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作物学报 ›› 2026, Vol. 52 ›› Issue (10): 2875-2885.doi: 10.3724/SP.J.1006.2026.64047

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

马铃薯MDH基因家族的全基因组鉴定及表达分析

崔一凡(), 孙晓彤, 石瑛()   

  1. 东北农业大学农学院, 黑龙江哈尔滨 150030
  • 收稿日期:2026-04-10 接受日期:2026-07-15 出版日期:2026-10-12 网络出版日期:2026-07-22
  • 通讯作者: 石瑛, E-mail: yshi@neau.edu.cn
  • 作者简介:崔一凡, E-mail: yfcui0321@163.com
  • 基金资助:
    财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-09)

Genome-wide identification and expression analysis of the MDH gene family in potato (Solanum tuberosum L.)

Cui Yi-Fan(), Sun Xiao-Tong, Shi Ying()   

  1. College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
  • Received:2026-04-10 Accepted:2026-07-15 Published:2026-10-12 Published online:2026-07-22
  • Contact: Shi Ying, E-mail: yshi@neau.edu.cn
  • Supported by:
    China Agriculture Research System of MOF and MARA(CARS-09)

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摘要：

苹果酸脱氢酶(MDH; EC1.1.1.37)广泛存在于植物中, 能利用还原型烟酰胺腺嘌呤二核苷酸或还原型烟酰胺腺嘌呤二核苷酸磷酸催化苹果酸与草酰乙酸之间的转化, 参与三羧酸循环过程, 同时参与调控植物的生长发育及逆境响应等多种生理过程。本研究采用生物信息学方法, 在马铃薯基因组中鉴定出8个MDH家族成员, 依据染色体位置被命名为StMDH1~StMDH8, 并进一步将其划分为5个亚家族。理化性质分析显示, StMDHs编码蛋白的分子量介于30.11~43.85 kD之间, 等电点范围为5.91~9.00; 亚细胞定位预测表明, 这些蛋白主要分布于叶绿体和线粒体中。基因结构分析发现, 同一亚家族成员具有相似的外显子数目和保守的Motif组成。基于公开转录组数据的表达谱分析显示, 多数StMDHs基因对非生物胁迫及激素处理均有响应, 其中StMDH2对盐胁迫表现出最为显著的诱导表达模式。进一步通过酿酒酵母异源表达体系验证, StMDH2的过表达能够显著提高酵母细胞的耐盐性。本研究丰富了马铃薯MDH基因家族的理论认知, 为马铃薯抗逆分子育种提供了潜在的基因资源和候选靶点。

关键词: 马铃薯, MDH, 全基因组鉴定, 非生物胁迫, 盐胁迫

Abstract:

Malate dehydrogenase (MDH; EC1.1.1.37) is widely distributed in plants and catalyzes the interconversion of malate and oxaloacetate using NADH or NADPH as cofactors. MDH plays a crucial role in the tricarboxylic acid (TCA) cycle and is involved in regulating various physiological processes, including plant growth, development, and stress responses. In this study, eight MDH family members were identified in the potato genome using bioinformatics approaches. These genes were named StMDH1 to StMDH8 according to their chromosomal locations and were further classified into five subfamilies. Analysis of physicochemical properties showed that the predicted StMDH proteins ranged from 30.11 to 43.85 kD in molecular weight, with isoelectric points ranging from 5.91 to 9.00. Subcellular localization prediction indicated that these proteins are mainly localized in chloroplasts and mitochondria. Gene structure analysis showed that members within the same subfamily had similar exon numbers and conserved motif compositions. Expression profiling based on publicly available transcriptome data revealed that most StMDH genes responded to abiotic stresses and hormone treatments, with StMDH2 showing the strongest salt stress-induced expression pattern. Furthermore, heterologous expression in Saccharomyces cerevisiae confirmed that overexpression of StMDH2 significantly enhanced salt tolerance in yeast cells. This study expands our understanding of the potato MDH gene family and provides potential genetic resources and candidate targets for the molecular breeding of stress-resistant potato varieties.

Key words: Solanum tuberosum L., MDH, genome-wide identification, abiotic stress, salt stress

附表1

58个MDH基因的基本信息"

基因名称
Gene name		 基因ID
Gene ID		 ORF长度
ORF length (bp)		 蛋白质长度
Protein length (aa)		 等电点
Isoelectric point		 分子量
Molecular weight (kD)		 亚细胞定位
Subcellular localization
StMDH1 Soltu.DM.01G031350 999 332 7.62 35.69 叶绿体Chloroplast
StMDH2 Soltu.DM.01G045790 1074 357 8.10 37.63 叶绿体, 线粒体
Chloroplast, mitochondrion
StMDH3 Soltu.DM.02G007400 873 290 6.42 30.11 线粒体Mitochondrion
StMDH4 Soltu.DM.03G030110 1239 412 8.04 43.28 叶绿体, 线粒体
Chloroplast, mitochondrion
StMDH5 Soltu.DM.07G023810 1041 346 9.00 36.12 叶绿体, 线粒体
Chloroplast, mitochondrion
StMDH6 Soltu.DM.09G026740 999 332 5.91 35.43 叶绿体Chloroplast
StMDH7 Soltu.DM.09G027890 1239 412 8.69 43.85 叶绿体, 线粒体
Chloroplast, mitochondrion
StMDH8 Soltu.DM.12G030010 1026 341 8.90 35.67 叶绿体, 线粒体
Chloroplast, mitochondrion
AtMDH1 AT1G04410 999 332 6.11 35.57 叶绿体Chloroplast
AtMDH2 AT1G53240 1026 341 8.54 35.80 线粒体Mitochondrion
AtMDH3 AT2G22780 1065 354 8.11 37.47 叶绿体, 线粒体
Chloroplast, mitochondrion
AtMDH4 AT3G15020 1026 341 8.30 35.88 叶绿体, 线粒体
Chloroplast, mitochondrion
AtMDH5 AT3G47520 1212 403 8.66 42.40 叶绿体, 线粒体
Chloroplast, mitochondrion
AtMDH6 AT5G09660 1091 363 9.16 38.74 叶绿体, 线粒体
Chloroplast, mitochondrion
AtMDH7 AT5G43330 999 332 6.33 35.68 叶绿体Chloroplast
AtMDH8 AT5G58330 1332 443 5.81 48.32 叶绿体Chloroplast
AtMDH9 AT5G56720 1020 339 5.75 36.87 叶绿体Chloroplast
GmMDH1 Glyma.01G197700 873 290 6.21 30.90 过氧化物酶体Peroxisome
GmMDH2 Glyma.01G210400 1062 353 6.25 38.20 叶绿体Chloroplast
GmMDH3 Glyma.02G005500 1002 333 5.91 35.50 细胞质Cytoplasm
GmMDH4 Glyma.05G026300 1245 414 6.47 43.60 叶绿体Chloroplast
GmMDH5 Glyma.05G058100 1053 350 6.51 37.80 叶绿体Chloroplast
GmMDH6 Glyma.06G231500 1041 346 8.22 36.00 线粒体Mitochondrion
GmMDH7 Glyma.07G185400 1074 357 8.52 37.50 过氧化物酶体Peroxisome
GmMDH8 Glyma.08G063800 1074 357 8.52 37.40 细胞质Cytoplasm
GmMDH9 Glyma.10G006500 990 329 5.91 35.20 细胞质Cytoplasm
GmMDH10 Glyma.10G197700 1314 437 6.18 47.70 叶绿体Chloroplast
GmMDH11 Glyma.11G031600 690 229 8.79 24.50 叶绿体Chloroplast
GmMDH12 Glyma.11G043900 1065 354 7.55 37.40 过氧化物酶体Peroxisome
GmMDH13 Glyma.12G159300 1041 346 8.23 36.10 线粒体Mitochondrion
GmMDH14 Glyma.13G104800 1125 374 6.13 41.00 细胞质Cytoplasm
GmMDH15 Glyma.17G100600 1233 410 8.11 43.10 叶绿体Chloroplast
GmMDH16 Glyma.17G140600 1053 350 6.55 37.70 叶绿体Chloroplast
GmMDH17 Glyma.20G192200 1278 425 6.34 46.60 叶绿体Chloroplast
OsMDH1 LOC_Os01g46070 1023 640 8.74 35.46 叶绿体, 线粒体Chloroplast, mitochondrion
OsMDH2 LOC_Os01g61380 1191 396 7.63 41.79 叶绿体, 线粒体
Chloroplast, mitochondrion
OsMDH3 LOC_Os02g01510 1179 392 6.74 42.72 细胞质Cytoplasm
OsMDH4 LOC_Os03g56280 1065 654 8.13 37.02 叶绿体, 线粒体
Chloroplast, mitochondrion
OsMDH5 LOC_Os04g46560 1059 352 6.72 38.30 叶绿体Chloroplast
OsMDH6 LOC_Os05g49880 1023 340 8.22 35.44 叶绿体, 线粒体
Chloroplast, mitochondrion
OsMDH7 LOC_Os06g01590 1083 360 7.90 38.72 细胞质Cytoplasm
OsMDH8 LOC_Os07g43700 1215 404 9.00 42.22 叶绿体, 线粒体
Chloroplast, mitochondrion
OsMDH9 LOC_Os08g33720 1194 397 7.02 41.54 叶绿体, 线粒体
Chloroplast, mitochondrion
OsMDH10 LOC_Os08g44810 1302 433 6.96 47.01 叶绿体Chloroplast
OsMDH11 LOC_Os10g33800 999 332 5.75 35.57 叶绿体Chloroplast
OsMDH12 LOC_Os12g43630 1071 356 8.09 37.39 叶绿体, 线粒体
Chloroplast, mitochondrion
SlMDH1 Solyc01g090710 1005 334 6.46 35.88 细胞质Cytoplasm
SlMDH2 Solyc01g106480 1074 357 8.10 37.63 过氧化物酶体Peroxisome
SlMDH3 Solyc02g063490 1059 352 8.13 36.85 叶绿体Chloroplast
SlMDH4 Solyc03g071590 1404 467 7.89 51.66 叶绿体Chloroplast
SlMDH5 Solyc03g115990 1239 412 8.34 43.19 叶绿体Chloroplast
SlMDH6 Solyc07g062650 1041 346 8.73 36.08 线粒体Mitochondrion
SlMDH7 Solyc08g007420 1044 347 5.36 37.64 细胞质Cytoplasm
SlMDH8 Solyc08g078850 1053 350 6.31 37.70 叶绿体Chloroplast
SlMDH9 Solyc09g090140 999 332 5.91 35.38 细胞质Cytoplasm
SlMDH10 Solyc09g091070 897 298 5.35 31.81 细胞质Cytoplasm
SlMDH11 Solyc11g007990 1329 442 6.23 48.44 叶绿体Chloroplast
SlMDH12 Solyc12g014180 1029 342 8.90 35.65 线粒体Mitochondrion

图1

马铃薯、番茄、拟南芥、水稻和大豆中MDH基因家族的系统发育进化树"

图2

马铃薯MDH基因结构分析"

图3

MDH基因的共线性分析 A: 马铃薯MDH基因的共线性分析, 其中红色代表重复的基因对; B: 马铃薯与番茄、拟南芥、水稻、大豆MDH基因的共线性分析, 彩色连线代表重复的基因对。"

图4

MDH基因家族保守基序分析"

图5

MDH基因家族启动子顺式作用元件预测 图中数字表示各基因启动子区域中特定顺式作用元件的拷贝数。"

图6

马铃薯MDH基因家族在不同组织器官及非生物胁迫与激素处理下的表达分析 A: MDH基因在马铃薯不同组织/器官的表达谱分析; B: 马铃薯MDH基因在非生物胁迫下的表达谱分析; C: 马铃薯MDH基因在激素处理下的表达谱分析。"

图7

StMDH2基因的克隆及在酿酒酵母异源表达中的耐盐功能验证 A: pYES2-StMDH2重组载体示意图, 括号内的数字表示各酶切位点或元件在质粒上的位置; B: 在酿酒酵母中异源表达StMDH2基因的耐盐功能验证。"

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