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作物学报 ›› 2009, Vol. 35 ›› Issue (7): 1274-1281.doi: 10.3724/SP.J.1006.2009.01274

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦抗条锈病基因YrZH84的RGAP标记及其应用

殷贵鸿1,2,3,王建武1,2,闻伟锷2,何中虎2,4,李在峰5,王辉1,夏先春2,*   

  1. 1西北农林科技大学农学院,陕西杨凌712100;2中国农业科学院作物科学研究所/国家小麦改良中心/高家农作物基因资源与基因改良重大科学工程,北京100081;3周口市农业科学院,河南周口466001;4CIMMYT中国办事处,北京100081;5河北农业大学植物保护学院,河北保定071001
  • 收稿日期:2008-12-26 修回日期:2009-03-23 出版日期:2009-07-12 网络出版日期:2009-05-19
  • 通讯作者: 夏先春, Tel: 010-68918610, E-mail: xiaxianchun@caas.net.cn
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2006AA10Z1A7和2006AA100102)资助。

Mapping of Wheat Stripe Rust Resistance Gene YrZH84 with RGAP Markers and Its Application

YIN Gui-Hong1,2,3,WANG Jian-Wu1,2,WEN Wei-E2,HE Zhong-Hu2,4,LI Zai-Feng5,WANG Hui1,XIA Xian-Chun2*   

  1. 1College of Agronomy,Northwest A&F University,Yangling 712100,China;2Institute of Crop Sciences/National Wheat Improvement Center/National Key Facility for Crop Gene Resources and Genetic Improvement,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3Zhoukou Academy of Agricultural Sciences,Zhoukou 466001,China;4CIMMYT China Office,Beijing 100081;5College of Plant Protection,Agricultural University of Hebei,Baoding 071001,China
  • Received:2008-12-26 Revised:2009-03-23 Published:2009-07-12 Published online:2009-05-19
  • Contact: XIA Xian-Chun, Tel: 010-68918610, E-mail: xiaxianchun@caas.net.cn

摘要:

利用RGAP标记及基于标记基因型和表型选择的分离群体分组分析法,对小麦抗条锈病骨干亲本周8425B和感病品种中国春及其F2分离群体进行分析,获得了与抗条锈基因YrZH84紧密连锁的RGAP标记Xrga-1,连锁距离为0.8 cMRGA片段长度为343 bpBLAST分析结果表明,该RGA序列与已克隆的大麦抗秆锈病基因Rpg1核苷酸序列同源性为93%,与大麦抗白粉病基因Mla家族的核苷酸序列同源性为92%。用标记Xrga-1对黄淮麦区58个小麦品种()进行了检测,结合系谱分析和抗病性鉴定结果,发现周8425B的衍生品种周麦11、周麦17、周麦20、周麦22、矮抗580436、源育3号、0537、宛抗18、豫展10号携带抗条锈病基因YrZH84。这些研究结果对小麦抗条锈病分子育种和YrZH84基因克隆有重要作用。

关键词: 普通小麦, 抗条锈病基因, RGAP标记

Abstract:

Zhou 8425B, with an effective stripe rust resistance gene YrZH84, is a hall-mark parent line of wheat (Triticum aestivum L.) in the Yellow-Huai rivers wheat growing region. An F2 population derived from the cross Zhou 8425B/Chinese Spring and their parents were used forpolymorphism analysis employing the resistance gene-analog polymorphism (RGAP) markers and bulked segregant analysis (BSA) based on marker genotypes and phenotypes selection. An RGAP marker Xrga-1 was identified, which was tightly linked to the stripe rust resistance gene YrZH84, with a genetic distance of 0.8 cM. The fragment amplified by Xrga-1 was 343 bp. BLAST analysis indicated that the rga-1 showed 93% of identical nucleotide sequences to barley stem rust resistance gene Rpg1, and 92% to barley powdery mildew resistance gene Mla homology family. Fifty-eight cultivars from Yellow-Huai rivers wheat growing region were genotyped by the RGAP marker Xrga-1. From the information of genotypes, pedigrees and resistance responses, the cultivars Zhoumai 11, Zhoumai 20, Zhoumai 22, Aikang 58, 04 Zhong 36, Yuanyu 3, 05 Zhong 37, Wuankang 18, and Yuzhan 10 were identified to have the resistance gene YrZH84. These cultivars were the derivatives of Zhou 8425B. These results will greatly benefit the marker-assisted selection in wheat and cloning of the resistance gene YrZH84.

Key words: Common wheat, Stripe rust resistance gene, RGAP marker

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