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作物学报 ›› 2009, Vol. 35 ›› Issue (4): 662-671.doi: 10.3724/SP.J.1006.2009.00662

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

温光敏核不育小麦C412S的育性转换及其APRT基因的表达

张建奎1;董静12;宗学凤1;余国东3;戴秀梅1;阮仁武1   

  1. 1西南大学农学与生物科技学院/农业部生物技术与作物品质改良重点开放实验室,重庆400715;2湖北省农业科学院,湖北武汉430064;3重庆市农业科学院,重庆402160
  • 收稿日期:2008-07-16 修回日期:2008-10-05 出版日期:2009-04-12 网络出版日期:2009-01-15
  • 基金资助:

    本研究由重庆市"十一五"科技攻关项目(CSTC2007AC1038),西南大学博士基金项目(99945-932001)资助

Fertility Alternation of Thermo-photo-sensitive Genic Male Sterile Wheat Line C412S and Expression of Fertility Related APRT Gene

ZHANG Jian-Kui1;DONG Jing12;ZONG Xue-Feng1;YU Guo-Dong3;DAI Xiu-Mei1;RUAN Ren-Wu1   

  1. 1College of Agronomy and Biotechnology/Key Laboratory of Biotechnology and Crop Ouality Improvement, Ministry of Agriculture, Southwest University,Chongqing 400716,China;2 Hubei Academy of Agricultural Sciences,Wuhan 430064,China;3 Chongqing Academy of Agricultural Sciences, Chongqing 4012160,China
  • Received:2008-07-16 Revised:2008-10-05 Published:2009-04-12 Published online:2009-01-15

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被引次数

23

摘要：

以选育的小麦(Triticum aestivum L.)温光敏核不育系C412S为试材，以C412S回交转育时的受体亲本C412为常规品系对照，通过分期播种试验研究了其育性转换特性。用涂抹压片法观察减数分裂和小孢子发育进程，用半定量RT-PCR技术分析不育与可育条件下不同发育时期的幼穗中腺嘌呤磷酸核糖基转移酶基因(APRT)的表达水平。结果表明，通过调整播种期改变雄性发育的温光条件，C412S表现出完全不育—高不育—半不育—正常可育的育性转换特性。C412S花粉败育的高峰在单核小孢子晚期，主要表现圆败型不育。C412S的育性敏感期是从花粉母细胞形成期到成熟花粉期，其中最敏感的时段是花粉母细胞形成期到减数分裂期。与对照相比，C412S的APRT1基因序列有个别碱基变异，但编码氨基酸序列没有变化。在花粉母细胞形成期至单核期，与晚播可育条件相比，早播不育的C412S幼穗中APRT基因转录水平下调，因此认为，其育性转换与幼穗中APRT基因转录水平有一定关系。

关键词: 小麦(Triticum aestivum L.), 温光敏细胞核雄性不育性(TGMS), 育性转换, 败育, APRT基因

Abstract:

C412S is a novel type of thermo-photo-sensitive genic male sterile (TGMS) wheat (Triticum aestivum L.) line, which is derived from the cross between TGMS line C49S and normal wheat line C412. To understand the fertility alternation of C412S, the courses of pollen mother cell (PMC) meiosis and microsporogenesis were observed using smear and squash microscopic techniques. Moreover, the expression characteristics of adenine phosphoribosyl-transferase gene (APRT) in young spikes were detected under sterile and fertile conditions using quantitative RT-PCR. When adjusting the environmental temperature and photoperiod at the formation and development stages of male sexual cell through series seeding dates, C412S showed continuous changes of fertility from complete sterility to high sterility, semi-sterility, lowe sterility, and normal fertility. C412S in normal PMC meiosis, however, showed a peak pollen abortion at later mononucleate stage of microsporogenesis, which mostly belonged to spherical abortion type. From PMC formation stage to mature pollen stage, C412S was sensitive in fertility, especially from PMC formation stage to PMC meiosis stage. Compared with C412 (control), C412S only had several base variations in a few loci of APRT1 gene, and the deducted amino acid sequences of C412S and C412 were completely identical. The RT-PCR analysis showed that the transcription level of APRT gene in the young spikes of sterile C412S plants was lower than that of fertile C412S plants from PMC formation stage to mono-nucleate microspore stage. Therefore, the transcription level of APRT gene in young spikes is probably related to the fertility alternation in TGMS wheat.

Key words: Wheat(Triticum aestivum L.), Thermo-photo-sensitive genic male sterility(TGMS), Fertility alternation APRT Gene

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