关键词: 水稻突变体库, Rubisco小亚基和大亚基含量, 氧化胁迫, 筛选

Abstract: Sequencing of the rice genome is nearly complete; therefore, one of the most challenging goals now is to examine the function of its large number of genes. In this article, to get the details about the mechanism how Ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) can keep stable under oxidative stress, we screen the mutant of Rubisco using materials from a T-DNA transposon inserted rice （Oryza sativa L. ssp. japonica cv. Zhonghua 11）transgenic population, by comparing the contents of Rubisco small subunit and large subunit different from these of rice mutants treated with MV (Methyl viologen), a ROS inducer in vivo. We use SDS-PAGE to determine the content of subunits of Rubisco. Compared with the control, Rubisco from the leaves of the mutants had obvious difference in small subunit （SSU）content. We also determined mutants’ and the wild-type’s SPAD of leaves as a relative value of chlorophyll content, to compare the leaf senescence speed under oxidative stress. With this method we got two mutants whose Rubisco showed different tolerance to oxidative stress, demonstrating that this method of screening mutants is viable.

Key words: Rice mutant pool, SSU and LSU of Rubisco, Oxidative stress, Screening