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作物学报 ›› 2017, Vol. 43 ›› Issue (09): 1308-1318.doi: 10.3724/SP.J.1006.2017.01308

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

不同脱水剂对杂交水稻制种种子质量及基因表达的影响

沈杭琪1,胡伟民1,林程1,关亚静1,刘宏友2,安建宇1,胡晋1,*   

  1. 1浙江大学农业与生物技术学院种子科学中心, 浙江杭州310058;2浙江省浦江县种子管理站, 浙江金华322200
  • 收稿日期:2016-12-09 修回日期:2017-05-10 出版日期:2017-09-12 网络出版日期:2017-05-25
  • 通讯作者: 胡晋, E-mail: jhu@zju.edu.cn
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项(201203052), 浙江省科技厅项目(2013C02005, 2013C32023), 浙江省自然科学基金项目(LZ14C130002, LY15C130002)和江苏省现代作物生产协同创新中心项目资助。

Effects of Different Dehydrating Agents on Seed Quality and Gene Expression inHybrid Rice Seed Production

SHEN Hang-Qi1,HU Wei-Min1,LIN Cheng1,GUAN Ya-Jing1,LIU Hong-You2,AN Jian-Yu1,HU Jin1,*   

  1. 1 Seed Science Center, Institute of Crops Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; 2 Seed Management Station of Pujiang County, Jinhua 322200, China
  • Received:2016-12-09 Revised:2017-05-10 Published:2017-09-12 Published online:2017-05-25
  • Contact: Hu JIn, E-mail: jhu@zju.edu.cn
  • Supported by:

    This study was supported by the China Special Fund for Agro-Scientific Research in the Public Interest (201203052), the Project of Science and Technology Department of Zhejiang Province (2013C02005, 2013C32023), the Natural Sciences Fund of Zhejiang Province (LZ14C130002, LY15C130002), and Jiangsu Collaborative Innovation Center for Modern Crop Production.

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摘要：

以Y两优689杂交水稻品种为材料，研究了不同脱水剂对其制种种子脱水及种子质量的影响。结果表明，11种脱水剂均能加快种子成熟后期脱水，7号和9号脱水剂喷施后5 d，种子水分分别下降4.6%和3.6%，与对照相比，两种处理对种子千粒重、发芽和幼苗生长无不良影响，种子可溶性蛋白、可溶性糖、ABA和GA3含量则显著高于对照。种子室温贮藏6个月后，与对照相比，7号、8号和9号脱水剂处理对种子发芽和幼苗生长无显著影响，7号和9号脱水剂处理的种子可溶性蛋白含量、α-淀粉酶活性、ABA含量与对照无显著差异。7号比9号脱水效果好，可作为Y两优689的脱水剂。进一步研究发现，种子贮藏前，7号、8号和9号脱水剂处理能提高种子中OsNECD和OsNCED2基因的表达，降低OsGA2ox1的表达。与未贮藏种子相比，3种脱水剂处理的种子OsNECD1和OsNCED2表达量下降，OsCYP707A5和OsGA2ox1表达量上升。表明经过6个月贮藏，脱水剂处理对种子ABA合成相关基因和GA3分解相关基因表达的影响减弱，同时OsCYP707A5的高表达导致种子ABA含量减少，可能是种子贮藏后萌发力提高的主要原因。

关键词: 杂交水稻, 脱水剂, 种子质量, 成熟脱水, 基因表达

Abstract:

In order to accelerate seed dehydration process in hybrid rice seed production, the effects of different dehydrating agents on seed dehydration and quality of cv. Y Liangyou 689 were studied. Different dehydrating agents were sprayed on rice spike at late dough stage inseed production in the field. All the eleven combination treatments could accelerate seed dehydration. Among the treatments, No.7 and No.9 dehydrating agents significantly decreased seed moisture content by 4.6% and 3.6%, respectively as compared with the control, and had no negative effects on 1000-grain weight, seed germination and seedling growth. Moreover, these two agents significantly increased the contents of soluble protein, soluble sugar, ABA and GA3 in seeds. After storage at room temperature for sixmonths, No.7, No.8, and No.9 agents had no negative effects on seed germination and seedling growth as compared with the control. And there were no significant differences in soluble protein content, α-amylase activity and ABA content among No.7, No.9 agents and the control. In addition, No.7 agent treatment had better effect in decreasing seed moisture content than No.9 agent, suggestingthat No.7 agent was a suitable seed dehydrating agent inhybrid rice (cv. Y Liangyou 689) seed production. Further studies showed that No.7, No.8, and No.9 agents could increase the expression of OsNECD1, OsNCED2 and reduce the expression of OsGA2ox1 in the seeds. As compared with the seeds without storage, after the storage for six month the expression of OsNECD1 and OsNCED2 decreased, however the expression of OsCYP707A5 and OsGA2ox1 increased in seeds treated by the three kinds of dehydrating agents. The results showed the effect of dehydrating agents on ABA biosynthesis genes and GA3 catabolism genes in seedswas weakenedby storage, and the high expression of OsCYP707A5 resulted in the decline of ABA content in seeds, which might be the main reason for increasing seed germination.

Key words: Hybrid rice, Dehydration agent, Seed quality, Mature dehydration, Gene expression

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