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作物学报 ›› 2026, Vol. 52 ›› Issue (4): 959-981.doi: 10.3724/SP.J.1006.2026.53074

• 综述 •    下一篇

sRNA的生物合成和作用机制及其对种子发育和休眠与萌发调控的研究进展

宋松泉1,2,*(), 唐翠芳1,3, 梁裕荣1, 程红焱2, 王伟青2   

  1. 1广州市业坤医药集团有限公司, 广东广州 510650
    2中国科学院植物研究所, 北京 100093
    3广州常春藤种业科技有限公司, 广东广州 510650
  • 收稿日期:2025-10-09 接受日期:2025-12-16 出版日期:2026-04-12 网络出版日期:2025-12-22
  • 通讯作者: *宋松泉, E-mail: sqsong2019@163.com
  • 基金资助:
    国家自然科学基金项目(32470357);郴州国家可持续发展议程创新示范区建设省级专项(2022sfq06)

Research progress in biosynthesis and action mechanism of sRNAs and their regulation on seed development, dormancy and germination

Song Song-Quan1,2,*(), Tang Cui-Fang1,3, Liang Yu-Rong1, Cheng Hong-Yan2, Wang Wei-Qing2   

  1. 1Guangzhou Yekun Pharmaceutical Group Co., Ltd., Guangzhou 510650, Guangdong, China
    2Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    3Guangzhou Ivy Seed Industry Technology Co., Ltd., Guangzhou 510650, Guangdong, China
  • Received:2025-10-09 Accepted:2025-12-16 Published:2026-04-12 Published online:2025-12-22
  • Contact: *E-mail: sqsong2019@163.com
  • Supported by:
    National Natural Science Foundation of China(32470357);Provincial Special Project of Chenzhou National Sustainable Development Agenda Innovation Demonstration Zone Construction(2022sfq06)

摘要:

种子的发育、休眠和萌发是植物生命周期中的重要过程, 受许多遗传因素和环境因子的调控。小RNAs(sRNAs)是一组由19~24个核苷酸组成的非编码RNA分子, 调控编码转录因子和关键调节蛋白的基因表达, 在植物和动物的形态发生、生长、发育以及对生物和非生物胁迫的反应中发挥着重要的作用。尽管植物sRNA的部分调控机制尚不明确, 但已有的证据表明它们显著调控种子的发育、休眠和萌发。本文综述了近年来植物sRNA的研究进展, 主要包括sRNA的生物发生(生物合成)和作用机制, 以及它们对种子发育、休眠和萌发的调控作用, 并提出了在本领域需要进一步研究的科学问题, 试图为深入研究sRNAs在调控种子发育、休眠和萌发中的分子机制, 以及提高种子的质量与产量和萌发活力提供参考。

关键词: 基因沉默, 调控, 种子发育、休眠与萌发, 小RNA, 翻译抑制

Abstract:

The development, dormancy and germination of seeds are critical processes in the plant life cycle, and are regulated by various genetic factors and environmental cues. Small RNAs (sRNAs) are a group of non-coding RNA molecules consisting of 19-24 nucleotides, and regulate the expression of genes encoding transcription factors and key regulatory proteins. They play important roles in morphogenesis, growth, development, and response on biotic and abiotic stresses in both plants and animals. Although some of the regulation mechanisms of plant sRNAs remain unclear, existed evidences indicate their significant regulatory roles in seed development, dormancy, and germination. In the present paper, the research progresses of plant sRNAs in recent years were reviewed, mainly including sRNA biogenesis (biosynthesis) and action mechanism, as well as their regulatory roles in seed development, dormancy, and germination. Additionally, we have highlighted key scientific issues requiring further investigation in this field. The aim is to deepen our understanding of sRNA-mediated molecular mechanisms in these processes, thereby providing insights into improving seed quality, yield, and germination vigor.

Key words: gene silencing, regulation, seed development, dormancy and germination, small RNAs, translation repression

图1

影响种子发育和萌发的主要生理过程和分子事件的模式图 图的左边和右边分别代表种子发育和萌发的主要生理过程和分子事件, 中间区域表示种子的静止过程(用时间表示)。值得注意的是, 萌发能力、脱水耐性和休眠特性是在种子发育过程中同时形成的。种子的休眠释放和萌发均受植物激素、基因转录与转录后、表观遗传和环境因子的调控(图中未表明)。蓝色虚线表示不同发育阶段的水分含量变化。每个生理过程或分子事件下面的长条形方框表示该过程或该事件进行的起始时间与变化趋势。AFL: ABI3、FUS3 (FUSCA3)和LEC2 (LEAFY COTYLEDON 2)亚家族; DOG1: 萌发延迟1; TF: 转录因子; W: 星期; Y: 年。"

图2

典型的植物sRNA的生物发生和作用模式 hc-siRNA主要是通过Pol IV、CLSY、RDR2和DCL3的作用产生。hc-siRNA与AGO4/AGO6蛋白形成RISC, 并通过DRM2在Pol V转录位点指导DNA甲基化。hc-siRNA及其靶标转录起始点上的模糊箭头表示尚未被充分表征的Pol IV/Pol V转录起始位点。miRNA主要通过Pol II、DCL1、HYL1和SE的作用产生。miRNA优先与AGO1形成RISC; miRISC可以指导转录本的切割、介导翻译抑制或触发phasiRNA生物发生。phasiRNA由Pol II、RDR6和DCL4/DCL5产生。参与生物发生或作用的其他蛋白在正文中被描述。AGO: Argonaute; CLSY: CLASSY; D-body: 核切割体; DCL: Dicer-like; DRM2: 结构域重排的甲基化酶2; ER: 内质网; hc-siRNA: 异染色质小干扰RNA; HEN1: HUA增强子1; HYL1: HYPONASTIC LEAVES 1; MIR: 微小RNA基因; miRISC: miRNA诱导的沉默复合物; miRNA: 微小RNA; PHAS: 相位次级小干扰RNA位点; phasiRNA: 相位次级小干扰RNA; Pol II: RNA聚合酶II; Pol IV: RNA聚合酶IV; Pol V: RNA聚合酶V; RDR: RNA依赖的RNA聚合酶; RISC: RNA诱导的沉默复合物; SE: SERRATE; SGS3: 基因沉默抑制因子3; SHH1: SAWADEE同源结构域同源物1; sRNA: 小RNA。"

图3

sRNA调控种子形成、发育、休眠和萌发的示意图 小RNAs (sRNAs)包括微小RNAs (miRNAs, miRs)和小干扰RNAs (siRNAs), 通过转录后基因沉默或翻译抑制调控编码转录因子和关键调节蛋白的基因表达, 并通过与植物激素、DOG1和表观遗传因子相互作用在种子形成、发育(包括种子大小)、休眠和萌发的调控中发挥重要作用。黑色虚线表示种子形成和发育与种子休眠和萌发的相互关系。种子形成与发育是种子休眠和萌发的前提, 休眠种子必须在干藏、冷湿和植物激素处理解除休眠后才能萌发。→和?分别表示促进和抑制。ABA: 脱落酸; ABF: ABA反应元件结合因子; ABI3: ABA不敏感3; ABRE: ABA反应元件; ABRG: ABA反应基因; AGO: Argonaute; AHG1/3: ABA过敏感萌发1/3; AREB: ABRE结合蛋白; ARF10: 生长素反应因子10; At: 拟南芥; DCL1: DICER-LIKE 1; DML3: DEMETER-LIKE PROTEIN 3; DOG1: 萌发延迟1; FUS3: FUSCA3; GA: 赤霉素; GA20ox: GA 20氧化酶; GA3ox: GA 3氧化酶; GAMYB: 赤霉素诱导的MYB转录因子; GARE: GA反应元件; GARG: GA反应基因; GID1: 赤霉素不敏感矮化1; GRF: 生长调节因子; HEN1: HUA ENHANCER 1; HYL1: HYPONASTIC LEAVES 1; IPA1: Ideal Plant Architecture 1; KO: 内根-贝壳杉烯氧化酶; LEC2: leafy cotyledon 2; MIR: microRNA基因; NCED: 9-顺式环氧类胡萝卜素双加氧酶; nt: 核苷酸; Os: 水稻; PCD: 细胞程序性死亡; phasiRNA: 相位次级小干扰RNA; pol II: RNA聚合酶II; PP2C: 蛋白磷酸酶2C; pre-miRNA: 前体miRNA; pri-miRNA: 初级miRNA; PTGS: 转录后基因沉默; PYL: pyrabactin resistance 1/pyrabactin resistance 1-like/regulatory components of ABA receptor; SLR1: SLENDER RICE 1; SnRK2: SNF1 (蔗糖非发酵-1)相关的蛋白激酶2; SPL: SQUAMOSA PROMOTER BINDING PROTEIN-LIKE; TRRE: 翻译抑制; WSGAR: 小麦种子萌发相关的RNA基因; ZEP: 玉米黄质环氧化酶。"

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