菜, 重测序, 白花基因, InDel标记

Abstract:

InDel is widely distributed across the genome and occurs in a high density and large numbers in a genome. To date, the researches about linkage InDel markers of the white petal gene in B. napus L are very less yet. In this study, we constructed the F2 mapping population from the cross between DH Y05 (yellow petal) and DH W01 (white petal). Two bulks with 30 yellow petal lines and 30 white petal lines of F2 population were constructed by mixing an equal amount of DNA. Then two bulks and parents were performed 30× whole-genome re-sequencing. Darmor-bzh as the reference genome was aligned to sequence data from the two bulks and parents. QTL-seq and PoPoolation2 workflow were applied to identify the candidate region of the white petal gene. A major candidate region was identified on chromosome C03 (52-54 Mb) of Darmor-bzh. The insertion-deletion (InDel) sites can be visualized in candidate interval by Integrative Genomics Viewer (IGV). Based on these Indel variations, we used Vector and Blast to design InDel primers. Eight InDel markers closely linked to the white petal gene were screened by Polyacrylamide gel electrophoresis (PAGE). In summary, these results provide a basis for fine mapping white petal gene and InDel molecular marker located on functional genes as well as molecular marker assisted selection breeding.

Key words: Brassica napus L., re-sequencing, white petal genes, InDel markers

图1

染色体上∆(SNP-index)分布 蓝点: ∆(SNP-index)位点; 红线: 利用滑动窗口数计算∆(SNP-index)变化趋势; 绿线: 显著性为95%的阈值; 橘线: 显著性为99%的阈值。"

图2

白花基因定位于C03 (Chr13)染色体上"

图3

IGV可视化候选区间InDel变异"

表1

InDel引物序列"

引物名称
Primer name		 Chr. C03 InDel 正向引物序列
Forward sequence (5°-3°)		 反向引物序列
Reverse sequence (5°-3°)
InDel-2 52375493-52375505 D10 GCTAAGCGTGTATCTTATCCT CGGGAATGGCGATCTAAAAA
InDel-3 52396484 I14 GAAAAACCATTGGAAATGCC TGGGAAGTTTGTTAAAGGGT
InDel-4 52348627-52348640 D12 GACAAGTCTTGGACAGCTAA CGAGAGGAATCCAAAGATGT
InDel-5 52377696-52377718 D20 TAGAGAGAGCTTAGACCTCC CGGCTATGAGTGATGTTCTT
InDel-6 52374279 I12 GGTTCTCTCTCTCTTCAGGTT TAATACTAATCGAAATTGTTGTCCC
InDel-8 52358540-52358551 D11 ATGCAACATCCAAAACACCT TTTTGCTCTTTTCGCGTTTG
InDel-9 52358775-52358788 D11 TCTGCGATCACGAATACCT CAAATCGAAAGGAAAGGAACA
InDel-10 52373701-52373721 D19 ACGTTAAAACAGCTTCACTTTC TCCGTGTCTGCCAAGTATC

图4

黄花和白花亲本及F2代单株电泳 1: 黄花亲本; 2: 白花亲本; 3~13: F2群体11个黄花单株; 14~24: F2群体11个白花单株。M: 20 bp梯度。"

[1] 王汉中. 以新需求为导向的油菜产业发展战略. 中国油料作物学报, 2018, 40:613-617.
Wang H Z. New-demand oriented oilseed rape industry developing strategy. Chin J Oil Crop Sci, 2018, 40:613-617 (in Chinese with English abstract).
[2] 阴长发, 官春云. 油菜花色研究进展. 作物研究, 2013, 27:403-408.
Yin C F, Guan C Y. A review of rapeseed flower color. Crop Res, 2013, 27:403-408 (in Chinese with English abstract).
[3] 刘后利. 油菜的遗传和育种. 上海: 上海科学技术出版社, 1985. pp 76-77.
Liu H L. Heredity and Breeding of Rape. Shanghai: Shanghai Scientific and Technical Publishers, 1985. pp 76-77(in Chinese)
[4] Chen B, Heneen W, Jonsson R. Brassica napus L Brassica napus L. Plant Breed, 1988, 100:147-149.
doi: 10.1111/pbr.1988.100.issue-2
[5] 戚存扣, 傅寿仲. 甘蓝型油菜白花性状的遗传. 中国油料作物学报, 1992, 1(3):60-62.
Qi C K, Fu S Z. Genetic studies of white petals in Brassica napus L. Chin J Oil Crop Sci, 1992, 1(3):60-62 (in Chinese with English abstract).
[6] Pearson O H. Brassica oleracea L Brassica oleracea L. Am Naturalist, 1929, 63:561-565.
doi: 10.1086/280291
[7] 张豹. 甘蓝型油菜导入系构建、重要农艺性状QTL分析和白花基因克隆. 华中农业大学博士学位论文, 湖北武汉, 2015.
Zhang B. Development of Chromosome Segment Substitution Lines for QTL Analysis of Important Agronomic Traits and Cloning the White-flowered Gene in Brassica napus L. PhD Dissertation of Huazhong Agricultural University, Wuhan, Hubei, China, 2015 (in Chinese with English abstract).
[8] 张洁夫, 浦惠明, 戚存扣, 傅寿仲. 甘蓝型油菜花色性状的遗传研究. 中国油料作物学报, 2000, 22(3):1-4.
Zhang J F, Pu H M, Qi C K, Fu S Z. Inheritance of flower color character in oilseed rape Brassica napus L. Chin J Oil Crop Sci, 2000, 22(3):1-4 (in Chinese with English abstract).
[9] 王翊, 景尚友, 吴刚, 任丽杰. 甘蓝型油菜白花性状在杂交油菜育种中的应用. 黑龙江农业科学, 2003, (6):13-14.
Wang Y, Jing S Y, Wu G, Ren L J. Application of the character of white flower of Brassica napus L. in hybrid breeding. Heilongjiang Agric Sci, 2003, (6):13-14 (in Chinese with English abstract).
[10] 文雁成, 张书芬, 王建平, 朱家成, 赵磊. 甘蓝型油菜白花性状的遗传学研究和白花胞质雄性不育系的选育. 中国农学通报, 2010, 26(1):95-97.
Wen Y C, Zhang S F, Wang J P, Zhu J C, Zhao L. Genetic studies of white Petals and selection of cytoplasmic male sterile line with white petals in Brassica napus L. Chin Agric Sci Bull, 2010, 26(1):95-97 (in Chinese with English abstract).
[11] 黄镇, 许婷, 班元元, 刘欢, 范胜栩, 杨丽, 徐爱遐. 甘蓝型油菜白花性状的遗传及AFLP标记. 华北农学报, 2012, 27(1):98-101.
Huang Z, Xu T, Ban Y Y, Liu H, Fan S X, Yang L, Xu A X. Genetic studies of white petals and AFLP markers linked to white petal gene in Brassica napus L. Acta Agric Boreali-Sin, 2012, 27(1):98-101 (in Chinese with English abstract).
[12] 邓昌蓉, 赵志刚, 余青兰. 人工合成甘蓝型油菜花色变异后代的遗传研究. 北方园艺, 2014, (18):14-17.
Deng C R, Zhao Z G, Yu Q L. The genetic studies of the flower color variation’s offsprings in artificial synthesis of Brassica napus. Northern Hortic, 2014, (18):14-17 (in Chinese with English abstract).
[13] 董育红, 田建华, 李殿荣, 郭蔼光, 孔建, 赵小萍. 甘蓝型油菜白花基因的RAPD标记. 西北农林科技大学学报, 2005, 33(10):57-61.
Dong Y H, Tian J H, Li D R, Guo A G, Kong J, Zhao X P. RAPD markers linked to white-petal gene in Brassica napus L. J Northwest A&F Univ, 2005, 33(10):57-61 (in Chinese with English abstract).
[14] 黄萌, 张建栋, 陈培峰, 宋英, 孙华. 甘蓝型油菜白花性状的遗传规律. 江苏农业科学, 2017, 45(20):83-84.
Huang M, Zhang J D, Chen P F, Song Y, Sun H. Genetic law of white flower traits in Brassica napus L. Jiangsu Agric Sci, 2017, 45(20):83-84 (in Chinese with English abstract).
[15] 田露申, 牛应泽, 余青青, 郭世星, 柳丽. 甘蓝型油菜白花性状的主基因+多基因遗传分析. 中国农业科学, 2009, 42:3987-3995.
Tian L S, Niu Y Z, Yu Q Q, Guo S X, Liu L. Genetic analysis of white flower color with mixed model of major gene plus polygene in Brassica napus L. Sci Agric Sin, 2009, 42:3987-3995 (in Chinese with English abstract).
[16] 刘雪平, 涂金星, 陈宝元, 傅廷栋. 人工合成甘蓝型油菜中花色与芥酸含量的遗传连锁分析. 遗传学报, 2004, 31:357-362.
Liu X P, Tu J X, Chen B Y, Fu T D. Identification of the linkage relationship between the flower colour and the content of erucic acid in the resynthesized Brassica napus L. Acta Genet Sin, 2004, 31:357-362 (in English with Chinese abstract).
[17] Han F Q, Yang C, Fang Z Y, Yang L M, Zhang M, Lyu H H, Liu Y M, Li Z S, Liu B, Yu H L, Liu X P, Zhang Y Y. cpc-1) in Brassica oleracea cpc-1) in Brassica oleracea. Mol Breed, 2015, 35:160.
doi: 10.1007/s11032-015-0354-x
[18] Huang Z, Ban Y Y, Bao R, Zhang X X, Xu A X, Ding J. Brassica napus L Brassica napus L. New Zealand J Crop Hortic Sci, 2014, 111:117.
[19] Zhang X X, Li R H, Niu S L, Chen L, Gao J, Wen J, Yi B, Ma C Z, Tu J X, Fu T D, Shen J X. Brassica juncea white-flowered mutant Bjpc2 using the whole-genome resequencing Brassica juncea white-flowered mutant Bjpc2 using the whole-genome resequencing. Mol Genet Genomics, 2017, 293:359-370.
doi: 10.1007/s00438-017-1390-5
[20] Xiao S, Xu J, Li Y, Zhang L, Shi S, Shi S, Wu J, Liu K. Brassica napus using a genome-walking technique Brassica napus using a genome-walking technique. Genome, 2007, 50:611-618.
doi: 10.1139/G07-044
[21] 陈雪, 王瑞, 井付钰, 张胜森, 贾乐东, 段谋正, 吴宇. 基于二代测序的甘蓝型油菜白花基因候选区间定位及连锁标记验证. 中国农业科学, 2020, 53:1108-1117.
Chen X, Wang R, Jing F Y, Zhang S S, Jia L D, Duan M Z, Wu Y. Location and linkage markers for candidate interval of the white petal gene in Brassica napus L. by next generation sequencing. Sci Agric Sin, 2020, 53:1108-1117 (in Chinese with English abstract).
[22] Zhang B, Liu C, Wang Y, Yao X, Wang F, Wu J, King G J, Liu K. Brassica species Brassica species. New Phytol, 2015, 206:1513-1526.
doi: 10.1111/nph.13335 pmid: 25690717
[23] Yao Y M, Li K X, Liu H D, Duncan R W, Guo S M, Xiao L, Du D Z. Bnpc 1) in spring Brassica napus L. to a 151-kb region Bnpc 1) in spring Brassica napus L. to a 151-kb region. Euphytica, 2017, 213:165.
doi: 10.1007/s10681-017-1959-4
[24] 丁戈, 陈伦林, 邹小云, 李书宇, 熊洁, 邹晓芬, 宋来强. 甘蓝型油菜桔黄花色基因的QTL-seq遗传分析及InDel分子标记开发. 分子植物育种, 2019, 17:3983-3992.
Ding G, Chen L L, Zou X Y, Li S Y, Xiong J, Zou X F, Song L Q. QTL-seq genetic analysis and InDel marker development of orange petel color gene in Brassica napus. Mol Plant Breed, 2019, 17:3983-3992 (in Chinese with English abstract).
[25] Mithra S V A, Kar M K, Mohapatra T, Robin S, Sarla N, Seshashayee M, Singh K, Singh N K, Sharma R P. DBT propelled national effort in creating mutant resource for functional genomics in rice. Curr Sci, 2016, 110:543-548.
doi: 10.18520/cs/v110/i4/543-548
[26] Wei F J, Droc G, Guiderdoni E, Hsing Y I C. International consortium of rice mutagenesis: Resources and beyond. Rice, 2013, 6:39.
doi: 10.1186/1939-8433-6-39
[27] Tsuda M, Kaga A, Anai T, Shimizu T, Sayamat, Takagi K, Machita K, Watanabe S, Nishimura M, Yamada N, Mori S, Sasaki H, Kanamori H, Katayose Y, Ishimoto M. Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing. BMC Genomics, 2015, 16:1014.
doi: 10.1186/s12864-015-2079-y pmid: 26610706
[28] Just D, Garcia V, Fernandez L, Bres C, Mauxion J P, Petit J, Jorly J, Assali J, Bournonville C, Ferrand C, Baldet P, Lemaire- Chamley M, Mori K, Okabe Y, Ariizumi T, Asamizu E, Ezura H, Rothan C. Micro-Tom mutants for functional analysis of target genes and discovery of new alleles in tomato. Plant Biotechnol J, 2013, 30:225-231.
[29] Lin T, Wang S H, Zhong Y, Gao D L, Cui Q Z, Chen H M, Zhang Z H, Shen H L, Weng Y Q, Huang S W. A truncated F-box protein confers the dwarfism in cucumber. J Genet Genomics, 2016, 43:223-226.
doi: 10.1016/j.jgg.2016.01.007
[30] Lun Y Y, Wang X, Zhang C Z, Yang L, Gao D L, Chen H M, Huang S W. A CsYcf54 variant conferring light green coloration in cucumber. Euphytica, 2016, 208:509-517.
doi: 10.1007/s10681-015-1592-z
[31] Zhou Q, Wang S H, Hu B W, Chen H M, Zhang Z H, Huang S W. An accumulation and replication of chloroplasts 5 gene mutation confers light green peel in cucumber. J Integr Plant Biol, 2015, 57:936-942.
doi: 10.1111/jipb.12355
[32] Takagi H, Abe A, Yoshid A K, Kosugi S, Natsume S, Mitsuoka C, Uemura A, Utsushi H, Tamiru M, Takumo S, Innan H, Cano L M, Kamoun S, Terauchi R. QTL-seq: rapid mapping of quantitative trait loci in rice by whole genome resequencing of DNA from two bulked populations. Plant J, 2013, 74:174-183.
doi: 10.1111/tpj.2013.74.issue-1
[33] Robert K, Ram Vinay P, Christian S. PoPoolation2: Identifying differentiation between populations using sequencing of pooled DNA samples (Pool-Seq). Bioinformatics, 2011, 27:3435-3436.
doi: 10.1093/bioinformatics/btr589 pmid: 22025480
[34] Hua Y P, Zhang D D, Zhou T, He M L, Ding G D, Shi L, Xu F S. Transcriptomics-assisted quantitative trait locus fine mapping for the rapid identification of a nodulin 26-like intrinsic protein gene regulating boron efficiency in allotetraploid rapeseed. Plant Cell Environ, 2016, 39:1601-1618.
doi: 10.1111/pce.v39.7
[35] 淡亚彬. 甘蓝型油菜桔红花色基因和心叶紫色基因的初步定位. 青海大学硕士学位论文, 青海西宁, 2016.
Dan Y B. Primary Mapping of the Orange Flower Gene and Central Leaf Color Gene in Brassica napus L. MS Thesis of Qinghai University, Xining, Qinghai, China, 2016 (in Chinese with English abstract)
[36] 潘存红, 王子斌, 马玉银, 殷跃军, 张亚芳, 左示敏, 陈宗祥, 潘学彪. InDel和SNP标记在水稻图位克隆中的应用. 中国水稻科学, 2007, 21:447-453.
Pan C H, Wang Z B, Ma Y Y, Yin Y J, Zhang Y F, Zuo S M, Chen Z X, Pan X B. InDel and SNP markers and their application in map-based cloning of rice genes. Chin J Rice Sci, 2007, 21:447-453 (in Chinese with English abstract).
[37] 胡坤. 玉米与大刍草InDel标记的开发及遗传连锁图谱的构建. 四川农业大学硕士学位论文, 四川雅安, 2014.
Hu K. The Development of Maize and Teosinte InDel Markers and Genetic Linkage Map Construction. MS Thesis of Sichuan Agricultural University, Ya’an, Sichuan, China, 2014 (in Chinese with English abstract).
[38] 吴迷, 汪念, 沈超, 黄聪, 温天旺, 林忠旭. 基于重测序的陆地棉InDel标记开发与评价. 作物学报, 2019, 45:196-203.
doi: 10.3724/SP.J.1006.2019.84100
Wu M, Wang N, Shen C, Huang C, Wen T W, Lin Z X. Development and evaluation of InDel markers in cotton based on whole-genome re-sequencing data. Acta Agron Sin, 2019, 45:196-203 (in Chinese with English abstract).
[39] 徐婷婷, 汪巧玲, 邹淑琼, 狄佳春, 杨欣, 朱银, 赵涵, 颜伟. 基于高通量测序的大麦InDel标记开发及应用. 作物学报, 2020, 46:1340-1355.
doi: 10.3724/SP.J.1006.2020.91076
Xu T T, Wang Q L, Zou S Q, Di J C, Yang X, Zhu Y, Zhao H, Yan W. Development and application of InDel markers based on high throughput sequencing in barley. Acta Agron Sin, 2020, 46:1340-1355 (in Chinese with English abstract).
[40] 周新桐, 郭青青, 陈雪, 李加纳, 王瑞. GBS高密度遗传连锁图谱定位甘蓝型油菜粉色花性状. 作物学报, 2021, 47:587-598.
doi: 10.3724/SP.J.1006.2021.04115
Zhou X T, Guo Q Q, Chen X, Li J N, Wang R. Construction of a high-density genetic map using genotyping by sequencing (GBS) for quantitative trait loci (QTL) analysis of pink petal trait in Brassica napus L. Acta Agron Sin, 2021, 47:587-598 (in Chinese with English abstract).
[1] 马毅娜, 吴晓明玉, 李藕琪, 王圆, 陈丽, 张盈川, 赵伦, 文静, 傅廷栋, 沈金雄. Bna-miR1040-EIF3A模块调控油菜开花时间的功能研究[J]. 作物学报, 2026, 52(2): 349-362.
[2] 王彬, 蒙姜宇, 邱浩良, 贺亚军, 钱伟. 甘蓝型油菜BnaDUF579基因家族的鉴定与表达模式分析[J]. 作物学报, 2025, 51(8): 2100-2110.
[3] 夏琦, 郭滢, 王坤美, 王思忆, 巨建业, 彭雅雯, 刘忠松, 夏石头. 甘蓝型油菜种子和种皮中水杨酸含量与原花色素积累的关系研究[J]. 作物学报, 2025, 51(5): 1189-1197.
[4] 王晓琳, 刘忠松, 康雷, 杨柳. 甘蓝型油菜角果长度和每角粒数基因定位以及角果皮转录组动态分析[J]. 作物学报, 2025, 51(4): 888-899.
[5] 张琴, 戴成, 马朝芝. 生长素响应报告基因转化甘蓝型油菜及各组织GUS动态信号分析[J]. 作物学报, 2025, 51(3): 667-675.
[6] 孙程明, 周晓婴, 陈锋, 张维, 王晓东, 彭琦, 郭月, 高建芹, 胡茂龙, 付三雄, 张洁夫. 长链非编码RNA (lncRNA)在甘蓝型油菜分枝角度调控中的功能分析与预测[J]. 作物学报, 2025, 51(3): 559-567.
[7] 黄绒, 周渠晨, 陈楚铭, 罗倩, 易东, 杜常欢, 黄祥宇, 盛锋, 杜雪竹. 过表达BnNRT2.3-like对油菜氮素吸收利用及产量的影响[J]. 作物学报, 2025, 51(12): 3184-3197.
[8] 巨建业, 杨柳, 陈浩, 康雷, 夏石头, 刘忠松. 单细胞核转录组分析揭示油菜种皮分化过程和种子颜色差异原因[J]. 作物学报, 2025, 51(11): 2860-2874.
[9] 张雯, 李玉, 王创, 石磊, 丁广大. 甘蓝型油菜磷转运蛋白BnaPT48的功能研究[J]. 作物学报, 2025, 51(11): 2983-2995.
[10] 胡志康, 舒雨, 王会, 杨莹莹, 廖俊宇, 刘佳, 成洪涛, 郭晨, 张园园, 刘胜毅, 胡琼, 梅德圣, 李超. 甘蓝型油菜苗期耐碱性种质综合鉴定与评价[J]. 作物学报, 2025, 51(10): 2681-2692.
[11] 王晨, 贺丹, 姚敏, 邱萍, 何昕, 熊兴华, 康雷, 刘忠松, 钱论文. 基于转录组分析鉴定甘蓝型油菜开花候选基因以及BnaCOR27功能验证[J]. 作物学报, 2025, 51(10): 2693-2704.
[12] 徐林珊, 郜耿东, 王宇, 王家星, 杨吉招, 武亚瑞, 张宵寒, 常影, 李真, 谢雄泽, 龚德平, 王晶, 葛贤宏. 甘蓝型油菜漆酶基因家族成员表达模式及与茎秆抗折力的关联分析[J]. 作物学报, 2025, 51(1): 134-148.
[13] 匡博文, 韦妳, 刘金典, 陈美燕, 毛兴洁, 段维兴, 杨细平. 基于甘蔗及其近缘属参考基因组开发SSR标记及数据库[J]. 作物学报, 2025, 51(1): 103-116.
[14] 李嘉欣, 黄莹, 吴潞梅, 赵伦, 易斌, 马朝芝, 涂金星, 沈金雄, 傅廷栋, 文静. 甘蓝型油菜BnaSLY1基因进化分析及功能研究[J]. 作物学报, 2025, 51(1): 44-57.
[15] 钟元, 朱天宇, 戴成, 马朝芝. 耐亚磷酸盐除草剂转基因油菜的创建和抗性评价[J]. 作物学报, 2024, 50(5): 1158-1171.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2