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作物学报 ›› 2014, Vol. 40 ›› Issue (08): 1364-1370.doi: 10.3724/SP.J.1006.2014.01364

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

硬粒小麦品种Lpx-B1位点等位变异的分子鉴定及其脂肪氧化酶活性

张福彦1,2,**,尚晓丽1,**,吴培培1,宋双1,陈锋1,*,崔党群1,*   

  1. 1 河南农业大学农学院/河南省粮食作物协同创新中心 / 小麦玉米作物学国家重点实验室, 河南郑州 450002; 2 河南省核农学重点实验室 / 河南省科学院同位素研究所有限责任公司, 河南郑州 450015
  • 收稿日期:2013-11-15 修回日期:2014-04-16 出版日期:2014-08-12 网络出版日期:2014-06-03
  • 通讯作者: 陈锋, E-mail: chf0088@gmail.com; 崔党群, E-mail: cdq62@sohu.com
  • 基金资助:

    本研究由国家重点基础研究计划(973计划)项目(2014CB138105), 教育部新世纪优秀人才项目(NCET-13-0776), 河南省高校青年骨干教师资助计划项目(2011GGJS-044)和河南省小麦产业技术体系建设专项资金项目(Z2010-01-04)资助。

Molecular Identification of Alleles on Lpx-B1 locus and Lipoxygenase Activity in Durum Wheat (Triticum turgidum L.)

ZHANG Fu-Yan1,2,**,SHANG Xiao-Li1,**,WU Pei-Pei1,SONG Shuang1,CHEN Feng1,*,CUI Dang-Qun1,*   

  1. 1 College of Agronomy / Collaborative Innovation Center of Henan Grain Crops / National Key Laboratory of Wheat & Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China; 2 Henan Key Laboratory of Nuclear Agricultural Sciences / Isotope Institute Co., Ltd, Henan Academy of Sciences, Zhengzhou 450015, China?
  • Received:2013-11-15 Revised:2014-04-16 Published:2014-08-12 Published online:2014-06-03
  • Contact: 陈锋, E-mail: chf0088@gmail.com; 崔党群, E-mail: cdq62@sohu.com

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摘要：

硬粒小麦籽粒中脂肪氧化酶(LOX)与硬粒小麦面制品的加工品质关系密切相关, 而Lpx-B1位点不同变异类型对LOX活性有重要影响。对来自不同国家和地区的167份硬粒小麦品种的LOX活性进行测定, 并对其Lpx-B1位点不同变异类型进行分子鉴定。不同品种间LOX活性差异明显, 变幅为0.20～7.98 AU min-1 g-1。在Lpx-B1.1位点鉴定出3种等位变异, 分别为Lpx-B1.1a、Lpx-B1.1b和Lpx-B1.1c, 以Lpx-B1.1a所占比例最高(55.1%), 其次为Lpx-B1.1c (37.1%), 而Lpx-B1.1b仅占7.8%; Lpx-B1.2和Lpx-B1.3二者总是互补出现在不同的品种中, 146份品种为Lpx-B1.2型, 其余21份品种均为Lpx-B1.3型, 表明二者可能互为一对等位因子。在Lpx-B1.1位点的3种等位变异类型中, Lpx-B1.1b类型品种的LOX活性显著高于Lpx-B1.1a和Lpx-B1.1c类型品种, 而Lpx-B1.1c类型品种的LOX活性最低。Lpx-B1.3类型品种的LOX活性显著高于Lpx-B1.2类型的品种。参试品种共有6种Lpx-B1基因型组合, 其中Lpx-B1.1b/Lpx-B1.3基因型的LOX活性显著高于其他基因型, 而Lpx-B1.1c/Lpx-B1.2和Lpx-B1.1c/Lpx-B1.3基因型的LOX活性最低。这些观测结果为硬粒小麦品质育种提供了重要信息。

关键词: 硬粒小麦, Lpx-B1位点, 等位变异, 脂肪氧化酶

Abstract:

Lipoxygenase (LOX) has a close relationship to the processing quality of flour products in durum wheat (Triticum turgidum L.). Activity of LOX is greatly influenced by the locus of Lpx-B1. The Lpx-B1 alleles in 167 durum wheat varieties from different countries or regions were identified using gene-specific primers, and the Lpx-B1 genotypes were analyzed based on LOX activity data. The LOX activity varied greatly in the 167 varieties, ranging from 0.20 to 7.98 AU min-1 g-1. On Lpx-B1.1 locus, three alleles (Lpx-B1.1a, Lpx-B1.1b, and Lpx-B1.1c) were identified with frequencies of 55.1%, 7.8%, and 37.1%, respectively. Loci Lpx-B1.2 and Lpx-B1.3 were always reciprocally present in varieties, in which Lpx-B1.2 was presented in 146 varieties and Lpx-B1.3 in the remaining 21 varieties. This result indicates the allelic possibility of Lpx-B1.2 and Lpx-B1.3. In the three Lpx-B1.1 genotypes, the Lpx-B1.1b varieties had significantly higher LOX activity than the Lpx-B1.1a and Lpx-B1.1c varieties, and varieties carrying Lpx-B1.1c allele possessed the lowest LOX activity. The LOX activity in Lpx-B1.3 genotypes was significantly higher than that in Lpx-B1.2 genotypes. A total of six genotypic combinations on the three Lpx-B1 loci in 167 varieties. Among them, Lpx-B1.1b/Lpx-B1.3 combination had the highest LOX activity and combinations of Lpx-B1.1c/Lpx-B1.2 and Lpx-B1.1c/Lpx-B1.3 had the lowest LOX activity. These results are informative to quality breeding of durum wheat.

Key words: Triticum turgidum L., Lpx-B1 locus, Alleles, Lipoxygenase

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