OsST41调控水稻苗期耐盐性的功能分析

doi:10.3724/SP.J.1006.2026.52023

摘要/Abstract

摘要： 水稻是我国第一大粮食作物，其产量易受土壤盐碱化影响。本课题组前期通过全基因组关联分析(GWAS)定位到水稻耐盐候选基因OsST41。本研究克隆了OsST41 (Os07g0598200)基因，通过生物信息学分析发现该基因编码序列全长747 bp，编码248个氨基酸，OsST41蛋白含有F-box保守结构域，并与拟南芥中调控非生物胁迫响应的EDL3蛋白同源。亚细胞定位结果表明，OsST41蛋白定位于细胞核。qRT-PCR分析显示，OsST41基因在水稻的各生育时期均有表达，并于苗期盐胁迫后在叶片中的表达量显著升高。利用CRISPR/Cas9基因编辑技术构建了OsST41基因敲除突变体。耐盐性分析发现，OsST41基因敲除突变体耐盐性显著下降，盐胁迫条件下突变体的Na?/K?比率显著高于野生型。生理指标检测结果发现，盐胁迫下突变体丙二醛含量显著高于野生型，超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性和脯氨酸(Pro)含量均低于野生型。qRT-PCR结果显示，抗氧化相关酶编码基因OsALDH22A1和OsGPX5的表达水平在突变体中显著降低。这些结果表明，突变体抗氧化系统受损，活性氧(ROS)清除能力减弱。本研究初步揭示了OsST41基因参与调控水稻耐盐性，为进一步解析水稻OsST41响应盐胁迫的分子调控机制奠定了基础。

关键词: 盐胁迫, 水稻, 生理指标, 亚细胞定位, OsST41

Abstract:

Rice is the most widely cultivated grain crop in China, and its yield is highly susceptible to soil salinization. In our previous study, a candidate gene associated with salt tolerance in rice, OsST41, was identified through a genome-wide association study (GWAS). In the present study, we cloned the OsST41 (Os07g0598200) gene. Bioinformatic analysis showed that the gene has a full-length coding sequence of 747 bp and encodes a 248-amino-acid protein containing a conserved F-box domain. The OsST41 protein shares homology with the EDL3 protein in Arabidopsis thaliana, which is known to be involved in the regulation of abiotic stress responses. Subcellular localization analysis revealed that OsST41 is localized in the nucleus. qRT-PCR analysis indicated that OsST41 is expressed at all developmental stages of rice, and its expression in leaves is significantly upregulated under salt stress at the seedling stage. To investigate the function of OsST41 in salt tolerance, we generated a knockout mutant using CRISPR/Cas9 gene-editing technology. The salt tolerance of the OsST41 knockout mutant was significantly reduced, and the Na⁺/K⁺ ratio under salt stress was markedly higher than that of the wild type. Physiological measurements showed that, under salt stress, the mutant had significantly higher malondialdehyde (MDA) content and lower activities of superoxide dismutase (SOD), catalase (CAT), and proline (Pro) content compared to the wild type. Furthermore, qRT-PCR analysis revealed that the expression levels of antioxidant-related genes OsALDH22A1 and OsGPX5 were significantly downregulated in the mutant. These results suggest that the antioxidant defense system is impaired in the mutant, leading to reduced reactive oxygen species (ROS) scavenging capacity. Collectively, our findings provide preliminary evidence that OsST41 plays a positive regulatory role in rice salt tolerance, laying a foundation for further exploration of its molecular mechanisms in response to salt stress.

Key words: salt stress, rice, physiological indicators, subcellular localization, OsST41

覃奕琰, 付瑶, 苏畅, 李娜, 徐静茹, 程笑然, 张琪, 赵明辉. OsST41调控水稻苗期耐盐性的功能分析[J]. 作物学报, 0, (): 0-.

Qin Yi-Yan, Fu Yao, Su Chang, Li Na, Xu Jing-Ru, Cheng Xiao-Ran, Zhang Qi, Zhao Ming-Hui. Functional analysis of OsST41 regulating salt tolerance in rice seedlings[J]. Acta Agronomica Sinica, 0, (): 0-.

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