中国黄麻炭疽病病原菌的分离鉴定及系统发育分析

doi:10.3724/SP.J.1006.2022.14035

作物学报 ›› 2022, Vol. 48 ›› Issue (3): 770-777.doi: 10.3724/SP.J.1006.2022.14035

• 研究简报 • 上一篇

中国黄麻炭疽病病原菌的分离鉴定及系统发育分析

郭艳春¹^,²^,^3**(), 姚嘉瑜¹^,²^,^3**(), 张镕斌¹^,², 陈思远¹^,²^,³, 何青垚¹^,²^,³, 陶爱芬¹^,², 方平平¹^,², 祁建民¹^,², 张列梅¹, 张立武¹^,²^,³^,^*()

¹福建农林大学农学院/作物遗传育种与综合利用教育部重点实验室/福建省作物设计育种重点实验室, 福建福州 350002
²福建农林大学农业农村部东南黄红麻实验观测站/福建省麻类种质资源共享平台/福建省南方经济作物遗传育种与多用途开发国际科技合作基地, 福建福州 350002
³福建农林大学海峡联合研究院基因组与生物技术中心, 福建福州 350002

收稿日期:2021-02-26 接受日期:2021-07-12 出版日期:2022-03-12 网络出版日期:2021-08-05
通讯作者: 张立武
作者简介:郭艳春, E-mail: 565358452@qq.com
姚嘉瑜, E-mail: 3533551417@qq.com第一联系人：^**同等贡献
基金资助:
国家自然科学基金项目(31771369);国家现代农业产业技术体系建设专项(CARS-19-E06)

Identification and phylogenetic analysis of the pathogen of jute anthracnose in China

GUO Yan-Chun¹^,²^,^3**(), YAO Jia-Yu¹^,²^,^3**(), ZHANG Rong-Bin¹^,², CHEN Si-Yuan¹^,²^,³, HE Qing-Yao¹^,²^,³, TAO Ai-Fen¹^,², FANG Ping-Ping¹^,², QI Jian-Min¹^,², ZHANG Lie-Mei¹, ZHANG Li-Wu¹^,²^,³^,^*()

¹Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops/Fujian Key Laboratory for Crop Breeding by Design/College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
²Experiment Station of Ministry of Agriculture and Rural Affairs for Jute and Kenaf in Southeast China/Public Platform of Fujian for Germplasm Resources of Bast Fiber Crops/Fujian International Science and Technology Cooperation Base for Genetics, Breeding and Multiple Utilization Development of Southern Economic Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
³Center for Genomics and Biotechnology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.

Received:2021-02-26 Accepted:2021-07-12 Published:2022-03-12 Published online:2021-08-05
Contact: ZHANG Li-Wu
About author:First author contact:^**Contributed equally to this work
Supported by:
National Natural Science Foundation of China(31771369);China Agriculture Research System(CARS-19-E06)

摘要/Abstract

摘要：

炭疽病是严重影响黄麻纤维产量和品质的主要真菌性病害, 明确炭疽病病原菌种类并测定其致病力有助于黄麻抗病育种和优异抗病基因资源挖掘。本研究从福建省福州市、福建省漳州市、河南省信阳市、安徽省六安市、浙江省杭州市、广西省南宁市和湖南省长沙市等7个黄麻生产区采集黄麻炭疽病病原菌样本, 分离纯化出92个菌株; rDNA-ITS区域的序列分析表明, 其中11个为典型炭疽病病原菌。LSU区域的系统进化树和形态学特征鉴定显示, ZZ4、GX19等10个菌株为胶胞炭疽菌(Colletotrichum gloeosporioides), 菌株CS3为黑线炭疽菌(Colletotrichum dematium)。人工接种的致病力测定表明, 不同炭疽菌菌株致病力存在显著差异, 其中胶胞炭疽菌菌株GX19致病力最强, 表现为优势菌株。这些结果为黄麻炭疽病抗性基因位点挖掘和有效防治奠定了基础。

关键词: 黄麻, 炭疽菌, 致病力测定, 系统发育

Abstract:

Anthracnose is a major fungal disease that seriously affects the yield and quality in jute fiber. Identifying the types of anthracnose pathogens and determining their pathogenicity will help jute disease-resistant breeding and develop elite disease-resistant gene resources. The 92 strains were isolated and purified from samples of diseased jute, which were collected from 7 jute production areas including Fuzhou and Zhangzhou in Fujian Province, Xinyang City in Henan Province, Liu’an City in Anhui Province, Hangzhou City in Zhejiang Province, Nanning City in Guangxi Province, and Changsha City in Hunan Province. The sequence analysis of the rDNA-ITS region showed that 11 out of the 92 strains were typical anthracnose pathogens. Further identification of the phylogenetic tree of the LSU region and morphological characteristics revealed that 10 strains, including ZZ4 and GX19, were Colletotrichum gloeosporioides, while the strain of CS3 was Colletotrichum dematium. The pathogenicity test of artificial inoculation indicated that there was a significant difference in pathogenicity between Colletotrichum gloeosporioides and Colletotrichum dematium. These results laid a basis for identification of resistance gene loci and effective controlling of anthracnose in jute.

Key words: jute, Colletotrichum, pathogenicity determination, phylogenetic tree

郭艳春, 姚嘉瑜, 张镕斌, 陈思远, 何青垚, 陶爱芬, 方平平, 祁建民, 张列梅, 张立武. 中国黄麻炭疽病病原菌的分离鉴定及系统发育分析[J]. 作物学报, 2022, 48(3): 770-777.

GUO Yan-Chun, YAO Jia-Yu, ZHANG Rong-Bin, CHEN Si-Yuan, HE Qing-Yao, TAO Ai-Fen, FANG Ping-Ping, QI Jian-Min, ZHANG Lie-Mei, ZHANG Li-Wu. Identification and phylogenetic analysis of the pathogen of jute anthracnose in China[J]. Acta Agronomica Sinica, 2022, 48(3): 770-777.

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扩增区域名称 Amplified region name	引物名称 Primer name	引物序列 Primer sequence (5°-3°)	退火温度 Annealing temperature (℃)
rDNA-ITS	ITS1	TCCGTAGGTGAACCTGCGG	55
rDNA-ITS	ITS4	TCCTCCGCTTATTGATATGC
LSU	NL1	GCATATCAATAAGCGGAGGAAAAG	57
LSU	NL4	GGTCCGTGTTTCAAGACGG

菌株名称 Strain	福黄麻3号病斑平均大小 Average size of Fuhuangma 3 lesions	菌株名称 Strain	福农5号病斑平均大小 Average size of Funong 5 lesions
福州地区Fuzhou region
GX19	11.70 a	GX19	16.40 a
XY13	10.30 ab	CS13	13.60 ab
CS13	9.80 ab	CS5	13.40 abc
CS5	9.70 ab	ZZ6	12.80 abc
CS3	9.50 ab	ZZ5	11.90 abcd
GX17	9.10 ab	GX17	11.70 abcde
GX9	9.00 ab	XY13	11.60 abcde
ZZ5	9.00 ab	GX10	10.90 bcde
GX10	7.90 ab	CS3	8.40 cde
ZZ6	7.80 ab	GX9	7.20 de
ZZ4	5.20 b	ZZ4	6.80 e
三明地区Sanming region
GX19	13.00 a	GX19	13.40 a
GX9	11.10 ab	GX9	12.90 a
ZZ5	10.60 bc	ZZ5	12.40 a
CS3	10.10 bcd	ZZ4	11.50 ab
XY13	9.30 bcde	XY13	11.40 ab
GX17	9.20 bcde	CS13	10.80 ab
ZZ4	8.70 cde	GX17	10.70 ab
ZZ6	8.60 cde	ZZ6	10.70 ab
CS13	8.10 def	CS3	9.10 bc
CS5	7.30 ef	CS5	7.00 c
GX10	6.40 f	GX10	6.90 c

中国黄麻炭疽病病原菌的分离鉴定及系统发育分析

Identification and phylogenetic analysis of the pathogen of jute anthracnose in China

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