欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2013, Vol. 39 ›› Issue (04): 642-648.doi: 10.3724/SP.J.1006.2013.00642

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦品种太空6号对Heterodera avenae郑州群体的抗性遗传分析

代君丽1,崔磊1,2,刘珂1,2,宗莹莹1,袁虹霞1,邢小萍1,李洪杰2,*,李洪连1,*   

  1. 1 河南农业大学植物保护学院, 河南郑州 450002; 2 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程,，北京 100081
  • 收稿日期:2012-10-19 修回日期:2012-12-16 出版日期:2013-04-12 网络出版日期:2013-01-28
  • 通讯作者: 李洪连, E-mail: honglianli@sina.com; 李洪杰, E-mail: hongjie@caas.net.cn
  • 基金资助:

    本研究由国家自然科学基金项目(30971899), 国家公益性行业(农业)科研专项基金(200903040)和“十二五”农村领域国家科技计划课题(2012AA101105)资助。

Genetic Analysis of Common Wheat Cultivar Taikong 6 for Resistance to Heterodera avenae Zhengzhou Population

DAI Jun-Li1,CUI Lei1,2,LIU Ke1,2,ZONG Ying-Ying1,YUAN Hong-Xia1,XING Xiao-Ping1,LI Hong-Jie2,*,LI Hong-Lian1,*   

  1. 1 College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China
  • Received:2012-10-19 Revised:2012-12-16 Published:2013-04-12 Published online:2013-01-28
  • Contact: 李洪连, E-mail: honglianli@sina.com; 李洪杰, E-mail: hongjie@caas.net.cn

RichHTML

PDF

1237

被引次数

5

摘要：

通过多年接虫和田间病圃抗性鉴定，发现普通小麦品种太空6号对燕麦孢囊线虫(Heterodera avenae)郑州群体具有较好的抗性。以抗病品种太空6号为父本、感病品种豫麦47为母本配置杂交组合构建遗传分离群体，通过田间病圃鉴定和室内接虫鉴定对太空6号进行了抗性遗传分析，发现豫麦47×太空6号杂种F2代单株孢囊量为连续分布，具有数量性状特征；F2代群体单株孢囊量分布图呈偏态分布，说明存在明显的主效基因。应用植物数量性状主基因+多基因混合遗传模型单个分离世代分析方法，分析豫麦47×太空6号杂种F2代群体在室内接种二龄幼虫和田间病圃中对H. avenae郑州群体的抗性遗传效应均符合B-2模型，推测太空6号对H. avenae的抗性由2对主基因+多基因控制，主基因表现为加性-显性效应，在室内人工接种和田间病圃鉴定条件下主基因的遗传率分别为73.54%和86.90%，说明太空6号对燕麦孢囊线虫郑州群体的抗性是主效基因起主要作用。

关键词: 太空6号, 小麦禾谷孢囊线虫, Heterodera avenae郑州群体, 抗病性, 遗传分析

Abstract:

Taikong 6 is a common wheat (Triticum aestivum L.) cultivar with high-yield and good agronomic performance bred by Henan Academy of Agricultural Sciences with Satellite-loaded mutation breeding. This cultivar was shown to be resistant to H. avenaee Zhengzhou population in the conditions of repeated inoculation and field tests. In this study, Taikong 6 was crossed with the susceptible cultivarsYumai 47 to produce F2 segregating population. Reactions of the F2 progeny to H. avenaee Zhengzhou population was investigated in the field and inoculation tests. The results of genetic analysis showed that the cyst number of F2 population was the quantitative character with a continuous and skewed distribution, indicating that there are major genes in Taikong 6. The method of joint segregation analysis of single generation of major gene plus polygene mixed inheritance model was used to analyze the inheritance of resistance to H. avenaee Zhengzhou population in Taikong 6, showing that the genetic model B-2 was the most fitted genetic model for the resistance for the cross of Yumai 47 × Taikong 6 in the conditions of inoculation and field tests. This indicates that the resistance to cereal cyst nematode in Taikong 6 is controlled by two major genes with additive-dominant effects plus polygene effect. The major gene heritability of resistance for the cross of Yumai 47 × Taikong 6 to H. avenaee Zhengzhou population in the conditions of inoculation and field tests was 73.54% and 86.90%, indicating the two major genes with the dominant role to resistance of Taikong 6 to H. avenaee Zhengzhou population.

Key words: Taikong 6, Cereal cyst nematode, Heterodera avenae Zhengzhou population, Resistance, Genetic analysis

[1]Nicol J M, Elekçio?lu ? H, Bolat N, Rivoal R. The global importance of the cereal cyst nematode (Heterodera spp.) on wheat and international approaches to its control. Commun Agric Appl Biol Sci, 2007, 72: 677–686



[2]Rivoal R, Nicol J M. Past research on the cereal cyst nematode complex and future needs. In: Riley I T, Nicol J M, Dababat A A, eds. Cereal Cyst Nematodes: Status, Research and Outlook. Ankara, Turkey: CIMMYT Press, 2009. pp 3–10



[3]Wang M-Z(王明祖), Peng D-L(彭德良), Wu X-Q (武学勤). Studies on a cyst nematode wheat disease. J Huazhong Agric Univ (华中农业大学学报), 1991, 10(4): 352–356 (in Chinese with English abstract)



[4]Chen P-S(陈品三), Wang M-Z(王明祖), Peng D-L(彭德良). A study of cereal cyst nematode (Heterodera avenae Wollenweber) in China. Acta Phytopathol Sin (植物病理学报), 1992, 22(4): 339–343 (in Chinese with English abstract)



[5]Peng D L, Nicol J M, Li H M, Hou S Y, Li H X, Chen S L, Ma P, Li H L, Riley I T. Current knowledge of cereal cyst nematode (Heterodera avenae) on wheat in China. In: Riley I T, Nicol J M, Dababat A A, eds. Cereal Cyst Nematodes: Status, Research and Outlook. Ankara, Turkey: CIMMYT Press, 2009. pp 29–34



[6]Riley I T, Hou S Y, Chen S L. Crop rotational and spatial determinants of variation in Heterodera avenae (cereal cyst nematode) population density at village scale in spring cereals at high altitude on the Tibetan Plateau, Qinghai, China. Aust Plant Pathol, 2010, 39: 424–430



[7]Li H-X(李惠霞), Liu Y-E(柳永娥), Wei Z(魏庄), Li M-Q(李敏全). The detection of Heterodera avenae from the cereal field in autonomous region of Tibet and Xinjiang. In: Liao J-L(廖金铃), Peng D-L(彭德良), Duan Y-X(段玉玺), Jian H(简恒), Li H-M(李红梅), eds. Nematology Research in China (中国线虫学研究), Vol. 4. Beijing: China Agricultural Science and Technology Press, 2012. pp 164–165 (in Chinese)



[8]Peng D-L(彭德良), Huang W-K(黄文坤), Sun J-H(孙建华), Liu C-J(刘崇俊), Zhang H-M(张辉民). First report of cereal cyst nematode (Heterodera avenae) in Tianjin, China. In: Liao J-L(廖金铃), Peng D-L(彭德良), Duan Y-X(段玉玺), Jian H(简恒), Li H-M(李红梅), eds. Nematology Research in China (中国线虫学研究), Vol. 4. Beijing: China Agricultural Science and Technology Press, 2012. pp 162–163 (in Chinese)



[9]Nicol J, Rivoal R, Taylor S, Zaharieva M. Global importance of cyst (Heterodera spp.) and lesion nematodes (Pratylenchus spp.) on cereals: distribution, yield loss, use of host resistance and integration of molecular tools. Nematol Monogr Perspect, 2003, 2: 1–19



[10]Zheng J-W(郑经武). Wheat cyst nematode disease and control in Australia. World Agric (世界农业), 1995, (3): 36–37 (in Chinese)



[11]Zheng J-W(郑经武), Lin M-S(林茂松), Chen H-R(程瑚瑞), Fang Z-D(方中达). Resistance of cereal cultivars to cereal cyst, Heterodera avenae. Acta Phytophyl Sin (植物保护学报), 1999, 26(3): 250–254 (in Chinese with English abstract)



[12]Wang Z-Y(王振跃), Gao S-F(高书峰), Li H-L(李洪连), Sha G-L(沙广乐), Yu M-Q(余懋群). Resistance of different wheat cultivars to cereal cyst. J Henan Agric Sci (河南农业科学), 2006, (5): 50–52 (in Chinese)



[13]Yuan H-X(袁虹霞), Li H-L(李洪连), Xing X-P(邢小萍), Sun J-W(孙君伟), Nian G-L(年高磊), Hou X-S(侯兴松). Resistance of wheat cultivars in Huanghuai area to Heterodera avenae Xingyang population. In: Peng Y-L(彭友良), Wang Z-H(王宗华), eds. Proceedings of the 2010 Annual Meeting of Chinese Society for Plant Pathology (中国植物病理学会2010年学术年会论文集). Beijing: China Agricultural Science and Technology Press, 2010. pp 552–555 (in Chinese)



[14]Li H J, Cui L, Li H L, Wang X M, Murray T D, Conner R L, Wang L J, Gao X, Sun Y, Sun S C, Tang W H. Effective resources of wheat and wheat-Thinopyrum derivatives for resistance to Heterodera filipjevi in China. Crop Sci, 2012, 53: 1209–1217



[15]Cui L(崔磊), Gao X(高秀), Wang X-M(王晓鸣), Jian H(简恒), Tang W-H(唐文华), Li H-L(李洪连), Li H-J(李洪杰). Characterization of interaction between wheat roots with different resistance and Heterodera filipjevi. Acta Agron Sin (作物学报), 2012, 38(6): 1009–1017 (in Chinese with English abstract)



[16]Ou S-Q(欧师琪), Peng D-L(彭德良), Li Y(李玉), Wang YJ(王永江). Restriction fragment length polymorphism and sequences analysis of rDNA-ITS region of cereal cyst nematode (Heterodera avenae) on wheat from Zhengzhou. Acta Phytopathol Sin (植物病理学报), 2008, 38(4): 407–413 (in Chinese with English abstract)



[17]Yuan H X, Sun J W, Yang W X, Xing X P, Wang Z Y, Riley I, Li H L. New pathotypes of Heterodera avenae (cereal cyst nematode) from winter wheat in Zhengzhou, Henan, China. Aust Plant Pathol, 2010, 39: 107–111



[18]Fang Z-D(方中达). Research Methods of Plant Disease (植病研究法). Beijing: Agriculture Press, 1998. pp 310–311



[19]Yuan H-X(袁虹霞), Zhang F-X(张福霞), Zhang J-J(张佳佳), Hou X-S(侯兴松), Li H-J(李洪杰), Li H-L(李洪连). Resistance of CIMMYT wheat germplasm to Heterodera filipjevi Xuchang population from Henan province, China. Acta Agron Sin (作物学报), 2011, 37(11): 1956−1966 (in Chinese with English abstract)



[20]Gai J-Y(盖钧镒), Zhang Y-M(章元明), Wang J-K(王建康). Genetic system of plant quantitative trait (植物数量性状遗传体系). Beijing: Science Press, 2003. pp 145–156 (in Chinese)



[21]Cotten J, Hayes J D. Genetic resistance to the cereal cyst nematode (Heterodera avenae). Heredity, 1969, 24: 593–600



[22]O’Brien P C, Sparrow D H B, Fisher J M. Inheritance of resistance to Heterodera avenae in barley. Nematologica, 1979, 25: 348–352



[23]Clamot T G, Rivoal R. Genetic resistance to cereal cyst nematode Heterodera Avenae Woll. in wild oat Avena sterilis I. 376. Euphytica, 1984, 33: 27–32
[1] 王好让, 张勇, 于春淼, 董全中, 李微微, 胡凯凤, 张明明, 薛红, 杨梦平, 宋继玲, 王磊, 杨兴勇, 邱丽娟. 大豆突变体ygl2黄绿叶基因的精细定位[J]. 作物学报, 2022, 48(4): 791-800.
[2] 刘磊, 詹为民, 丁武思, 刘通, 崔连花, 姜良良, 张艳培, 杨建平. 玉米矮化突变体gad39的遗传分析与分子鉴定[J]. 作物学报, 2022, 48(4): 886-895.
[3] 傅华英, 张婷, 彭文静, 段瑶瑶, 许哲昕, 林艺华, 高三基. 甘蔗新品种(系)苗期白条病人工接种抗性鉴定与评价[J]. 作物学报, 2021, 47(8): 1531-1539.
[4] 江建华, 张武汉, 党小景, 荣慧, 叶琴, 胡长敏, 张瑛, 何强, 王德正. 水稻核不育系柱头性状的主基因+多基因遗传分析[J]. 作物学报, 2021, 47(7): 1215-1227.
[5] 吴然然, 林云, 陈景斌, 薛晨晨, 袁星星, 闫强, 高营, 李灵慧, 张勤雪, 陈新. 绿豆雄性不育突变体msm2015-1的遗传学与细胞学分析[J]. 作物学报, 2021, 47(5): 860-868.
[6] 蒋成功, 石慧敏, 王红武, 李坤, 黄长玲, 刘志芳, 吴宇锦, 李树强, 胡小娇, 马庆. 玉米籽粒突变体smk7的表型分析和基因定位[J]. 作物学报, 2021, 47(2): 285-293.
[7] 张雪翠, 孙素丽, 卢为国, 李海朝, 贾岩岩, 段灿星, 朱振东. 河南大豆新品系抗大豆疫霉根腐病基因鉴定[J]. 作物学报, 2021, 47(2): 275-284.
[8] 张雪翠,钟超,段灿星,孙素丽,朱振东. 大豆品种郑97196抗疫霉病基因RpsZheng精细定位[J]. 作物学报, 2020, 46(7): 997-1005.
[9] 田士可, 秦心儿, 张文亮, 董雪, 代明球, 岳兵. 玉米雄性不育突变体mi-ms-3的遗传分析及分子鉴定[J]. 作物学报, 2020, 46(12): 1991-1996.
[10] 莫祎,孙志忠,丁佳,余东,孙学武,盛夏冰,谭炎宁,袁贵龙,袁定阳,段美娟. 水稻白条纹叶突变体wsl1的遗传分析及基因精细定位[J]. 作物学报, 2019, 45(7): 1050-1058.
[11] 崔月,陆建农,施玉珍,殷学贵,张启好. 蓖麻株高性状主基因+多基因遗传分析[J]. 作物学报, 2019, 45(7): 1111-1118.
[12] 王晓娟,潘振远,刘敏,刘忠祥,周玉乾,何海军,邱法展. 一个新的玉米silky1基因等位突变体的遗传分析与分子鉴定[J]. 作物学报, 2019, 45(11): 1649-1655.
[13] 李英双,胡丹,聂蛟,黄科慧,张玉珂,张园莉,佘恒志,方小梅,阮仁武,易泽林. 甜荞株高和茎粗的遗传分析[J]. 作物学报, 2018, 44(8): 1185-1195.
[14] 肖明纲, 宋凤景, 孙兵, 左辛, 赵广山, 辛爱华, 李柱刚. 玉米大斑病广谱抗性外引自交系的发掘与抗病基因初步鉴定[J]. 作物学报, 2018, 44(04): 614-619.
[15] 李竹, 许莉萍, 苏亚春, 吴期滨, 成伟, 孙婷婷, 高世武. 基于田间表型和Bru1基因检测分析甘蔗褐锈病抗性遗传[J]. 作物学报, 2018, 44(02): 306-312.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2