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作物学报 ›› 2013, Vol. 39 ›› Issue (06): 1030-1038.doi: 10.3724/SP.J.1006.2013.01030

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

豌豆抗白粉病资源筛选及分子鉴定

王仲怡1,包世英2,段灿星1,宗绪晓1,朱振东1,*   

  1. 1 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程,北京100081;2云南省农业科学院粮食作物研究所,云南昆明 650205
  • 收稿日期:2012-11-19 修回日期:2013-01-15 出版日期:2013-06-12 网络出版日期:2013-03-22
  • 通讯作者: 朱振东, E-mail: zhuzhendong@caas.cn, Tel: 010-82109609
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-09)和农业部作物种质资源保护子项目(NB2010-2130135-25-14)资助。

Screening and Molecular Identification of Resistance to Powdery Mildew in Pea Germplasm

WANG Zhong-Yi1,BAO Shi-Ying2,DUAN Can-Xing1,ZONG Xu-Xiao1,ZHU Zhen-Dong1,*   

  1. 1 Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Institute of Grain Crops, Yunnan Academy of Agricultural Sciences, Kunming 650205, China
  • Received:2012-11-19 Revised:2013-01-15 Published:2013-06-12 Published online:2013-03-22
  • Contact: 朱振东, E-mail: zhuzhendong@caas.cn, Tel: 010-82109609

摘要:

由豌豆白粉菌引起的白粉病是豌豆生产上的重要病害,利用抗病品种是防治该病害最经济有效的方法。本研究在控制条件下苗期接种鉴定了396份豌豆资源对2个不同地理来源的豌豆白粉病菌分离物EPBJEPYN的抗性,用4个与豌豆抗白粉病基因er1连锁的SCAR标记对66份免疫或抗病资源进行标记基因型鉴定。结果表明,在鉴定的396份资源中,有101份资源表现免疫或抗病,其中对分离物EPBJEPYN免疫的资源分别为59(14.9%)60(15.2%),对2个分离物均免疫的资源有54(13.6%);在鉴定的82份中国资源中,有8份对2个分离物均表现免疫。分子标记将66份免疫或抗病资源鉴定为13个标记基因型,同一地理来源的抗性资源分属不同的标记基因型,其中8份来自中国云南的抗性资源分属7个标记基因型。研究表明,中国存在有效的豌豆白粉病抗源,抗性资源具有丰富的遗传多样性。

关键词: 豌豆, 白粉病, 抗病基因, 分子鉴定

Abstract:

Pea powdery mildew, caused by Erysiphe pisi, is one of the most important diseases in pea production. The utilization of resistant pea cultivars is the most economical and effective method for controlling the disease. In this study, Three hundred and ninety-six pea accessions were evaluated for resistance to two powdery mildew isolates from different geographical origins in seedling stage under controlling condition, and four SCAR makers closely linked to pea powdery mildew resistance gene er1 were used to genotype accessions with immunity or resistance. The results showed that 101 accessions were immune or resistant to powdery mildew, 59 (14.9%) and 60 (15.2%) accessions were immune to the Beijing isolate and Yunnan isolate, respectively, 54 (13.6%) accessions were immune to both the Beijing isolate and Yunnan isolate. In eighty-two accessions from China, only eight were immune to both of the two isolates. Thirteen marker genotypes were identified in 66 selected accessions with immunity or resistance through molecular genotyping, and the accessions from the same geographical origin contained different marker genotypes. Eight accessions from Yunnan Province of China shared seven marker genotypes. The above results indicated that pea germplasm with resistance to pea powdery mildew has effective and extensive genetic diversity in China.

Key words: Pisum sativum, Powdery Mildew, Resistance gene, Molecular identification

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