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作物学报 ›› 2014, Vol. 40 ›› Issue (05): 805-815.doi: 10.3724/SP.J.1006.2014.00805

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

河南省小麦主推品种对两种禾谷孢囊线虫的抗性及其评价方法

邢小萍,袁虹霞,孙君伟,张洁,孙炳剑,李洪连*   

  1. 河南农业大学植物保护学院,河南郑州 450002
  • 收稿日期:2013-08-02 修回日期:2014-01-12 出版日期:2014-05-12 网络出版日期:2014-02-14
  • 通讯作者: 李洪连, E-mail: honglianli@sina.com, Tel: 0371-63558791
  • 作者简介:李洪连, E-mail: honglianli@sina.com, Tel: 0371-63558791
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项(200903040),国家高技术研究发展计划(863计划)项目(2012AA101105),国家“十二五”粮食丰产科技工程(2012BAD04B07)和中国科学院重点部署项目(CXJQ120111)资助。

Resistance to Two Species of Cereal Cyst Nematode and Its Evaluation Methods in 47 Major Wheat Cultivars in Henan Province, China

XING Xiao-Ping,YUAN Hong-Xia,SUN Jun-Wei,ZHANG Jie,SUN Bing-Jian,LI Hong-Lian*   

  1. College of Plant Protection, Henan Agriculture University, Zhengzhou 450002, China
  • Received:2013-08-02 Revised:2014-01-12 Published:2014-05-12 Published online:2014-02-14
  • Contact: 李洪连, E-mail: honglianli@sina.com, Tel: 0371-63558791
  • About author:李洪连, E-mail: honglianli@sina.com, Tel: 0371-63558791

摘要:

通过室内人工接种和田间自然病圃鉴定,以单株白雌虫数法、相对抗病指数法和繁殖系数法评价河南省47个主推的小麦品种对燕麦孢囊线虫Heterodera avenae荥阳群体和菲利普孢囊线虫H. filipjevi许昌群体的抗性。室内人工接种条件下,依据单株白雌虫数评价,所有小麦品种对两种孢囊线虫均表现感病或高度感病;但依据相对抗病指数评价,有4个品种(太空6号、新麦11、中育6号和新麦18)H. avenae表现抗病,其余品种表现中度以上感病。田间病圃鉴定条件下,依据单株白雌虫数评价,太空6号和新麦18H. avenae表现高抗,中育6号、新麦1110个品种表现中抗,其余品种均表现为感病或高感;中育6号和太空6号对H. filipjevi表现高抗,偃展4110、濮麦9号和豫农201表现中抗,其余品种均表现感病。依据相对抗病指数评价,太空6号对H. avenae表现高抗,新麦18、中育6号和新麦11表现抗病;中育6号、太空6号对H. filipjevi表现高抗,偃展4110、濮麦9号、豫农201、豫农949表现抗病。依据繁殖系数Pf /Pi评价,太空6号、新麦11、中育6号和新麦18H. avenae表现抗病,太空6号、中育6号、濮麦9号和濮优938H. filipjevi表现抗病,其余品种表现感病。3种方法的抗性评价结果不完全相同,相对抗病指数法与单株白雌虫数量法的评价结果在一定程度上有一致性,可部分克服因感病程度悬殊而导致评价不一致的问题,因而可作为小麦品种抗禾谷孢囊线虫新的鉴定方法。

关键词: 禾谷孢囊线虫, 相对抗病指数, 繁殖系数(Pf /Pi比), 抗性评价方法

Abstract:

Resistance to cereal cyst nematode (CCN) is an important breeding target in wheat. In this study, we evaluated the resistance to CCN Xingyang population (Heterodera avenae) and Xuchang population (H. filipjevi) in 47 wheat cultivars from Henan Province, China through greenhouse and field tests. The CCN resistance was evaluated by the methods of average number of white female per plant, relative resistance index (RRI) and Pf /Pi ratio. All cultivars were susceptible to H. avenae and H. filipjevi evaluated by average number of white female per plant after artificial inoculation in greenhouse. However, Taikong 6, Xinmai 11, Zhongyu 6, and Xinmai 18 showed resistance to H. avenae based on RRI evaluation. In field test, when evaluated with average number of white female per plant, there were a few resistant cultivars, including Taikong 6 and Xinmai 18 with high resistance and 10 cultivars (such as Zhongyu 6 and Xinmai 11) with moderate resistance to H. avenae, and Zhongyu 6 and Taikong 6 with high resistance and three cultivars (Yanzhan 4110, Pumai 9, and Yunong 201) with moderate resistance to H. filipjevi. The result of RRI evaluation showed that Taikong 6 was highly resistant to H. avenae, and Xinmai 18, Zhongyu 6 and Xinmai 11 were resistant to H. avenae; Zhongyu 6 and Taikong 6 were highly resistant to H. filipjevi and 4 cultivars (Yanzhan 4110, Pumai 9, Yunong 201, and Yunong 949) were resistant to H. filipjevi. Using Pf /Piratio as the evaluation index, four cultivars (Taikong 6, Xinmai 11, Zhongyu 6, and Xinmai 18) were resistant to H. avenae, and four cultivars (Taikong 6, Zhongyu 6, Pumai 9, and Puyou 938) were resistant to H. filipjevi. The resistance evaluation based on RRI method was partially identical to that based on white female number per plant method, and RRI method has the advantage to reduce the great evaluation error among susceptible cultivars. This method can be considered in CCN resistance evaluation in wheat.

Key words: Cereal cyst nematode, Relative resistance index, Pf /Pi ratio, Methods for resistance evaluation

[1]Nicol J M, Turner S J, Coyne D L, den Nijs L, Hockland S, Maafi Z T. Current nematode threats to world agriculture. In: Jones J, Gheysen G, Fenoll C, eds. Genomics and Molecular Genetics of Plant-nematode Interactions. Dordrecht: Springer, 2011. pp 21–43



[2]Nicol J M, Rivoal R. Global knowledge and its application for the integrated control and management of nematodes on wheat. In: Ciancio A, Mukerji K G, eds. Integrated Management and Biocontrol of Vegetable and Grain Crops Nematodes. Dordrecht: Springer, 2008. pp 243–287



[3]Rivoal R, Nicol J M. Past research on the cereal cyst nematode complex and future needs. In: Riley I T, Nicol J M, Dababat A A, eds. Cereal cyst nematodes: status, research and outlook. Ankara, Turkey: CIMMYT, 2009. pp 3–10



[4]Handoo Z A. A key and compendium to species of the Heterodera avenae group (Nematoda: Heteroderidae). J Nematol, 2002, 34: 250–262



[5]陈品三, 王明祖, 彭德良. 我国小麦禾谷孢囊线虫(Heterodera avenae Wollenweber)的发现与鉴定初报. 中国农业科学, 1991, 24(5): 89–91



Chen P S, Wang M Z, Peng D L. Preliminary report of identification on cereal cyst nematode of wheat in China. Sci Agric Sin, 1991, 24(5): 89–91 (in Chinese with English abstract)



[6]彭德良, 李惠霞, 王锡锋, Relay I, Nicol J, 侯生英, 欧师琪. 我国小麦禾谷孢囊线虫的新发生分布地区. 见: 廖金铃, 彭德良, 段玉玺主编. 中国线虫学研究(第二卷). 北京: 中国农业科技出版社, 2008. pp 344–345



Peng D L, Li H X, Wang X F, Relay I, Nicol J, Hou S Y, Ou S Q. New distribution of cereal cyst nematode (Heterodera avenae) in China. In: Liao J L, Peng D L, Duan Y X, eds. Nematology Research in China. Vol. 2. Beijing: China Agricultural Science and Technology Press, 2008. pp 344–345 (in Chinese)



[7]Peng D L, Nicol J M, Li H M, Hou S Y, Li H X, Chen S L, Dababat A A. Current knowledge of cereal cyst nematode (Heterodera avenae) on wheat in China. In: Riley I T, Nicol J M, Dababat A A, eds. Cereal cyst nematodes: status, research and outlook. Ankara, Turkey: CIMMYT, 2009. pp 21–23



[8]黄文坤, 叶文兴, 王高峰, 龙海波, 欧师琪, 彭德良. 宁夏地区禾谷孢囊线虫的发生与分布. 华中农业大学学报, 2011, 30: 74–77



Huang W K, Ye W X, Wang G F, Long H B, Ou S Q, Peng D L. Occurrence and distribution of Heterodera avenae Wollenweber in Ningxia Hui Autonomous region. J Huazhong Agric Univ, 2011, 30: 74–77 (in Chinese with English abstract)



[9]李惠霞, 柳永娥, 魏庄, 李敏权. 新疆和西藏发现禾谷孢囊线虫. 见: 廖金铃, 彭德良, 段玉玺, 简恒, 李红梅主编. 中国线虫学研究(第四卷). 北京: 中国农业科技出版社, 2012. pp 163–165



Li H X, Liu Y Z, Wei Z, Li M Q. The detection of Heterodera avenae from the cereal field in autonomous regions of Tibet and Xinjiang. In: Liao J L, Peng D L, Duan Y X, Jian H, Li H M, eds. Nematology research in China Vol. 4. Beijing: China Agricultural Science and Technology Press, 2012. pp 163–165 (in Chinese)



[10]彭德良, 黄文坤, 孙建华, 刘崇俊, 张辉民. 我国天津发现小麦禾谷孢囊线虫. 见: 廖金铃, 彭德良, 段玉玺, 简恒, 李红梅主编. 中国线虫学研究(第四卷). 北京: 中国农业科技出版社, 2012. pp 162–163



Peng D L, HuangW K, Sun J H, Liu C J, Zhang H M. First report of cereal cyst nematode (Heterodera avenae) in Tianjing,China. In: Liao J L, Peng D L, Duan Y X, Jian H, Li H M, eds. Nematology research in China Vol.4. Beijing: China Agricultural Science and Technology Press, 2012. pp 162–163 (in Chinese)



[11]Peng D L, Ye W X, Peng H, Gu X C. First report of the cyst nematode (Heterodera filipjevi) on wheat in Henan Province. Plant Dis, 2010, 94: 1262



[12]Li H L, Yuan H X, Sun J W, Fu B, Nian G L, Hou X S, Xing X P, Sun B J. First record of the cereal cyst nematode Heterodera filipjevi in China. Plant Dis, 2010, 94: 1505



[13]Li H L, Yuan H X, Wu X J, Yang W X, Nicol J. Cereal cyst nematode (Heterodera avenae) is causing damage on wheat in Henan: the bread basket of China. Proceedings of the 16th international plant protection congress. Glasgow, Scotland, UK: BCPC Press, 2007. pp 674–675



[14]宗莹莹, 代君丽, 袁虹霞, 邢小萍, 孙炳剑, 李洪连. 普通小麦品种中育6号对两种禾谷孢囊线虫的抗性遗传分析. 麦类作物学报, 2013, 33: 249-254



Zong Y Y, Dai J L, Yuan H X, Xing X P, Sun B J, Li H L. Genetic analysis of resistance to cereal cyst nematode in common wheat variety Zhongyu 6. J Triticeae Crops, 2013, 33: 249–254 (in Chinese with English abstract)



[15]Hajihasani A, Nicol J M, Rezaee S, Tanha M Z. Effect of the cereal cyst nematode, Heterodera filipjevi, on wheat in microplot trials. Nematology, 2010, 12: 357–363



[16]Hajihasani A, Tanha M Z, Nicol J M, Seraji A. Relationships between population densities of the cereal cyst nematode, Heterodera latipons and yield losses of winter wheat in microplots. Aust Plant Pathol, 2010, 39: 530–535



[17]Nicol J M, Rivoal R. Integrated management and biocontrol of vegetable and grain crops nematodes. In: Ciancio A, Mukerji K G, eds. Global Knowledge and Its Application for the Integrated Control and Management of Nematodes on Wheat. The Netherlands: Springer, 2007. pp 243–287



[18]Brown R H. Ecology and control of cereal cyst nematode (Heterodera avenae) in southern Australia. J Nematol, 1984, 16: 216-222



[19]Taya A S, Dalal M R, Rana B P. The effect of cropping sequence on cereal cyst nematode Heterodera avenae and wheat crops. Agric Sci Digest, 2000, 20: 275-276



[20]Eastwood R F, Lagudah E S, Appels R, Hannah M, Kollmorgen J F. Triticum tauschii: a novel source of resistance to cereal cyst nematode (Heterodera avenae). Aust J Agric Res, 1991, 42: 69–77



[21]Rathjen A J, Eastwood R F, Lewis J G, Dube A J. Breeding wheat for resistance to Heterodera avenae in southeastern Australia. Euphytica, 1998, 100: 55–62



[22]王振跃, 高书峰, 李洪连, 沙广乐, 余懋群. 不同小麦品种(系)对禾谷孢囊线虫病的抗性鉴定. 河南农业科学, 2006, (5): 50–52



Wang Z Y, Gao S F, Li H L, Sha G L, Yu M Q. Resistance of different wheat cultivars to cereal cyst nematode. J Henan Agric Sci, 2006, (5): 50–52 (in Chinese with English abstract)



[23]郑经武, 林茂松, 程瑚瑞, 方中达. 麦类作物对禾谷胞囊线虫的抗病性. 植物保护学报, 1999, 26: 250–254



Zheng J W, Lin M S, Cheng H R, Fang Z D. Resistance of cereal cultivars to cereal cyst nematode, Heterodera avenae. Acta Phytophyl Sin, 1999, 26: 250–254 (in Chinese with English abstract)



[24]刘炳良, 孙成刚, 王暄, 向桂林, 宋志强, 高菲菲, 李红梅. 小麦品种对禾谷孢囊线虫(Heterodera avenae)江苏沛县群体的抗性鉴定. 麦类作物学报, 2012, 32: 563–568



Liu B L, Sun C G, Wang X, Xiang G L, Song Z Q, Gao F F, Li H M. Evaluation of the resistance of wheat cultivars to Peixian population of Heterodera avenae from Jiangsu Province, China. J Triticeae Crops, 2012, 32: 563–568 (in Chinese with English abstract)



[25]赵洪海, 杨远永, 彭德良. 山东省主要小麦品种对禾谷孢囊线虫抗性的初步评价. 山东农业科学, 2012, 44(2): 80–83



Zhao H H, Yang Y Y, Peng D L. Preliminary evaluation on resistance of main wheat cultivars from Shandong Province to cereal cyst nematode. Shandong Agric Sci, 2012, 44(2): 80–83 (in Chinese with English abstract)



[26]刘静, 吴海燕, 彭德良. 山东省主栽小麦品种对孢囊线虫(Heterodera avenae)抗性的鉴定. 作物杂志, 2012, (1): 111–114



Liu J, Wu H Y, Peng D L. Evaluation of the main wheat varieties from Shandong for resistance to Heterodera avenae. Crops, 2012, (1): 111–114 (in Chinese with English abstract)



[27]Fisher J M. Towards a consistent laboratory assay for resistance to Heterodera avenae. EPPO Bull, 1982, 12: 445–449



[28]Lewis J G, Matic M, Mckay A C. Success of cereal cyst nematode resistance in Australia: History and status of resistance screening systems. In: Riley I T, Nicol J M, Dababat A A, eds. Cereal Cyst Nematodes: Status, Research and Outlook. Ankara, Turkey: International Maize and Wheat Improvement Centre (CIMMYT) Press, 2009. pp 137–142



[29]Zoriano I R, Schmit V, Brar D S, Prot J C, Reversat G. Resistance to rice root-knot nematode Meloidogyne graminicola identified in Oryza longistaminata and O. glaberrima. Nematology, 1999, 1: 395–398



[30]李洪连, 袁虹霞, 刁晓葛, 李锁平, 胡玉欣, 王守正. 河南小麦主要品种纹枯病抗性评价. 河南农业大学学报, 1998, 32: 107–111



Li H L, Yuan H X, Diao X G, Li X P, Hu Y X, Wang S Z. Evaluation on the resistance of major wheat varieties in Henan Province to sharp eyespot. Acta Agric Univ Henanensis, 1998, 32: 107–111 (in Chinese with English abstract)



[31]杨爱国, 潘光堂, 叶华智, 唐莉, 荣廷昭. 玉米自交系纹枯病抗性鉴定及抗病资源筛选. 植物保护, 2003, 29(1): 25–28



    Yang A G, Pan G T, Ye H Z, Tang L, Rong T Z. Evaluating resistance of inbred lines of corn to maize sheath blight and screening of resistance resources. Plant Prot, 2003, 29(1): 25–28 (in Chinese with English abstract)



[32]杨立军, 杨小军, 喻大昭, 赵永玉. 小麦品种纹枯病抗性鉴定. 华中农业大学学报, 2001, 20(2): 122–124



Yang L J, Yang X J, Yu D Z, Zhao Y Y. Evaluation of wheat cultivar resistance to sharp eyespot. J Huazhong Agric Univ, 2001, 20(2): 122–124 (in Chinese with English abstract)



[33]张明, 张大乐, 苏亚蕊, 李锁平. 粗山羊草(Aegilops tauschii) 抗纹枯病的鉴定. 河南大学学报(自然科学版), 2010, 40(1): 58–61



Zhang M, Zhang D L, Su Y R, Li S P. Evaluation on the resistance to sharp eyespot of Aegilops tauschii. J Henan Univ (Nat Sci), 2010, 40(1): 58–61 (in Chinese with English abstract)



[34]袁虹霞, 张福霞, 张佳佳, 侯兴松, 李洪杰, 李洪连. CIMMYT小麦种质资源对菲利普孢囊线虫(Heterodera filipjevi)河南许昌群体的抗性. 作物学报, 2011, 37: 1956–1966



Yuan H X, Zhang F X, Zhang J J, Hou X S, Li H J, Li H L. Resistance of CIMMYT wheat germplasm to Heterodera filipjevi Xuchang population from Henan Province, China. Acta Agron Sin, 2011, 37: 1956–1966(in Chinese with English abstract)



[35]Stirling G, Nicol J M, Reay F. Advisory services for nematode pests. Operational guidelines. Rural Industries Research and Development Corporation Publication, Caberra, ACT, Australia, 1999, 41: 111



[36]Yuan H X, Sun J W, Yang W X, Xing X P, Wang Z Y, Riley I T, Li H L. New pathotypes of Heterodera avenae (cereal cyst nematode) from winter wheat in Zhengzhou, Henan, China. Aust Plant Pathol, 2010, 39: 107–111



[37]张佳佳, 袁虹霞, 张瑞奇, 邢小萍, 代君丽, 牛吉山, 李洪连, 陈佩度. 普通小麦-簇毛麦种质对菲利普孢囊线虫的抗性分析. 作物学报, 2012, 38: 1969–1976



Zhang J J, Yuan H X, Zhang R Q, Xing X P, Dai J L, Niu J S, Li H L, Chen P D. Analysis of resistance to Heterodera filipjevi in Triticum aestivum–Dasypyrum villosum germplasm. Acta Agron Sin, 2012, 38: 1969–1976 (in Chinese with English abstract)



[38]Li H J, Cui L, Li H L, Wang X M, Murray T D, Conner R L, Wang L J, Gao X, Sun Y, Sun S C, Tang W H. Effective resources in wheat and wheat–derivatives for resistance to Heterodera filipjevi in China. Crop Sci, 2012, 52: 1209–1217



[39]Nicol J M, Necmett N B, Yildirim A F, Yorgancilar A, Kilin A T, Elekcioglu I H, Sahin E, Erginbas-Orakci G, Braun H J. Identification of genetic resistance to cereal cyst nematode (Heterodera filipjevi) for international bread wheat improvement. In: Riley I T, Nicol J M, Dababat A A, eds. Cereal Cyst Nematodes: Status, Research and Outlook. Ankara, Turkey: International Maize and Wheat Improvement Centre (CIMMYT) press, 2009. pp 160–165

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