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作物学报 ›› 2018, Vol. 44 ›› Issue (01): 15-23.doi: 10.3724/SP.J.1006.2018.00015

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

控制普通野生稻种子休眠性QTL的定位

孙爱伶1，伍洪铭1，陈高明1，张天雨1，曹鹏辉1，刘世家1，江玲1,*，万建民1,2   

  1. 1南京农业大学作物遗传与种质创新国家重点实验室 / 农业部长江中下游粳稻生物学与遗传育种重点实验室 / 江苏省植物基因工程技术研究中心，江苏南京 210095；2中国农业科学院作物科学研究所 / 作物基因资源与遗传改良国家重大科学工程，北京100081
  • 收稿日期:2017-04-06 修回日期:2017-09-10 出版日期:2018-01-12 网络出版日期:2017-10-31
  • 基金资助:

    本研究由国家重点研发计划项目(2016YFD0100101-08), 江苏省农业科技自主创新资金课题(CX[16]1029), 安徽省科技重大专项(16030701068)和江苏省现代作物生产协同创新中心项目资助。

Mapping of QTLs for Seed Dormancy in Oryza Rufipogon Griff.

SUN Ai-Ling1,WU Hong-Ming1,CHEN Gao-Ming1,ZHANG Tian-Yu1,CAO Peng-Hui1,LIU Shi-Jia1,JIANG Ling1,*,WAN Jian-Min1,2   

  1. 1State Key Loboratory of Crop Genetics and Germplasm Enhancement / Key Laboratory of Biology, Genetics and Breeding of Japonica Rice in Mid-lower Yangtze River, Ministry of Agriculture / Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China; 2National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2017-04-06 Revised:2017-09-10 Published:2018-01-12 Published online:2017-10-31
  • Supported by:

    This study was supported by the National Key Research and Development Program of China (2016YFD0100101-08), the Agricultural Science and Technology Independent Innovation Fund Project of Jiangsu Province (CX[16]1029), Science and Technology Major Project of Anhui Province (16030701068), and Jiangsu Collaborative Innovation Center for Modern Crop Production.

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摘要：

水稻种子休眠性是关系到稻米品质和稻种质量的一个重要农艺性状。研究水稻种子休眠性遗传及分子机制对培育具有适度休眠性的优良水稻品种具有重要意义。本研究以籼稻品种9311为受体、普通野生稻为供体的染色体片段置换系群体为材料，在后熟不同时间检测群体种子休眠性，对控制种子休眠性的QTL进行定位分析，共定位到14个QTL，分布在第3、第4、第5、第6、第7、第10、第11、第12染色体上。筛选休眠性显著强于背景亲本9311的家系，分析这些家系携带的QTL数目，表明携带的位点越多，休眠性越强。进一步利用家系Q14与9311的F2群体验证了第7染色体标记RM180和RM21323之间存在一个效应较大的QTL qSD-7-2，该位点LOD值为18.49，可解释的表型变异率为33.53%，表明该位点是一个控制普通野生稻种子休眠性的主效QTL，且能稳定遗传。本研究为野生稻种子休眠基因的精细定位及克隆奠定了基础，且为培育强休眠性籼稻品种提供了育种材料。

关键词: 野生稻, 种子休眠, 染色体片段置换系, QTL

Abstract:

Seed dormancy of rice is an important agronomic trait related to rice quality and quantity. Studies on genetics and molecular mechanisms of rice seed dormancy are of great significance in breeding fine rice varieties with moderate dormancy. In this research, a set of chromosome segment substitution lines (CSSLs), derived from an indica rice variety 9311 as the recurrent parent and the Oryza rufipogon Griff. as the donor parent, were used to detect the QTLs for dormancy of seeds at different storage dates after harvest. A total of 14 QTLs were detected on chromosomes 3, 4, 5, 6, 7, 10, 11, and 12. The lines with significantly stronger dormancy than the background parent 9311 were selected, showing the more dormancy loci in the lines the more strong dormancy. The F2 population of the cross between Q14 and 9311 was used to verify the QTLs for seed dormancy. A significant dormancy locus qSD-7-2 was mapped on chromosome 7 between the markers RM180 and RM21323, its LOD was 18.49 and the phenotypic variation rate was 33.53%. On this major stable inherited QTL, the allele gene from Oryza rufipogon Griff. significantly increased the dormancy of seeds. These results are available for map-based cloning of major QTLs for seed dormancy, and provide the breeding materials for cultivating appropriate dormant rice varieties.

Key words: Oryza rufipogon Griff., seed dormancy, CSSL, QTL

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