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作物学报 ›› 2012, Vol. 38 ›› Issue (03): 423-428.doi: 10.3724/SP.J.1006.2012.00423

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻卷叶基因RL13的遗传分析和分子定位

田晓庆,桑贤春,赵芳明,李云峰,凌英华,杨正林,何光华*   

  1. 西南大学水稻研究所 / 转基因植物与安全控制重庆市重点实验室 / 南方山地农业教育部工程研究中心，重庆 400715
  • 收稿日期:2011-07-13 修回日期:2011-10-15 出版日期:2012-03-12 网络出版日期:2012-01-04
  • 通讯作者: 何光华, E-mail: hegh@swu.edu.cn
  • 基金资助:

    本研究由重庆市攻关项目(CSTC,2010AA1019)和国家转基因生物新品种培育重大专项(2009ZX08009-109B)资助。

Genetic Analysis and Molecular Mapping of a Rolled Leaf Gene RL13 in Rice (Oryza sativa L.)

TIAN Xiao-Qing, SANG Xian-Chun, ZHAO Fang-Ming, LI Yun-Feng, LING Ying-Hua,YANG Zheng-Lin,HE Guang-Hua*   

  1. Rice Research Institute of Southwest University / Chongqing Key Laboratroy of Application and Safety Control of Genetically Modified Crops / Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing, 4007145, China
  • Received:2011-07-13 Revised:2011-10-15 Published:2012-03-12 Published online:2012-01-04
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

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1303

被引次数

9

摘要： 叶片形态是理想株型的重要指标之一，叶片适度卷曲有利于理想株型的建成，是水稻超高产育种的重要材料。在EMS诱变籼稻缙恢10号群体中发现一个卷叶突变体，表现叶片筒状卷曲，经过多代连续自交，性状稳定，命名为rl13 (rolled leaf 13)。rl13的叶绿素a、叶绿素b和总叶绿素含量均显著高于野生型对照缙恢10号，类胡萝卜素含量在苗期、孕穗期与野生型相比有显著提高，而抽穗期和成熟期则差异不显著。rl13的三片功能叶的卷曲度与野生型相比均达到极显著差异，但rl13的三片功能叶之间差异不显著。通过石蜡切片分析，突变体叶肉细胞层数变薄，野生型含有的一个较大泡状细胞转变为卷叶突变体的两个大小相近的泡状细胞，导致了叶片弯曲。以该突变体为父本，西农1A为母本配制杂交组合构建F2遗传群体，结果表明，该卷叶性状由一对隐性核基因控制。选用F2代分离群体中的1 215个隐性单株作为定位群体，将RL13定位在第6染色体短臂上分子标记RM276和SWU6-1之间，遗传距离分别为1.1 cM和0.2 cM。

关键词: 水稻(Oryza sativa L.), 卷叶突变体rl13, 遗传分析, 分子定位

Abstract: Rolled 1eaf mutants are good materials rice in breeding for leaf and plant type. A rolled 1eaf mutant rl13 was derived from an EMS-treated mutation in an indica line (Jinhui 10). The chlorophyll content of rl13 was significantly higher than that of wild type Jinhui10. There was a highly significant difference in the curl of the three functional mutant leaves compared with those with wild-type. However, there were no significant differences between the three functional mutants. Through the analysis of paraffin, mesophyll cells per layer in rl13 thinned, wild-type cells contain a large bubble into two similar size vesicular cells of mutants, which resulted in the character of rolled leaf. We made the combination of Xinong1A/rl13 to establish genetic population for genetic analysis, the results confirmed that the mutant trait was controlled by a single recessive nuclear gene. In the F2, the gene of the rolled leaf trait was mapped between two SSR markers, RM276 and SWU6-1, with distances of 1.1 cM and 0.2 cM, respectively.

Key words: Rice (Oryza sativa L.), Rolled leaf mutant, Genetic analysis, Molecular mapping

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