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作物学报 ›› 2015, Vol. 41 ›› Issue (07): 1127-1135.doi: 10.3724/SP.J.1006.2015.01127

• 研究简报 • 上一篇    下一篇

水分胁迫下甘蔗差异表达基因筛选及激素相关基因分析

李长宁,谢金兰,王维赞,梁强,李毅杰,董文斌,刘晓燕,杨丽涛*,李杨瑞*   

  1. 中国农业科学院甘蔗研究中心 / 广西农业科学院甘蔗研究所 / 农业部广西甘蔗生物技术与遗传改良重点实验室 / 广西甘蔗遗传改良重点实验室,广西南宁530007
  • 收稿日期:2014-12-08 修回日期:2015-05-04 出版日期:2015-07-12 网络出版日期:2015-05-15
  • 基金资助:

    本研究由国家高技术研究计划(863计划)项目 (2013AA102604), 科技部国际合作项目(2013DFA31600), 广西农科院团队项目(桂农科2011YT01), 广西八桂学者、特聘专家专项经费, 广西自然科学基金项目(2014GXNSFBA118085)和广西农科院基本科研业务专项(桂农科2014YD01)资助。

Screening of Differentially Expressed Genes and Analysis of Plant Hormones Related Genes under Water Stress in Sugarcane

LI Chang-Ning,XIE Jin-Lan,WANG Wei-Zan,LIANG Qiang,LI Yi-Jie,DONG Wen-Bin,LIU Xiao-Yan,YANG Li-Tao*,LI Yang-Rui*   

  1. Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences / Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture / Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007, China
  • Received:2014-12-08 Revised:2015-05-04 Published:2015-07-12 Published online:2015-05-15

摘要:

甘蔗是经济和环境上日益重要的C4作物,干旱在全球范围内显著限制甘蔗产量。了解甘蔗对水分胁迫反应的分子机制将有助于甘蔗抗旱性的分子遗传改良。利用基因芯片技术分析水分胁迫下甘蔗叶片的15 593个基因的表达谱,结果表明,中、重度胁迫下的差异表达基因数量分别为300个和853个,中度胁迫中差异基因以上调表达为主,重度胁迫中下调表达占多数。注释结果显示,差异表达基因分子功能主要为结合、载体和催化活性,主要参与代谢、细胞和生物调控等生物过程。此外,功能未明确的假定蛋白和无匹配信息的基因序列仍占据注释结果的相当一部分,表明还有大量的基因尚待发掘。在水分胁迫下,甘蔗内源ABA和IAA含量显著上升而GA含量显著受到抑制。以参与生物进程分类,对植物激素相关基因进行筛选并分析,发现激素响应表达基因代谢途径具有多样性,显示了激素代谢网络的交叉性与复杂性。挑选9个差异表达程度不同的基因进行实时荧光定量PCR检测,表明芯片数据具有良好的重复性。

关键词: 甘蔗, 水分胁迫, 基因芯片, 植物激素, 基因表达谱

Abstract:

Sugarcane is an increasingly economically and environmentally important C4 crop. Water stress limits enormously sugarcane productivity worldwide, and understanding the molecular mechanisms for sugarcane stress responses will be useful for sugarcane improvement by genetic manipulation. To investigate the transcriptome changes in response to water stress, we used microarrays to profile expressions of 15 593 genes in sugarcane exposed to drought. The results indicated that 300 and 853 differentially expressed genes were detected under moderate and severe water stresses, respectively. The expression of differentially expressed genes treated with moderate water stress was mainly up-regulated, however that treated with severe water stress was mainly down-regulated. To further characterize these genes, we used Gene Ontology (GO) for their annotation, the results showed that differentially expressed genes possessed the functions of binding, transporter, molecular transducer and catalytic activities and were involved in metabolic, biological regulation and cellular processes. Besides, hypothetical protein and no match annotated results were found to fill a large part of those genes, indicating that effective approach should be adopted to discover novel genes in sugarcane genomics. Water stress resulted in an increase in ABA and IAA contents but a depression in GA content. Classified by biological process, 46 plant hormone related genes were selected, further annotation analysis showed that the metabolic pathways of some plant hormone responsive genes were diverse or had crosstalk with each other, indicating the intersectionality and complexity of plant hormone signaling pathway. Additionally, the relative expressions of nine selected genes were validated by quantitative real time PCR (qRT-PCR), further confirming the reliability of microarray results.

Key words: Sugarcane, Water stress, Microarray, Plant hormone, Gene expression profiles

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