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作物学报 ›› 2011, Vol. 37 ›› Issue (09): 1577-1584.doi: 10.3724/SP.J.1006.2011.01577

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

烟草六个重要性状的QTL定位

李华丽1，陈美霞1,**，周东新2，陈顺辉3，陶爱芬1，李延坤1，马红勃1，祁建民1,*，郭玉春1,*   

  1. 1福建农林大学 / 作物遗传育种与综合利用省部共建教育部重点实验室，福建福州350002；2 福建省龙岩市烟草科学研究所，福建龙岩364000；3福建省烟草科学研究所，福建福州350003
  • 收稿日期:2010-12-21 修回日期:2011-04-27 出版日期:2011-09-12 网络出版日期:2011-06-28
  • 通讯作者: 祁建民, E-mail: Qijm863@163.com; 郭玉春, E-mail: ycguo168@sina.com
  • 基金资助:

    本研究由国家烟草专卖局科技项目(110200601006)和国家烟草专卖局资助项目(国烟科[2005])资助。

QTL Analysis of Six Important Traits in Tobacco (Nicotiana tabacum L.)

LI Hua-Li1,CHEN Mei-Xia1,ZHOU Dong-Xin2,CHEN Shun-Hui3,TAO Ai-Fen1,LI Yan-Kun1,MA Hong-Bo1,QI Jian-Min1,*,GUO Yu-Chun1,*   

  1. 1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops / Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2 Fujian Longyan Tobacco Science Institute, Longyan 364000, China; 3 Fujian Tobacco Science Institute, Fuzhou 350003, China
  • Received:2010-12-21 Revised:2011-04-27 Published:2011-09-12 Published online:2011-06-28
  • Contact: 祁建民, E-mail: Qijm863@163.com; 郭玉春, E-mail: ycguo168@sina.com

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被引次数

25

摘要： 以烤烟台烟7号与白肋烟白肋21作为杂交亲本，后代自交衍生的127个F2和F2:3家系为材料，构建了全长3 483 cM包括26个连锁群、190个标记位点的烟草遗传连锁图谱。通过一年两地各3次重复的随机区组田间试验，测定烟叶烟碱、总氯、总钾、叶长、茎叶夹角、白粉病6个重要性状，采用混合线性模型的复合区间作图法定位QTL并分析其遗传互作效应。结果检测到2个烟碱相关QTL、2个总氯相关QTL、1个总钾相关QTL、4个叶长相关QTL、茎叶夹角和白粉病相关QTL各1个，其中6个加性效应QTL和4对加加上位性效应QTL。这11个基因位点遗传效应中除加性效应外，上位性效应也具有重要作用。

关键词: 烟草, 性状, QTL定位, 上位性

Abstract: The agronomic traits, chemical components and diseases are very important factors affecting the yield and leaf quality of tobacco. QTLs linked to the three aspects of traits can be used in marker-assisted selection for the yield and leaf quality improvement in tobacco. The objective of this study was to identify QTLs controlling important traits in tobacco, which could promote the basic research of molecular marker-assisted selection breeding. A population with 127 F2 and F2:3 individuals from the cross between Taiyan 7 and Baile 21 was used to establish a genetic linkage map of tobacco, 190 marker loci were mapped into 26 linkage groups which spanned a total map length of 3 483 cM. With a randomized complete block design, two location field tests with three replicates one year were conducted to charactererize six important traits, including nicotine, total chlorine, total kalium, leaf length, leaf angle, and powdery mildew. Utilizing data of field trials and MCIM method, QTLs for six important characters were mapped and their genetic interactions were analyzed. As the results, two QTLs for nicotine, two QTLs for total chlorine, one QTL for total kalium, four QTLs for leaf length, one QTL for leaf angle, and one QTL for powdery mildew were detected, of which six QTLs had additive effect, and the others were epistatic. The results indicated that epistatic QTLs, the additive QTLs also played important roles in the genetic basis of the six important traits with eleven gene loci in tobacco.

Key words: Tobacco (Nicotiana tobacum L.), Trait, QTL, Epistasis

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