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作物学报 ›› 2025, Vol. 51 ›› Issue (12): 3369-3376.doi: 10.3724/SP.J.1006.2025.52010

• 研究简报 • 上一篇    下一篇

基于“标准差系数加权法”的水稻全生育期耐盐碱鉴定与资源筛选

孙现军, 于太飞1, 胡正1, 申鑫萍1, 戈文艺2, 姜雪敏2, 王世佳2, 于思佳2, 武书羽2, 韩龙植1, 张辉1,*(), 姜奇彦1,3,*()   

  1. 1中国农业科学院作物科学研究所作物基因资源与育种全国重点实验室, 北京 100081
    2中国农业科学院作物科学研究所农业农村部粮食作物基因资源评价利用重点实验室, 北京 100081
    3国家盐碱地综合利用技术创新中心, 山东东营 257000
  • 收稿日期:2025-03-27 接受日期:2025-09-10 出版日期:2025-12-12 网络出版日期:2025-09-23
  • 通讯作者: *姜奇彦, E-mail: jiangqiyan@caas.cn;张辉, E-mail: zhanghui06@caas.cn
  • 基金资助:
    本研究由国家重点研发计划项目(2021YFD1200501);山东省重点研发计划(农业良种工程)项目(ZDYF2023LZGC001);中国农业科学院科技创新工程项目(ASTIP CAAS-ZDRW202407);中国农业科学院科技创新工程项目(01-ICS-02);中央级公益性科研院所基本科研业务费专项(Y2025JC23);江苏省现代作物生产协同创新中心和现代作物生产省部共建协同创新中心项目(CIC-MCP)

Assessment of salt-alkali tolerance throughout the rice growth period and germplasm screening based on the coefficient of standard deviation weighting method

SUN Xian-Jun, YU Tai-Fei1, HU Zheng1, SHEN Xin-Ping1, GE Wen-Yi2, JIANG Xue-Min2, WANG Shi-Jia2, YU Si-Jia2, WU Shu-Yu2, HAN Long-Zhi1, ZHANG Hui1,*(), JIANG Qi-Yan1,3,*()   

  1. 1State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    2Key Laboratory of Grain Crop Genetic Resources Evaluation and Utlization, Ministry of Agriculture and Rural Affairs, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying 257000, Shandong, China
  • Received:2025-03-27 Accepted:2025-09-10 Published:2025-12-12 Published online:2025-09-23
  • Contact: *E-mail: jiangqiyan@caas.cn;E-mail: zhanghui06@caas.cn
  • Supported by:
    National Key Research and Development Program of China(2021YFD1200501);Key Research and Development Program of Shandong Province (Agricultural Seed Improvement Project)(ZDYF2023LZGC001);Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(ASTIP CAAS-ZDRW202407);Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(01-ICS-02);Central Public-Interest Scientific Institution Basal Research Fund(Y2025JC23);Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry(CIC-MCP)

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摘要：

在盐碱地上种植适生的水稻种质资源是有效利用盐碱地的主要途径之一, 水稻全生育期耐盐碱鉴定评价对精准筛选耐盐碱种质资源起着关键作用。为全面评价水稻种质资源在0.5%盐碱处理下全生育期的耐盐碱能力, 本研究每隔7~10 d调查一次, 采用“标准差系数加权法”综合多次调查数据, 对水稻种质资源进行耐盐碱鉴定评价。该方法操作步骤如下: 首先将种质资源的耐盐等级重新赋值, 分别将1级、3级、5级、7级、9级的耐盐等级依次赋值为0、1、2、3、4; 然后以每次调查时材料间重新赋值的标准差为动态权重系数, 对水稻种质资源多次调查的耐盐等级进行加权平均计算; 最后依据加权平均值计算出各材料的盐害指数, 综合判定供试水稻种质资源的耐盐等级。利用上述鉴定方法对来自国内外的1200份水稻种质资源进行耐盐碱鉴定评价发现, 耐盐等级为1级、2级、3级、4级、5级的水稻种质资源数量依次为32份、437份、396份、301份和34份, 其中1级耐盐碱材料占比2.67%。结合0.3%盐碱胁迫下的水稻种质资源全生育期耐盐碱鉴定评价结果分析表明, “R223” “12-1819” “大粮317” “秭归糯” “恩恢1899” 5份水稻种质资源依据耐盐碱表型(0.5%盐碱处理下)和依据产量判定的耐盐等级(0.3%盐碱处理下)均为1级。筛选出的兼顾耐盐碱表型好和产量耐盐指数高的耐盐碱水稻资源, 为后续深入研究水稻耐盐碱机制以及培育耐盐碱水稻新品种提供了宝贵的种源创新基础。

关键词: 水稻种质资源, 标准差系数加权法, 盐害指数, 全生育期耐盐碱鉴定评价, 耐盐碱资源筛选

Abstract:

Cultivating suitable rice germplasm on saline-alkali land is a key strategy for the efficient utilization of such soils. Accurately evaluating salt-alkali tolerance throughout the entire growth period is essential for identifying tolerant germplasm. To comprehensively assess the salt-alkali tolerance of rice germplasm under 0.5% saline-alkali stress across the full growth period, this study conducted field surveys at 7-10 d intervals and applied the coefficient of standard deviation weighting method to integrate data from multiple assessments. The procedure involved three main steps: first, reassigning salt tolerance grades (original grades 1, 3, 5, 7, and 9 were converted to 0, 1, 2, 3, and 4, respectively); second, calculating the standard deviation of these reassigned values across different materials in each survey to serve as dynamic weighting coefficients; and third, computing a weighted average of salt tolerance scores for each germplasm, from which a salt damage index was derived to determine the final tolerance grade. Using this method, a total of 1,200 domestic and international rice germplasm resources were evaluated. The results showed that 32, 437, 396, 301, and 34 germplasm accessions fell into salt tolerance grades 1 through 5, respectively, with grade 1 (highest tolerance) accounting for 2.67%. When combined with results from a separate evaluation under 0.3% saline-alkali stress, five germplasm accessions—“R223”, “12-1819”, “Daliang 317”, “Ziguinuo”, and “Enhui 1899”—consistently exhibited grade 1 tolerance in both phenotypic (0.5%) and yield-based (0.3%) assessments. These elite salt-alkali tolerant rice germplasm resources, characterized by both strong phenotypic performance and high yield potential under saline-alkali stress, provide valuable genetic materials for future studies on the mechanisms of salt-alkali tolerance and for breeding new tolerant rice varieties.

Key words: rice germplasm resources, coefficient of standard deviation weighting method, salt damage index, identification and evaluation of salt-alkali tolerance throughout the growth period, screening of salt-alkali tolerant resources

图1

水稻种质资源供试材料的耐盐等级统计分析 A: 各调查时期材料的耐盐等级份数统计; B: 各调查时期材料耐盐等级的标准差。"

表1

基于盐害指数的水稻耐盐碱鉴定评价标准"

盐害指数
Salt damage index (S, %)		 耐盐级别
Tolerance grade		 耐盐性
Salt tolerance
S ≤ 20.0 1级 1 grade 高耐 High tolerance (HT)
20.0 < S ≤ 40.0 2级 2 grade 耐盐 Tolerance (T)
40.0 < S ≤ 60.0 3级 3 grade 中耐 Medium tolerance (MT)
60.0 < S ≤ 80.0 4级 4 grade 敏感 Sensitive (S)
80.0 < S ≤ 100.0 5级 5 grade 高敏 High sensitive (HS)

图2

水稻种质资源在不同盐碱胁迫处理下鉴定评价统计分析 A: 在0.5%盐碱胁迫下基于表型盐害指数统计; B 在0.3%盐碱胁迫下基于产量相对耐盐力统计。"

图3

基于“标准差系数加权法”对供试材料进行耐盐性鉴定评价结果统计"

表2

部分水稻种质资源在0.5%盐碱胁迫下调查指标统计分析"

种质名称
Name of germplasm		 不同调查时间耐盐等级
Salinity tolerance grade at different survey time points		 标准差系数加权法
Coefficient of standard deviation weighting method
07-06 07-12 07-18 07-24 08-01 08-10 08-21 08-31 09-12 盐害指数
Salt damage index (%)		 耐盐等级
Salinity tolerance grade
R223 1 1 1 1 1 1 3 5 5 16 1
12-1819 1 1 1 1 1 1 3 5 5 16 1
大粮317
Daliang 317		 1 1 1 1 1 1 3 5 7 19 1
秭归糯
Ziguinuo		 1 1 1 1 3 3 3 3 5 20 1
恩恢1899
Enhui 1899		 1 1 1 1 3 3 3 3 5 20 1
权重系数
Weight coefficient		 0.117 0.457 0.693 0.643 0.743 0.980 0.983 0.853 0.673
[1] 胡炎, 杨帆, 杨宁, 贾伟, 崔勇. 盐碱地资源分析及利用研究展望. 土壤通报, 2023, 54: 489-494.
Hu Y, Yang F, Yang N, Jia W, Cui Y. Analysis and prospects of saline-alkali land in China from the perspective of utilization. Chin J Soil Sci, 2023, 54: 489-494 (in Chinese with English abstract).
[2] 刘国兰, 陈之豪, 张珍, 王加红, 余新桥, 罗利军. 耐盐碱节水抗旱稻的种质创新. 上海农业学报, 2022, 38(4): 96-102.
Liu G L, Chen Z H, Zhang Z, Wang J H, Yu X Q, Luo L J. Germplasm innovation of water-saving and drought-resistance rice with saline-alkaline tolerance. Acta Agric Shanghai, 2022, 38(4): 96-102 (in Chinese with English abstract).
[3] 赵耕毛, 杨梦圆, 陈硕, 苏纪康, 吕慧琳, 贾慧昕, 刘兆普. 我国盐碱地治理: 现状、问题与展望. 南京农业大学学报, 2025, 48(1): 14-26.
Zhao G M, Yang M Y, Chen S, Su J K, Lyu H L, Jia H X, Liu Z P. Saline-alkali land management in China: current situation, problems and prospects. J Nanjing Agric Univ, 2025, 48(1): 14-26 (in Chinese with English abstract).
[4] 杨德卫, 张海峰, 余文权. 我国水稻种质资源创新研究与利用进展. 植物遗传资源学报, 2024, 25: 495-508.
doi: 10.13430/j.cnki.jpgr.20231029001
Yang D W, Zhang H F, Yu W Q. Progress on innovative research and utilization of rice germplasm resources in China. J Plant Genet Resour, 2024, 25: 495-508 (in Chinese with English abstract).
doi: 10.13430/j.cnki.jpgr.20231029001
[5] Yu J, Zhu C S, Xuan W, An H Z, Tian Y L, Wang B X, Chi W C, Chen G M, Ge Y W, Li J, et al. Genome-wide association studies identify OsWRKY 53 as a key regulator of salt tolerance in rice. Nat Commun, 2023, 14: 3550.
doi: 10.1038/s41467-023-39167-0
[6] Xu S B, Zheng J, Du H Q, Du X D, Li C, Duan Y X, Cai Y N, Wang J G, Liu H L, Yang L M, et al. GWAS combined with linkage analysis reveals major QTLs and candidate genes of salt tolerance in japonica rice seedlings. Front Plant Sci, 2024, 15: 1462856.
[7] 周汝倫, 侯家龙, 方宗熙, 王明珍, 宋景芝, 刘惠令. 耐盐水稻品种选育初报. 中国农业科学, 1983, 16(5): 7-13.
Zhou R L, Hou J L, Fang T C, Wang M Z, Song J Z, Liu H L. First report on breeding of salt-tolerant rice plants. Sci Agric Sin, 1983, 16(5): 7-13 (in Chinese with English abstract).
[8] 吴荣生, 王志霞, 蒋荷, 王根来, 顾平, 陈炳泉. 太湖流域稻种资源耐盐性筛选鉴定. 江苏农业科学, 1989, 17(1): 8-9.
Wu R S, Wang Z X, Jiang H, Wang G L, Gu P, Chen B Q. Screening and identification of salt tolerance of rice germplasm resources in Taihu Lake Basin. Jiangsu Agric Sci, 1989, 17(1): 8-9 (in Chinese).
[9] 王才林, 张亚东, 赵凌, 路凯, 朱镇, 陈涛, 赵庆勇, 姚姝, 周丽慧, 赵春芳, 等. 耐盐碱水稻研究现状、问题与建议. 中国稻米, 2019, 25(1): 1-6.
Wang C L, Zhang Y D, Zhao L, Lu K, Zhu Z, Chen T, Zhao Q Y, Yao S, Zhou L H, Zhao C F, et al. Research status, problems and suggestions on salt-alkali tolerant rice. China Rice, 2019, 25(1): 1-6 (in Chinese with English abstract).
doi: 10.3969/j.issn.1006-8082.2019.01.001
[10] 孙现军, 姜奇彦, 胡正, 张惠媛, 徐长兵, 邸一桓, 韩龙植, 张辉. 水稻资源全生育期耐盐性鉴定筛选. 作物学报, 2019, 45: 1656-1663.
doi: 10.3724/SP.J.1006.2019.92012
Sun X J, Jiang Q Y, Hu Z, Zhang H Y, Xu C B, Di Y H, Han L Z, Zhang H. Screening and identification of salt-tolerant rice germplasm in whole growth period. Acta Agron Sin, 2019, 45: 1656-1663 (in Chinese with English abstract).
[11] 王佳丽, 黄贤金, 钟太洋, 陈志刚. 盐碱地可持续利用研究综述. 地理学报, 2011, 66: 673-684.
Wang J L, Huang X J, Zhong T Y, Chen Z G. Review on sustainable utilization of salt-affected land. Acta Geogr Sin, 2011, 66: 673-684 (in Chinese with English abstract).
doi: 10.11821/xb201105010
[12] 孙明法, 严国红, 王爱民, 朱国永, 唐红生, 何冲宵, 任仲玲, 刘凯, 张桂云, 施伟, 等. 水稻耐盐育种研究进展. 大麦与谷类科学, 2017, 34(4): 1-9.
Sun M F, Yan G H, Wang A M, Zhu G Y, Tang H S, He C X, Ren Z L, Liu K, Zhang G Y, Shi W, et al. Research progress on the breeding of salt-tolerant rice varieties. Barley Cereal Sci, 2017, 34(4): 1-9 (in Chinese with English abstract).
[13] Qin H, Li Y X, Huang R F. Advances and challenges in the breeding of salt-tolerant rice. Int J Mol Sci, 2020, 21: 8385.
doi: 10.3390/ijms21218385
[14] 潘大建, 李晨, 范芝兰, 孙炳蕊, 陈文丰, 江立群, 张静, 吕树伟, 刘清, 毛兴学. 广东省农业科学院水稻种质资源研究60年: 成就与展望. 广东农业科学, 2020, 47(11): 18-31.
Pan D J, Li C, Fan Z L, Sun B R, Chen W F, Jiang L Q, Zhang J, Lyu S W, Liu Q, Mao X X. Sixty years’ researches on rice germplasm resources of Guangdong academy of agricultural sciences: achievements and prospects. Guangdong Agric Sci, 2020, 47(11): 18-31 (in Chinese with English abstract).
[15] 赵记伍, 雷传松, 刘永权, 张露, 成云峰, 王晓玲. 海稻86萌发期耐盐碱性特征初探. 中国稻米, 2018, 24(3): 87-92.
doi: 10.3969/j.issn.1006-8082.2018.03.019
Zhao J W, Lei C S, Liu Y Q, Zhang L, Cheng Y F, Wang X L. Primary exploration on saline-alkali tolerance of Haidao 86 in germination period. China Rice, 2018, 24(3): 87-92 (in Chinese with English abstract).
doi: 10.3969/j.issn.1006-8082.2018.03.019
[16] Phan N T H, Van Pham C, Tang H T, Van Nguyen L, Nguyen L V, Bertin P. Integration of genome-wide association studies reveal loci associated with salt tolerance score of rice at the seedling stage. J Appl Genet, 2023, 64: 603-614.
doi: 10.1007/s13353-023-00775-7 pmid: 37555917
[17] 刘艳, 王宝祥, 邢运高, 陈庭木, 徐波, 杨波, 孙志广, 刘金波, 迟铭, 李健, 等. 水稻品种资源苗期耐盐性评价指标分析. 江苏农业科学, 2021, 49(17): 75-79.
Liu Y, Wang B X, Xing Y G, Chen T M, Xu B, Yang B, Sun Z G, Liu J B, Chi M, Li J, et al. Analysis of evaluation indices for salt tolerance of rice germplasm resources at seedling stage. Jiangsu Agric Sci, 2021, 49(17): 75-79 (in Chinese).
[18] 程海涛, 苏展, 曹萍, 郭玉华. NaCl和Na2CO3胁迫对水稻籼粳杂交后代群体发芽与幼苗生育的影响. 沈阳农业大学学报, 2010, 41: 73-77.
Cheng H T, Su Z, Cao P, Guo Y H. Effects of NaCl and Na2CO3stress to indica × japonica hybrid in germination and early seedling period. J Shenyang Agric Univ, 2010, 41: 73-77 (in Chinese with English abstract).
[19] 祁栋灵, 张三元, 曹桂兰, 阮仁超, 孙明茂, 张艳蕊, 周庆阳, 韩龙植. 水稻发芽期和幼苗前期耐碱性的鉴定方法研究. 植物遗传资源学报, 2006, 7: 74-80.
Qi D L, Zhang S Y, Cao G L, Ruan R C, Sun M M, Zhang Y R, Zhou Q Y, Han L Z. Studies on screening methods for alkaline tolerance at germination period and early seedling stage in rice. J Plant Genet Resour, 2006, 7: 74-80 (in Chinese with English abstract).
[20] 耿雷跃, 马小定, 崔迪, 张启星, 韩冰, 韩龙植. 水稻全生育期耐盐性鉴定评价方法研究. 植物遗传资源学报, 2019, 20: 267-275.
doi: 10.13430/j.cnki.jpgr.20180815003
Geng L Y, Ma X D, Cui D, Zhang Q X, Han B, Han L Z. Identification and evaluation method for saline tolerance in rice during the whole growth stage. J Plant Genet Resour, 2019, 20: 267-275 (in Chinese with English abstract).
doi: 10.13430/j.cnki.jpgr.20180815003
[21] 潘晓飚, 黄善军, 陈凯, 孟丽君, 徐建龙. 大田全生育期盐水灌溉胁迫筛选水稻耐盐恢复系. 中国水稻科学, 2012, 26: 49-54.
Pan X B, Huang S J, Chen K, Meng L J, Xu J L. Selection of rice restorer lines with salinity tolerance through salt solution irrigation over whole growth stage under field conditions. Chin J Rice Sci, 2012, 26: 49-54 (in Chinese with English abstract).
[22] 周玉杰, 贺治洲, 林秋云, 胡伟, 龙开意, 林越辉, 谢振宇. 热带水稻种质资源全生育期耐盐性鉴定与评价. 南方农业学报, 2023, 54: 1944-1952.
Zhou Y J, He Z Z, Lin Q Y, Hu W, Long K Y, Lin Y H, Xie Z Y. Identification and evaluation for salt tolerance of tropical rice germplasm resources in whole growth period. J South Agric, 2023, 54: 1944-1952 (in Chinese with English abstract).
[23] Khan M A, Abdullah Z. Salinity-sodicity induced changes in reproductive physiology of rice (Oryza sativa) under dense soil conditions. Environ Exp Bot, 2003, 49: 145-157.
doi: 10.1016/S0098-8472(02)00066-7
[24] 中华人民共和国农业行业标准. 水稻耐盐性鉴定技术: NY/T 3692-2020, 2020.
Agricultural Industry Standards of the People’s Republic of China. Technical Code of Practice for Identification of Salt Tolerance in Rice: NY/T 3692-2020, 2020 (in Chinese).
[25] Zhou H, Wang X, Tang S Y. Global dynamics of non-smooth Filippov pest-natural enemy system with constant releasing rate. Math Biosci Eng, 2019, 16: 7327-7361.
doi: 10.3934/mbe.2019366 pmid: 31698615
[26] Calmon M A, Jones J W, Shinde D, Specht J E. Estimating parameters for soil water balance models using adaptive simulated annealing. Appl Eng Agric, 1999, 15: 703-713.
doi: 10.13031/2013.5841
[27] Guan X D, Liu G H, Huang C, Meng X L, Liu Q S, Wu C S, Ablat X, Chen Z R, Wang Q. An open-boundary locally weighted dynamic time warping method for cropland mapping. ISPRS Int J Geo-Inf, 2018, 7: 75.
doi: 10.3390/ijgi7020075
[28] Bi L N, Wally O, Hu G P, Tenuta A U, Kandel Y R, Mueller D S. A transformer-based approach for early prediction of soybean yield using time-series images. Front Plant Sci, 2023, 14: 1173036.
[29] 王洋, 张瑞, 刘永昊, 李荣凯, 葛建飞, 邓仕文, 张徐彬, 陈英龙, 韦还和, 戴其根. 水稻对盐胁迫的响应及耐盐机理研究进展. 中国水稻科学, 2022, 36: 105-117.
doi: 10.16819/j.1001-7216.2022.210609
Wang Y, Zhang R, Liu Y H, Li R K, Ge J F, Deng S W, Zhang X B, Chen Y L, Wei H H, Dai Q G. Rice response to salt stress and research progress in salt tolerance mechanism. Chin J Rice Sci, 2022, 36: 105-117 (in Chinese with English abstract).
doi: 10.16819/j.1001-7216.2022.210609
[30] 徐远逍, 王钰媛, 杨益善. 不同水稻不育系对盐胁迫的生理响应及耐盐性评价. 江苏农业科学, 2024, 52(16): 101-109.
Xu Y X, Wang Y Y, Yang Y S. Physiological response and salt tolerance evaluation of different rice male sterile lines to salt stress. Jiangsu Agric Sci, 2024, 52(16): 101-109 (in Chinese).
[31] 周政, 李宏, 孙勇, 黄道强, 朱苓华, 卢德城, 李康活, 徐建龙, 周少川, 黎志康. 高产、抗旱和耐盐选择对水稻产量相关性状的影响. 作物学报, 2010, 36: 1725-1735.
doi: 10.3724/SP.J.1006.2010.01725
Zhou Z, Li H, Sun Y, Huang D Q, Zhu L H, Lu D C, Li K H, Xu J L, Zhou S C, Li Z K. Effect of selection for high yield, drought and salinity tolerances on yield-related traits in rice (Oryza sativa L.). Acta Agron Sin, 2010, 36: 1725-1735 (in Chinese with English abstract).
[32] 冯培媛, 董悦, 焦紫岚, 陈敏, 孔维儒, 冉杰, 李培富, 田蕾. 粳稻种质苗期耐盐性综合评价与全基因组关联分析. 河南农业科学, 2024, 53(7): 1-9.
Feng P Y, Dong Y, Jiao Z L, Chen M, Kong W R, Ran J, Li P F, Tian L. Comprehensive evaluation of salt tolerance and genome- wide association study of japonica rice germplasms at seedling stage. J Henan Agric Sci, 2024, 53(7): 1-9 (in Chinese with English abstract).
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