欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2012, Vol. 38 ›› Issue (04): 571-577.doi: 10.3724/SP.J.1006.2012.00571

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

硬粒小麦品种Waskana和Waskowa对禾谷孢囊线虫(Heterodera filipjevi和H. avenae)的抗性

高秀1,2，崔磊2,3，李洪连3，王晓鸣2，唐文华4，Robert L. CONNER5，林小虎1,*，李洪杰2,*   

  1. 1 河北科技师范学院生命科技学院，河北秦皇岛 066004；2 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程，北京100081；3 河南农业大学植物保护学院，河南郑州450002；4 中国农业大学植物病理系，北京100193；5 Agriculture and Agri-Food Canada, Morden Research Station, Unit 100-101 Route 100, Morden, MB R6M 1Y5 Canada
  • 收稿日期:2011-11-03 修回日期:2012-01-19 出版日期:2012-04-12 网络出版日期:2012-02-14
  • 通讯作者: 李洪杰, E-mail: hongjie@caas.net.cn; 林小虎, E-mail: xiaohulin2008@163.com
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项(200903040), 国家重点基础研究计划(973计划)项目(2009CB118300), 科技部中澳特别基金项目(2010DFA31380), 国家现代农业产业体系建设项目(CARS-3-1)和国家自然科学基金项目(31171545)资助。

Resistance of Triticum durum Cultivars Waskana and Waskowa to Cereal Cyst Nematode, Heterodera filipjevi and H. avenae

GAO Xiu1,2,CUI Lei2,3,LI Hong-Lian3,WANG Xiao-Ming2,TANG Wen-Hua4,Robert L. CONNER5,LIN Xiao-Hu1,*,LI Hong-Jie2,*   

  1. 1 Hebei Normal University of Science and Technology, Qinhuangdao 066004, China; 2 Institute of Crop Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China; 4 Department of Plant Pathology, China Agricultural University, Beijing 100193, China; 5 Agriculture and Agri-Food Canada, Morden Research Station, Unit 100-101 Route 100, Morden, MB R6M 1Y5 Canada
  • Received:2011-11-03 Revised:2012-01-19 Published:2012-04-12 Published online:2012-02-14
  • Contact: 李洪杰, E-mail: hongjie@caas.net.cn; 林小虎, E-mail: xiaohulin2008@163.com

PDF

1553

被引次数

13

摘要： 禾谷孢囊线虫(cereal cyst nematode, CCN)是一类重要的土传小麦病原线虫，危害我国小麦的主要是燕麦孢囊线虫(Heterodera avenae)和菲利普孢囊线虫(H. filipjevi)。我国对这些病原线虫的抗性资源十分缺乏，寻找新抗源是当前抗性育种的重要工作。本研究通过3年的田间病圃和温室接种鉴定，发现加拿大的硬粒小麦品种Waskana和Waskowa对H. filipjevi (河南许昌群体，Hfc-1致病型)和H. avenae (河南荥阳群体，Ha43致病型)都表现很强的抗性，单株孢囊数显著少于感病的普通小麦品种矮抗58、石4185和温麦19。显微观察可见，虽然两种线虫的幼虫都能够侵入Waskana和Waskowa的根组织内，但是根内的线虫数量显著少于感病对照普通小麦品种，最终在根系上形成的可见孢囊数量也较少。Waskana和Waskowa对两种病原线虫的抗性为我国抗CCN小麦品种选育提供了有较高利用价值的新抗源。根据南澳大利亚研究所的土传病害检测服务系统对土壤中病原线虫的分子检测结果，抗CCN品种Waskana和Waskowa根际土壤中的线虫虫卵量低于感病小麦品种，因此种植可能降低土壤中禾谷孢囊线虫危害的风险。

关键词: 硬粒小麦, 禾谷孢囊线虫, Heterodera filipjevi, H. avenae, 抗病性

Abstract: Cereal cyst nematodes (CCN) are a group of important soil-borne pathogens of wheat. Heterodera avenae and H. filipjevi are the major species of CCN that have limited wheat (Triticum aestivum) production in China in recent years. Since the CCN-resistant resource is short, it is important to develop new sources with resistance for breeding purpose in China. Based on the results of a 3-year field test and controlled environment tests, we found that two Canadian Triticum durum cultivars, Waskana and Waskowa, were highly resistant to both Heterodera filipjevi (pathotype Hfc-1) and H. avenae (pathotype HA43) populations from Henan Province, China. The number of white females per plant in Waskana and Waskowa was significantly smaller than that in the susceptible common wheat cultivars Aikang 58, Shi 4185, and Wenmai 19. Although the juveniles of H. filipjevi and H. avenae populations were able to penetrate into the roots of Waskana and Waskowa, the number of juveniles inside the roots was significantly smaller than that of Aikang 58, Shi 4185, and Wenmai 19, resulting in smaller numbers of females on the roots of these durum wheat cultivars. Waskana and Waskowa with resistance to H. filipjevi and H. avenae are useful in developing wheat cultivars with enhanced resistance to CCN in China. Based on the results from the DNA-based soil testing service operated by South Australian Research and Development Institute, the number of eggs of nematodes in the rhizospheric soil samples from the CCN-resistant cultivars Waskana and Waskowa was less than that in the soil samples from the susceptible wheat cultivars. This indicates that application of resistant cultivars might reduce the risk of damage caused by CCN in soil.

Key words: Triticum durum, Cereal cyst nematode (CCN), Heterodera filipjevi, H. avenae, Resistance

[1] Chen P-S(陈品三), Wang M-Z(王明祖), Peng D-L(彭德良). Preliminary report of identification on cereal cyst nematode of wheat in China. Sci Agric Sin(中国农业科学), 1991, 24(5): 89-91 (in Chinese with English abstract)
[2] Peng D L, Nicol J M, Li H M, Hou S Y, Li H X, Chen S L, Ma P, Li H L, Riley I T. Current knowledge of cereal cyst nematode (Heterodera avenae) on wheat in China. In: Riley I T, Nicol J M, Dababat A A, eds. Cereal Cyst Nematodes: Status, Research and Outlook. Ankara, Turkey: CIMMYT Press, 2009. pp 29-34
[3] Handoo Z A. A key and compendium to species of the Heterodera avenae group (Nematoda: Heteroderidae). J Nematol, 34: 250-202
[4] Peng D L, Ye W X, Peng H, Gu X C. First report of the cyst nematode (Heterodera filipjevi) on wheat in Henan Province, China. Plant Dis, 2010, 94: 1262
[5] Li H L, Yuan H X, Sun J W, Fu B, Nian G L, Hou X S, Xing X P, Sun B J. First record of the cereal cyst nematode Heterodera filipjevi in China. Plant Dis, 2010, 94: 1505
[6] Rathjen A J, Eastwood R F, Lewis J G, Dube A J. Breeding wheat for resistance to Heterodera avenae in Southeastern Australia. Euphytica, 1998, 100: 55-62
[7] Li H J, Cui L, Li H L, Wang X M, Murray T D, Conner R L, Wang L J, Gao X, Sun Y, Sun S C, Tang W H. Effective resources of wheat and wheat-Thinopyrum derivatives for resistance to Heterodera filipjevi in China. Crop Sci, 2012, doi: 10.2135/cropsci2011.11.0591
[8] Wang Z-Y(王振跃), Gao S-F(高书峰), Li H-L(李洪连), Sha G-L(沙广乐), Yu M-Q(余懋群). Resistance of different wheat cultivars to cereal cyst nematode. J Henan Agric Sci (河南农业科学), 2006, (5): 50-52 (in Chinese with English abstract)
[9] Zheng J-W(郑经武), Lin M-S(林茂松), Cheng H-R(程瑚瑞), Fang Z-D(方中达). Resistance of cereal cultivars to cereal cyst nematode, Heterodera avenae. Acta Phytophylacica Sin (植物保护学报), 1999, 26(3): 250-254 (in Chinese with English abstract)
[10] Yuan H-X(袁虹霞), Zhang F-X(张福霞), Zhang J-J(张佳佳), Hou X-S(侯兴松), Li H-J(李洪杰), Li H-L(李洪连). Resistance of CIMMYT wheat germplasm to Heterodera filipjevi Xuchang population from Henan Province, China. Acta Agron Sin作物学报), 2011, 37(11): 1956-1966 (in Chinese with English abstract) (
[11] Bekal S, Jahier J, Rivoal R. Host responses of Triticeae to species of the cereal cyst nematode complex in relation to breeding resistant durum wheat. Fundam Appl Nematol, 1998, 21: 359-370
[12] Nicol J M, Bolat N, Yildirim A F, Yorgancilar A, Kilinç A T, Elekçio?lu H I, ?ahin E, Erginba?-Orakçi G, Braun H J. Identification of genetic resistance to cereal cyst nematode (Heterodera filipjevi) for international bread wheat improvement. In: Riley I T, Nicol J M, Dababat A A, eds. Cereal Cyst Nematodes: Status, Research and Outlook. Ankara, Turkey: CIMMYT Press, 2009. pp 160-165
[13] Slootmaker L A, Lange W, Jochemsen G, Schepers J. Monosomic analysis in bread wheat of resistance to cereal root eelworm. Euphytica, 1974, 23: 497-503
[14] Williams K J, Lewis J G, Bogacki P, Pallotta M A, Willsmore K L, Kuchel H, Wallwork H. Mapping of a QTL contributing to cereal cyst nematode tolerance and resistance in wheat. Aust J Agric Res, 2003, 54: 731-737
[15] Delibes A, Romero D, guaded S, Duce A, Mena M, Lopez-Braña I, Andrés M-F, Martin-Sanchez J-A, García-Olmedo F. Resistance to the cereal cyst nematode (Heterodera avenae Woll.) transferred from the wild grass Aegilops ventricosa to hexaploid wheat by a “stepping-stone” procedure. Theor Appl Genet, 1993, 87: 402-408
[16] Jahier J, Tanguy A M, Abelard P, Rivoal R.Utilization of deletions to location a gene for resistance cereal cyst nematode (Heterodera avenae), on an Aegilops ventricosa chromosome. Plant Breed, 1996, 115: 282-284
[17] Ogbonnaya F C, Seah S, Delibes A, Jahier J, López-Braña I, Eastwood R F, Lagudah E S. Molecular-genetic characterisation of a new nematode resistance gene in wheat. Theor Appl Genet, 2001, 102: 623-629

[18] Eastwood R F, Lagudah E S, Appels R. A directed search for DNA sequences tightly linked to cereal cyst nematode resistance genes in Triticum tauschii. Genome, 1994, 37: 311-319
[19] Romero M D, Montes M J, Sin E, López-Braña I, Duce A, Martín-Sánchez, Andrés M F, Delibes A. A cereal cyst nematode (Heterodera avenae Woll.) resistance gene transferred from Aegilops triuncialis to hexaploid wheat. Theor Appl Genet, 1998, 96: 1135-1140


[20] Taylor C, Shepherd K W, Langridge P. A molecular genetic map of the long arm of chromosome 6R of rye incorporating the cereal cyst nematode resistance gene, CreR. Theor Appl Genet, 1998, 97: 1000-1012
[21] Paull J G, Chalmers K J, Karakousis A, Kretschmer J M, Manning S, Langridge P. Genetic diversity in Australian wheat varieties and breeding material based on RFLP data. Theor Appl Genet. 1998, 96: 435-446

[22] Yu M Q, Jahier J, Person-Dedryver F. Chromosomal location of a gene (Rkn-mnl) for resistance to the root-knot nematode transferred into wheat from Aegilops. Plant Breed, 1995, 114: 358-360
[23] Hurd E A, Patterson L A, Mallough D, Townley-Smitit T F, Owen C H. Waskana, a new durum wheat. Can J Plant Sci, 1972, 52: 687-688
[24] Nian G-L(年高磊), Sun J-W(孙君伟), Hou X-S(侯兴松), Fu B(付博), Yuan H-X(袁虹霞), Xing X-P(邢小萍), Li H-L(李洪连). Identification of pathotypes of three populations of Heterodera filipjevi in Henan province. In: Liao J-L(廖金铃), Peng D-L(彭德良), Duan Y-X(段玉玺), eds. Nematology Research in China (中国线虫学研究), Vol. 3. Beijing: China Agricultural Science and Technology Press, 2010. pp 120-133 (in Chinese)
[25] Yuan H X, Sun J W, Yang W X, Xing X P, Wang Z Y, Riley I T, Li H L. New pathotypes of Heterodera avenae (cereal cyst nematode) from winter wheat in Zhengzhou, Henan, China. Aust Plant Pathol, 2010, 39: 107-111
[26] Byrd D W, Kirkpatrick T, Barker K R. An improved technique for clearing and staining plant tissues for the detection for clearing and staining plant tissues for the detection of nematode. J Nematol, 1983, 15: 142-143
[27] Ophel-Keller K, McKay A, Hartley D, Herdina, Curran J. Development of a routine DNA-based testing service for soilborne diseases in Australia. Aust Plant Pathol, 2008, 37: 243-253
[28] Oka Y, G’zel U, Speigel Y, Mor M. Cereal cyst nematodes in Israel, and their biology and control strategies. In: Riley I T, Nicol J M, Dababat A A, eds. Cereal Cyst Nematodes: Status, Research and Outlook. Ankara, Turkey: CIMMYT Press, 2009. pp 118-123
[29] Nicol J M, Ogbonnaya F, Singh A K, Bishnoi S P, Kanwar R S, Li H L, Chen S L, Peng D L, Bolat N, ?ahin E, Elekç?lu? H. Current global knowledge of the usability of cereal cyst nematode resistant bread wheat germplasm through international germplasm exchange and evaluation. In: Riley I T, Nicol J M, Dababat A A, eds. Cereal Cyst Nematodes: Status, Research and Outlook. Ankara, Turkey: CIMMYT Press, 2009. pp 149-153
[30] Nicol J M, Rivoal R. Global knowledge and its application for the integrated control and management of nematodes on wheat. In: Ciancio A, Mukerji K G, eds. Integrated Management and Biocontrol of Vegetable and Grain Crops Nematodes. The Netherlands: Springer Academic Publishing, 2008. pp 243-287
[31] McIntosh R A, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers D J, Appels R, Devos K M. Catalogue of gene symbols for wheat. In: Proc 11th Int Wheat Genet Symp, Brisbane, Queensland, Australia, 2008. pp 24-29
[1] 傅华英, 张婷, 彭文静, 段瑶瑶, 许哲昕, 林艺华, 高三基. 甘蔗新品种(系)苗期白条病人工接种抗性鉴定与评价[J]. 作物学报, 2021, 47(8): 1531-1539.
[2] 段亚梅, 罗贤磊, 陈士强, 高勇, 陈建民, 戴毅. 硬粒小麦-长穗偃麦草附加系、代换系和易位系的创制[J]. 作物学报, 2021, 47(7): 1402-1414.
[3] 张雪翠, 孙素丽, 卢为国, 李海朝, 贾岩岩, 段灿星, 朱振东. 河南大豆新品系抗大豆疫霉根腐病基因鉴定[J]. 作物学报, 2021, 47(2): 275-284.
[4] 李竹, 许莉萍, 苏亚春, 吴期滨, 成伟, 孙婷婷, 高世武. 基于田间表型和Bru1基因检测分析甘蔗褐锈病抗性遗传[J]. 作物学报, 2018, 44(02): 306-312.
[5] 孙喜营,崔磊,孙蕾,孙艳玲,邱丹,邹景伟,武小菲,王晓鸣,李洪杰. 抗禾谷孢囊线虫小麦新种质H3714和H4058的培育与鉴定[J]. 作物学报, 2015, 41(06): 872-880.
[6] 张福彦,尚晓丽,吴培培,宋双,陈锋,崔党群. 硬粒小麦品种Lpx-B1位点等位变异的分子鉴定及其脂肪氧化酶活性[J]. 作物学报, 2014, 40(08): 1364-1370.
[7] 邢小萍,袁虹霞,孙君伟,张洁,孙炳剑,李洪连. 河南省小麦主推品种对两种禾谷孢囊线虫的抗性及其评价方法[J]. 作物学报, 2014, 40(05): 805-815.
[8] 李继发，邓志英，孙福来，关西贞，王延训，田纪春. 小麦新品种“山农20”抗病基因的分子检测[J]. 作物学报, 2014, 40(04): 611-621.
[9] 李洪杰,王晓鸣,陈怀谷,李伟,刘东涛,张会云. 小麦-偃麦草杂种后代及小麦种质资源对纹枯病的抗性[J]. 作物学报, 2013, 39(06): 999-1012.
[10] 代君丽,崔磊,刘珂,宗莹莹,袁虹霞,邢小萍,李洪杰,李洪连. 小麦品种太空6号对Heterodera avenae郑州群体的抗性遗传分析[J]. 作物学报, 2013, 39(04): 642-648.
[11] 李祥晓，王倩，罗生香，何云霞，朱苓华，周永力，黎志康. 黑龙江省稻瘟病菌无毒基因分析及抗病种质资源筛选[J]. 作物学报, 2012, 38(12): 2192-2197.
[12] 崔磊，高秀，王晓鸣，简恒，唐文华，李洪连，李洪杰. 不同抗性小麦根与菲利普孢囊线虫(Heterodera filipjevi)互作的表型特征[J]. 作物学报, 2012, 38(06): 1009-1017.
[13] 袁虹霞, 张福霞, 张佳佳, 侯兴松, 李洪杰, 李洪连. CIMMYT小麦种质资源对菲利普孢囊线虫(Heterodera filipjevi)河南许昌群体的抗性[J]. 作物学报, 2011, 37(11): 1956-1966.
[14] 李洪杰, 王晓鸣, 宋凤景, 伍翠平, 武小菲, 张宁, 周阳, 张学勇. 中国小麦品种对白粉病的抗性反应与抗病基因检测[J]. 作物学报, 2011, 37(06): 943-954.
[15] 李余生, 黄胜东, 杨娟, 王才林. 水稻抗稻曲病数量性状座位及效应分析[J]. 作物学报, 2011, 37(05): 778-783.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2