欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (7): 1274-1281.doi: 10.3724/SP.J.1006.2009.01274

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦抗条锈病基因YrZH84的RGAP标记及其应用

殷贵鸿1,2,3,王建武1,2,闻伟锷2,何中虎2,4,李在峰5,王辉1,夏先春2,*   

  1. 1西北农林科技大学农学院，陕西杨凌712100；2中国农业科学院作物科学研究所/国家小麦改良中心/高家农作物基因资源与基因改良重大科学工程，北京100081；3周口市农业科学院，河南周口466001；4CIMMYT中国办事处，北京100081；5河北农业大学植物保护学院，河北保定071001
  • 收稿日期:2008-12-26 修回日期:2009-03-23 出版日期:2009-07-12 网络出版日期:2009-05-19
  • 通讯作者: 夏先春, Tel: 010-68918610, E-mail: xiaxianchun@caas.net.cn
  • 基金资助:

    本研究由国家高技术研究发展计划（863计划）项目（2006AA10Z1A7和2006AA100102）资助。

Mapping of Wheat Stripe Rust Resistance Gene YrZH84 with RGAP Markers and Its Application

YIN Gui-Hong1,2,3,WANG Jian-Wu1,2,WEN Wei-E2,HE Zhong-Hu2,4,LI Zai-Feng5,WANG Hui1,XIA Xian-Chun2*   

  1. 1College of Agronomy,Northwest A&F University,Yangling 712100,China;2Institute of Crop Sciences/National Wheat Improvement Center/National Key Facility for Crop Gene Resources and Genetic Improvement,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3Zhoukou Academy of Agricultural Sciences,Zhoukou 466001,China;4CIMMYT China Office,Beijing 100081;5College of Plant Protection,Agricultural University of Hebei,Baoding 071001,China
  • Received:2008-12-26 Revised:2009-03-23 Published:2009-07-12 Published online:2009-05-19
  • Contact: XIA Xian-Chun, Tel: 010-68918610, E-mail: xiaxianchun@caas.net.cn

PDF

1830

被引次数

30

摘要：

利用RGAP标记及基于标记基因型和表型选择的分离群体分组分析法，对小麦抗条锈病骨干亲本周8425B和感病品种中国春及其F2分离群体进行分析，获得了与抗条锈基因YrZH84紧密连锁的RGAP标记Xrga-1，连锁距离为0.8 cM。其RGA片段长度为343 bp，BLAST分析结果表明，该RGA序列与已克隆的大麦抗秆锈病基因Rpg1核苷酸序列同源性为93%，与大麦抗白粉病基因Mla家族的核苷酸序列同源性为92%。用标记Xrga-1对黄淮麦区58个小麦品种(系)进行了检测，结合系谱分析和抗病性鉴定结果，发现周8425B的衍生品种周麦11、周麦17、周麦20、周麦22、矮抗58、04中36、源育3号、05中37、宛抗18、豫展10号携带抗条锈病基因YrZH84。这些研究结果对小麦抗条锈病分子育种和YrZH84基因克隆有重要作用。

关键词: 普通小麦, 抗条锈病基因, RGAP标记

Abstract:

Zhou 8425B, with an effective stripe rust resistance gene YrZH84, is a hall-mark parent line of wheat (Triticum aestivum L.) in the Yellow-Huai rivers wheat growing region. An F2 population derived from the cross Zhou 8425B/Chinese Spring and their parents were used forpolymorphism analysis employing the resistance gene-analog polymorphism (RGAP) markers and bulked segregant analysis (BSA) based on marker genotypes and phenotypes selection. An RGAP marker Xrga-1 was identified, which was tightly linked to the stripe rust resistance gene YrZH84, with a genetic distance of 0.8 cM. The fragment amplified by Xrga-1 was 343 bp. BLAST analysis indicated that the rga-1 showed 93% of identical nucleotide sequences to barley stem rust resistance gene Rpg1, and 92% to barley powdery mildew resistance gene Mla homology family. Fifty-eight cultivars from Yellow-Huai rivers wheat growing region were genotyped by the RGAP marker Xrga-1. From the information of genotypes, pedigrees and resistance responses, the cultivars Zhoumai 11, Zhoumai 20, Zhoumai 22, Aikang 58, 04 Zhong 36, Yuanyu 3, 05 Zhong 37, Wuankang 18, and Yuzhan 10 were identified to have the resistance gene YrZH84. These cultivars were the derivatives of Zhou 8425B. These results will greatly benefit the marker-assisted selection in wheat and cloning of the resistance gene YrZH84.

Key words: Common wheat, Stripe rust resistance gene, RGAP marker

[1]Line R F. Stripe rust of wheat and barley in North America: A retrospective historical review. Annu Rev Phytopathol, 2002, 40: 75-118

[2]Li Z-Q(李振岐), Zeng S-M(曾士迈).Wheat Rust in China (中国小麦锈病). Beijing: China Agriculture Press, 2002. pp 1-3 (in Chinese)

[3]Wan A M, Zhao Z H, Chen X M, He Z H, Jin S L, Jia Q Z, Yao G, Yang J X, Wang B T, Li G B, Bi Y Q, Yuan Z Y. Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002.Plant Dis, 2004, 88: 896-904
[4].Zhao Z-H(赵中华). Characteristic of national stripe rust epidemiology in 2003 and its management strategy. China Plant Prot (中国植保导刊), 2004, 24(2): 16-18 (in Chinese)

[5]The National Agro-Tech Extension and Service Center (全国农业技术推广服务中心). Prediction of the outbreak of significant disease and pest for crops in national scale in 2004. China Plant Prot (中国植保导刊), 2004, 24(3): 21-22 (in Chinese)

[6]Zhang Y-J(张跃进), Wang J-Q(王建强), Jang Y-Y(姜玉英), Feng X-D(冯晓东), Xia B(夏冰). Prediction of the outbreak of disease and pest for crops in national scale in 2005. China Plant Prot(中国植保导刊), 2005, 25(4): 28-30 (in Chinese)

[7]Zhang Y-J(张跃进), Wang J-Q(王建强), Jiang Y-Y(姜玉英), Feng X-D(冯晓东), Xia B(夏冰). Prediction of the outbreak of disease and pest for crops in national scale in 2006. China Plant Prot(中国植保导刊), 2006, 26(4): 5-8 (in Chinese)

[8]Zhang Y-J(张跃进), Wang J-Q(王建强), Jiang Y-Y(姜玉英), Feng X-D(冯晓东), Xia B(夏冰), Liu Y(刘宇), Zeng J(曾娟). Prediction of the outbreak of disease and pest for crops in national scale in 2007. China Plant Prot(中国植保导刊), 2007, 27(2): 32-35 (in Chinese)

[9]McIntosh R A, Devos K M, Dubcovsky J, Rogers W J, Morris C F, Appels R, Somers D J, Anderson O A. Catalogue of Gene Symbols for Wheat, 2008 (supplement).
[2008-12-21], http://www.wheat.pw.usda.gov/GG2/Triticum/Wgc/2008/Catalogue 2008. pdf

[10]Yan G P, Chen X M, Line R F, Wellings C R. Resistance gene-analog polymorph- hism markers co-segregating with the Yr5 gene for resistance to wheat stripe rust. Theor Appl Genet, 2003, 106: 636-643

[11]Shi Z X, Chen X M, Line R F, Leung H, Wellings C R. Development of resistance gene-analog polymorphism markers for the Yr9 gene resistance to wheat stripe rust. Genome, 2001, 44: 509-516

[12]Wen W E, Li G Q, He Z H, Yang W Y, Xu M L, Xia X C. Development of an STS marker tightly linked to Yr26 against wheat stripe rust using the resistance gene-analog polymorphism (RGAP) technique. Mol Breed, 2008, 22: 507-515
Lin F, Chen X M. Genetics and molecular mapping of genes race-specific all-stageresistance and non-race-specific high-temperature adult-plant resistance to stripe rust in spring wheat cultivar Alpowa. Theor Appl Genet, 2007, 114: 1277-1287

[14]Xiao W K, Zhao J, Fan S C, Li L, Dai J R, Xu M L. Mapping of genome-wide resistance gene analogs (RGAs) in maize (Zea mays L.). Theor Appl Genet, 2007, 115: 501-508

[15]Van Ooijen G, Van den Burg H A, Cornelissen B J, Takken F L. Structure and function of resistance proteins in solanaceous plants. Annu Rev Phytopathol,2007, 45: 43-72

[16]Chen R G, Zhang L Y, Zhang J H, Xiao J H, Li H X, Ye Z B. CaMi, a rootknot nematode resistance gene from hot pepper (Capsium annuum L.) confersne- matode resistance in tomato. Plant Cell Rep, 2007, 26: 895-905

[17]Chen X M, Line R F, Leung H. Genome scanning for resistance-gene analogs in rice, barley, and wheat by high-resolution electrophoresis. Theor Appl Genet, 1998, 97: 345-355

[18]Lin F, Chen X M. Molecular mapping of genes for race-specific overall resistance to stripe rust in wheat cultivar express. Theor Appl Genet, 2008, 116: 797-806

[19]Madsen L H, Collins N S, Rakwalska M, Backes G, SandalN, Krusell L, Jensen J, Waterman E H. Barley disease resistance gene analogs of the NBS-LRR class: identification and mapping. Mol Genet Gen, 2003, 269: 150-161

[20]Yan G P, Chen X M. Molecular mapping of a recessive gene for resistance to stripe rust in barley. Theor Appl Genet, 2006, 113: 529-537

[21]Yan, G P, Chen X M. Molecular mapping of the rps:Arecessive gene for resistance to stripe rust in BBA 2890 barley. Phytopathology, 2007, 97: 668-673


[22]Graham M A, Marek L F, Lohnes D,

Cregan P, Shoemaker R C. Expression and genome organization of resistance gene analogs in soy bean. Genome, 2000, 43: 86-93

[23]Quint M, Mihaljevic R, Dussle C M, Xu M L, Melchinger A E, Lübberstedt T. Development of RGA-CAPS markers and genetic mapping of candidate genes for sugarcane mosaic virus resistance in maize. Theor Appl Genet, 2002, 105: 355-363


[24]Ramalingam J, Vera Cruz C M, Kukreja K, Chittoor J M,

Wu J L, Lee S W, Baraoidan M, George M L, Cohen M B, Hulbert S H, Leach J E, Leung H. Candidate defense genes from rice, barley, and maize and their association with qualitative and quantitative resistance in rice. Mol Plant Microbe Interact, 2003, 16: 14-24

[25]Li Z F, Zheng T C, He Z H, Li G Q,Xu S C, Li X P, Yang G Y, Singh R P, Xia X C. Molecular tagging of stripe rust resistance gene YrZH84 in Chinese wheat line Zhou 8425B. Theor Appl Genet, 2006, 112: 1098-1103

[26]Lincoln S, Daly M, Lander E. Constructing genetic maps with MAPMAKER /EXP3.0, 3rd edn. In: Whitehead Institute Technical Report, Cambridge, Massachusetts, 1992

[27]Ling H Q, Zhu Y, Keller B. High-resolution mapping of the leaf rust disease resistance gene Lr1 in wheat and characterization of BAC clones from the Lr1 locus. Theor Appl Genet, 2003, 106: 875-882
[28]Smith P H, Hadfield J, Hart N J, Koebner R M, Boyd L A. STS markers for the wheat yellow rust resistance gene Yr5 suggest a NBS-LRR-type resistance gene cluster. Genome, 2007,50: 259-265
[1] 靳义荣, 刘金栋, 刘彩云, 贾德新, 刘鹏, 王雅美. 普通小麦氮素利用效率相关性状全基因组关联分析[J]. 作物学报, 2021, 47(3): 394-404.
[2] 张平平,姚金保,王化敦,宋桂成,姜朋,张鹏,马鸿翔. 江苏省优质软麦品种品质特性与饼干加工品质的关系[J]. 作物学报, 2020, 46(4): 491-502.
[3] 杨芳萍,刘金栋,郭莹,贾奥琳,闻伟鄂,巢凯翔,伍玲,岳维云,董亚超,夏先春. 普通小麦‘Holdfast’条锈病成株抗性QTL定位[J]. 作物学报, 2019, 45(12): 1832-1840.
[4] 王林生,张雅莉,南广慧. 普通小麦-大赖草易位系T5AS-7LrL·7LrS分子细胞遗传学鉴定[J]. 作物学报, 2018, 44(10): 1442-1447.
[5] 赵德辉, 张勇, 王德森, 黄玲, 陈新民, 肖永贵, 阎俊, 张艳, 何中虎. 北方冬麦区新育成优质品种的面包和馒头品质性状[J]. 作物学报, 2018, 44(05): 697-705.
[6] 苗永杰, 阎俊, 赵德辉, 田宇兵, 闫俊良, 夏先春, 张勇, 何中虎. 黄淮麦区小麦主栽品种粒重与籽粒灌浆特性的关系[J]. 作物学报, 2018, 44(02): 260-267.
[7] 肖永贵,Susanne DREISIGACKER,Claudia NU?EZ-RíOS,胡卫国,夏先春,何中虎. 基于FLUOstar平台的小麦dsDNA荧光定量与基因型分析[J]. 作物学报, 2017, 43(07): 947-953.
[8] 董雪,刘梦,赵献林,冯玉梅,杨燕. 普通小麦近缘种低分子量麦谷蛋白亚基Glu-A3基因的分离和鉴定[J]. 作物学报, 2017, 43(06): 829-838.
[9] 刘凯,邓志英,张莹,王芳芳,刘佟佟,李青芳,邵文,赵宾,田纪春*,陈建省*. 小麦茎秆断裂强度相关性状QTL的连锁和关联分析[J]. 作物学报, 2017, 43(04): 483-495.
[10] 宫希,蒋云峰,徐彬杰,乔媛媛,华诗雨,吴旺,马建,周小鸿,祁鹏飞,兰秀锦. 利用普通六倍体小麦和西藏半野生小麦杂交衍生的重组自交系定位小麦芒长QTL[J]. 作物学报, 2017, 43(04): 496-500.
[11] 王鑫,马莹雪,杨阳,王丹峰,殷慧娟,王洪刚. 小麦矮秆种质SN224的鉴定及农艺性状QTL分析[J]. 作物学报, 2016, 42(08): 1134-1142.
[12] 孔欣欣,张艳,赵德辉,夏先春,王春平,何中虎. 北方冬麦区新育成优质小麦品种面条品质相关性状分析[J]. 作物学报, 2016, 42(08): 1143-1159.
[13] 刘凯,邓志英,李青芳,张莹,孙彩铃,田纪春*,陈建省*. 利用高密度SNP 遗传图谱定位小麦穗部性状基因[J]. 作物学报, 2016, 42(06): 820-831.
[14] 李文爽，夏先春，何中虎. 普通小麦类胡萝卜素组分的超高效液相色谱分离方法[J]. 作物学报, 2016, 42(05): 706-713.
[15] 岳爱琴，李昂，毛新国，昌小平，柳玉平，李润植，景蕊莲. 小麦果聚糖合成酶基因6-SFT-D多态性及其与6-SFT-A2的累加效应[J]. 作物学报, 2016, 42(01): 11-18.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2