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作物学报 ›› 2017, Vol. 43 ›› Issue (09): 1308-1318.doi: 10.3724/SP.J.1006.2017.01308

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

不同脱水剂对杂交水稻制种种子质量及基因表达的影响

沈杭琪1,胡伟民1,林程1,关亚静1,刘宏友2,安建宇1,胡晋1,*   

  1. 1浙江大学农业与生物技术学院种子科学中心, 浙江杭州310058;2浙江省浦江县种子管理站, 浙江金华322200
  • 收稿日期:2016-12-09 修回日期:2017-05-10 出版日期:2017-09-12 网络出版日期:2017-05-25
  • 通讯作者: 胡晋, E-mail: jhu@zju.edu.cn
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项(201203052), 浙江省科技厅项目(2013C02005, 2013C32023), 浙江省自然科学基金项目(LZ14C130002, LY15C130002)和江苏省现代作物生产协同创新中心项目资助。

Effects of Different Dehydrating Agents on Seed Quality and Gene Expression inHybrid Rice Seed Production

SHEN Hang-Qi1,HU Wei-Min1,LIN Cheng1,GUAN Ya-Jing1,LIU Hong-You2,AN Jian-Yu1,HU Jin1,*   

  1. 1 Seed Science Center, Institute of Crops Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; 2 Seed Management Station of Pujiang County, Jinhua 322200, China
  • Received:2016-12-09 Revised:2017-05-10 Published:2017-09-12 Published online:2017-05-25
  • Contact: Hu JIn, E-mail: jhu@zju.edu.cn
  • Supported by:

    This study was supported by the China Special Fund for Agro-Scientific Research in the Public Interest (201203052), the Project of Science and Technology Department of Zhejiang Province (2013C02005, 2013C32023), the Natural Sciences Fund of Zhejiang Province (LZ14C130002, LY15C130002), and Jiangsu Collaborative Innovation Center for Modern Crop Production.

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摘要：

以Y两优689杂交水稻品种为材料，研究了不同脱水剂对其制种种子脱水及种子质量的影响。结果表明，11种脱水剂均能加快种子成熟后期脱水，7号和9号脱水剂喷施后5 d，种子水分分别下降4.6%和3.6%，与对照相比，两种处理对种子千粒重、发芽和幼苗生长无不良影响，种子可溶性蛋白、可溶性糖、ABA和GA3含量则显著高于对照。种子室温贮藏6个月后，与对照相比，7号、8号和9号脱水剂处理对种子发芽和幼苗生长无显著影响，7号和9号脱水剂处理的种子可溶性蛋白含量、α-淀粉酶活性、ABA含量与对照无显著差异。7号比9号脱水效果好，可作为Y两优689的脱水剂。进一步研究发现，种子贮藏前，7号、8号和9号脱水剂处理能提高种子中OsNECD和OsNCED2基因的表达，降低OsGA2ox1的表达。与未贮藏种子相比，3种脱水剂处理的种子OsNECD1和OsNCED2表达量下降，OsCYP707A5和OsGA2ox1表达量上升。表明经过6个月贮藏，脱水剂处理对种子ABA合成相关基因和GA3分解相关基因表达的影响减弱，同时OsCYP707A5的高表达导致种子ABA含量减少，可能是种子贮藏后萌发力提高的主要原因。

关键词: 杂交水稻, 脱水剂, 种子质量, 成熟脱水, 基因表达

Abstract:

In order to accelerate seed dehydration process in hybrid rice seed production, the effects of different dehydrating agents on seed dehydration and quality of cv. Y Liangyou 689 were studied. Different dehydrating agents were sprayed on rice spike at late dough stage inseed production in the field. All the eleven combination treatments could accelerate seed dehydration. Among the treatments, No.7 and No.9 dehydrating agents significantly decreased seed moisture content by 4.6% and 3.6%, respectively as compared with the control, and had no negative effects on 1000-grain weight, seed germination and seedling growth. Moreover, these two agents significantly increased the contents of soluble protein, soluble sugar, ABA and GA3 in seeds. After storage at room temperature for sixmonths, No.7, No.8, and No.9 agents had no negative effects on seed germination and seedling growth as compared with the control. And there were no significant differences in soluble protein content, α-amylase activity and ABA content among No.7, No.9 agents and the control. In addition, No.7 agent treatment had better effect in decreasing seed moisture content than No.9 agent, suggestingthat No.7 agent was a suitable seed dehydrating agent inhybrid rice (cv. Y Liangyou 689) seed production. Further studies showed that No.7, No.8, and No.9 agents could increase the expression of OsNECD1, OsNCED2 and reduce the expression of OsGA2ox1 in the seeds. As compared with the seeds without storage, after the storage for six month the expression of OsNECD1 and OsNCED2 decreased, however the expression of OsCYP707A5 and OsGA2ox1 increased in seeds treated by the three kinds of dehydrating agents. The results showed the effect of dehydrating agents on ABA biosynthesis genes and GA3 catabolism genes in seedswas weakenedby storage, and the high expression of OsCYP707A5 resulted in the decline of ABA content in seeds, which might be the main reason for increasing seed germination.

Key words: Hybrid rice, Dehydration agent, Seed quality, Mature dehydration, Gene expression

[1]周可金, 官春云, 肖文娜, 谭太龙. 催熟剂对油菜角果光合特性、品质及产量的影响. 作物学报, 2009, 35: 1369–1373
Zhou K J, Guan C Y, Xiao W N, Tan T L. Effects of chemical ripeners on photosynthetic characteristics of pods and rapeseed quality and yield. Acta Agron Sin, 2009, 35: 1369–1373 (in Chinese with English abstract)
[2]陈轶, 张倩倩. 催枯剂敌草快在单季晚稻上的应用效果初探. 中国稻米, 2011, 17(2): 45–46
Chen Y, Zhang Q Q. Effect of diquat on single-crop rice.China Rice, 2011, 17(2): 45–46 (in Chinese)
[3]郝洪波, 崔海英, 李明哲. 不同化学脱水剂对谷子机械收获产量的影响. 河北农业科学, 2013, 17(1): 6–8
Hao H B, Cui H Y, Li M Z. Effect of different chemical drying agents on the mechanical harvesting yield in foxtail millet.J Hebei Agric Sci, 2013, 17(1): 6–8 (in Chinese with English abstract)
[4]胡伟民, 王齐天, 曹栋栋, 阮关海, 朱丽伟, 关亚静, 谢潜程, 胡晋. 不同脱水剂对杂交水稻制种种子成熟脱水和质量的影响. 浙江农业学报, 2015, 27: 901–906
Hu W M, Wang Q T, Cao D D, Ruan G H, Zhu L W, Guan Y J, Xie Q C, Hu J. Effects of different dehydrating agents on seed dehydration and quality during seed maturation of hybrid rice seed production. Acta Agric Zhejiangensis, 2015, 27: 901–906 (in Chinese with English abstract)
[5]朱丽伟, 曹栋栋, 付玉营, 胡琦娟, 利站, 关亚静, 胡晋. 可溶性寡糖和小分子的热激蛋白与杂交水稻种子成熟过程中发芽能力及种子活力相关. 作物学报, 2016, 42: 714–724
Zhu LW, Cao D D, Fu Y Y, Hu Q J, Li Z, Guan Y J, Hu J. Soluble oligosaccharide and small heat shock protein correlated with seed germination and vigor during hybrid rice seed maturation. Acta Agron Sin, 2016, 42: 714–724 (in Chinese with English abstract)
[6]刘海英, 王华华, 崔长海, 王曼, 郭净净, 文昭普, 李安琪. 可溶性糖含量测定(蒽酮法)实验的改进. 实验室科学, 2013, 16(2): 19–20
Liu H Y, Wang H H, Cui C H, Wang M, Guo J J, Wen Z P, Li A Q. Experiment improvement of the soluble sugar content determination by enthrone colorimetric method. Lab Sci, 2013, 16(2): 19–20 (in Chinese with English abstract)
[7]王孝平, 邢树礼. 考马斯亮蓝法测定蛋白含量的研究. 天津化工, 2009, 23(3): 40–42
Wang X P, Xing S L. Determination of protein using the method of coomassie brilliant blue.Tianjin Chem Ind, 2009, 23(3): 40–42 (in Chinese)
[8]李雯, 邵远志, 陈维信. 淀粉酶活性测定方法的改进. 植物生理学通讯, 2005, 41: 655–656
Li W, Shao Z Y, Chen W X. Improved method for determining amylase activity. Plant Physiol Commun, 2005, 41: 655–656 (in Chinese with English abstract)
[9]秦国臣. 内源激素ABA含量、抗氧化物酶活性和种子质量在丝瓜种子发育过程中的变化. 浙江大学硕士学位论文, 浙江杭州, 2013
Qin G C. Changes of Endogens Hormone ABA Content, Antioxidant Activities and Seed Quality during seed Development in Sponge Gourd. MS Thesis of Zhejiang University, Hangzhou, China, 2013 (in Chinese with English abstract)
[10]辛慧慧, 李防洲, 侯振安, 冶军, 康文晶, 罗建. 低温胁迫下棉花幼苗对外源水杨酸的生理响应. 植物生理学报, 2014, 50: 660–664
Xin H H, Li F Z, Hou Z A, Ye J, Kang W J, Luo J. Physiological response of cotton (Gossypium hirsutum L.) seedling to exogenous salicylic acid under low temperature stress. Plant Physiol J, 2014, 50: 660–664 (in Chinese with English abstract)
[11]李煦, 汪晓峰. 油菜(Brassica napus L.)种子脱水耐性获得过程中肌醇半乳糖苷合成酶活性与可溶性糖含量的变化. 植物生理学报, 2011, 47: 1173–1180
Li X, Wang X F. Galactinol synthase activity and soluble carbohydrate contents during acquisition of seed desiccation tolerance in Brassica napus L. Plant Physiol J, 2011, 47: 1173–1180 (in Chinese with English abstract)
[12]徐亮, 包维楷,何永华. 种子贮藏物质变化及其贮藏生理. 种子, 2003, (5): 60–63
Xu L, Bao W K, He Y H. Changes of seed substance and physiology during storage. Seed, 2003, (5): 60–63 (in Chinese)
[13]何俊瑜, 任艳芳, 朱诚, 蒋德安. 镉胁迫对不同水稻品种种子萌发、幼苗生长和淀粉酶活性的影响. 中国水稻科学, 2008, 22: 399–404
He J Y, Ren Y F, Zhu C, Jiang D A. Effects of cadmium stress on seed germination, seedling growth and amylase activities in rice. Chin J Rice Sci, 2008, 22: 399–404 (in Chinese with English abstract)
[14]张桂莲, 杨定照, 张顺堂, 陈立云. 不同成熟度对水稻种子萌发及其生理特性的影响.植物生理学报, 2012, 48: 272–276
Zhang G L, Yang D Z, Zhang S T, Chen L Y. Effects of different maturity on germination and physiological characteristics of rice (Oryza sativa L.) seeds. Plant Physiol J, 2012, 48: 272–276 (in Chinese with English abstract)
[15]Liu F, Zhang H, Wu G, Sun J, Hao L, Ge X, Yu J, Wang W. Sequence variation and expression analysis of seed dormancy- and germination-associated ABA- and GA- related genes in rice cultivars. Front Plant Sci, 2011, 2: 17
[16]Perales L, Arbona V, Gómez-Cadenas A, Cornejo M J, Sanz A. A relationship between tolerance to dehydration of rice cell lines and ability for ABA synthesis under stress. Plant Physiol Biochem, 2005, 43: 786–792
[17]胡晋. 种子学. 北京: 中国农业出版社, 2014. pp 25–26
Hu J. Seed Science. Beijing: China Agriculture Press, 2014. pp 25–26 (in Chinese)
[18]周可金, 吴奇志, 肖文娜, 张振乾, 官春云. 化学催熟剂对油菜角果内源激素含量的影响. 南京农业大学学报, 2011, 34(6): 13–19
Zhou K J, Wu Q Z, Xiao W N, Zhang Z Q, Guan C Y. Effects of ripener on the endogenous hormones of pods during rapeseed maturity. J Nanjing Agric Univ, 2011, 34(6): 13–19 (in Chinese with English abstract)
[19]Shu K, Liu X D, Xie Q, He Z. Two faces of one seed: hormonal regulation of dormancy and germination. Mol Plant, 2016, 9: 45–70
[20]武明珠, 徐晟, 崔为体, 张博, 刘雅辉, 凌腾芳. 血红素加氧酶介导GA对小麦糊粉层中α-淀粉酶的诱导表达. 植物生理学报, 2009, 45: 483–486
Wu M Z, Xu S, Cui W T, Zhang B, Liu Y H, Ling T F. Involvement of heme oxygenase in gibberellic acid-induced a-amylase expression in wheat aleurone layer. Plant Physiol J, 2009, 45: 483–486 (in Chinese with English abstract)
[21]曹雅君, 江玲, 罗林广, 翟虎渠, 志村英二, 杨世湖, 万建民. 水稻品种休眠特性的研究. 南京农业大学学报, 2001, 24(2): 1–5
Cao Y J, Jiang L, Luo L G, Zhai H Q, Shimura E, Yang S H, Wan J M. A study on seed dormancy in rice (Oryza sativa L.).J Nanjing Agric Univ, 2001, 24(2): 1–5 (in Chinese with English abstract)
[22]Du W, Cheng J, Cheng Y, Wang L, He Y, Wang Z, Zhang H. Physiological characteristics and related gene expression of after-ripening on seed dormancy release in rice. Plant Biol, 2015, 17: 1156–1164
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