作物学报 ›› 2009, Vol. 35 ›› Issue (10): 1778-1790.doi: 10.3724/SP.J.1006.2009.01778
廖亮1,2,李同建2,刘中来2,邓辉胜2,徐玲玲2,*,潘其辉3,赖占均3,石庆华1,*
LIAO Liang1,2,LI Tong-Jian2,LIU Zhong-Lai2,DENG Hui-Sheng2,XU Ling-Ling2,*,PAN Qi-Hui3,LAI Zhan-Jun3,SHI Qing-Hua1*
摘要:
为了了解苎麻与野生近缘种的系统学关系,更有效利用野生苎麻资源,通过细胞学观察和分子标记证据,对中国苎麻与其24个野生近缘类群进行了系统学分析。细胞学结果表明,苎麻间期核形态属于田中分类体系的前染色体型,而其野生近缘种分别属于前染色体型和分散型;苎麻和23个野生近缘物种(其中15个类群的染色体数目为首次报道)中,19个为二倍体(2n=28),而大叶苎麻组3个类群为三倍体(2n=42),1个为四倍体(2n=56),1个为五倍体(2n=70)。ITS序列和trnL-F序列分析表明,苎麻属可分成A(含A1和A2亚分支)、B(含B1和B2亚分支)和C 3个基本分支。根据分析结果本文提出苎麻属的两条演化路线: (1)前染色体型进化路线A1→A2,即腋球苎麻组→苎麻组;(2)分散型进化路线B→C,即腋球苎麻组→帚序苎麻组→序叶苎麻组→大叶苎麻组。
[1] Lai Z-J(赖占钧). The Grass Linen of Jiangxi(江西夏布). Beijing: Scientific and Technologic Literature Publishing House, 2002 (in Chinese)[2] Wang W-C(王文采). Revisio Boehmeriae Sinicae. Acta Bot Yunnanica (云南植物研究), 1981, 3(3): 307-328 (in Chinese)[3] Wang W-C(王文采). Revisio Boehmeriae Sinicae (Cont.). Acta Bot Yunnanica (云南植物研究), 1981, 3(4): 401-416 (in Chinese)[4] Wang W-C(王文采). Boehmeria中国植物志). Beijing: Science Press, 1995, 23(2): 187-312 (in Chinese) . In: Flora Reipublicae Popularis Sinicae ([5] Chen J-R(陈家瑞), Friis I. Boehmeria. In: Flora of China (中国植物志).Beijing: Science Press, 2003. pp 164-174 (in Chinese)[6] Satake Y. Boehmeria japonica Journ. Sci Univ Tokyo Sect III, 1936, 4: 467-542[7] Zhang B(张波), Zheng C-Q(郑长清). Classification and evolution of group on the Boehmeria in China. 作物学报), 1998, 24(6): 775-781 (in Chinese with English abstract)Acta Agron Sin)[8] Zhang B(张波), Zheng C-Q(郑长清), Zang G-G(臧巩固), Zhao L-N(赵立宁). The comparative morphology of Boehmeria in China. Sci Agric Sin (中国农业科学), 1998, 31(2): 36-10 (in Chinese with English abstract)[9] Zhang B(张波), Zuo Z-M(左志明). On cytology study of wild Raim and relatives species in Guangxi. Guangxi Agric Sci (广西农业科学), 1996, (2): 73-77 (in Chinese)[10] Zang G-G(臧巩固). A karyological study on five species in three section of genus Boehmeria中国麻作), 1993, (1): 1-6 (in Chinese with English abstract) . China’s Fiber Crops ([11] Goldblatt P, Johnson D E. Index to Plant Chromosome Numbers. Available at http://mobot.mobot.org/W3T/Search/ipcn.html[12] Tanaka R. Types of resting nuclei in orchidaceae. Bot Mag (Tokyo), 1971, 84: 118-122[13] Tanaka R. The karyotype theory and wide crossing as an example in Orchidaceae. In: Hong D Y ed. Plant Chromosome Research. Hiroshima: Proceedings of the Sino-Japanese Symposium on Plant Chromosomes, 1989. pp 1-10[14] Li M-X(李懋学). Plant Chromosome Research Technique (植物染色体研究技术). Changchun: Northeast Forestry University Press, 1991. pp 146-148 (in Chinese)[15] Guo A-P(郭安平). Phylogeny of Genus Boehmerla Jacq. Reconstructed by RAPD Fingerprinting. PhD Dissertation of Hunan Agricultural University, 1999 (in Chinese with English abstract)[16] Doyle J J, Doyle J L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull, 1987, 19: 11-15[17] White T J, Bruns T, Lee S, Taylor J W. Amplification and direct sequencing of fungal ribosomal RNA genesfor phylogenetics. In: Innis M A, Gelfand D H, Sninsky J J, White T J, eds. PCR Protocols: A Guide to Methods and Applications. San Diego: Academic Press. 1990. pp 315-322[18] Taberlet P T, Gielly L, Patou G, Bouvet J. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol, 1991, 17: 1105-1109[19] Joseph S, Rusell D W, eds. Huang P-T(黄培堂) trans. Molecular Cloning: A Laboratory Manual, 3rd edn (分子克隆实验指南·第三版). Beijing: Science Press, 2002. pp 2-137 (in Chinese)[20] Thompson J D, Gibson T J, Plewinak F, Jeanmougin F, Higgins D G. The Clustal-X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res, 1997, 25: 4876-4882[21] Swofford D L. PAUP4.0b10. Phylogenetic analysis using parsimony (and other methods). Ver 4. Sunderland, MA: Sinauer Associates, 2002[22] Huelsenbeck J P, Ronquist F. MrBayes. Bioinformatics, 2001, 17: 754[23] Nylander J A. MrModeltest ver 2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, 2004[24] Chen S-C(陈士超), Yang H(杨红), Li S(李珊), Zhu J(祝建), Fu C-X(傅承新). Bayesian inference and its application in the molecular phylogeny of liliales. Acta Bot Yunnanica (云南植物研究), 2007,29(2): 161-166 (in Chinese with English abstract)Zhang Y(张原), Zhang Z-W(张正旺), Zheng N(郑楠). The application of likelihood ratio tests and Bayesian inference in the molecular phylogeny of Phasianidae. J Beijing Norm Univ (Nat Sci) (北京师范大学学报·自然科学版), 2003, 39(2): 250-257 (in Chinese with English abstract) |
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