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作物学报 ›› 2009, Vol. 35 ›› Issue (10): 1771-1777.doi: 10.3724/SP.J.1006.2009.01771

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

根癌农杆菌介导转Bt基因苎麻的获得及其抗虫鉴定

符家平，汪波**，刘立军，杨金雨，王绪霞，邢秀龙，彭定祥*   

  1. 华中农业大学植物科技学院麻类研究室，湖北武汉430070
  • 收稿日期:2009-03-03 修回日期:2009-06-25 出版日期:2009-10-12 网络出版日期:2009-08-07
  • 通讯作者: 彭定祥，E-mail:pdxiang@mail.hzau.edu.cn; Tel:13397121298
  • 基金资助:

    本研究由公益性行业（农业）科研专项（nyhyzx07-018-16），国家自然科学基金项目（30800696），教育部博士点新教师基金项目（20070504059）资助。

Transgenic Ramie with Bt Gene  Mediated by Agrobacterium tumefacien and Evaluation of Its Pest-Resistance

FU Jia-Ping,WANG Bo**,LIU Li-Jun,YANG Jin-Yu,WANG Xu-Xia,XING Xiu-Long,PENG Ding-Xiang*   

  1. College of Plant Sciences and Technology，Huazhong Agricultural University，Wuhan 430070，China
  • Received:2009-03-03 Revised:2009-06-25 Published:2009-10-12 Published online:2009-08-07
  • Contact: PENG Ding-Xiang，E-mail: pdxiang@mail.hzau.edu.cn; Tel: 13397121298

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1902

被引次数

8

摘要：

采用本研究室建立的根癌农杆菌介导的高效遗传转化体系，转化苎麻优良品种芦竹青，获得了转Bt基因苎麻候选植株。通过PCR和Southern杂交等分子检测，证明Bt基因已经整合到部分候选植株基因组中。选取PCR和Southern杂交均为阳性的部分株系(T0)种植在大田，对这些植株进行室内抗虫鉴定并考察其主要农艺性状和品质性状，次年对T1代植株进行PCR和Southern杂交检测。结果表明, 与对照相比，T0代转Bt基因苎麻植株的抗虫性均强于对照，部分株系的抗虫性显著强于对照植株；且基本保持了亲本的优良性状；T1代植株中也含有Bt基因，表明Bt基因能稳定遗传，且T1代植株在大田的抗虫性明显强于非转基因植株。

关键词: 苎麻, 转基因, Bt基因, 赤蛱蝶, 抗虫

Abstract:

Agrobacterium tumefaciens-mediated approach is an effectively and widely used to introduce foreign DNA into plants. However, there were few reports published on ramie transformation mediated by Agrobacterium tumefaciens up to date. In the present study, transgenic ramie containing Bt gene were obtained by our highly efficient Agrobacterium-mediated transformation system using ramie Luzhuqing. Three T0 plants of transgenic ramie were chosen for further experiments and were transplanted into the fields. Laboratory assay of artificial infestation and the survey on agronomic traits and quality traits of T0 plants were conducted. The results showed that T0 transgenic plants exhibited more excellent pest resistance than the controls, and some were significantly superior to the controls. In addition, T0 transgenic plants maintained the main agronomic traits, such as plant height, stem diameter, rate of effective tiller, raw fiber weight, fiber production ratio from stem and fiber production ratio from phloem, and quality traits, such as strength, filament breaking tenacity, fiber fineness, fineness uniformity and glue content of raw fiber in Luzhuqing. PCR and Southern blotting analysis showed that T1 plants contained Bt gene, which indicated that Bt gene could be stably inherited. T1 plants in the fields performed highly pest resistance compared with non-transgenic plants.

Key words: Ramie, Transformation, Bt gene, Pyrameis indica（HerBst）, Agrobaterium tumefaciens

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