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作物学报 ›› 2008, Vol. 34 ›› Issue (08): 1403-1408.doi: 10.3724/SP.J.1006.2008.01403

• 耕作栽培·生理生化 • 上一篇    下一篇

过量表达小麦超氧化物歧化酶(SOD)基因对烟草耐盐能力的影响

张海娜1;李小娟2;李存东1;肖凯1,*   

  1. 1河北农业大学农学院; 2河北农业大学生命科学学院, 河北保定071001
  • 收稿日期:2008-01-04 修回日期:1900-01-01 出版日期:2008-08-12 网络出版日期:2008-08-12
  • 通讯作者: 肖凯

Effects of Overexpression of Wheat Superoxide Dismutase (SOD) Genes on Salt Tolerant Capability in Tobacco

ZHANG Hai-Na1,LI Xiao-Juan2,LI Cun-Dong1,XIAO Kai1*   

  1. 1 College of Agronomy; 2 College of Life Science, Agricultural University of Hebei, Baoding 071001, Hebei, China
  • Received:2008-01-04 Revised:1900-01-01 Published:2008-08-12 Published online:2008-08-12
  • Contact: XIAO Kai

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1455

被引次数

97

摘要： 利用前期克隆的小麦超氧化物歧化酶(SOD)基因TaSOD1.1和TaSOD1.2, 构建融合上述基因编码阅读框(ORF)的双元表达载体。采用农杆菌介导的遗传转化技术, 获得了过量表达TaSOD1.1和TaSOD1.2的转基因烟草植株。研究表明, NaCl处理下, 与对照相比, 转基因植株伤害较小, 叶片失绿面积较少, 植株长势明显增强, 叶片的SOD活性均明显提高, 而MDA含量明显降低。转基因植株的叶绿素a、叶绿素b和类胡萝卜素含量, 以及可溶性糖和可溶性蛋白含量也表现与SOD活性相同的趋势。表明在烟草中高表达小麦TaSOD1.1和TaSOD1.2基因, 通过外源基因在转录水平上的调节, 能增加植株SOD活性, 减轻盐分胁迫造成的细胞膜质过氧化, 增强植株抵御盐分胁迫的能力。

关键词: 超氧化物歧化酶(SOD)基因, 遗传转化, 烟草, 耐盐能力

Abstract: For identification of the function of wheat novel superoxide dismutase (SOD) genes TaSOD1.1 and TaSOD1.2 in improving the salt tolerance, the transgenic tobacco plants with high expression levels of TaSOD1.1 and TaSOD1.2 were generated by the Agrobacterium tumerficians-mediated transformation approach. On NaCl treatment, the transgenic plants showed a better growth performance with less injured and more greenish leaves than control. The SOD activity was largely elevated in the tested transgenic tobacco plants than that in the control, so were the chlorophyll a, b contents, carotenoid content, soluble sugar content and soluble protein content. In the meantime, the MDA content was dramatically declined. Therefore, the transgenic tobacco plants with high expression levels of TaSOD1.1 and TaSOD1.2 could increase the SOD activity, alleviate the over-oxidation de-gree of cytoplasm membrane caused by NaCl stress, and enhance the NaCl tolerant capacity by the up-regulation of exotic target genes at the transcriptional level.

Key words: Superoxide dismutase (SOD) gene, Genetic transformation, Tobacco, Salt tolerant capacity

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