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作物学报 ›› 2013, Vol. 39 ›› Issue (04): 649-656.doi: 10.3724/SP.J.1006.2013.00649

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蔗细茎野生种核心种质构建

齐永文,樊丽娜,罗青文,王勤南,陈勇生,黄忠兴,刘睿,刘少谋,邓海华*,李奇伟*   

  1. 广州甘蔗糖业研究所 / 广东省甘蔗改良与生物炼制重点实验室, 广东广州 510316
  • 收稿日期:2012-09-18 修回日期:2012-12-12 出版日期:2013-04-12 网络出版日期:2013-01-28
  • 通讯作者: 李奇伟, E-mail: liqiwei66@163.com, Tel: 020-84168478; 邓海华, E-mail: haihuadeng@126.com, Tel: 020-84178327
  • 基金资助:

    本研究由国家自然科学基金项目(30800700), 国家现代农业产业技术体系建设专项(CARS-20-1-4)和广东省科技计划项目(2011B060400019)资助。

Establishment of Saccharum spontaneum L. Core Collections

QI Yong-Wen,FAN Li-Na,LUO Qing-Wen,WANG Qin-Nan,CHEN Yong-Sheng,HUANG Zhong-Xing,LIU Rui,LIU Shao-Mou,DENG Hai-Hua*,LI Qi-Wei*   

  1. Guangdong Key Laboratory of Sugarcane Improvement and Biorefine, Guangzhou Sugarcane Industry Research Institute, Guangzhou 510316, China
  • Received:2012-09-18 Revised:2012-12-12 Published:2013-04-12 Published online:2013-01-28
  • Contact: 李奇伟, E-mail: liqiwei66@163.com, Tel: 020-84168478; 邓海华, E-mail: haihuadeng@126.com, Tel: 020-84178327

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被引次数

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摘要：

以来自我国10个省(市)、自治区的540份甘蔗细茎野生种无性系为材料，根据采集地信息及其在20对SSR引物上的分子标记数据和15个表型性状资料，开展核心种质构建研究。不同取样量(5%、10%、20%、30%、40%、50%、60%、70%、80%和90%)分析表明，10%的取样比例可获得70%以上的变异保留比例，是较好的核心种质取样规模；对5种采集地分组取样策略(等量法、简单比例法、平方根比例法、对数比例法和多样性比例法)和2种无分组取样策略(最大变异保留法和随机抽样法)比较表明，简单比例法获得的核心种质代表性最好，为最优取样策略。最后，在简单比例法取样筛选出的54份核心样品中，又通过定向选择补充了6份具有优异表型性状的材料，构建了含60份无性系的甘蔗细茎野生种核心种质，分子和表型检验都表明本研究所构建的核心种质具有较好的代表性和遗传多样性。

关键词: 甘蔗细茎野生种, 核心种质, SSR, 表型性状

Abstract:

Five hundred and forty accessions of Saccharum spontaneum L. from ten provinces of China were characterized by geographical information, molecular markers on 20 SSRs and 15 agro-morphological traits to establish a core collection. Ten different sampling ratios of accessions were tested, including 5% and 10% to 90% with an interval of 10%. The results showed that 10% of samples could conserve more than 70% of variations, which indicated that 10% was an adequate sampling ratio for establishing the core collection. A comparison of the efficiencies of five sampling strategies by geographical grouping (constant, proportional, square root, logarithmic, and genetic diversity-depend) and two non-grouping sampling strategies (maximization and random sampling), indicated proportional strategy was optimal in term of establishing the most representative core collection. Thus, a core collection of 60 accessions, including 54 selected based on proportional strategy and six accessions selected by their specific agro-morphological traits, was constructed. The tests on SSR data and agro-morphological characters demonstrated that the core collection have high genetic diversity and a good representative to the entire collection.

Key words: Saccharum spontaneum L., Core collection, SSR, Agro-morphological traits

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