β-葡聚糖,抗氧化作用," /> β-葡聚糖,抗氧化作用,"/> 裸燕麦核心种质的抗氧化特性
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作物学报 ›› 2010, Vol. 36 ›› Issue (06): 988-994.doi: 10.3724/SP.J.1006.2010.00988

• 耕作栽培·生理生化 • 上一篇    下一篇

裸燕麦核心种质的抗氧化特性

任祎1,2,平华3,任贵兴2,*   

  1. 1山西农业大学农学院，山西太谷030801；2中国农业科学院作物科学研究所，北京100081；3北京农产品质量检测与农田环境监测技术研究中心，北京100097
  • 收稿日期:2009-11-30 修回日期:2010-02-08 出版日期:2010-06-12 网络出版日期:2010-04-20
  • 通讯作者: 任贵兴, Email: renguixing@caas.net.cn, Tel: 010-62115596
  • 基金资助:

    本研究由国家公益性研究专项基金（2005DIA4J019）和山西农业大学科研启动基金（XB2009001）资助。

Antioxidant Property of Naked Oat Core Collections

REN Yi1,2,PING Hua3,REN Gui-Xing2,*   

  • Received:2009-11-30 Revised:2010-02-08 Published:2010-06-12 Published online:2010-04-20
  • Contact: REN Gui-Xing,Email:renguixing@caas.net.cn, Tel: 010-62115596

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1522

被引次数

29

摘要：

为了解裸燕麦种质资源的抗氧化特性及其抗氧化作用的物质基础，选用和检测了120份国内外裸燕麦种质。其DPPH清除能力及总酚、生物碱和β-葡聚糖含量的变幅分别为4.34~179.98 mg L-1、250.99~807.88 mg kg-1、25.21~347.55 mg kg-1和0.59%~9.69%，表现出明显的基因型差异，不同来源地和不同种质类型裸燕麦的抗氧化能力和生物碱含量差异较大。采用K-mean cluster快速聚类法将其聚为6大类群，分别由20、39、2、22、12和25份种质组成。燕麦籽粒抗氧化能力与其总酚、生物碱含量呈极显著(P＜0.01)正相关，但与β-葡聚糖含量无显著相关性。表明裸燕麦抗氧化能力与其籽粒中所含的总酚和生物碱类物质密切相关。本研究还筛选出一批高抗氧化的燕麦种质资源，有望用于高抗氧化性燕麦品种的培育。

关键词: 裸燕麦, 生物碱, 总酚, β-葡聚糖')">β-葡聚糖, 抗氧化作用

Abstract:

Naked Oat (Avena nuda L.) originated in China, has a high values in nutrition and medicine. Besides dietary fiber and nutrients, various natural antioxidants, such as phenolic compounds, phenolic acids, avenanthramides (unique to oats) and phytosterols are also contained in naked oat. All these compounds possess potential health-promoting properties, partly attributing to their antioxidant activity. Up to now, China has built the core of naked oat germplasm with the greatest diversity of naked oat types. The objective of this study was to determine the total phenol (TP), total avenanthramides (TAV), β-glucan contents and the antioxidant activity of oat cultivars from main productive areas of China as well as Former Soviet Union and North America. The correlation of antioxidant activities with contents of these components was also statistically investigated, which is benefit for oat breeding. The antioxidant activities, contents of TP, TAV, and β-glucan of 120 naked oat accessions were evaluated by Folin-Ciocalteu method, HPLC analysis and enzymatic method, respectively. Meanwhile, the antioxidant capacity was determined by 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) scavenging ability method. Significantly genotypic variations were observed, and contents of TP, TAV, and β-glucan were 4.34–179.98 mg L-1, 250.99–807.88 mg kg-1, 25.21–347.55 mg kg-1, and 0.59–9.69% respectively. The 120 accessions were clustered into six groups using K-mean cluster method, which consisted of 20, 39, 2, 22, 12, and 25 accessions respectively. There were significantly positive correlations between antioxidant activity and the contents of TP and TAV. However, the correlation between β-glucan and DPPH was not significant. These results indicated that avenanthramides contributed greatly to the antioxidant property in naked oat grains. A relative wide variations in TAV and TP contents and compositions were mainly due to differences among cultivars. Duji from Sichuan province and Yumai 38 from Qinghai province with high contents of TP and TAV are expected to be used in further quality breeding of naked oat.

Key words: oat, Avenanthramides, Total phenolic, Beta-glucan, Antioxidant activity


[1]Zhao X-F(赵秀芳), Rong Y-P(戎郁萍), Zhao L-X(赵来喜).The collection and evaluation of oat (Avena sative) in China. Pratacult Sci (草业科学), 2007, 24(3): 36–39 (in Chinese with English abstract)


[2] Yang H-P(杨海鹏). History of naked oats. Inner Mongolia Agric Sci & Tech (内蒙古农业科技), 1979, (5): 33 (in Chinese)


[3] Wang M-Y(王茅雁), Qi X-L(齐秀丽), Zhang F-Y(张凤英). Progress in the research of oat molecular biology abroad. J Inner Mongolia Agric Univ (内蒙古农业大学学报), 2001, 22(4): 139–144 (in Chinese with English abstract)


[4] Zheng D-S(郑殿升), Lü Y-C(吕耀昌), Tian C-Y(田长叶), Zhao W(赵伟). Analysis on beta-glucan content of naked oat. J Plant Genet Resour (植物遗传资源学报), 2006, 7(1): 54–58 (in Chinese with English abstract)


[5] Deng W-H(邓万和), Wang Q(王强), Lü Y-C(吕耀昌), Zhou S-M(周素梅). Effects of cultivar and environment on β-glucan content in oats. J Chin Cereals & Oils Assoc (中国粮油学报), 2005, 20(2): 30–32 (in Chinese with English abstract)


[6] Dong J-L(董吉林), Shen R-L(申瑞玲). Compositional analysis of naked oat bran and nutrient β-glucan extraction. J Shanxi Agric Univ (山西农业大学学报), 2005, 25(1): 70–73 (in Chinese with English abstract)


[7] Wei H(魏红), Zhang Z-S(张泽生), Zhang M(张民), Gao W-W(高薇薇). Study on anti-oxidative activity: The extracts from naked oat bran. Food Res & Devel (食品研究与开发), 2007, 28(2): 14–17 (in Chinese with English abstract)


[8] Shi D-F(时东方), Ren C-Z(任长忠), Li L(李丽), Wang Q(王强), Zheng M-Z(郑梅竹), Liu C-M(刘春明). Analysis of components and study on antioxidant activity of phenolics in oats. J Anhui Agric Sci (安徽农业科学), 2008, 36(30): 13000–13002 (in Chinese with English abstract)


[9] Peters F N, Musher S. Oat flour as an antioxidant. Ind Eng Chem, 1937, 29: 146–151


[10] Peterson D M. Oat antioxidants. J Cereal Sci, 2001, 33: 115–129


[11] Dimberg L H, Theander O, Lingnert H. Avenanthramides-A group of phenolic antioxidants in oats. Cereal Chem, 1993, 70: 637–641


[12] Peterson D M, Hahn M J, Emmons C L. Oat avenanthramides exhibit antioxidant activities in vitro. Food Chem, 2002, 79: 473–478


[13] Ji L L, Lay D, Chung E, Fu Y, Peterson D M. Effects of avenanthramides on oxidant generation and antioxidant enzyme activity in exercised rats. Nutr Res, 2003, 23: 1579–1590


[14] Liu L P, Zubik L, Collins F W, Marko M, Meydani M. The antiatherogenic potential of oat phenolic compounds. Atherosclerosis, 2004, 175: 39–49


[15] Sur R, Nigam A, Grote D, Liebel F, Southall M D. Avenanthramides, polyphenols from oats, exhibit anti-inflammatory and anti-itch activity. Arch Dematol Res, 2008, 300: 569–574


[16] Peng C-L(彭长连), Chen S-W(陈少薇), Lin Z-F(林植芳), Lin G-Z(林桂珠). Detection of antioxidative capacity in plants by scavenging organic free radical DPPH. Prog Biochem Biophys (生物化学与生物物理进展), 2000, 27(6): 658–661 (in Chinese with English abstract)


[17] Brand W W, Cuvelier M E, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensm-Wiss Technol, 1995, 28: 25–30


[18] Yu L, Haley S, Perret J, Harris M, Wilson J, Qian M. Free radical scavenging properties of wheat extracts. J Agric Food Chem, 2002, 50: 1619–1624


[19] Ren Y(任祎), Ren G-X(任贵兴),Ma T-J(马挺军), Ping H(平华), Niu X-W(牛西午). Extraction and antioxidant activity of avenanthramides from oat bran. Trans CSAE (农业工程学报), 2008, 24(5): 265–269 (in Chinese with English abstract)


[20] Wang W-H(王稳航), Li Y(李玉), Li K(李琨). The phenoics composition and antioxdative properties in stored apple. Food Res & Devel (食品研究与开发), 2006, 27(1): 5–8 (in Chinese with English abstract)


[21] Yan S-L(严守雷), Wang Q-Z(王清章), Peng G-H(彭光华). Folin method to determine the total phenols content in the joints of lotus roots. J Huazhong Agric Univ (华中农业大学学报), 2003, 22(4): 412–414 (in Chinese with English abstract)


[22] Pan Y M, Wang K, Hang S Q, Wang H S, Mu X M. Antioxidant activity of microwave-assited extract of longan (Dimocarpus Longan Lour) peel. Food Chem, 2008, 106: 1264–1270


[23] Peterson D M, Wesenberg D M, Burrup D E. β-glucan content and its relationship to agronomic characteristics in elite oat germplasm. Crop Sci , 1995, 35: 965–970


[24] Xu J-R(徐金瑞), Zhang M-W(张名位), Liu X-H(刘兴华). Correlation between antioxidation, and content of total phenolics and anthocyanin in black soybean accessions. Sci Agric Sin (中国农业科学), 2006, 39(8): 1545–1552 (in Chinese with English abstract)


[25] Peterson D M, Wesenberg D M, Burrup D E, Erickson C A. Relationships among agronomic traits and grain composition in oat genotypes grown in different environments. Crop Sci, 2005, 45: 1249–1255


[26] Emmons C L, Peterson D M. Antioxidant activity and phenolic content of oat as affected by cultivar and location. Crop Sci, 2001,41: 1676–1681


[27] Bratt K, Sunnerheim K, Bryngelsson S, Fagerlund A, Engman L, Andersson R E, Dimberg L H. Avenanthramides in oats (Avena sativa L.) and structure –antioxidant activity relationships. J Agric Food Chem, 2003, 51: 594–600


[28] Kevin C M, Fred S, Marlyn A S, Patricia E V, Laura C Q, Pamela G H, Arlene T, Michael H D. Food products containing free tall oil-based phytosterols and oat β-glucan lower serum total and LDL cholesterol in hypercholesterolemic adults. J Nutr, 2003, 133: 808–813
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