作物学报 ›› 2024, Vol. 50 ›› Issue (8): 1971-1988.doi: 10.3724/SP.J.1006.2024.31080
高维东1,2(), 胡城祯1,2, 张龙1,2, 张艳艳1,2, 张沛沛1, 杨德龙1,2,*(
), 陈涛1,2,*(
)
GAO Wei-Dong1,2(), HU Chen-Zhen1,2, ZHANG Long1,2, ZHANG Yan-Yan1,2, ZHANG Pei-Pei1, YANG De-Long1,2,*(
), CHEN Tao1,2,*(
)
摘要:
E2泛素结合酶在调控植物生长发育和胁迫信号转导过程中发挥着重要作用。本研究以小麦抗旱品种晋麦47的cDNA为模板克隆出E2泛素结合酶TaUBC16, 该基因全长447 bp, 编码148个氨基酸。顺式作用元件分析发现, TaUBC16启动子区含有与分生组织发育、胁迫响应、植物激素应答相关的多种顺式作用元件。利用小麦RNA-Seq转录组数据结合qRT-PCR验证分析发现, TaUBC16在小麦不同组织器官和发育阶段普遍表达, 其中在30 d籽粒中的表达量较高, 且均能被PEG-6000、甘露醇和ABA显著诱导表达。烟草叶片和小麦原生质体亚细胞定位分析表明, TaUBC16蛋白分布于细胞质和细胞核。通过异源表达TaUBC16转基因拟南芥进行生长发育表型分析发现, 转基因株系开花时间早于野生型, 其籽粒相比于野生型更为饱满, 千粒重显著高于野生型。基于启动子区-388 bp位点(T-A)的多态性, 开发了TaUBC16基因的竞争性等位基因特异性PCR (kompetitive allele-specific PCR, KASP)标记, 鉴定了TaUBC16的单倍型, 发现TaUBC16-Hap I的千粒重、粒长和粒宽显著高于TaUBC16-Hap II, 并在我国小麦育种进程中得到正向选择。本研究结果将为进一步揭示TaUBC16基因参与调控小麦生长发育和响应逆境胁迫分子机理提供理论依据。
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